P49619 (DGKG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase gamma
Short name=DAG kinase gamma
EC=2.7.1.107

Alternative name(s):
Diglyceride kinase gamma
Short name=DGK-gamma

Gene names

Name:	DGKG
Synonyms:	DAGK3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	791 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Reverses the normal flow of glycerolipid biosynthesis by phosphorylating diacylglycerol back to phosphatidic acid.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Enzyme regulation	Requires phosphatidylserine for maximal activity.
Subcellular location	Cytoplasm. Membrane. Note: Can be loosely bound to the membranes.
Tissue specificity	Predominantly expressed in retina and in a much lesser extent in the brain. Other tissues contain extremely low levels of DGK-gamma.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 EF-hand domains. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro neuron development Inferred from electronic annotation. Source: Compara platelet activation Traceable author statement. Source: Reactome protein kinase C-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro signal transduction Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: InterPro diacylglycerol kinase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P49619-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P49619-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     451-475: Missing.

Note: May be inactive.

Isoform 3 (identifier: P49619-3)

The sequence of this isoform differs from the canonical sequence as follows:
     334-372: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 791	791	Diacylglycerol kinase gamma		PRO_0000218459
Regions
	Domain	175 – 210	36	EF-hand 1
	Domain	220 – 255	36	EF-hand 2
	Domain	430 – 564	135	DAGKc
	Calcium binding	188 – 199	12	1 Potential
	Calcium binding	233 – 244	12	2 Potential
	Zinc finger	271 – 321	51	Phorbol-ester/DAG-type 1
	Zinc finger	336 – 385	50	Phorbol-ester/DAG-type 2
	Compositional bias	151 – 156	6	Poly-Ser
Natural variations
	Alternative sequence	334 – 372	39	Missing in isoform 3.		VSP_039922
	Alternative sequence	451 – 475	25	Missing in isoform 2.		VSP_001267
	Natural variant	142	1	T → S. Ref.3 Ref.5 Corresponds to variant rs1004588 [ dbSNP | Ensembl ].		VAR_020259
	Natural variant	316	1	R → K. Ref.1 Ref.2 Ref.3 Ref.5 Corresponds to variant rs2193587 [ dbSNP | Ensembl ].		VAR_024430
	Natural variant	370	1	R → W. Corresponds to variant rs3213770 [ dbSNP | Ensembl ].		VAR_020260
	Natural variant	706	1	E → K in a breast cancer sample; somatic mutation. Ref.6		VAR_036119
Experimental info
	Sequence conflict	271	1	R → G in BAA05132. Ref.1
	Sequence conflict	271	1	R → G in AAC04686. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) [UniParc]. Last modified November 2, 2010. Version 3. Checksum: A1382CF1B2CD7362	FASTA	791	89,124
	10 20 30 40 50 60 MGEERWVSLT PEEFDQLQKY SEYSSKKIKD ALTEFNEGGS LKQYDPHEPI SYDVFKLFMR 70 80 90 100 110 120 AYLEVDLPQP LSTHLFLAFS QKPRHETSDH PTEGASNSEA NSADTNIQNA DNATKADEAC 130 140 150 160 170 180 APDTESNMAE KQAPAEDQVA ATPLEPPVPR SSSSESPVVY LKDVVCYLSL LETGRPQDKL 190 200 210 220 230 240 EFMFRLYDSD ENGLLDQAEM DCIVNQMLHI AQYLEWDPTE LRPILKEMLQ GMDYDRDGFV 250 260 270 280 290 300 SLQEWVHGGM TTIPLLVLLG MDDSGSKGDG RHAWTMKHFK KPTYCNFCHI MLMGVRKQGL 310 320 330 340 350 360 CCTYCKYTVH ERCVSRNIPG CVKTYSKAKR SGEVMQHAWV EGNSSVKCDR CHKSIKCYQS 370 380 390 400 410 420 VTARHCVWCR MTFHRKCELS TLCDGGELRD HILLPTSICP ITRDRPGEKS DGCVSAKGEL 430 440 450 460 470 480 VMQYKIIPTP GTHPLLVLVN PKSGGRQGER ILRKFHYLLN PKQVFNLDNG GPTPGLNFFR 490 500 510 520 530 540 DTPDFRVLAC GGDGTVGWIL DCIDKANFAK HPPVAVLPLG TGNDLARCLR WGGGYEGGSL 550 560 570 580 590 600 TKILKDIEQS PLVMLDRWHL EVIPREEVEN GDQVPYSIMN NYFSIGVDAS IAHRFHVMRE 610 620 630 640 650 660 KHPEKFNSRM KNKLWYFEFG TSETFAATCK KLHDHIELEC DGVGVDLSNI FLEGIAILNI 670 680 690 700 710 720 PSMYGGTNLW GENKKNRAVI RESRKGVTDP KELKFCVQDL SDQLLEVVGL EGAMEMGQIY 730 740 750 760 770 780 TGLKSAGRRL AQCASVTIRT NKLLPMQVDG EPWMQPCCTI KITHKNQAPM MMGPPQKSSF 790 FSLRRKSRSK D
	Isoform 2 (Short) [UniParc]. Checksum: 1D3F8C78BD59219F	FASTA	766	86,303
	10 20 30 40 50 60 MGEERWVSLT PEEFDQLQKY SEYSSKKIKD ALTEFNEGGS LKQYDPHEPI SYDVFKLFMR 70 80 90 100 110 120 AYLEVDLPQP LSTHLFLAFS QKPRHETSDH PTEGASNSEA NSADTNIQNA DNATKADEAC 130 140 150 160 170 180 APDTESNMAE KQAPAEDQVA ATPLEPPVPR SSSSESPVVY LKDVVCYLSL LETGRPQDKL 190 200 210 220 230 240 EFMFRLYDSD ENGLLDQAEM DCIVNQMLHI AQYLEWDPTE LRPILKEMLQ GMDYDRDGFV 250 260 270 280 290 300 SLQEWVHGGM TTIPLLVLLG MDDSGSKGDG RHAWTMKHFK KPTYCNFCHI MLMGVRKQGL 310 320 330 340 350 360 CCTYCKYTVH ERCVSRNIPG CVKTYSKAKR SGEVMQHAWV EGNSSVKCDR CHKSIKCYQS 370 380 390 400 410 420 VTARHCVWCR MTFHRKCELS TLCDGGELRD HILLPTSICP ITRDRPGEKS DGCVSAKGEL 430 440 450 460 470 480 VMQYKIIPTP GTHPLLVLVN PKSGGRQGER LNFFRDTPDF RVLACGGDGT VGWILDCIDK 490 500 510 520 530 540 ANFAKHPPVA VLPLGTGNDL ARCLRWGGGY EGGSLTKILK DIEQSPLVML DRWHLEVIPR 550 560 570 580 590 600 EEVENGDQVP YSIMNNYFSI GVDASIAHRF HVMREKHPEK FNSRMKNKLW YFEFGTSETF 610 620 630 640 650 660 AATCKKLHDH IELECDGVGV DLSNIFLEGI AILNIPSMYG GTNLWGENKK NRAVIRESRK 670 680 690 700 710 720 GVTDPKELKF CVQDLSDQLL EVVGLEGAME MGQIYTGLKS AGRRLAQCAS VTIRTNKLLP 730 740 750 760 MQVDGEPWMQ PCCTIKITHK NQAPMMMGPP QKSSFFSLRR KSRSKD
	Isoform 3 [UniParc]. Checksum: 4B58648DC4E785ED	FASTA	752	84,573
	10 20 30 40 50 60 MGEERWVSLT PEEFDQLQKY SEYSSKKIKD ALTEFNEGGS LKQYDPHEPI SYDVFKLFMR 70 80 90 100 110 120 AYLEVDLPQP LSTHLFLAFS QKPRHETSDH PTEGASNSEA NSADTNIQNA DNATKADEAC 130 140 150 160 170 180 APDTESNMAE KQAPAEDQVA ATPLEPPVPR SSSSESPVVY LKDVVCYLSL LETGRPQDKL 190 200 210 220 230 240 EFMFRLYDSD ENGLLDQAEM DCIVNQMLHI AQYLEWDPTE LRPILKEMLQ GMDYDRDGFV 250 260 270 280 290 300 SLQEWVHGGM TTIPLLVLLG MDDSGSKGDG RHAWTMKHFK KPTYCNFCHI MLMGVRKQGL 310 320 330 340 350 360 CCTYCKYTVH ERCVSRNIPG CVKTYSKAKR SGEFHRKCEL STLCDGGELR DHILLPTSIC 370 380 390 400 410 420 PITRDRPGEK SDGCVSAKGE LVMQYKIIPT PGTHPLLVLV NPKSGGRQGE RILRKFHYLL 430 440 450 460 470 480 NPKQVFNLDN GGPTPGLNFF RDTPDFRVLA CGGDGTVGWI LDCIDKANFA KHPPVAVLPL 490 500 510 520 530 540 GTGNDLARCL RWGGGYEGGS LTKILKDIEQ SPLVMLDRWH LEVIPREEVE NGDQVPYSIM 550 560 570 580 590 600 NNYFSIGVDA SIAHRFHVMR EKHPEKFNSR MKNKLWYFEF GTSETFAATC KKLHDHIELE 610 620 630 640 650 660 CDGVGVDLSN IFLEGIAILN IPSMYGGTNL WGENKKNRAV IRESRKGVTD PKELKFCVQD 670 680 690 700 710 720 LSDQLLEVVG LEGAMEMGQI YTGLKSAGRR LAQCASVTIR TNKLLPMQVD GEPWMQPCCT 730 740 750 IKITHKNQAP MMMGPPQKSS FFSLRRKSRS KD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells." Kai M., Sakane F., Imai S., Wada I., Kanoh H. J. Biol. Chem. 269:18492-18498(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT LYS-316. Tissue: Liver.
[2]	"Mapping and genomic characterization of the gene encoding diacylglycerol kinase gamma (DAGK3): assessment of its role in dominant optic atrophy (OPA1)." Stoehr H., Klein J., Gehrig A., Koehler M.R., Jurklies B., Kellner U., Leo-Kottler B., Schmid M., Weber B.H.F. Hum. Genet. 104:99-105(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LYS-316.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-142 AND LYS-316. Tissue: Thalamus.
[4]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), VARIANTS SER-142 AND LYS-316. Tissue: Lymph.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] LYS-706.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26135 mRNA. Translation: BAA05132.1. AF020945 , AF020922, AF020923, AF020924, AF020925, AF020926, AF020927, AF020928, AF020929, AF020930, AF020931, AF020932, AF020933, AF020934, AF020935, AF020936, AF020937, AF020938, AF020939, AF020940, AF020941, AF020942, AF020943, AF020944 Genomic DNA. Translation: AAC04686.1. AK314192 mRNA. Translation: BAG36871.1. AC007917 Genomic DNA. No translation available. AC112649 Genomic DNA. No translation available. BC089411 mRNA. Translation: AAH89411.1. BC112363 mRNA. Translation: AAI12364.1.
IPI	IPI00027658. IPI00216968. IPI00555984.
PIR	A53691.
RefSeq	NP_001074213.1. NM_001080744.1. NP_001074214.1. NM_001080745.1. NP_001337.2. NM_001346.2.
UniGene	Hs.683449.
3D structure databases
ProteinModelPortal	P49619.
SMR	P49619. Positions 1-79, 138-248, 265-323, 333-383.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49619. 2 interactions.
STRING	9606.ENSP00000265022.
PTM databases
PhosphoSite	P49619.
Polymorphism databases
DMDM	311033457.
Proteomic databases
PaxDb	P49619.
PRIDE	P49619.
Protocols and materials databases
DNASU	1608.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265022; ENSP00000265022; ENSG00000058866. ENST00000344484; ENSP00000339777; ENSG00000058866. ENST00000382164; ENSP00000371599; ENSG00000058866.
GeneID	1608.
KEGG	hsa:1608.
UCSC	uc003fqa.3. human. uc003fqb.3. human. uc003fqc.3. human.
Organism-specific databases
CTD	1608.
GeneCards	GC03M185823.
HGNC	HGNC:2853. DGKG.
HPA	HPA036577.
MIM	601854. gene.
neXtProt	NX_P49619.
PharmGKB	PA27314.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG47311.
HOGENOM	HOG000252931.
HOVERGEN	HBG051345.
InParanoid	P49619.
KO	K00901.
OMA	CYQSVTA.
OrthoDB	EOG4GHZNH.
PhylomeDB	P49619.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_604. Hemostasis.
SABIO-RK	P49619.
Gene expression databases
ArrayExpress	P49619.
Bgee	P49619.
CleanEx	HS_DGKG.
Genevestigator	P49619.
GermOnline	ENSG00000058866. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. [Graphical view]
Pfam	PF00130. C1_1. 1 hit. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF13499. EF_hand_5. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1075157.
DrugBank	DB00144. Phosphatidylserine.
GenomeRNAi	1608.
NextBio	6606.
SOURCE	Search...

Entry information

Entry name

DGKG_HUMAN

Accession

Primary (citable) accession number: P49619
Secondary accession number(s): B2RAH4, Q2M1H4, Q5FWG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 2, 2010
Last modified:	May 1, 2013
This is version 132 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.