P49620 (DGKG_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase gamma
Short name=DAG kinase gamma
EC=2.7.1.107

Alternative name(s):
88 kDa diacylglycerol kinase
Diglyceride kinase gamma
Short name=DGK-gamma

Gene names

Name:	Dgkg
Synonyms:	Dagk3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	788 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Tissue specificity	Expressed specifically in brain and localized predominantly in cerebellar Purkinje cells.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 EF-hand domains. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Traceable author statement Ref.1. Source: RGD intracellular signal transduction Inferred from electronic annotation. Source: InterPro neuron development Inferred from expression pattern. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: InterPro diacylglycerol kinase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 788	788	Diacylglycerol kinase gamma		PRO_0000218461
Regions
	Domain	172 – 207	36	EF-hand 1
	Domain	217 – 252	36	EF-hand 2
	Domain	427 – 561	135	DAGKc
	Calcium binding	185 – 196	12	1 Potential
	Calcium binding	230 – 241	12	2 Potential
	Zinc finger	268 – 318	51	Phorbol-ester/DAG-type 1
	Zinc finger	333 – 380	48	Phorbol-ester/DAG-type 2

Sequences

	Sequence		Length	Mass (Da)
	P49620 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: E77BCA40B22C1DA9	FASTA	788	88,521
	10 20 30 40 50 60 MSDGQWVCLS SEEFDQLQKY SEYSSKKIKD VLAEFNEGGS LKQYDPHKPI SYDVFKLFMR 70 80 90 100 110 120 AYLEVDLPQP LSTNLFLAFS QKPRQETPDH PKEGASSSEP NVSDSNAEST AKADAACAPD 130 140 150 160 170 180 TESKPIKTQV PSEELEAAAP WGEPNAPASS SDAPIVYLKD VVCYLSLMET GRPQDKLEFM 190 200 210 220 230 240 FRLYDSDENE LLDQAELDQI VSQMLHVAQY LEWDPTELRP ILKEMLQGMD YNKDGFVSLE 250 260 270 280 290 300 EWVSGGMTTI PLLVLLGMDD SASKGDGRHA WTLKHFKKPT YCNFCHIMLM GVRKQGLCCI 310 320 330 340 350 360 YCKYAVHQRC VSNSIPGCVK TYSKAKRSGE VMQHAWVEGN SSVKCDRCHK SIKCYQSVTA 370 380 390 400 410 420 RHCVWCRMTF HRKCELSTAC DGGELKDHIL LPTSIYPVTR DRQAGKSDSG AAAKGELVMQ 430 440 450 460 470 480 YKIIPSPGTH PLLVLVNPKS GGRQGERILQ KFHYLLNPKQ VFNLDKGGPT PGLNFFQDTP 490 500 510 520 530 540 DFRVLACGGD GTVGWILDCI DKANFTKHPP VAVLPLGTGN DLARCLRWGG GYEGGSLTKI 550 560 570 580 590 600 LKEIEQSPLV MLDRWYLEVM PREEVENGDQ VPYNIMNNYF SIGVDASIAH RFHVMREKHP 610 620 630 640 650 660 EKFNSRMKNK LWYFEFGTSE TFAATCKKLH DHIELECDGV EVDLSNIFLE GIAILNIPSM 670 680 690 700 710 720 YGGTNLWGET KKNRAVIRES RKSVTDPKEL KCCVQDLSDQ LLEVVGLEGA MEMGQIYTGL 730 740 750 760 770 780 KSAGRRLAQC SSVTIRTKKL LPMQVDGEPW MQPPCMIKIT HKNQAPMMMG PPQKSSFFSL RRKSRSKD

References

[1]	"Cloning and expression of a cytoskeleton-associated diacylglycerol kinase that is dominantly expressed in cerebellum." Goto K., Funayama M., Kondo H. Proc. Natl. Acad. Sci. U.S.A. 91:13042-13046(1994) [PubMed: 7809169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38448 mRNA. Translation: BAA07480.1.
IPI	IPI00196194.
RefSeq	NP_037258.1. NM_013126.1.
UniGene	Rn.10544.
3D structure databases
ProteinModelPortal	P49620.
ModBase	Search...
Protein-protein interaction databases
STRING	P49620.
PTM databases
PhosphoSite	P49620.
Proteomic databases
PRIDE	P49620.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000002452; ENSRNOP00000002452; ENSRNOG00000001796.
GeneID	25666.
KEGG	rno:25666.
Organism-specific databases
CTD	1608.
RGD	2499. Dgkg.
Phylogenomic databases
eggNOG	roNOG04537.
GeneTree	ENSGT00600000084185.
HOVERGEN	HBG051345.
InParanoid	P49620.
OrthoDB	EOG4GHZNH.
Gene expression databases
ArrayExpress	P49620.
Genevestigator	P49620.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
KO	K00901.
Pfam	PF00130. C1_1. 1 hit. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	607585.

Entry information

Entry name

DGKG_RAT

Accession

Primary (citable) accession number: P49620

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 16, 2011
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program