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P49619 (DGKG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diacylglycerol kinase gamma

Short name=DAG kinase gamma
EC=2.7.1.107
Alternative name(s):
Diglyceride kinase gamma
Short name=DGK-gamma
Gene names
Name:DGKG
Synonyms:DAGK3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length791 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Reverses the normal flow of glycerolipid biosynthesis by phosphorylating diacylglycerol back to phosphatidic acid.

Catalytic activity

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulation

Requires phosphatidylserine for maximal activity.

Subcellular location

Cytoplasm. Membrane. Note: Can be loosely bound to the membranes.

Tissue specificity

Predominantly expressed in retina and in a much lesser extent in the brain. Other tissues contain extremely low levels of DGK-gamma.

Sequence similarities

Belongs to the eukaryotic diacylglycerol kinase family.

Contains 1 DAGKc domain.

Contains 2 EF-hand domains.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P49619-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P49619-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     451-475: Missing.
Note: May be inactive.
Isoform 3 (identifier: P49619-3)

The sequence of this isoform differs from the canonical sequence as follows:
     334-372: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 791791Diacylglycerol kinase gamma
PRO_0000218459

Regions

Domain175 – 21036EF-hand 1
Domain220 – 25536EF-hand 2
Domain430 – 564135DAGKc
Calcium binding188 – 199121 Potential
Calcium binding233 – 244122 Potential
Zinc finger271 – 32151Phorbol-ester/DAG-type 1
Zinc finger336 – 38550Phorbol-ester/DAG-type 2
Compositional bias151 – 1566Poly-Ser

Natural variations

Alternative sequence334 – 37239Missing in isoform 3.
VSP_039922
Alternative sequence451 – 47525Missing in isoform 2.
VSP_001267
Natural variant1421T → S. Ref.3 Ref.5
Corresponds to variant rs1004588 [ dbSNP | Ensembl ].
VAR_020259
Natural variant3161R → K. Ref.1 Ref.2 Ref.3 Ref.5
Corresponds to variant rs2193587 [ dbSNP | Ensembl ].
VAR_024430
Natural variant3701R → W.
Corresponds to variant rs3213770 [ dbSNP | Ensembl ].
VAR_020260
Natural variant7061E → K in a breast cancer sample; somatic mutation. Ref.6
VAR_036119

Experimental info

Sequence conflict2711R → G in BAA05132. Ref.1
Sequence conflict2711R → G in AAC04686. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) [UniParc].

Last modified November 2, 2010. Version 3.
Checksum: A1382CF1B2CD7362

FASTA79189,124
        10         20         30         40         50         60 
MGEERWVSLT PEEFDQLQKY SEYSSKKIKD ALTEFNEGGS LKQYDPHEPI SYDVFKLFMR 

        70         80         90        100        110        120 
AYLEVDLPQP LSTHLFLAFS QKPRHETSDH PTEGASNSEA NSADTNIQNA DNATKADEAC 

       130        140        150        160        170        180 
APDTESNMAE KQAPAEDQVA ATPLEPPVPR SSSSESPVVY LKDVVCYLSL LETGRPQDKL 

       190        200        210        220        230        240 
EFMFRLYDSD ENGLLDQAEM DCIVNQMLHI AQYLEWDPTE LRPILKEMLQ GMDYDRDGFV 

       250        260        270        280        290        300 
SLQEWVHGGM TTIPLLVLLG MDDSGSKGDG RHAWTMKHFK KPTYCNFCHI MLMGVRKQGL 

       310        320        330        340        350        360 
CCTYCKYTVH ERCVSRNIPG CVKTYSKAKR SGEVMQHAWV EGNSSVKCDR CHKSIKCYQS 

       370        380        390        400        410        420 
VTARHCVWCR MTFHRKCELS TLCDGGELRD HILLPTSICP ITRDRPGEKS DGCVSAKGEL 

       430        440        450        460        470        480 
VMQYKIIPTP GTHPLLVLVN PKSGGRQGER ILRKFHYLLN PKQVFNLDNG GPTPGLNFFR 

       490        500        510        520        530        540 
DTPDFRVLAC GGDGTVGWIL DCIDKANFAK HPPVAVLPLG TGNDLARCLR WGGGYEGGSL 

       550        560        570        580        590        600 
TKILKDIEQS PLVMLDRWHL EVIPREEVEN GDQVPYSIMN NYFSIGVDAS IAHRFHVMRE 

       610        620        630        640        650        660 
KHPEKFNSRM KNKLWYFEFG TSETFAATCK KLHDHIELEC DGVGVDLSNI FLEGIAILNI 

       670        680        690        700        710        720 
PSMYGGTNLW GENKKNRAVI RESRKGVTDP KELKFCVQDL SDQLLEVVGL EGAMEMGQIY 

       730        740        750        760        770        780 
TGLKSAGRRL AQCASVTIRT NKLLPMQVDG EPWMQPCCTI KITHKNQAPM MMGPPQKSSF 

       790 
FSLRRKSRSK D 

« Hide

Isoform 2 (Short) [UniParc].

Checksum: 1D3F8C78BD59219F
Show »

FASTA76686,303
Isoform 3 [UniParc].

Checksum: 4B58648DC4E785ED
Show »

FASTA75284,573

References

« Hide 'large scale' references
[1]"Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells."
Kai M., Sakane F., Imai S., Wada I., Kanoh H.
J. Biol. Chem. 269:18492-18498(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT LYS-316.
Tissue: Liver.
[2]"Mapping and genomic characterization of the gene encoding diacylglycerol kinase gamma (DAGK3): assessment of its role in dominant optic atrophy (OPA1)."
Stoehr H., Klein J., Gehrig A., Koehler M.R., Jurklies B., Kellner U., Leo-Kottler B., Schmid M., Weber B.H.F.
Hum. Genet. 104:99-105(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LYS-316.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-142 AND LYS-316.
Tissue: Thalamus.
[4]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), VARIANTS SER-142 AND LYS-316.
Tissue: Lymph.
[6]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] LYS-706.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D26135 mRNA. Translation: BAA05132.1.
AF020945 expand/collapse EMBL AC list , AF020922, AF020923, AF020924, AF020925, AF020926, AF020927, AF020928, AF020929, AF020930, AF020931, AF020932, AF020933, AF020934, AF020935, AF020936, AF020937, AF020938, AF020939, AF020940, AF020941, AF020942, AF020943, AF020944 Genomic DNA. Translation: AAC04686.1.
AK314192 mRNA. Translation: BAG36871.1.
AC007917 Genomic DNA. No translation available.
AC112649 Genomic DNA. No translation available.
BC089411 mRNA. Translation: AAH89411.1.
BC112363 mRNA. Translation: AAI12364.1.
PIRA53691.
RefSeqNP_001074213.1. NM_001080744.1.
NP_001074214.1. NM_001080745.1.
NP_001337.2. NM_001346.2.
UniGeneHs.683449.

3D structure databases

ProteinModelPortalP49619.
SMRP49619. Positions 1-323, 333-383.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107978. 2 interactions.
IntActP49619. 2 interactions.
STRING9606.ENSP00000265022.

Chemistry

ChEMBLCHEMBL1075157.
DrugBankDB00144. Phosphatidylserine.

PTM databases

PhosphoSiteP49619.

Polymorphism databases

DMDM311033457.

Proteomic databases

PaxDbP49619.
PRIDEP49619.

Protocols and materials databases

DNASU1608.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000265022; ENSP00000265022; ENSG00000058866. [P49619-1]
ENST00000344484; ENSP00000339777; ENSG00000058866. [P49619-2]
ENST00000382164; ENSP00000371599; ENSG00000058866. [P49619-3]
GeneID1608.
KEGGhsa:1608.
UCSCuc003fqa.3. human. [P49619-1]
uc003fqb.3. human. [P49619-3]
uc003fqc.3. human. [P49619-2]

Organism-specific databases

CTD1608.
GeneCardsGC03M185823.
HGNCHGNC:2853. DGKG.
HPAHPA036577.
MIM601854. gene.
neXtProtNX_P49619.
PharmGKBPA27314.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG47311.
HOGENOMHOG000252931.
HOVERGENHBG051345.
InParanoidP49619.
KOK00901.
OMACYQSVTA.
OrthoDBEOG75XGK8.
PhylomeDBP49619.
TreeFamTF313104.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_604. Hemostasis.
SABIO-RKP49619.

Gene expression databases

ArrayExpressP49619.
BgeeP49619.
CleanExHS_DGKG.
GenevestigatorP49619.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
PfamPF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
PROSITEPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiDGKG.
GenomeRNAi1608.
NextBio6606.
PROP49619.
SOURCESearch...

Entry information

Entry nameDGKG_HUMAN
AccessionPrimary (citable) accession number: P49619
Secondary accession number(s): B2RAH4, Q2M1H4, Q5FWG1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 2, 2010
Last modified: April 16, 2014
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM