Reviewed, UniProtKB/Swiss-Prot P49621 (DGKB_RAT)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase beta
      Short name=DAG kinase beta
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase beta
      Short name=DGK-beta
    90 kDa diacylglycerol kinase

Gene names

Name:	Dgkb
Synonyms:	Dagk2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	801 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Exhibits high phosphorylation activity for long-chain diacylglycerols.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Enzyme regulation	Stimulated by phosphatidylserine.
Subcellular location	Cytoplasm. Membrane. Note: Can be loosely bound to the membranes.
Tissue specificity	Confined to restricted neuronal populations such as the caudate-putamen, the accumbens nucleus, and the olfactory tubercle.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 EF-hand domains. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Ref.1 Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Ref.1 Inferred from direct assay. Source: RGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 801	801	Diacylglycerol kinase beta		PRO_0000218458
Regions
	Domain	148 – 183	36	EF-hand 1
	Domain	193 – 228	36	EF-hand 2
	Domain	431 – 565	135	DAGKc
	Calcium binding	161 – 172	12	1 Potential
	Calcium binding	206 – 217	12	2 Potential
	Zinc finger	243 – 292	50	Phorbol-ester/DAG-type 1
	Zinc finger	307 – 356	50	Phorbol-ester/DAG-type 2

Sequences

	Sequence		Length	Mass (Da)
	P49621-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: F30874CD2DCE363D	FASTA	801	90,288
	10 20 30 40 50 60 MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK QDILNQTIDF 70 80 90 100 110 120 EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPNVKS KPALLSGGLR MNKGAITPPR 130 140 150 160 170 180 SSPANTCFPE VIHLKDIVCY LSLLERGRPE DKLEFMFRLY DTDGNGFLDS SELENIIGQM 190 200 210 220 230 240 MHVAEYLEWD VTELNPILHE MMEEIDYDRD GTVSLEEWIQ GGMTTIPLLV LLGLENNVKD 250 260 270 280 290 300 DGQHVWRLKH FNKPAYCNLC LNMLIGVGKQ GLCCSFCKYT VHERCARAPP SCIKTYVKSK 310 320 330 340 350 360 KNTDVMHHYW VEGNCPTKCD KCHKTVKCYQ GLTGLHCVWC QTTLHNKCAS HLKPECDCGP 370 380 390 400 410 420 LKDHILPPTT ICPVVLTMPT AGTSVPEERQ STAKKEKGSS QQPNKVTDKN KMQRANSVTM 430 440 450 460 470 480 DGQGLQITPI PGTHPLLVFV NPKSGGKQGE RIYRKFQYLL NPRQVYSLSG NGPMPGLHFF 490 500 510 520 530 540 RDVPDFRVLA CGGDGTVGWI LDCIEKANVV KHPPVAILPL GTGNDLARCL RWGGGYEGEN 550 560 570 580 590 600 LMKILKDIES STEIMLDRWK FEVTPNDKDE KGDPVPYSII NNYFSIGVDA SIAHRFHIMR 610 620 630 640 650 660 EKHPEKFNSR MKNKFWYFEF GTSETFSATC KKLHESVEIE CDGVQIDLIN ISLQGIAILN 670 680 690 700 710 720 IPSMHGGSNL WGESKKKRSH RRIEKKGSDK RPTLTDAKEL KFASQDLSDQ LLEVVGLEGA 730 740 750 760 770 780 MEMGQIYTGL KSAGRRLAQC SSVVIRTSKS LPMQIDGEPW MQTPCTIKIT HKNQAPMLMG 790 800 PPPKTGLFCS LIKRTRNRSK E

References

[1]	"Molecular cloning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons." Goto K., Kondo H. Proc. Natl. Acad. Sci. U.S.A. 90:7598-7602(1993) [PubMed: 7689223] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D16100 mRNA. Translation: BAA03675.1.
IPI	IPI00196195.
PIR	A47744.
RefSeq	NP_062177.1.
UniGene	Rn.11413
3D structure databases
HSSP	HSSP built from PDB template 1TBN based on UniProtKB P05697.
ModBase	Search...
Protein-protein interaction databases
STRING	P49621.
PTM databases
PhosphoSite	P49621.
Proteomic databases
PRIDE	P49621.
Genome annotation databases
Ensembl	ENSRNOT00000037145; ENSRNOP00000034610; ENSRNOG00000030771; Rattus norvegicus. [Genome view]
GeneID	54248.
KEGG	rno:54248.
Organism-specific databases
CTD	54248.
RGD	2488. Dgkb.
Phylogenomic databases
HOVERGEN	P49621.
Enzyme and pathway databases
BRENDA	2.7.1.107. 248.
Gene expression databases
ArrayExpress	P49621.
Genevestigator	P49621.
GermOnline	ENSRNOG00000030771. Rattus norvegicus.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kinase_access. IPR001206. Diacylglycerol_kinase_cat. IPR018248. EF_hand. IPR018247. EF_HAND_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. [Graphical view]
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00036. efhand. 2 hits. [Graphical view]
ProDom	PD002939. DAGKa. 1 hit. PD005043. DAGKc. 1 hit. PD000012. EF-hand. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	610736.

Entry information

Entry name

DGKB_RAT

Accession

Primary (citable) accession number: P49621

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)