Reviewed, UniProtKB/Swiss-Prot P49615 (CDK5_MOUSE)

Names and origin

Protein names

Recommended name:
    Cell division protein kinase 5
    EC=2.7.11.22
Alternative name(s):
    Cyclin-dependent kinase 5
    Tau protein kinase II catalytic subunit
      Short name=TPKII catalytic subunit
    Serine/threonine-protein kinase PSSALRE
    CRK6

Gene names

Name:	Cdk5
Synonyms:	Crk6

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with AATK By similarity. Heterodimer of a catalytic subunit and a regulatory subunit (p35). Found in a trimolecular complex with CABLES1 and ABL1. Interacts with CABLES1.
Subcellular location	Cytoplasm. Cell projection › growth cone. Cell projection › lamellipodium. Note: In axonal growth cone with extension to the peripheral lamellipodia.
Tissue specificity	Specifically expressed in post-mitotic neurons.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cell projection Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	Schwann cell development Inferred from mutant phenotype. Source: MGI associative learning Inferred from mutant phenotype. Source: MGI behavioral response to cocaine Inferred from mutant phenotype. Source: MGI cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell-matrix adhesion Inferred from direct assay. Source: MGI central nervous system neuron development Inferred from mutant phenotype. Source: MGI cerebellar cortex formation Inferred from mutant phenotype. Source: MGI corpus callosum development Inferred from mutant phenotype. Source: MGI dendrite morphogenesis Inferred from mutant phenotype. Source: MGI embryonic development Inferred from sequence or structural similarity. Source: UniProtKB hippocampus development Inferred from mutant phenotype. Source: MGI intracellular protein transport Inferred from mutant phenotype. Source: MGI layer formation in the cerebral cortex Inferred from mutant phenotype. Source: MGI motor axon guidance Inferred from mutant phenotype. Source: MGI negative regulation of cell cycle Inferred from mutant phenotype. Source: MGI negative regulation of protein export from nucleus Inferred from mutant phenotype. Source: MGI negative regulation of protein ubiquitination Inferred from mutant phenotype. Source: MGI negative regulation of synaptic plasticity Inferred from mutant phenotype. Source: MGI neuron apoptosis Inferred from mutant phenotype. Source: MGI neuron migration Inferred from mutant phenotype. Source: MGI positive regulation of calcium ion-dependent exocytosis Inferred from direct assay. Source: MGI positive regulation of neuron apoptosis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein kinase activity Inferred from mutant phenotype. Source: MGI receptor catabolic process Inferred from mutant phenotype. Source: MGI receptor clustering Inferred from mutant phenotype. Source: MGI regulation of cell migration Inferred from mutant phenotype. Source: MGI regulation of excitatory postsynaptic membrane potential Inferred from mutant phenotype. Source: MGI sensory perception of pain Inferred from mutant phenotype. Source: MGI serine phosphorylation of STAT3 protein Inferred from direct assay. Source: MGI skeletal muscle tissue development Inferred from direct assay. Source: MGI synaptic transmission, dopaminergic Inferred from mutant phenotype. Source: MGI synaptic transmission, glutamatergic Inferred from mutant phenotype. Source: MGI visual learning Inferred from mutant phenotype. Source: MGI
Cellular component	axon Inferred from sequence or structural similarity. Source: UniProtKB cell soma Inferred from sequence or structural similarity. Source: UniProtKB cytosol Inferred from direct assay. Source: MGI dendrite Inferred from sequence or structural similarity. Source: UniProtKB filopodium Inferred from direct assay. Source: MGI growth cone Inferred from sequence or structural similarity. Source: UniProtKB lamellipodium Inferred from direct assay. Source: MGI membrane Inferred from sequence or structural similarity. Source: UniProtKB neuromuscular junction Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ErbB-2 class receptor binding Inferred from direct assay. Source: UniProtKB ErbB-3 class receptor binding Inferred from direct assay. Source: UniProtKB acetylcholine receptor activator activity Inferred from direct assay. Source: UniProtKB cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC p53 binding Inferred from physical interaction. Source: MGI tau-protein kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Cell division protein kinase 5		PRO_0000085785
Regions
	Domain	4 – 286	283	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	126	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine; by ABL1 Ref.5
	Modified residue	17	1	Phosphothreonine By similarity
	Modified residue	72	1	Phosphoserine By similarity
	Modified residue	159	1	Phosphoserine By similarity
Experimental info
	Mutagenesis	15	1	Y → F: Loss of thyrosine phosphorylations by CABLES1 and ABL1; decreased activity. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P49615-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 4CB11CED9017D535	FASTA	292	33,288
	10 20 30 40 50 60 MQKYEKLEKI GEGTYGTVFK AKNRETHEIV ALKRVRLDDD DEGVPSSALR EICLLKELKH 70 80 90 100 110 120 KNIVRLHDVL HSDKKLTLVF EFCDQDLKKY FDSCNGDLDP EIVKSFLFQL LKGLGFCHSR 130 140 150 160 170 180 NVLHRDLKPQ NLLINRNGEL KLADFGLARA FGIPVRCYSA EVVTLWYRPP DVLFGAKLYS 190 200 210 220 230 240 TSIDMWSAGC IFAELANAGR PLFPGNDVDD QLKRIFRLLG TPTEEQWPAM TKLPDYKPYP 250 260 270 280 290 MYPATTSLVN VVPKLNATGR DLLQNLLKCN PVQRISAEEA LQHPYFSDFC PP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of CDK5 (PSSALRE kinase), a neural cdc2-related protein kinase, in the mature and developing mouse central and peripheral nervous systems." Ino H., Ishizuka T., Chiba T., Tatibana M. Brain Res. 661:196-206(1994) [PubMed: 7834371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"Novel CDC2-related protein kinases produced in murine hematopoietic stem cells." Ershler M.A., Nagorskaya T.V., Visser J.W.M., Belyavsky A.V. Gene 124:305-306(1993) [PubMed: 8444355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 130-165. Strain: CBA. Tissue: Bone marrow.
[4]	"Molecular cloning and chromosomal mapping of the mouse cyclin-dependent kinase 5 gene." Ohshima T., Nagle J.W., Pant H.C., Joshi J.B., Kozak C.A., Brady R.O., Kulkarni A.B. Genomics 28:585-588(1995) [PubMed: 7490100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-12.
[5]	"Cables links Cdk5 and c-Abl and facilitates Cdk5 tyrosine phosphorylation, kinase upregulation, and neurite outgrowth." Zukerberg L.R., Patrick G.N., Nikolic M., Humbert S., Wu C.-L., Lanier L.M., Gertler F.B., Vidal M., Van Etten R.A., Tsai L.-H. Neuron 26:633-646(2000) [PubMed: 10896159] [Abstract] Cited for: IDENTIFICATION IN A TRIMOLECULAR COMPLEX WITH CABLES1 AND ABL1, INTERACTION WITH CABLES1, PHOSPHORYLATION AT TYR-15, MUTAGENESIS OF TYR-15.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D29678 mRNA. Translation: BAA06148.1. BC052007 mRNA. Translation: AAH52007.1. X64604 mRNA. Translation: CAA45888.1. S80121 Genomic DNA. No translation available.
IPI	IPI00309262.
PIR	I49592.
RefSeq	NP_031694.1.
UniGene	Mm.298798
3D structure databases
HSSP	HSSP built from PDB template 1H0V based on UniProtKB P24941.
SMR	P49615. Positions 1-292.
ModBase	Search...
PTM databases
PhosphoSite	P49615.
Genome annotation databases
Ensembl	ENSMUSG00000028969. Mus musculus. [Contig view]
GeneID	12568.
KEGG	mmu:12568.
Organism-specific databases
MGI	MGI:101765. Cdk5.
Phylogenomic databases
HOGENOM	P49615.
HOVERGEN	P49615.
OMA	P49615. RCFSAEV.
Enzyme and pathway databases
BRENDA	2.7.11.22. 244.
Gene expression databases
ArrayExpress	P49615.
Bgee	P49615.
CleanEx	MM_CDK5.
GermOnline	ENSMUSG00000028969. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	281666.
SOURCE	Search...

Entry information

Entry name

CDK5_MOUSE

Accession

Primary (citable) accession number: P49615

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	July 7, 2009
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)