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P49621

- DGKB_RAT

UniProt

P49621 - DGKB_RAT

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Protein
Diacylglycerol kinase beta
Gene
Dgkb, Dagk2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Exhibits high phosphorylation activity for long-chain diacylglycerols.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by phosphatidylserine.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi161 – 172121 Reviewed prediction
Add
BLAST
Calcium bindingi206 – 217122 Reviewed prediction
Add
BLAST
Zinc fingeri243 – 29250Phorbol-ester/DAG-type 1
Add
BLAST
Zinc fingeri307 – 35650Phorbol-ester/DAG-type 2
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: InterPro
  3. calcium ion binding Source: InterPro
  4. diacylglycerol kinase activity Source: RGD

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase beta (EC:2.7.1.107)
Short name:
DAG kinase beta
Alternative name(s):
90 kDa diacylglycerol kinase
Diglyceride kinase beta
Short name:
DGK-beta
Gene namesi
Name:Dgkb
Synonyms:Dagk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2488. Dgkb.

Subcellular locationi

Cytoplasm. Membrane
Note: Can be loosely bound to the membranes.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 801801Diacylglycerol kinase beta
PRO_0000218458Add
BLAST

Proteomic databases

PRIDEiP49621.

PTM databases

PhosphoSiteiP49621.

Expressioni

Tissue specificityi

Confined to restricted neuronal populations such as the caudate-putamen, the accumbens nucleus, and the olfactory tubercle.

Gene expression databases

GenevestigatoriP49621.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058842.

Structurei

3D structure databases

ProteinModelPortaliP49621.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini148 – 18336EF-hand 1
Add
BLAST
Domaini193 – 22836EF-hand 2
Add
BLAST
Domaini431 – 565135DAGKc
Add
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.
Contains 2 EF-hand domains.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
HOGENOMiHOG000252931.
HOVERGENiHBG051345.
InParanoidiP49621.
KOiK00901.
PhylomeDBiP49621.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49621-1 [UniParc]FASTAAdd to Basket

« Hide

MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK    50
QDILNQTIDF EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPNVKS 100
KPALLSGGLR MNKGAITPPR SSPANTCFPE VIHLKDIVCY LSLLERGRPE 150
DKLEFMFRLY DTDGNGFLDS SELENIIGQM MHVAEYLEWD VTELNPILHE 200
MMEEIDYDRD GTVSLEEWIQ GGMTTIPLLV LLGLENNVKD DGQHVWRLKH 250
FNKPAYCNLC LNMLIGVGKQ GLCCSFCKYT VHERCARAPP SCIKTYVKSK 300
KNTDVMHHYW VEGNCPTKCD KCHKTVKCYQ GLTGLHCVWC QTTLHNKCAS 350
HLKPECDCGP LKDHILPPTT ICPVVLTMPT AGTSVPEERQ STAKKEKGSS 400
QQPNKVTDKN KMQRANSVTM DGQGLQITPI PGTHPLLVFV NPKSGGKQGE 450
RIYRKFQYLL NPRQVYSLSG NGPMPGLHFF RDVPDFRVLA CGGDGTVGWI 500
LDCIEKANVV KHPPVAILPL GTGNDLARCL RWGGGYEGEN LMKILKDIES 550
STEIMLDRWK FEVTPNDKDE KGDPVPYSII NNYFSIGVDA SIAHRFHIMR 600
EKHPEKFNSR MKNKFWYFEF GTSETFSATC KKLHESVEIE CDGVQIDLIN 650
ISLQGIAILN IPSMHGGSNL WGESKKKRSH RRIEKKGSDK RPTLTDAKEL 700
KFASQDLSDQ LLEVVGLEGA MEMGQIYTGL KSAGRRLAQC SSVVIRTSKS 750
LPMQIDGEPW MQTPCTIKIT HKNQAPMLMG PPPKTGLFCS LIKRTRNRSK 800
E 801
Length:801
Mass (Da):90,288
Last modified:February 1, 1996 - v1
Checksum:iF30874CD2DCE363D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16100 mRNA. Translation: BAA03675.1.
PIRiA47744.
RefSeqiNP_062177.1. NM_019304.1.
UniGeneiRn.11413.

Genome annotation databases

GeneIDi54248.
KEGGirno:54248.
UCSCiRGD:2488. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16100 mRNA. Translation: BAA03675.1 .
PIRi A47744.
RefSeqi NP_062177.1. NM_019304.1.
UniGenei Rn.11413.

3D structure databases

ProteinModelPortali P49621.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000058842.

PTM databases

PhosphoSitei P49621.

Proteomic databases

PRIDEi P49621.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 54248.
KEGGi rno:54248.
UCSCi RGD:2488. rat.

Organism-specific databases

CTDi 1607.
RGDi 2488. Dgkb.

Phylogenomic databases

eggNOGi NOG47311.
HOGENOMi HOG000252931.
HOVERGENi HBG051345.
InParanoidi P49621.
KOi K00901.
PhylomeDBi P49621.

Miscellaneous databases

NextBioi 610736.

Gene expression databases

Genevestigatori P49621.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view ]
Pfami PF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons."
    Goto K., Kondo H.
    Proc. Natl. Acad. Sci. U.S.A. 90:7598-7602(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.

Entry informationi

Entry nameiDGKB_RAT
AccessioniPrimary (citable) accession number: P49621
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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