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Protein

ATP-sensitive inward rectifier potassium channel 10

Gene

Kcnj10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. In the kidney, together with KCNJ16, mediates basolateral K+ recycling in distal tubules; this process is critical for Na+ reabsorption at the tubules (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei158 – 1581Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi210 – 2178ATPSequence analysis

GO - Molecular functioni

  • ATP-activated inward rectifier potassium channel activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: RGD
  • receptor binding Source: RGD

GO - Biological processi

  • inflammatory response Source: RGD
  • L-glutamate import Source: RGD
  • membrane hyperpolarization Source: RGD
  • optic nerve development Source: RGD
  • potassium ion import Source: RGD
  • protein homotetramerization Source: RGD
  • regulation of sensory perception of pain Source: RGD
  • response to blue light Source: RGD
  • response to glucocorticoid Source: RGD
  • response to mineralocorticoid Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-sensitive inward rectifier potassium channel 10
Alternative name(s):
ATP-sensitive inward rectifier potassium channel KAB-2
BIR10
Brain-specific inwardly rectifying K(+) channel 1
Short name:
BIRK1
Inward rectifier K(+) channel Kir4.1
Potassium channel, inwardly rectifying subfamily J member 10
Gene namesi
Name:Kcnj10
Synonyms:Kab-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61822. Kcnj10.

Subcellular locationi

  • Membrane By similarity; Multi-pass membrane protein By similarity
  • Basolateral cell membrane By similarity

  • Note: In kidney distal convoluted tubules, located in the basolateral membrane where it colocalizes with KCNJ16.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6464CytoplasmicBy similarityAdd
BLAST
Transmembranei65 – 8925Helical; Name=M1By similarityAdd
BLAST
Topological domaini90 – 11425ExtracellularBy similarityAdd
BLAST
Intramembranei115 – 12612Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei127 – 1337Pore-formingBy similarity
Topological domaini134 – 1429ExtracellularBy similarity
Transmembranei143 – 16422Helical; Name=M2By similarityAdd
BLAST
Topological domaini165 – 379215CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • astrocyte projection Source: RGD
  • basolateral plasma membrane Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • microvillus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

GuidetoPHARMACOLOGYi438.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379ATP-sensitive inward rectifier potassium channel 10PRO_0000154955Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei338 – 3381PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49655.
PRIDEiP49655.

PTM databases

iPTMnetiP49655.
PhosphoSiteiP49655.

Expressioni

Tissue specificityi

Predominantly expressed in the brain, including in glial cells of the cerebellum and forebrain. Expressed at lower levels in the kidney, and other peripheral tissues.2 Publications

Gene expression databases

GenevisibleiP49655. RN.

Interactioni

Subunit structurei

Heterodimer with Kir5.1/KCNJ16; this interaction is required for KCNJ16 localization to the basolateral membrane in kidney cells. Interacts with MAGI1, alone and possibly as a heterodimer with KCNJ16; this interaction may facilitate KCNJ10/KCNJ16 potassium channel expression at the basolateral membrane in kidney cells (By similarity). Interacts with INADL (By similarity).By similarity

GO - Molecular functioni

  • identical protein binding Source: RGD
  • receptor binding Source: RGD

Protein-protein interaction databases

BioGridi248335. 2 interactions.
IntActiP49655. 3 interactions.
MINTiMINT-103719.
STRINGi10116.ENSRNOP00000010146.

Structurei

3D structure databases

ProteinModelPortaliP49655.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi128 – 1336Selectivity filterBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
HOGENOMiHOG000237326.
HOVERGENiHBG006178.
InParanoidiP49655.
KOiK05003.
OMAiGTWFAFG.
OrthoDBiEOG7GFB4Z.
PhylomeDBiP49655.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003269. K_chnl_inward-rec_Kir1.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF21. PTHR11767:SF21. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01322. KIR12CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P49655-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSVAKVYYS QTTQTESRPL VAPGIRRRRV LTKDGRSNVR MEHIADKRFL
60 70 80 90 100
YLKDLWTTFI DMQWRYKLLL FSATFAGTWF LFGVVWYLVA VAHGDLLELG
110 120 130 140 150
PPANHTPCVV QVHTLTGAFL FSLESQTTIG YGFRYISEEC PLAIVLLIAQ
160 170 180 190 200
LVLTTILEIF ITGTFLAKIA RPKKRAETIR FSQHAVVAYH NGKLCLMIRV
210 220 230 240 250
ANMRKSLLIG CQVTGKLLQT HQTKEGENIR LNQVNVTFQV DTASDSPFLI
260 270 280 290 300
LPLTFYHVVD ETSPLKDLPL RSGEGDFELV LILSGTVEST SATCQVRTSY
310 320 330 340 350
LPEEILWGYE FTPAISLSAS GKYVADFSLF DQVVKVASPG GLRDSTVRYG
360 370
DPEKLKLEES LREQAEKEGS ALSVRISNV
Length:379
Mass (Da):42,480
Last modified:February 1, 1996 - v1
Checksum:i77B3671907B68CB2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731A → P in AAA87811 (PubMed:7624316).Curated
Sequence conflicti76 – 761A → T in AAA87811 (PubMed:7624316).Curated
Sequence conflicti110 – 1123VQV → GAGA (PubMed:7624316).Curated
Sequence conflicti115 – 1162LT → YL (PubMed:7624316).Curated
Sequence conflicti145 – 1451V → E in AAA87811 (PubMed:7624316).Curated
Sequence conflicti190 – 1901H → L in AAA87811 (PubMed:7624316).Curated
Sequence conflicti228 – 2281N → D in AAA87811 (PubMed:7624316).Curated
Sequence conflicti266 – 2661K → Q in AAA87811 (PubMed:7624316).Curated
Sequence conflicti304 – 3041E → R in CAA58568 (PubMed:7874445).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86818 mRNA. Translation: CAA60501.1.
X83585 mRNA. Translation: CAA58568.1.
U27558 mRNA. Translation: AAA87811.1.
PIRiA57477.
RefSeqiNP_113790.2. NM_031602.2.
XP_008767959.1. XM_008769737.1.
UniGeneiRn.10196.

Genome annotation databases

GeneIDi29718.
KEGGirno:29718.
UCSCiRGD:61822. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86818 mRNA. Translation: CAA60501.1.
X83585 mRNA. Translation: CAA58568.1.
U27558 mRNA. Translation: AAA87811.1.
PIRiA57477.
RefSeqiNP_113790.2. NM_031602.2.
XP_008767959.1. XM_008769737.1.
UniGeneiRn.10196.

3D structure databases

ProteinModelPortaliP49655.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248335. 2 interactions.
IntActiP49655. 3 interactions.
MINTiMINT-103719.
STRINGi10116.ENSRNOP00000010146.

Chemistry

GuidetoPHARMACOLOGYi438.

PTM databases

iPTMnetiP49655.
PhosphoSiteiP49655.

Proteomic databases

PaxDbiP49655.
PRIDEiP49655.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29718.
KEGGirno:29718.
UCSCiRGD:61822. rat.

Organism-specific databases

CTDi3766.
RGDi61822. Kcnj10.

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
HOGENOMiHOG000237326.
HOVERGENiHBG006178.
InParanoidiP49655.
KOiK05003.
OMAiGTWFAFG.
OrthoDBiEOG7GFB4Z.
PhylomeDBiP49655.
TreeFamiTF313676.

Miscellaneous databases

PROiP49655.

Gene expression databases

GenevisibleiP49655. RN.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003269. K_chnl_inward-rec_Kir1.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF21. PTHR11767:SF21. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01322. KIR12CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel ATP-dependent inward rectifier potassium channel expressed predominantly in glial cells."
    Takumi T., Ishii T., Horio Y., Morishige K., Takahashi N., Yamada M., Yamashita T., Kiyama H., Sohmiya K., Nakanishi S., Kurachi Y.
    J. Biol. Chem. 270:16339-16346(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Cloning and expression of a family of inward rectifier potassium channels."
    Bond C.T., Pessia M., Xia X.-M., Lagrutta A., Kavanaugh M.P., Adelman J.P.
    Recept. Channels 2:183-191(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "Cloning and expression of two brain-specific inwardly rectifying potassium channels."
    Bredt D.S., Wang T.L., Cohen N.A., Guggino W.B., Snyder S.H.
    Proc. Natl. Acad. Sci. U.S.A. 92:6753-6757(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-338, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKCJ10_RAT
AccessioniPrimary (citable) accession number: P49655
Secondary accession number(s): Q62790
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.