Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Diacylglycerol kinase beta

Gene

Dgkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exhibits high phosphorylation activity for long-chain diacylglycerols.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by phosphatidylserine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi161 – 1721PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi206 – 2172PROSITE-ProRule annotationAdd BLAST12
Zinc fingeri243 – 292Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri307 – 356Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium ion binding Source: InterPro
  • diacylglycerol kinase activity Source: RGD
  • NAD+ kinase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.107 5301

Chemistry databases

SwissLipidsiSLP:000000927

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase beta (EC:2.7.1.107)
Short name:
DAG kinase beta
Alternative name(s):
90 kDa diacylglycerol kinase
Diglyceride kinase beta
Short name:
DGK-beta
Gene namesi
Name:Dgkb
Synonyms:Dagk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2488 Dgkb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002184581 – 801Diacylglycerol kinase betaAdd BLAST801

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei117PhosphothreonineCombined sources1
Modified residuei417PhosphoserineCombined sources1
Modified residuei790PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49621
PRIDEiP49621

PTM databases

iPTMnetiP49621
PhosphoSitePlusiP49621

Expressioni

Tissue specificityi

Confined to restricted neuronal populations such as the caudate-putamen, the accumbens nucleus, and the olfactory tubercle.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058842

Structurei

3D structure databases

ProteinModelPortaliP49621
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 183EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini193 – 228EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini431 – 565DAGKcPROSITE-ProRule annotationAdd BLAST135

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri243 – 292Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri307 – 356Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
HOGENOMiHOG000252931
HOVERGENiHBG051345
InParanoidiP49621
KOiK00901
PhylomeDBiP49621

Family and domain databases

CDDicd00029 C1, 1 hit
cd00051 EFh, 1 hit
Gene3Di1.10.238.110, 2 hits
3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR029477 DAG_kinase_typeI_N
IPR037607 DGK
IPR038199 DGK_typeI_N_sf
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF14513 DAG_kinase_N, 2 hits
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF111331 SSF111331, 2 hits
SSF47473 SSF47473, 3 hits
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequencei

Sequence statusi: Complete.

P49621-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK
60 70 80 90 100
QDILNQTIDF EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPNVKS
110 120 130 140 150
KPALLSGGLR MNKGAITPPR SSPANTCFPE VIHLKDIVCY LSLLERGRPE
160 170 180 190 200
DKLEFMFRLY DTDGNGFLDS SELENIIGQM MHVAEYLEWD VTELNPILHE
210 220 230 240 250
MMEEIDYDRD GTVSLEEWIQ GGMTTIPLLV LLGLENNVKD DGQHVWRLKH
260 270 280 290 300
FNKPAYCNLC LNMLIGVGKQ GLCCSFCKYT VHERCARAPP SCIKTYVKSK
310 320 330 340 350
KNTDVMHHYW VEGNCPTKCD KCHKTVKCYQ GLTGLHCVWC QTTLHNKCAS
360 370 380 390 400
HLKPECDCGP LKDHILPPTT ICPVVLTMPT AGTSVPEERQ STAKKEKGSS
410 420 430 440 450
QQPNKVTDKN KMQRANSVTM DGQGLQITPI PGTHPLLVFV NPKSGGKQGE
460 470 480 490 500
RIYRKFQYLL NPRQVYSLSG NGPMPGLHFF RDVPDFRVLA CGGDGTVGWI
510 520 530 540 550
LDCIEKANVV KHPPVAILPL GTGNDLARCL RWGGGYEGEN LMKILKDIES
560 570 580 590 600
STEIMLDRWK FEVTPNDKDE KGDPVPYSII NNYFSIGVDA SIAHRFHIMR
610 620 630 640 650
EKHPEKFNSR MKNKFWYFEF GTSETFSATC KKLHESVEIE CDGVQIDLIN
660 670 680 690 700
ISLQGIAILN IPSMHGGSNL WGESKKKRSH RRIEKKGSDK RPTLTDAKEL
710 720 730 740 750
KFASQDLSDQ LLEVVGLEGA MEMGQIYTGL KSAGRRLAQC SSVVIRTSKS
760 770 780 790 800
LPMQIDGEPW MQTPCTIKIT HKNQAPMLMG PPPKTGLFCS LIKRTRNRSK

E
Length:801
Mass (Da):90,288
Last modified:February 1, 1996 - v1
Checksum:iF30874CD2DCE363D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16100 mRNA Translation: BAA03675.1
PIRiA47744
RefSeqiNP_062177.1, NM_019304.1
UniGeneiRn.11413

Genome annotation databases

GeneIDi54248
KEGGirno:54248
UCSCiRGD:2488 rat

Similar proteinsi

Entry informationi

Entry nameiDGKB_RAT
AccessioniPrimary (citable) accession number: P49621
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health