Reviewed, UniProtKB/Swiss-Prot P49644 (G3PC_CHLRE)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
    EC=1.2.1.12

Gene names

Name:

GAPC

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Miscellaneous	Algae contain three forms of GAPDH: two cytosolic forms which participate in glycolysis and one chloroplastic form which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Glyceraldehyde-3-phosphate dehydrogenase, cytosolic		PRO_0000145596
Regions
	Nucleotide binding	14 – 15	2	NAD By similarity
	Region	153 – 155	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	213 – 214	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	154	1	Nucleophile By similarity
	Binding site	36	1	NAD By similarity
	Binding site	184	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	236	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	318	1	NAD By similarity
	Site	181	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49644-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: A081514C3B13E2F7	FASTA	341	36,559
	10 20 30 40 50 60 MSGKIKIGIN GFGRIGRLVM RATMLRPDIE VVAINDPFID AEYMAYMFKY DSVHKTWPGH 70 80 90 100 110 120 VNGSKDGFLV EGRKIHTFTE SDPSKINWGA AGADIVIEST GVFTDIAKAT AHLTGGAKKV 130 140 150 160 170 180 IITAPSNDAP MYVMGVNHEK YNPATDHIIS NASCTTNCLA PLAKVVNSKF GIKEGLMTTV 190 200 210 220 230 240 HATTATQKTV DGPSKKDWRG GRAVNGNIIP SSTGAAKAVG KVLPELKGKL TGMAFRVPTN 250 260 270 280 290 300 DVSVVDLTVT LEKATTYEDI MKALKEASEG EMKGVLAYTD EDVVSSDFVT DPASCTVDAK 310 320 330 340 AGIMLSPTFV KLVAWYDNEW GYSNRVVDLA LHVAKKAAVK V

References

[1]	"Five identical intron positions in ancient duplicated genes of eubacterial origin." Kersanach R., Brinkmann H., Liaud M.-F., Zhang D.-X., Martin W., Cerff R. Nature 367:387-389(1994) [PubMed: 8114942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1132.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L27669 Genomic DNA. Translation: AAA86856.1.
PIR	T08147.
3D structure databases
HSSP	HSSP built from PDB template 1DC5 based on UniProtKB P06977.
ModBase	Search...
Proteomic databases
PRIDE	P49644.
Enzyme and pathway databases
BRENDA	1.2.1.12. 144.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PC_CHLRE

Accession

Primary (citable) accession number: P49644

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)