P49642 (PRI1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA primase small subunit
EC=2.7.7.-

Alternative name(s):
DNA primase 49 kDa subunit
Short name=p49

Gene names

Name:

PRIM1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.
Subunit structure	Heterodimer of a small subunit and a large subunit.
Miscellaneous	The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA By similarity.
Sequence similarities	Belongs to the eukaryotic-type primase small subunit family.

Ontologies

Keywords
Biological process	DNA replication Transcription
Cellular component	DNA-directed RNA polymerase Primosome
Coding sequence diversity	Polymorphism
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Traceable author statement. Source: ProtInc DNA strand elongation involved in DNA replication Traceable author statement. Source: Reactome DNA-dependent DNA replication initiation Traceable author statement. Source: Reactome G1/S transition of mitotic cell cycle Traceable author statement. Source: Reactome M/G1 transition of mitotic cell cycle Traceable author statement. Source: Reactome S phase of mitotic cell cycle Traceable author statement. Source: Reactome telomere maintenance via recombination Traceable author statement. Source: Reactome telomere maintenance via semi-conservative replication Traceable author statement. Source: Reactome
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW nucleoplasm Traceable author statement. Source: Reactome
Molecular function	DNA primase activity Traceable author statement. Source: ProtInc metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	DNA primase small subunit		PRO_0000046730
Regions
	Motif	121 – 131	11	Zinc knuckle motif
Sites
	Active site	44	1	Potential
	Active site	109	1	Potential
	Active site	111	1	Potential
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.4
Natural variations
	Natural variant	5	1	D → A. Corresponds to variant rs2277339 [ dbSNP | Ensembl ].		VAR_021898

Sequences

	Sequence		Length	Mass (Da)
	P49642 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 9B5AC900E0C3CCE8	FASTA	420	49,902
	10 20 30 40 50 60 METFDPTELP ELLKLYYRRL FPYSQYYRWL NYGGVIKNYF QHREFSFTLK DDIYIRYQSF 70 80 90 100 110 120 NNQSDLEKEM QKMNPYKIDI GAVYSHRPNQ HNTVKLGAFQ AQEKELVFDI DMTDYDDVRR 130 140 150 160 170 180 CCSSADICPK CWTLMTMAIR IIDRALKEDF GFKHRLWVYS GRRGVHCWVC DESVRKLSSA 190 200 210 220 230 240 VRSGIVEYLS LVKGGQDVKK KVHLSEKIHP FIRKSINIIK KYFEEYALVN QDILENKESW 250 260 270 280 290 300 DKILALVPET IHDELQQSFQ KSHNSLQRWE HLKKVASRYQ NNIKNDKYGP WLEWEIMLQY 310 320 330 340 350 360 CFPRLDINVS KGINHLLKSP FSVHPKTGRI SVPIDLQKVD QFDPFTVPTI SFICRELDAI 370 380 390 400 410 420 STNEEEKEEN EAESDVKHRT RDYKKTSLAP YVKVFEHFLE NLDKSRKGEL LKKSDLQKDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA replication in vitro by recombinant DNA-polymerase-alpha-primase." Stadlbauer F., Brueckner A., Rehfuess C., Eckerskorn C., Lottspeich F., Foerster V., Tseng B.Y., Nasheuer H.-P. Eur. J. Biochem. 222:781-793(1994) [PubMed: 8026492] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[3]	"Mapping of the human DNA primase 1 (PRIM1) to chromosome 12q13." Cloutier S., Hamel H., Champagne M., Yotov W.V. Genomics 43:398-401(1997) [PubMed: 9268648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 97-146.
[4]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74330 mRNA. Translation: CAA52377.1. BC005266 mRNA. Translation: AAH05266.1. U89689 Genomic DNA. Translation: AAC51726.1.
IPI	IPI00027704.
PIR	S45630.
RefSeq	NP_000937.1. NM_000946.2.
UniGene	Hs.534339.
3D structure databases
ProteinModelPortal	P49642.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49642. 4 interactions.
MINT	MINT-234425.
STRING	P49642.
PTM databases
PhosphoSite	P49642.
Polymorphism databases
DMDM	1346792.
Proteomic databases
PRIDE	P49642.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338193; ENSP00000350491; ENSG00000198056.
GeneID	5557.
KEGG	hsa:5557.
UCSC	uc001smd.1. human.
Organism-specific databases
CTD	5557.
GeneCards	GC12M057097.
H-InvDB	HIX0201875.
HGNC	HGNC:9369. PRIM1.
HPA	HPA040010.
MIM	176635. gene.
neXtProt	NX_P49642.
PharmGKB	PA33739.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07252.
HOGENOM	HBG397483.
HOVERGEN	HBG003006.
InParanoid	P49642.
OMA	EDFGFEH.
OrthoDB	EOG4WQ12H.
PhylomeDB	P49642.
Enzyme and pathway databases
Reactome	REACT_152. Cell Cycle, Mitotic. REACT_22172. Chromosome Maintenance. REACT_383. DNA Replication.
Gene expression databases
ArrayExpress	P49642.
Bgee	P49642.
CleanEx	HS_PRIM1.
Genevestigator	P49642.
GermOnline	ENSG00000198056. Homo sapiens.
Family and domain databases
InterPro	IPR002755. DNA_primase_S. IPR014052. DNA_primase_ssu_euk/arc. [Graphical view]
KO	K02684.
PANTHER	PTHR10536. DNA_primase_S_euk_arch. 1 hit.
Pfam	PF01896. DNA_primase_S. 1 hit. [Graphical view]
TIGRFAMs	TIGR00335. Primase_sml. 1 hit.
ProtoNet	Search...
Other
NextBio	21534.
SOURCE	Search...

Entry information

Entry name

PRI1_HUMAN

Accession

Primary (citable) accession number: P49642

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.