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Protein

Diacylglycerol kinase gamma

Gene

Dgkg

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi185 – 196121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi230 – 241122PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri268 – 31851Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri333 – 38048Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. diacylglycerol kinase activity Source: RGD
  4. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. neuron development Source: RGD
  3. protein kinase C-activating G-protein coupled receptor signaling pathway Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_351116. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase gamma (EC:2.7.1.107)
Short name:
DAG kinase gamma
Alternative name(s):
88 kDa diacylglycerol kinase
Diglyceride kinase gamma
Short name:
DGK-gamma
Gene namesi
Name:Dgkg
Synonyms:Dagk3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi2499. Dgkg.

Subcellular locationi

GO - Cellular componenti

  1. intracellular membrane-bounded organelle Source: Ensembl
  2. plasma membrane Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 788788Diacylglycerol kinase gammaPRO_0000218461Add
BLAST

Proteomic databases

PaxDbiP49620.
PRIDEiP49620.

PTM databases

PhosphoSiteiP49620.

Expressioni

Tissue specificityi

Expressed specifically in brain and localized predominantly in cerebellar Purkinje cells.

Gene expression databases

GenevestigatoriP49620.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002452.

Structurei

3D structure databases

ProteinModelPortaliP49620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 20736EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini217 – 25236EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini427 – 561135DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri268 – 31851Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri333 – 38048Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000252931.
HOVERGENiHBG051345.
InParanoidiP49620.
KOiK00901.
OMAiCYQSVTA.
OrthoDBiEOG75XGK8.
PhylomeDBiP49620.
TreeFamiTF313104.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 2 hits.
InterProiIPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49620-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDGQWVCLS SEEFDQLQKY SEYSSKKIKD VLAEFNEGGS LKQYDPHKPI
60 70 80 90 100
SYDVFKLFMR AYLEVDLPQP LSTNLFLAFS QKPRQETPDH PKEGASSSEP
110 120 130 140 150
NVSDSNAEST AKADAACAPD TESKPIKTQV PSEELEAAAP WGEPNAPASS
160 170 180 190 200
SDAPIVYLKD VVCYLSLMET GRPQDKLEFM FRLYDSDENE LLDQAELDQI
210 220 230 240 250
VSQMLHVAQY LEWDPTELRP ILKEMLQGMD YNKDGFVSLE EWVSGGMTTI
260 270 280 290 300
PLLVLLGMDD SASKGDGRHA WTLKHFKKPT YCNFCHIMLM GVRKQGLCCI
310 320 330 340 350
YCKYAVHQRC VSNSIPGCVK TYSKAKRSGE VMQHAWVEGN SSVKCDRCHK
360 370 380 390 400
SIKCYQSVTA RHCVWCRMTF HRKCELSTAC DGGELKDHIL LPTSIYPVTR
410 420 430 440 450
DRQAGKSDSG AAAKGELVMQ YKIIPSPGTH PLLVLVNPKS GGRQGERILQ
460 470 480 490 500
KFHYLLNPKQ VFNLDKGGPT PGLNFFQDTP DFRVLACGGD GTVGWILDCI
510 520 530 540 550
DKANFTKHPP VAVLPLGTGN DLARCLRWGG GYEGGSLTKI LKEIEQSPLV
560 570 580 590 600
MLDRWYLEVM PREEVENGDQ VPYNIMNNYF SIGVDASIAH RFHVMREKHP
610 620 630 640 650
EKFNSRMKNK LWYFEFGTSE TFAATCKKLH DHIELECDGV EVDLSNIFLE
660 670 680 690 700
GIAILNIPSM YGGTNLWGET KKNRAVIRES RKSVTDPKEL KCCVQDLSDQ
710 720 730 740 750
LLEVVGLEGA MEMGQIYTGL KSAGRRLAQC SSVTIRTKKL LPMQVDGEPW
760 770 780
MQPPCMIKIT HKNQAPMMMG PPQKSSFFSL RRKSRSKD
Length:788
Mass (Da):88,521
Last modified:February 1, 1996 - v1
Checksum:iE77BCA40B22C1DA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38448 mRNA. Translation: BAA07480.1.
RefSeqiNP_037258.1. NM_013126.1.
UniGeneiRn.10544.

Genome annotation databases

EnsembliENSRNOT00000002452; ENSRNOP00000002452; ENSRNOG00000001796.
GeneIDi25666.
KEGGirno:25666.
UCSCiRGD:2499. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38448 mRNA. Translation: BAA07480.1.
RefSeqiNP_037258.1. NM_013126.1.
UniGeneiRn.10544.

3D structure databases

ProteinModelPortaliP49620.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002452.

PTM databases

PhosphoSiteiP49620.

Proteomic databases

PaxDbiP49620.
PRIDEiP49620.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002452; ENSRNOP00000002452; ENSRNOG00000001796.
GeneIDi25666.
KEGGirno:25666.
UCSCiRGD:2499. rat.

Organism-specific databases

CTDi1608.
RGDi2499. Dgkg.

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000252931.
HOVERGENiHBG051345.
InParanoidiP49620.
KOiK00901.
OMAiCYQSVTA.
OrthoDBiEOG75XGK8.
PhylomeDBiP49620.
TreeFamiTF313104.

Enzyme and pathway databases

ReactomeiREACT_351116. Effects of PIP2 hydrolysis.

Miscellaneous databases

NextBioi607585.
PROiP49620.

Gene expression databases

GenevestigatoriP49620.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 2 hits.
InterProiIPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of a cytoskeleton-associated diacylglycerol kinase that is dominantly expressed in cerebellum."
    Goto K., Funayama M., Kondo H.
    Proc. Natl. Acad. Sci. U.S.A. 91:13042-13046(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.

Entry informationi

Entry nameiDGKG_RAT
AccessioniPrimary (citable) accession number: P49620
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 29, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.