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P49621

- DGKB_RAT

UniProt

P49621 - DGKB_RAT

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Protein

Diacylglycerol kinase beta

Gene

Dgkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Exhibits high phosphorylation activity for long-chain diacylglycerols.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by phosphatidylserine.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi161 – 172121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi206 – 217122PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri243 – 29250Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri307 – 35650Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. diacylglycerol kinase activity Source: RGD
  4. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase beta (EC:2.7.1.107)
Short name:
DAG kinase beta
Alternative name(s):
90 kDa diacylglycerol kinase
Diglyceride kinase beta
Short name:
DGK-beta
Gene namesi
Name:Dgkb
Synonyms:Dagk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2488. Dgkb.

Subcellular locationi

Cytoplasm. Membrane
Note: Can be loosely bound to the membranes.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 801801Diacylglycerol kinase betaPRO_0000218458Add
BLAST

Proteomic databases

PRIDEiP49621.

PTM databases

PhosphoSiteiP49621.

Expressioni

Tissue specificityi

Confined to restricted neuronal populations such as the caudate-putamen, the accumbens nucleus, and the olfactory tubercle.

Gene expression databases

GenevestigatoriP49621.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058842.

Structurei

3D structure databases

ProteinModelPortaliP49621.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini148 – 18336EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini193 – 22836EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini431 – 565135DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri243 – 29250Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri307 – 35650Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
HOGENOMiHOG000252931.
HOVERGENiHBG051345.
InParanoidiP49621.
KOiK00901.
PhylomeDBiP49621.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49621-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK
60 70 80 90 100
QDILNQTIDF EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPNVKS
110 120 130 140 150
KPALLSGGLR MNKGAITPPR SSPANTCFPE VIHLKDIVCY LSLLERGRPE
160 170 180 190 200
DKLEFMFRLY DTDGNGFLDS SELENIIGQM MHVAEYLEWD VTELNPILHE
210 220 230 240 250
MMEEIDYDRD GTVSLEEWIQ GGMTTIPLLV LLGLENNVKD DGQHVWRLKH
260 270 280 290 300
FNKPAYCNLC LNMLIGVGKQ GLCCSFCKYT VHERCARAPP SCIKTYVKSK
310 320 330 340 350
KNTDVMHHYW VEGNCPTKCD KCHKTVKCYQ GLTGLHCVWC QTTLHNKCAS
360 370 380 390 400
HLKPECDCGP LKDHILPPTT ICPVVLTMPT AGTSVPEERQ STAKKEKGSS
410 420 430 440 450
QQPNKVTDKN KMQRANSVTM DGQGLQITPI PGTHPLLVFV NPKSGGKQGE
460 470 480 490 500
RIYRKFQYLL NPRQVYSLSG NGPMPGLHFF RDVPDFRVLA CGGDGTVGWI
510 520 530 540 550
LDCIEKANVV KHPPVAILPL GTGNDLARCL RWGGGYEGEN LMKILKDIES
560 570 580 590 600
STEIMLDRWK FEVTPNDKDE KGDPVPYSII NNYFSIGVDA SIAHRFHIMR
610 620 630 640 650
EKHPEKFNSR MKNKFWYFEF GTSETFSATC KKLHESVEIE CDGVQIDLIN
660 670 680 690 700
ISLQGIAILN IPSMHGGSNL WGESKKKRSH RRIEKKGSDK RPTLTDAKEL
710 720 730 740 750
KFASQDLSDQ LLEVVGLEGA MEMGQIYTGL KSAGRRLAQC SSVVIRTSKS
760 770 780 790 800
LPMQIDGEPW MQTPCTIKIT HKNQAPMLMG PPPKTGLFCS LIKRTRNRSK

E
Length:801
Mass (Da):90,288
Last modified:February 1, 1996 - v1
Checksum:iF30874CD2DCE363D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16100 mRNA. Translation: BAA03675.1.
PIRiA47744.
RefSeqiNP_062177.1. NM_019304.1.
UniGeneiRn.11413.

Genome annotation databases

GeneIDi54248.
KEGGirno:54248.
UCSCiRGD:2488. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16100 mRNA. Translation: BAA03675.1 .
PIRi A47744.
RefSeqi NP_062177.1. NM_019304.1.
UniGenei Rn.11413.

3D structure databases

ProteinModelPortali P49621.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000058842.

PTM databases

PhosphoSitei P49621.

Proteomic databases

PRIDEi P49621.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 54248.
KEGGi rno:54248.
UCSCi RGD:2488. rat.

Organism-specific databases

CTDi 1607.
RGDi 2488. Dgkb.

Phylogenomic databases

eggNOGi NOG47311.
HOGENOMi HOG000252931.
HOVERGENi HBG051345.
InParanoidi P49621.
KOi K00901.
PhylomeDBi P49621.

Miscellaneous databases

NextBioi 610736.

Gene expression databases

Genevestigatori P49621.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. PE/DAG-bd.
[Graphical view ]
Pfami PF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons."
    Goto K., Kondo H.
    Proc. Natl. Acad. Sci. U.S.A. 90:7598-7602(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.

Entry informationi

Entry nameiDGKB_RAT
AccessioniPrimary (citable) accession number: P49621
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3