P49655 (IRK10_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-sensitive inward rectifier potassium channel 10

Alternative name(s):
ATP-sensitive inward rectifier potassium channel KAB-2
BIR10
Brain-specific inwardly rectifying K(+) channel 1
Short name=BIRK1
Inward rectifier K(+) channel Kir4.1
Potassium channel, inwardly rectifying subfamily J member 10

Gene names

Name:	Kcnj10
Synonyms:	Kab-2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.
Subunit structure	Interacts with INADL By similarity. Seems to form heterodimer with Kir5.1/KCNJ16.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Predominantly in glial cells of the cerebellum and forebrain. Expressed to a lesser extent in the kidney, and other peripheral tissues.
Sequence similarities	Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding Potassium
Molecular function	Ion channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-glutamate import Inferred from mutant phenotype PubMed 17091490. Source: RGD L-glutamate uptake involved in synaptic transmission Inferred from electronic annotation. Source: Compara adult walking behavior Inferred from electronic annotation. Source: Compara central nervous system myelination Inferred from electronic annotation. Source: Compara inflammatory response Inferred from expression pattern PubMed 15748953. Source: RGD membrane hyperpolarization Inferred from mutant phenotype PubMed 17091490. Source: RGD optic nerve development Inferred from expression pattern PubMed 15198689. Source: RGD potassium ion homeostasis Inferred from electronic annotation. Source: Compara potassium ion import Inferred from mutant phenotype PubMed 17091490. Source: RGD protein homotetramerization Inferred from direct assay PubMed 16206160. Source: RGD regulation of long-term neuronal synaptic plasticity Inferred from electronic annotation. Source: Compara regulation of resting membrane potential Inferred from electronic annotation. Source: Compara regulation of sensory perception of pain Inferred from mutant phenotype PubMed 18417695. Source: RGD response to blue light Inferred from expression pattern PubMed 18450590. Source: RGD response to glucocorticoid stimulus Inferred from expression pattern PubMed 20466875. Source: RGD response to mineralocorticoid stimulus Inferred from expression pattern PubMed 20466875. Source: RGD visual perception Inferred from electronic annotation. Source: Compara
Cellular_component	apical plasma membrane Inferred from direct assay PubMed 18395087. Source: RGD basolateral plasma membrane Inferred from direct assay PubMed 18303016. Source: RGD dystrophin-associated glycoprotein complex Inferred from direct assay PubMed 16206160. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microvillus Inferred from direct assay PubMed 20466875. Source: RGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from direct assay PubMed 16206160. Source: RGD inward rectifier potassium channel activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	ATP-sensitive inward rectifier potassium channel 10		PRO_0000154955
Regions
	Topological domain	1 – 64	64	Cytoplasmic By similarity
	Transmembrane	65 – 89	25	Helical; Name=M1; By similarity
	Topological domain	90 – 114	25	Extracellular By similarity
	Intramembrane	115 – 126	12	Helical; Pore-forming; Name=H5; By similarity
	Intramembrane	127 – 133	7	Pore-forming; By similarity
	Topological domain	134 – 142	9	Extracellular By similarity
	Transmembrane	143 – 164	22	Helical; Name=M2; By similarity
	Topological domain	165 – 379	215	Cytoplasmic By similarity
	Nucleotide binding	210 – 217	8	ATP Potential
	Motif	128 – 133	6	Selectivity filter By similarity
Sites
	Site	158	1	Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity
Experimental info
	Sequence conflict	73	1	A → P in AAA87811. Ref.3
	Sequence conflict	76	1	A → T in AAA87811. Ref.3
	Sequence conflict	110 – 112	3	VQV → GAGA Ref.3
	Sequence conflict	115 – 116	2	LT → YL Ref.3
	Sequence conflict	145	1	V → E in AAA87811. Ref.3
	Sequence conflict	190	1	H → L in AAA87811. Ref.3
	Sequence conflict	228	1	N → D in AAA87811. Ref.3
	Sequence conflict	266	1	K → Q in AAA87811. Ref.3
	Sequence conflict	304	1	E → R in CAA58568. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P49655 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 77B3671907B68CB2	FASTA	379	42,480
	10 20 30 40 50 60 MTSVAKVYYS QTTQTESRPL VAPGIRRRRV LTKDGRSNVR MEHIADKRFL YLKDLWTTFI 70 80 90 100 110 120 DMQWRYKLLL FSATFAGTWF LFGVVWYLVA VAHGDLLELG PPANHTPCVV QVHTLTGAFL 130 140 150 160 170 180 FSLESQTTIG YGFRYISEEC PLAIVLLIAQ LVLTTILEIF ITGTFLAKIA RPKKRAETIR 190 200 210 220 230 240 FSQHAVVAYH NGKLCLMIRV ANMRKSLLIG CQVTGKLLQT HQTKEGENIR LNQVNVTFQV 250 260 270 280 290 300 DTASDSPFLI LPLTFYHVVD ETSPLKDLPL RSGEGDFELV LILSGTVEST SATCQVRTSY 310 320 330 340 350 360 LPEEILWGYE FTPAISLSAS GKYVADFSLF DQVVKVASPG GLRDSTVRYG DPEKLKLEES 370 LREQAEKEGS ALSVRISNV

References

[1]	"A novel ATP-dependent inward rectifier potassium channel expressed predominantly in glial cells." Takumi T., Ishii T., Horio Y., Morishige K., Takahashi N., Yamada M., Yamashita T., Kiyama H., Sohmiya K., Nakanishi S., Kurachi Y. J. Biol. Chem. 270:16339-16346(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"Cloning and expression of a family of inward rectifier potassium channels." Bond C.T., Pessia M., Xia X.-M., Lagrutta A., Kavanaugh M.P., Adelman J.P. Recept. Channels 2:183-191(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[3]	"Cloning and expression of two brain-specific inwardly rectifying potassium channels." Bredt D.S., Wang T.L., Cohen N.A., Guggino W.B., Snyder S.H. Proc. Natl. Acad. Sci. U.S.A. 92:6753-6757(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X86818 mRNA. Translation: CAA60501.1. X83585 mRNA. Translation: CAA58568.1. U27558 mRNA. Translation: AAA87811.1.
IPI	IPI00231208.
PIR	A57477.
RefSeq	NP_113790.1. NM_031602.1.
UniGene	Rn.10196.
3D structure databases
ProteinModelPortal	P49655.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49655. 1 interaction.
MINT	MINT-103719.
STRING	10116.ENSRNOP00000010146.
PTM databases
PhosphoSite	P49655.
Proteomic databases
PaxDb	P49655.
PRIDE	P49655.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000010146; ENSRNOP00000010146; ENSRNOG00000007705.
GeneID	29718.
KEGG	rno:29718.
UCSC	RGD:61822. rat.
Organism-specific databases
CTD	3766.
RGD	61822. Kcnj10.
Phylogenomic databases
eggNOG	NOG284842.
GeneTree	ENSGT00690000102074.
HOGENOM	HOG000237326.
HOVERGEN	HBG006178.
InParanoid	P49655.
KO	K05003.
OMA	LGPPANH.
OrthoDB	EOG483D4R.
Gene expression databases
Genevestigator	P49655.
GermOnline	ENSRNOG00000007705. Rattus norvegicus.
Family and domain databases
Gene3D	2.60.40.1400. 1 hit.
InterPro	IPR014756. Ig_E-set. IPR016449. K_chnl_inward-rec_Kir. IPR003269. K_chnl_inward-rec_Kir1.2. IPR013518. K_chnl_inward-rec_Kir_cyto. [Graphical view]
PANTHER	PTHR11767. PTHR11767. 1 hit. PTHR11767:SF21. PTHR11767:SF21. 1 hit.
Pfam	PF01007. IRK. 1 hit. [Graphical view]
PIRSF	PIRSF005465. GIRK_kir. 1 hit.
PRINTS	PR01322. KIR12CHANNEL. PR01320. KIRCHANNEL.
SUPFAM	SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...
Other
NextBio	610177.

Entry information

Entry name

IRK10_RAT

Accession

Primary (citable) accession number: P49655
Secondary accession number(s): Q62790

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program