Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

P2X purinoceptor 2

Gene

P2rx2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ion channel gated by extracellular ATP involved in a variety of cellular responses, such as excitatory postsynaptic responses in sensory neurons, neuromuscular junctions (NMJ) formation, hearing, perception of taste and peristalsis. In the inner ear, regulates sound transduction and auditory neurotransmission, outer hair cell electromotility, inner ear gap junctions, and K+ recycling. Mediates synaptic transmission between neurons and from neurons to smooth muscle (By similarity).By similarity

GO - Molecular functioni

  • ATP binding Source: RGD
  • cadmium ion binding Source: RGD
  • cobalt ion binding Source: RGD
  • copper ion binding Source: RGD
  • drug binding Source: RGD
  • extracellular ATP-gated cation channel activity Source: RGD
  • identical protein binding Source: BHF-UCL
  • ligand-gated ion channel activity Source: RGD
  • mercury ion binding Source: RGD
  • nickel cation binding Source: RGD
  • phosphatidylinositol binding Source: RGD
  • purinergic nucleotide receptor activity Source: InterPro
  • zinc ion binding Source: RGD

GO - Biological processi

  • neuronal action potential Source: RGD
  • protein heterooligomerization Source: RGD
  • protein homooligomerization Source: RGD
  • response to ATP Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Protein family/group databases

TCDBi1.A.7.1.2. the atp-gated p2x receptor cation channel (p2x receptor) family.

Names & Taxonomyi

Protein namesi
Recommended name:
P2X purinoceptor 2
Short name:
P2X2
Alternative name(s):
ATP receptor
Purinergic receptor
Gene namesi
Name:P2rx2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi620251. P2rx2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3434CytoplasmicSequence analysisAdd
BLAST
Transmembranei35 – 5218Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini53 – 326274ExtracellularSequence analysisAdd
BLAST
Transmembranei327 – 34721Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini348 – 472125CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell surface Source: RGD
  • dendritic spine Source: RGD
  • integral component of membrane Source: RGD
  • integral component of plasma membrane Source: InterPro
  • membrane Source: RGD
  • neuronal cell body Source: RGD
  • plasma membrane Source: BHF-UCL
  • postsynaptic density Source: RGD
  • presynaptic membrane Source: RGD
  • terminal bouton Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3137277.
GuidetoPHARMACOLOGYi479.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472P2X purinoceptor 2PRO_0000161550Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi9 ↔ 4301 Publication
Disulfide bondi113 ↔ 164By similarity
Disulfide bondi124 ↔ 147By similarity
Disulfide bondi130 ↔ 158By similarity
Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence analysis
Disulfide bondi214 ↔ 224By similarity
Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence analysis
Disulfide bondi258 ↔ 267By similarity
Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP49653.

PTM databases

PhosphoSiteiP49653.

Expressioni

Tissue specificityi

High levels in pituitary and vas deferens. Lower extent in spinal cord, bladder, brain, adrenal, testis, sensory epithelia from the inner ear.

Gene expression databases

ExpressionAtlasiP49653. baseline and differential.
GenevisibleiP49653. RN.

Interactioni

Subunit structurei

Homotrimer and heterotrimer; functional P2XRs are organized as homomeric and heteromeric trimers.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
P2rx4P515774EBI-9511543,EBI-9511617
P2rx6P515794EBI-9511543,EBI-9511515

GO - Molecular functioni

  • identical protein binding Source: BHF-UCL

Protein-protein interaction databases

DIPiDIP-48329N.
IntActiP49653. 2 interactions.
STRINGi10116.ENSRNOP00000053533.

Chemistry

BindingDBiP49653.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni309 – 32214Pore-forming motifSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi300 – 3056Poly-Thr
Compositional biasi392 – 3954Poly-Pro

Sequence similaritiesi

Belongs to the P2X receptor family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGJK. Eukaryota.
ENOG410ZQ3M. LUCA.
GeneTreeiENSGT00390000016028.
HOVERGENiHBG053086.
InParanoidiP49653.
KOiK05216.
OMAiLYFVWYV.
OrthoDBiEOG78PV92.
PhylomeDBiP49653.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003045. P2X2_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF4. PTHR10125:SF4. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PIRSFiPIRSF005713. P2X_purinoceptor. 1 hit.
PRINTSiPR01309. P2X2RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform P2RX2-1 (identifier: P49653-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVRRLARGCW SAFWDYETPK VIVVRNRRLG FVHRMVQLLI LLYFVWYVFI
60 70 80 90 100
VQKSYQDSET GPESSIITKV KGITMSEDKV WDVEEYVKPP EGGSVVSIIT
110 120 130 140 150
RIEVTPSQTL GTCPESMRVH SSTCHSDDDC IAGQLDMQGN GIRTGHCVPY
160 170 180 190 200
YHGDSKTCEV SAWCPVEDGT SDNHFLGKMA PNFTILIKNS IHYPKFKFSK
210 220 230 240 250
GNIASQKSDY LKHCTFDQDS DPYCPIFRLG FIVEKAGENF TELAHKGGVI
260 270 280 290 300
GVIINWNCDL DLSESECNPK YSFRRLDPKY DPASSGYNFR FAKYYKINGT
310 320 330 340 350
TTTRTLIKAY GIRIDVIVHG QAGKFSLIPT IINLATALTS IGVGSFLCDW
360 370 380 390 400
ILLTFMNKNK LYSHKKFDKV RTPKHPSSRW PVTLALVLGQ IPPPPSHYSQ
410 420 430 440 450
DQPPSPPSGE GPTLGEGAEL PLAVQSPRPC SISALTEQVV DTLGQHMGQR
460 470
PPVPEPSQQD STSTDPKGLA QL
Length:472
Mass (Da):52,617
Last modified:February 1, 1996 - v1
Checksum:iAB53E09A2CADA6A2
GO
Isoform P2RX2-2 (identifier: P49653-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     344-360: GSFLCDWILLTFMNKNK → VRNPNSLGDLGQVGSVW
     361-472: Missing.

Show »
Length:360
Mass (Da):40,415
Checksum:iA4E21732CB9ADB4D
GO
Isoform P2RX2-3 (identifier: P49653-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     173-199: Missing.

Show »
Length:445
Mass (Da):49,441
Checksum:iCB6FE2541A57969B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei173 – 19927Missing in isoform P2RX2-3. CuratedVSP_004500Add
BLAST
Alternative sequencei344 – 36017GSFLC…MNKNK → VRNPNSLGDLGQVGSVW in isoform P2RX2-2. CuratedVSP_004501Add
BLAST
Alternative sequencei361 – 472112Missing in isoform P2RX2-2. CuratedVSP_004502Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14414 mRNA. Translation: AAA50756.1.
Y09910 Genomic DNA. Translation: CAA71046.1.
L43511 mRNA. Translation: AAC42067.1.
AF020756 mRNA. Translation: AAB94570.1.
PIRiS50859.
RefSeqiNP_446108.2. NM_053656.2. [P49653-1]
UniGeneiRn.88799.

Genome annotation databases

EnsembliENSRNOT00000056693; ENSRNOP00000053532; ENSRNOG00000037456. [P49653-3]
GeneIDi114115.
KEGGirno:114115.
UCSCiRGD:620251. rat. [P49653-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14414 mRNA. Translation: AAA50756.1.
Y09910 Genomic DNA. Translation: CAA71046.1.
L43511 mRNA. Translation: AAC42067.1.
AF020756 mRNA. Translation: AAB94570.1.
PIRiS50859.
RefSeqiNP_446108.2. NM_053656.2. [P49653-1]
UniGeneiRn.88799.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48329N.
IntActiP49653. 2 interactions.
STRINGi10116.ENSRNOP00000053533.

Chemistry

BindingDBiP49653.
ChEMBLiCHEMBL3137277.
GuidetoPHARMACOLOGYi479.

Protein family/group databases

TCDBi1.A.7.1.2. the atp-gated p2x receptor cation channel (p2x receptor) family.

PTM databases

PhosphoSiteiP49653.

Proteomic databases

PaxDbiP49653.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000056693; ENSRNOP00000053532; ENSRNOG00000037456. [P49653-3]
GeneIDi114115.
KEGGirno:114115.
UCSCiRGD:620251. rat. [P49653-1]

Organism-specific databases

CTDi22953.
RGDi620251. P2rx2.

Phylogenomic databases

eggNOGiENOG410IGJK. Eukaryota.
ENOG410ZQ3M. LUCA.
GeneTreeiENSGT00390000016028.
HOVERGENiHBG053086.
InParanoidiP49653.
KOiK05216.
OMAiLYFVWYV.
OrthoDBiEOG78PV92.
PhylomeDBiP49653.

Miscellaneous databases

NextBioi618313.
PROiP49653.

Gene expression databases

ExpressionAtlasiP49653. baseline and differential.
GenevisibleiP49653. RN.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003045. P2X2_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF4. PTHR10125:SF4. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PIRSFiPIRSF005713. P2X_purinoceptor. 1 hit.
PRINTSiPR01309. P2X2RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "New structural motif for ligand-gated ion channels defined by an ionotropic ATP receptor."
    Brake A.J., Wagenbach M.J., Julius D.
    Nature 371:519-523(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Wistar.
    Tissue: Liver.
  3. "Identification of a short form of the P2xR1-purinoceptor subunit produced by alternative splicing in the pituitary and cochlea."
    Housley G.D., Greenwood D., Bennett T., Ryan A.F.
    Biochem. Biophys. Res. Commun. 212:501-508(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 326-340, ALTERNATIVE SPLICING.
    Strain: Sprague-Dawley and Wistar.
    Tissue: Vas deferens.
  4. Koshimizu T., Tomic M., van Goor F., Stojilkovic S.S.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM P2RX2-3).
    Strain: Sprague-Dawley.
    Tissue: Pituitary.
  5. "Intracellular disulfide bond that affects ATP responsiveness of P2X(2) receptor/channel."
    Nakazawa K., Ojima H., Ishii-Nozawa R., Takeuchi K., Ohno Y.
    Eur. J. Pharmacol. 474:205-208(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BOND.

Entry informationi

Entry nameiP2RX2_RAT
AccessioniPrimary (citable) accession number: P49653
Secondary accession number(s): O54868
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 11, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.