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P49655 (KCJ10_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-sensitive inward rectifier potassium channel 10
Alternative name(s):
ATP-sensitive inward rectifier potassium channel KAB-2
BIR10
Brain-specific inwardly rectifying K(+) channel 1
Short name=BIRK1
Inward rectifier K(+) channel Kir4.1
Potassium channel, inwardly rectifying subfamily J member 10
Gene names
Name:Kcnj10
Synonyms:Kab-2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.

Subunit structure

Interacts with INADL By similarity. Seems to form heterodimer with Kir5.1/KCNJ16.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Predominantly in glial cells of the cerebellum and forebrain. Expressed to a lesser extent in the kidney, and other peripheral tissues.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
Potassium
   Molecular functionIon channel
Voltage-gated channel
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-glutamate import

Inferred from mutant phenotype PubMed 17091490. Source: RGD

L-glutamate uptake involved in synaptic transmission

Inferred from electronic annotation. Source: Ensembl

adult walking behavior

Inferred from electronic annotation. Source: Ensembl

central nervous system myelination

Inferred from electronic annotation. Source: Ensembl

inflammatory response

Inferred from expression pattern PubMed 15748953. Source: RGD

membrane hyperpolarization

Inferred from mutant phenotype PubMed 17091490. Source: RGD

optic nerve development

Inferred from expression pattern PubMed 15198689. Source: RGD

potassium ion homeostasis

Inferred from electronic annotation. Source: Ensembl

potassium ion import

Inferred from mutant phenotype PubMed 17091490. Source: RGD

protein homotetramerization

Inferred from direct assay PubMed 16206160. Source: RGD

regulation of long-term neuronal synaptic plasticity

Inferred from electronic annotation. Source: Ensembl

regulation of resting membrane potential

Inferred from electronic annotation. Source: Ensembl

regulation of sensory perception of pain

Inferred from mutant phenotype PubMed 18417695. Source: RGD

response to blue light

Inferred from expression pattern PubMed 18450590. Source: RGD

response to glucocorticoid

Inferred from expression pattern PubMed 20466875. Source: RGD

response to mineralocorticoid

Inferred from expression pattern PubMed 20466875. Source: RGD

visual perception

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentapical plasma membrane

Inferred from direct assay PubMed 18395087. Source: RGD

basolateral plasma membrane

Inferred from direct assay PubMed 18303016. Source: RGD

dystrophin-associated glycoprotein complex

Inferred from direct assay PubMed 16206160. Source: RGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

microvillus

Inferred from direct assay PubMed 20466875. Source: RGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

identical protein binding

Inferred from direct assay PubMed 16206160. Source: RGD

inward rectifier potassium channel activity

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 19931615. Source: UniProtKB

receptor binding

Inferred from physical interaction PubMed 21084311. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 379379ATP-sensitive inward rectifier potassium channel 10
PRO_0000154955

Regions

Topological domain1 – 6464Cytoplasmic By similarity
Transmembrane65 – 8925Helical; Name=M1; By similarity
Topological domain90 – 11425Extracellular By similarity
Intramembrane115 – 12612Helical; Pore-forming; Name=H5; By similarity
Intramembrane127 – 1337Pore-forming; By similarity
Topological domain134 – 1429Extracellular By similarity
Transmembrane143 – 16422Helical; Name=M2; By similarity
Topological domain165 – 379215Cytoplasmic By similarity
Nucleotide binding210 – 2178ATP Potential
Motif128 – 1336Selectivity filter By similarity

Sites

Site1581Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Experimental info

Sequence conflict731A → P in AAA87811. Ref.3
Sequence conflict761A → T in AAA87811. Ref.3
Sequence conflict110 – 1123VQV → GAGA Ref.3
Sequence conflict115 – 1162LT → YL Ref.3
Sequence conflict1451V → E in AAA87811. Ref.3
Sequence conflict1901H → L in AAA87811. Ref.3
Sequence conflict2281N → D in AAA87811. Ref.3
Sequence conflict2661K → Q in AAA87811. Ref.3
Sequence conflict3041E → R in CAA58568. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P49655 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 77B3671907B68CB2

FASTA37942,480
        10         20         30         40         50         60 
MTSVAKVYYS QTTQTESRPL VAPGIRRRRV LTKDGRSNVR MEHIADKRFL YLKDLWTTFI 

        70         80         90        100        110        120 
DMQWRYKLLL FSATFAGTWF LFGVVWYLVA VAHGDLLELG PPANHTPCVV QVHTLTGAFL 

       130        140        150        160        170        180 
FSLESQTTIG YGFRYISEEC PLAIVLLIAQ LVLTTILEIF ITGTFLAKIA RPKKRAETIR 

       190        200        210        220        230        240 
FSQHAVVAYH NGKLCLMIRV ANMRKSLLIG CQVTGKLLQT HQTKEGENIR LNQVNVTFQV 

       250        260        270        280        290        300 
DTASDSPFLI LPLTFYHVVD ETSPLKDLPL RSGEGDFELV LILSGTVEST SATCQVRTSY 

       310        320        330        340        350        360 
LPEEILWGYE FTPAISLSAS GKYVADFSLF DQVVKVASPG GLRDSTVRYG DPEKLKLEES 

       370 
LREQAEKEGS ALSVRISNV 

« Hide

References

[1]"A novel ATP-dependent inward rectifier potassium channel expressed predominantly in glial cells."
Takumi T., Ishii T., Horio Y., Morishige K., Takahashi N., Yamada M., Yamashita T., Kiyama H., Sohmiya K., Nakanishi S., Kurachi Y.
J. Biol. Chem. 270:16339-16346(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Cloning and expression of a family of inward rectifier potassium channels."
Bond C.T., Pessia M., Xia X.-M., Lagrutta A., Kavanaugh M.P., Adelman J.P.
Recept. Channels 2:183-191(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[3]"Cloning and expression of two brain-specific inwardly rectifying potassium channels."
Bredt D.S., Wang T.L., Cohen N.A., Guggino W.B., Snyder S.H.
Proc. Natl. Acad. Sci. U.S.A. 92:6753-6757(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X86818 mRNA. Translation: CAA60501.1.
X83585 mRNA. Translation: CAA58568.1.
U27558 mRNA. Translation: AAA87811.1.
PIRA57477.
RefSeqNP_113790.1. NM_031602.1.
UniGeneRn.10196.

3D structure databases

ProteinModelPortalP49655.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248335. 2 interactions.
IntActP49655. 3 interactions.
MINTMINT-103719.
STRING10116.ENSRNOP00000010146.

Chemistry

GuidetoPHARMACOLOGY438.

PTM databases

PhosphoSiteP49655.

Proteomic databases

PaxDbP49655.
PRIDEP49655.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000010146; ENSRNOP00000010146; ENSRNOG00000007705.
GeneID29718.
KEGGrno:29718.
UCSCRGD:61822. rat.

Organism-specific databases

CTD3766.
RGD61822. Kcnj10.

Phylogenomic databases

eggNOGNOG284842.
GeneTreeENSGT00690000102074.
HOGENOMHOG000237326.
HOVERGENHBG006178.
InParanoidP49655.
KOK05003.
OMAFYHVVDD.
OrthoDBEOG7GFB4Z.
PhylomeDBP49655.
TreeFamTF313676.

Gene expression databases

GenevestigatorP49655.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003269. K_chnl_inward-rec_Kir1.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF21. PTHR11767:SF21. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01322. KIR12CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio610177.
PROP49655.

Entry information

Entry nameKCJ10_RAT
AccessionPrimary (citable) accession number: P49655
Secondary accession number(s): Q62790
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families