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Protein

Diacylglycerol kinase gamma

Gene

DGKG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Reverses the normal flow of glycerolipid biosynthesis by phosphorylating diacylglycerol back to phosphatidic acid.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Requires phosphatidylserine for maximal activity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi188 – 1991PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi233 – 2442PROSITE-ProRule annotationAdd BLAST12
Zinc fingeri271 – 321Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri336 – 385Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium ion binding Source: InterPro
  • diacylglycerol kinase activity Source: BHF-UCL

GO - Biological processi

  • glycerolipid metabolic process Source: BHF-UCL
  • intracellular signal transduction Source: InterPro
  • lipid phosphorylation Source: BHF-UCL
  • neuron development Source: Ensembl
  • platelet activation Source: Reactome
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  • signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS00726-MONOMER.
BRENDAi2.7.1.107. 2681.
ReactomeiR-HSA-114508. Effects of PIP2 hydrolysis.
SABIO-RKP49619.

Chemistry databases

SwissLipidsiSLP:000000923.
SLP:000000938. [P49619-1]

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase gamma (EC:2.7.1.107)
Short name:
DAG kinase gamma
Alternative name(s):
Diglyceride kinase gamma
Short name:
DGK-gamma
Gene namesi
Name:DGKG
Synonyms:DAGK3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:2853. DGKG.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi1608.
OpenTargetsiENSG00000058866.
PharmGKBiPA27314.

Chemistry databases

ChEMBLiCHEMBL1075157.
DrugBankiDB00144. Phosphatidylserine.

Polymorphism and mutation databases

BioMutaiDGKG.
DMDMi311033457.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002184591 – 791Diacylglycerol kinase gammaAdd BLAST791

Proteomic databases

EPDiP49619.
PaxDbiP49619.
PeptideAtlasiP49619.
PRIDEiP49619.

PTM databases

iPTMnetiP49619.
PhosphoSitePlusiP49619.

Expressioni

Tissue specificityi

Predominantly expressed in retina and in a much lesser extent in the brain. Other tissues contain extremely low levels of DGK-gamma.

Gene expression databases

BgeeiENSG00000058866.
CleanExiHS_DGKG.
ExpressionAtlasiP49619. baseline and differential.
GenevisibleiP49619. HS.

Organism-specific databases

HPAiHPA036577.

Interactioni

Protein-protein interaction databases

BioGridi107978. 2 interactors.
IntActiP49619. 2 interactors.
STRINGi9606.ENSP00000265022.

Structurei

3D structure databases

ProteinModelPortaliP49619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini175 – 210EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini220 – 255EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini430 – 564DAGKcPROSITE-ProRule annotationAdd BLAST135

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi151 – 156Poly-Ser6

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri271 – 321Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri336 – 385Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169. Eukaryota.
ENOG410XQVB. LUCA.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000252931.
HOVERGENiHBG051345.
InParanoidiP49619.
KOiK00901.
OMAiCYQSVTA.
OrthoDBiEOG091G04A5.
PhylomeDBiP49619.
TreeFamiTF313104.

Family and domain databases

CDDicd00029. C1. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 2 hits.
InterProiIPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF47473. SSF47473. 3 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P49619-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEERWVSLT PEEFDQLQKY SEYSSKKIKD ALTEFNEGGS LKQYDPHEPI
60 70 80 90 100
SYDVFKLFMR AYLEVDLPQP LSTHLFLAFS QKPRHETSDH PTEGASNSEA
110 120 130 140 150
NSADTNIQNA DNATKADEAC APDTESNMAE KQAPAEDQVA ATPLEPPVPR
160 170 180 190 200
SSSSESPVVY LKDVVCYLSL LETGRPQDKL EFMFRLYDSD ENGLLDQAEM
210 220 230 240 250
DCIVNQMLHI AQYLEWDPTE LRPILKEMLQ GMDYDRDGFV SLQEWVHGGM
260 270 280 290 300
TTIPLLVLLG MDDSGSKGDG RHAWTMKHFK KPTYCNFCHI MLMGVRKQGL
310 320 330 340 350
CCTYCKYTVH ERCVSRNIPG CVKTYSKAKR SGEVMQHAWV EGNSSVKCDR
360 370 380 390 400
CHKSIKCYQS VTARHCVWCR MTFHRKCELS TLCDGGELRD HILLPTSICP
410 420 430 440 450
ITRDRPGEKS DGCVSAKGEL VMQYKIIPTP GTHPLLVLVN PKSGGRQGER
460 470 480 490 500
ILRKFHYLLN PKQVFNLDNG GPTPGLNFFR DTPDFRVLAC GGDGTVGWIL
510 520 530 540 550
DCIDKANFAK HPPVAVLPLG TGNDLARCLR WGGGYEGGSL TKILKDIEQS
560 570 580 590 600
PLVMLDRWHL EVIPREEVEN GDQVPYSIMN NYFSIGVDAS IAHRFHVMRE
610 620 630 640 650
KHPEKFNSRM KNKLWYFEFG TSETFAATCK KLHDHIELEC DGVGVDLSNI
660 670 680 690 700
FLEGIAILNI PSMYGGTNLW GENKKNRAVI RESRKGVTDP KELKFCVQDL
710 720 730 740 750
SDQLLEVVGL EGAMEMGQIY TGLKSAGRRL AQCASVTIRT NKLLPMQVDG
760 770 780 790
EPWMQPCCTI KITHKNQAPM MMGPPQKSSF FSLRRKSRSK D
Length:791
Mass (Da):89,124
Last modified:November 2, 2010 - v3
Checksum:iA1382CF1B2CD7362
GO
Isoform 2 (identifier: P49619-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     451-475: Missing.

Note: May be inactive.
Show »
Length:766
Mass (Da):86,303
Checksum:i1D3F8C78BD59219F
GO
Isoform 3 (identifier: P49619-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     334-372: Missing.

Show »
Length:752
Mass (Da):84,573
Checksum:i4B58648DC4E785ED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti271R → G in BAA05132 (PubMed:8034597).Curated1
Sequence conflicti271R → G in AAC04686 (PubMed:10071200).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020259142T → S.2 PublicationsCorresponds to variant rs1004588dbSNPEnsembl.1
Natural variantiVAR_024430316R → K.4 PublicationsCorresponds to variant rs2193587dbSNPEnsembl.1
Natural variantiVAR_020260370R → W.Corresponds to variant rs3213770dbSNPEnsembl.1
Natural variantiVAR_036119706E → K in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039922334 – 372Missing in isoform 3. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_001267451 – 475Missing in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26135 mRNA. Translation: BAA05132.1.
AF020945
, AF020922, AF020923, AF020924, AF020925, AF020926, AF020927, AF020928, AF020929, AF020930, AF020931, AF020932, AF020933, AF020934, AF020935, AF020936, AF020937, AF020938, AF020939, AF020940, AF020941, AF020942, AF020943, AF020944 Genomic DNA. Translation: AAC04686.1.
AK314192 mRNA. Translation: BAG36871.1.
AC007917 Genomic DNA. No translation available.
AC112649 Genomic DNA. No translation available.
BC089411 mRNA. Translation: AAH89411.1.
BC112363 mRNA. Translation: AAI12364.1.
CCDSiCCDS3274.1. [P49619-1]
CCDS43181.1. [P49619-3]
CCDS43182.1. [P49619-2]
PIRiA53691.
RefSeqiNP_001074213.1. NM_001080744.1. [P49619-2]
NP_001074214.1. NM_001080745.1. [P49619-3]
NP_001337.2. NM_001346.2. [P49619-1]
UniGeneiHs.683449.

Genome annotation databases

EnsembliENST00000265022; ENSP00000265022; ENSG00000058866. [P49619-1]
ENST00000344484; ENSP00000339777; ENSG00000058866. [P49619-2]
ENST00000382164; ENSP00000371599; ENSG00000058866. [P49619-3]
GeneIDi1608.
KEGGihsa:1608.
UCSCiuc003fqa.4. human. [P49619-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26135 mRNA. Translation: BAA05132.1.
AF020945
, AF020922, AF020923, AF020924, AF020925, AF020926, AF020927, AF020928, AF020929, AF020930, AF020931, AF020932, AF020933, AF020934, AF020935, AF020936, AF020937, AF020938, AF020939, AF020940, AF020941, AF020942, AF020943, AF020944 Genomic DNA. Translation: AAC04686.1.
AK314192 mRNA. Translation: BAG36871.1.
AC007917 Genomic DNA. No translation available.
AC112649 Genomic DNA. No translation available.
BC089411 mRNA. Translation: AAH89411.1.
BC112363 mRNA. Translation: AAI12364.1.
CCDSiCCDS3274.1. [P49619-1]
CCDS43181.1. [P49619-3]
CCDS43182.1. [P49619-2]
PIRiA53691.
RefSeqiNP_001074213.1. NM_001080744.1. [P49619-2]
NP_001074214.1. NM_001080745.1. [P49619-3]
NP_001337.2. NM_001346.2. [P49619-1]
UniGeneiHs.683449.

3D structure databases

ProteinModelPortaliP49619.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107978. 2 interactors.
IntActiP49619. 2 interactors.
STRINGi9606.ENSP00000265022.

Chemistry databases

ChEMBLiCHEMBL1075157.
DrugBankiDB00144. Phosphatidylserine.
SwissLipidsiSLP:000000923.
SLP:000000938. [P49619-1]

PTM databases

iPTMnetiP49619.
PhosphoSitePlusiP49619.

Polymorphism and mutation databases

BioMutaiDGKG.
DMDMi311033457.

Proteomic databases

EPDiP49619.
PaxDbiP49619.
PeptideAtlasiP49619.
PRIDEiP49619.

Protocols and materials databases

DNASUi1608.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265022; ENSP00000265022; ENSG00000058866. [P49619-1]
ENST00000344484; ENSP00000339777; ENSG00000058866. [P49619-2]
ENST00000382164; ENSP00000371599; ENSG00000058866. [P49619-3]
GeneIDi1608.
KEGGihsa:1608.
UCSCiuc003fqa.4. human. [P49619-1]

Organism-specific databases

CTDi1608.
DisGeNETi1608.
GeneCardsiDGKG.
HGNCiHGNC:2853. DGKG.
HPAiHPA036577.
MIMi601854. gene.
neXtProtiNX_P49619.
OpenTargetsiENSG00000058866.
PharmGKBiPA27314.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1169. Eukaryota.
ENOG410XQVB. LUCA.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000252931.
HOVERGENiHBG051345.
InParanoidiP49619.
KOiK00901.
OMAiCYQSVTA.
OrthoDBiEOG091G04A5.
PhylomeDBiP49619.
TreeFamiTF313104.

Enzyme and pathway databases

BioCyciZFISH:HS00726-MONOMER.
BRENDAi2.7.1.107. 2681.
ReactomeiR-HSA-114508. Effects of PIP2 hydrolysis.
SABIO-RKP49619.

Miscellaneous databases

ChiTaRSiDGKG. human.
GeneWikiiDGKG.
GenomeRNAii1608.
PROiP49619.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000058866.
CleanExiHS_DGKG.
ExpressionAtlasiP49619. baseline and differential.
GenevisibleiP49619. HS.

Family and domain databases

CDDicd00029. C1. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 2 hits.
InterProiIPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF47473. SSF47473. 3 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDGKG_HUMAN
AccessioniPrimary (citable) accession number: P49619
Secondary accession number(s): B2RAH4, Q2M1H4, Q5FWG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 2, 2010
Last modified: November 30, 2016
This is version 165 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.