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P49656 (IRK2_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Inward rectifier potassium channel 2
Alternative name(s):
Inward rectifier K(+) channel Kir2.1
Short name=IRK-1
Potassium channel, inwardly rectifying subfamily J member 2
Gene names
Name:KCNJ2
Synonyms:IRK1
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.

Subunit structure

Homomultimeric and heteromultimeric association with Kir2.3, resulting in an enhanced G-protein-induced current. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Highly expressed in the ventricle and skeletal muscle, moderately in cerebrum and cerebellum. Only low levels are detected in kidney or lung.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ2 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427Inward rectifier potassium channel 2
PRO_0000154927

Regions

Topological domain1 – 8181Cytoplasmic By similarity
Transmembrane82 – 10625Helical; Name=M1; By similarity
Topological domain107 – 12822Extracellular By similarity
Intramembrane129 – 14012Helical; Pore-forming; Name=H5; By similarity
Intramembrane141 – 1477Pore-forming; By similarity
Topological domain148 – 1569Extracellular By similarity
Transmembrane157 – 17822Helical; Name=M2; By similarity
Topological domain179 – 427249Cytoplasmic By similarity
Motif142 – 1476Selectivity filter By similarity
Motif425 – 4273PDZ-binding Potential

Sites

Site1721Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
P49656 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 1A57A8BB19B8A4A3

FASTA42748,350
        10         20         30         40         50         60 
MGSVRTNRYS IVSSEEDGMK LATMAVANGF GNGKSKVHTR QQCRSRFVKK DGHCNVQFIN 

        70         80         90        100        110        120 
VGEKGQRYLA DIFTTCVDIR WRWMLVIFCL AFVLSWLFFG CVFWLIALLH GDLDASRESK 

       130        140        150        160        170        180 
ACVSEVNSFT AAFLFSIETQ TTIGYGFRCV TDECPVAVFM VVFQSIVGCI IDAFIIGAVM 

       190        200        210        220        230        240 
AKMAKPKKRN ETLVFSHNAV IAMRDGKLCL MWRVGNLRKS HLVEAHVRAQ LLKSRITSEG 

       250        260        270        280        290        300 
EYIPLDQIDI NVGFDSGIDR IFLVSPITIV HEIDEDSPLY DLSKQDMDNA DFEIVVILEG 

       310        320        330        340        350        360 
MVEATAMTTQ CRSSYLANEI LWGHRYEPVL FEEKHYYKVD YSRFHKTYEV PNTPLCSARD 

       370        380        390        400        410        420 
LAEKKYILSN ANSFCYENEV ALTSKEEDDS ENGVPESTST DTPPDIDLHN QASVPLEPRP 


LRRESEI

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References

[1]"Cloning and functional expression of a cardiac inward rectifier K+ channel."
Ishii K., Yamagishi T., Taira N.
FEBS Lett. 338:107-111(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: New Zealand white.
Tissue: Heart.
[2]"Inwardly rectifying potassium channels in lens epithelium are from the IRK1 (Kir 2.1) family."
Rae J.L., Shepard A.R.
Exp. Eye Res. 66:347-359(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: New Zealand white.
Tissue: Lens epithelium.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D21057 mRNA. Translation: BAA04636.1.
AF021138 mRNA. Translation: AAB88796.1.
PIRS41087.
RefSeqNP_001075667.1. NM_001082198.1.
UniGeneOcu.2469.

3D structure databases

ProteinModelPortalP49656.
SMRP49656. Positions 187-370.
ModBaseSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000008971.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSOCUT00000010415; ENSOCUP00000008971; ENSOCUG00000010416.
GeneID100008987.

Organism-specific databases

CTD3759.

Phylogenomic databases

eggNOGNOG72812.
GeneTreeENSGT00650000093228.
HOGENOMHOG000237325.
HOVERGENHBG006178.
OMAHNATVAM.
OrthoDBEOG4N04DV.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003271. K_chnl_inward-rec_Kir2.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF15. PTHR11767:SF15. 1 hit.
PfamPF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01324. KIR21CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. Ig_E-set. 1 hit.
ProtoNetSearch...

Entry information

Entry nameIRK2_RABIT
AccessionPrimary (citable) accession number: P49656
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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