DNA primase small subunit - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot P49642 (PRI1_HUMAN)

Last modified October 13, 2009. Version 84. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    DNA primase small subunit
    EC=2.7.7.-
Alternative name(s):
    DNA primase 49 kDa subunit
      Short name=p49

Gene names

Name:

PRIM1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.
Subunit structure	Heterodimer of a small subunit and a large subunit.
Miscellaneous	The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA By similarity.
Sequence similarities	Belongs to the eukaryotic-type primase small subunit family.

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Ontologies

Keywords
Biological process	DNA replication Transcription
Cellular component	DNA-directed RNA polymerase Primosome
Coding sequence diversity	Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Ref.1 Traceable author statement. Source: ProtInc transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW nucleoplasm Inferred from Experiment. Source: Reactome
Molecular function	DNA primase activity Ref.1 Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 420

420

DNA primase small subunit

PRO_0000046730

Regions

Zinc finger

121 – 131

Knuckle-type By similarity

Sites

Active site

Potential

Active site

109

Potential

Active site

111

Potential

Natural variations

Natural variant

D → A: dbSNP rs2277339.

VAR_021898

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Sequences

Sequence

Length

Mass (Da)

Tools

P49642-1 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 9B5AC900E0C3CCE8
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FASTA

420

49,902

        10         20         30         40         50         60 
METFDPTELP ELLKLYYRRL FPYSQYYRWL NYGGVIKNYF QHREFSFTLK DDIYIRYQSF 

        70         80         90        100        110        120 
NNQSDLEKEM QKMNPYKIDI GAVYSHRPNQ HNTVKLGAFQ AQEKELVFDI DMTDYDDVRR 

       130        140        150        160        170        180 
CCSSADICPK CWTLMTMAIR IIDRALKEDF GFKHRLWVYS GRRGVHCWVC DESVRKLSSA 

       190        200        210        220        230        240 
VRSGIVEYLS LVKGGQDVKK KVHLSEKIHP FIRKSINIIK KYFEEYALVN QDILENKESW 

       250        260        270        280        290        300 
DKILALVPET IHDELQQSFQ KSHNSLQRWE HLKKVASRYQ NNIKNDKYGP WLEWEIMLQY 

       310        320        330        340        350        360 
CFPRLDINVS KGINHLLKSP FSVHPKTGRI SVPIDLQKVD QFDPFTVPTI SFICRELDAI 

       370        380        390        400        410        420 
STNEEEKEEN EAESDVKHRT RDYKKTSLAP YVKVFEHFLE NLDKSRKGEL LKKSDLQKDF

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA replication in vitro by recombinant DNA-polymerase-alpha-primase." Stadlbauer F., Brueckner A., Rehfuess C., Eckerskorn C., Lottspeich F., Foerster V., Tseng B.Y., Nasheuer H.-P. Eur. J. Biochem. 222:781-793(1994) [PubMed: 8026492] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[3]	"Mapping of the human DNA primase 1 (PRIM1) to chromosome 12q13." Cloutier S., Hamel H., Champagne M., Yotov W.V. Genomics 43:398-401(1997) [PubMed: 9268648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 97-146.
[4]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

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Cross-references

Sequence databases
	X74330 mRNA. Translation: CAA52377.1. BC005266 mRNA. Translation: AAH05266.1. U89689 Genomic DNA. Translation: AAC51726.1.
IPI	IPI00027704.
PIR	S45630.
RefSeq	NP_000937.1.
UniGene	Hs.534339
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P49642. 3 interactions.
STRING	P49642.
PTM databases
PhosphoSite	P49642.
Proteomic databases
PRIDE	P49642.
Genome annotation databases
Ensembl	ENST00000338193; ENSP00000350491; ENSG00000198056; Homo sapiens. [Genome view]
GeneID	5557.
KEGG	hsa:5557.
UCSC	uc001smd.1. human.
Organism-specific databases
CTD	5557.
GeneCards	GC12M055411.
H-InvDB	HIX0010739.
HGNC	HGNC:9369. PRIM1.
MIM	176635. gene.
PharmGKB	PA33739.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P49642.
HOVERGEN	P49642.
Enzyme and pathway databases
Reactome	REACT_152. Cell Cycle, Mitotic. REACT_383. DNA Replication. REACT_7970. Telomere Maintenance.
Gene expression databases
ArrayExpress	P49642.
Bgee	P49642.
CleanEx	HS_PRIM1.
Genevestigator	P49642.
GermOnline	ENSG00000198056. Homo sapiens.
Family and domain databases
InterPro	IPR002755. DNA_primase_S. IPR014052. DNA_primase_ssu_euk/arc. [Graphical view]
PANTHER	PTHR10536. DNA_primase_S_euk_arch. 1 hit.
Pfam	PF01896. DNA_primase_S. 1 hit. [Graphical view]
TIGRFAMs	TIGR00335. primase_sml. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	21534.
SOURCE	Search...

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Entry information

Entry name

PRI1_HUMAN

Accession

Primary (citable) accession number: P49642

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	October 13, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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