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P49621

- DGKB_RAT

UniProt

P49621 - DGKB_RAT

Protein

Diacylglycerol kinase beta

Gene

Dgkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Exhibits high phosphorylation activity for long-chain diacylglycerols.

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Enzyme regulationi

    Stimulated by phosphatidylserine.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi161 – 172121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi206 – 217122PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri243 – 29250Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri307 – 35650Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. diacylglycerol kinase activity Source: RGD
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase beta (EC:2.7.1.107)
    Short name:
    DAG kinase beta
    Alternative name(s):
    90 kDa diacylglycerol kinase
    Diglyceride kinase beta
    Short name:
    DGK-beta
    Gene namesi
    Name:Dgkb
    Synonyms:Dagk2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2488. Dgkb.

    Subcellular locationi

    Cytoplasm. Membrane
    Note: Can be loosely bound to the membranes.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 801801Diacylglycerol kinase betaPRO_0000218458Add
    BLAST

    Proteomic databases

    PRIDEiP49621.

    PTM databases

    PhosphoSiteiP49621.

    Expressioni

    Tissue specificityi

    Confined to restricted neuronal populations such as the caudate-putamen, the accumbens nucleus, and the olfactory tubercle.

    Gene expression databases

    GenevestigatoriP49621.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000058842.

    Structurei

    3D structure databases

    ProteinModelPortaliP49621.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini148 – 18336EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini193 – 22836EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini431 – 565135DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri243 – 29250Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri307 – 35650Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    HOGENOMiHOG000252931.
    HOVERGENiHBG051345.
    InParanoidiP49621.
    KOiK00901.
    PhylomeDBiP49621.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    1.10.238.110. 1 hit.
    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P49621-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK    50
    QDILNQTIDF EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPNVKS 100
    KPALLSGGLR MNKGAITPPR SSPANTCFPE VIHLKDIVCY LSLLERGRPE 150
    DKLEFMFRLY DTDGNGFLDS SELENIIGQM MHVAEYLEWD VTELNPILHE 200
    MMEEIDYDRD GTVSLEEWIQ GGMTTIPLLV LLGLENNVKD DGQHVWRLKH 250
    FNKPAYCNLC LNMLIGVGKQ GLCCSFCKYT VHERCARAPP SCIKTYVKSK 300
    KNTDVMHHYW VEGNCPTKCD KCHKTVKCYQ GLTGLHCVWC QTTLHNKCAS 350
    HLKPECDCGP LKDHILPPTT ICPVVLTMPT AGTSVPEERQ STAKKEKGSS 400
    QQPNKVTDKN KMQRANSVTM DGQGLQITPI PGTHPLLVFV NPKSGGKQGE 450
    RIYRKFQYLL NPRQVYSLSG NGPMPGLHFF RDVPDFRVLA CGGDGTVGWI 500
    LDCIEKANVV KHPPVAILPL GTGNDLARCL RWGGGYEGEN LMKILKDIES 550
    STEIMLDRWK FEVTPNDKDE KGDPVPYSII NNYFSIGVDA SIAHRFHIMR 600
    EKHPEKFNSR MKNKFWYFEF GTSETFSATC KKLHESVEIE CDGVQIDLIN 650
    ISLQGIAILN IPSMHGGSNL WGESKKKRSH RRIEKKGSDK RPTLTDAKEL 700
    KFASQDLSDQ LLEVVGLEGA MEMGQIYTGL KSAGRRLAQC SSVVIRTSKS 750
    LPMQIDGEPW MQTPCTIKIT HKNQAPMLMG PPPKTGLFCS LIKRTRNRSK 800
    E 801
    Length:801
    Mass (Da):90,288
    Last modified:February 1, 1996 - v1
    Checksum:iF30874CD2DCE363D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16100 mRNA. Translation: BAA03675.1.
    PIRiA47744.
    RefSeqiNP_062177.1. NM_019304.1.
    UniGeneiRn.11413.

    Genome annotation databases

    GeneIDi54248.
    KEGGirno:54248.
    UCSCiRGD:2488. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16100 mRNA. Translation: BAA03675.1 .
    PIRi A47744.
    RefSeqi NP_062177.1. NM_019304.1.
    UniGenei Rn.11413.

    3D structure databases

    ProteinModelPortali P49621.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000058842.

    PTM databases

    PhosphoSitei P49621.

    Proteomic databases

    PRIDEi P49621.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 54248.
    KEGGi rno:54248.
    UCSCi RGD:2488. rat.

    Organism-specific databases

    CTDi 1607.
    RGDi 2488. Dgkb.

    Phylogenomic databases

    eggNOGi NOG47311.
    HOGENOMi HOG000252931.
    HOVERGENi HBG051345.
    InParanoidi P49621.
    KOi K00901.
    PhylomeDBi P49621.

    Miscellaneous databases

    NextBioi 610736.

    Gene expression databases

    Genevestigatori P49621.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    1.10.238.110. 1 hit.
    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons."
      Goto K., Kondo H.
      Proc. Natl. Acad. Sci. U.S.A. 90:7598-7602(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain.

    Entry informationi

    Entry nameiDGKB_RAT
    AccessioniPrimary (citable) accession number: P49621
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3