Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P49610

- STRH_STRPN

UniProt

P49610 - STRH_STRPN

Protein

Beta-N-acetylhexosaminidase

Gene

strH

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (26 Sep 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

    GO - Molecular functioni

    1. beta-N-acetylhexosaminidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciSPNE170187:GHGN-63-MONOMER.

    Protein family/group databases

    CAZyiGH20. Glycoside Hydrolase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-N-acetylhexosaminidase (EC:3.2.1.52)
    Gene namesi
    Name:strH
    Ordered Locus Names:SP_0057
    OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
    Taxonomic identifieri170187 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000585: Chromosome

    Subcellular locationi

    Secretedcell wall PROSITE-ProRule annotation; Peptidoglycan-anchor PROSITE-ProRule annotation

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-KW
    3. membrane Source: InterPro

    Keywords - Cellular componenti

    Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence AnalysisAdd
    BLAST
    Chaini34 – 12841251Beta-N-acetylhexosaminidasePRO_0000012020Add
    BLAST
    Propeptidei1285 – 131228Removed by sortasePROSITE-ProRule annotationPRO_0000012021Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1284 – 12841Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation

    Keywords - PTMi

    Peptidoglycan-anchor

    Interactioni

    Protein-protein interaction databases

    STRINGi170187.SP_0057.

    Structurei

    Secondary structure

    1
    1312
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi181 – 1844
    Beta strandi186 – 1938
    Turni194 – 1963
    Helixi201 – 21414
    Beta strandi218 – 22811
    Beta strandi237 – 2393
    Beta strandi242 – 2443
    Helixi246 – 26015
    Helixi271 – 28313
    Beta strandi287 – 29711
    Helixi299 – 30810
    Beta strandi314 – 3174
    Beta strandi320 – 3267
    Helixi331 – 34818
    Turni349 – 3513
    Beta strandi353 – 3586
    Turni362 – 3687
    Helixi371 – 38010
    Beta strandi383 – 3853
    Helixi390 – 40718
    Beta strandi411 – 4166
    Turni417 – 4204
    Helixi421 – 4233
    Beta strandi435 – 4384
    Helixi451 – 4566
    Beta strandi461 – 4633
    Helixi466 – 4683
    Helixi484 – 49310
    Beta strandi510 – 5178
    Helixi527 – 54014
    Turni541 – 5444
    Helixi550 – 5578
    Helixi568 – 58215
    Helixi590 – 5923
    Helixi593 – 60816
    Helixi627 – 6304
    Beta strandi631 – 6388
    Turni639 – 6413
    Helixi646 – 65914
    Beta strandi663 – 67311
    Beta strandi682 – 6843
    Beta strandi687 – 6893
    Helixi691 – 70515
    Helixi716 – 72813
    Beta strandi732 – 74211
    Helixi744 – 7529
    Beta strandi759 – 77012
    Helixi775 – 79218
    Turni793 – 7953
    Beta strandi796 – 8027
    Helixi808 – 8103
    Helixi815 – 8217
    Helixi825 – 84117
    Beta strandi845 – 8506
    Turni851 – 8544
    Helixi855 – 8573
    Beta strandi868 – 8714
    Helixi884 – 8896
    Beta strandi893 – 8964
    Helixi899 – 9013
    Beta strandi902 – 9043
    Helixi909 – 9113
    Helixi916 – 92510
    Turni936 – 9383
    Beta strandi943 – 9519
    Helixi961 – 97414
    Turni975 – 9784
    Helixi984 – 9907
    Helixi1002 – 101312
    Helixi1021 – 10233
    Helixi1024 – 103613
    Beta strandi1055 – 10584
    Beta strandi1060 – 10634
    Beta strandi1065 – 10739
    Beta strandi1085 – 10884
    Beta strandi1092 – 10943
    Beta strandi1099 – 110911
    Beta strandi1118 – 11203
    Beta strandi1131 – 11344

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LTJNMR-A1050-1140[»]
    2YL5X-ray2.15A/B/C/D627-1064[»]
    2YL6X-ray1.60A181-614[»]
    2YL8X-ray1.75A181-614[»]
    2YL9X-ray2.65A/B/C/D627-1062[»]
    2YLAX-ray2.70A/B/C/D627-1064[»]
    2YLLX-ray1.85A181-614[»]
    4AZ5X-ray1.73A181-614[»]
    4AZ6X-ray1.36A181-613[»]
    4AZ7X-ray1.70A181-613[»]
    4AZBX-ray2.10A181-614[»]
    4AZCX-ray2.09A/B/C/D627-1064[»]
    4AZGX-ray2.40A/B627-1064[»]
    4AZHX-ray2.22A/B/C/D627-1064[»]
    4AZIX-ray1.98A/B627-1064[»]
    ProteinModelPortaliP49610.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1059 – 113880G5 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1150 – 123081G5 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni176 – 616441Catalytic domain 1Add
    BLAST
    Regioni621 – 1046426Catalytic domain 2Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1281 – 12855LPXTG sorting signalPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 20 family.Curated
    Contains 2 G5 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG3525.
    HOGENOMiHOG000285052.
    KOiK12373.
    OMAiTYASDDV.
    OrthoDBiEOG6GFGKT.

    Family and domain databases

    Gene3Di3.20.20.80. 2 hits.
    InterProiIPR011098. G5_dom.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR019948. Gram-positive_anchor.
    IPR019931. LPXTG_anchor.
    IPR005877. YSIRK_signal_dom.
    [Graphical view]
    PfamiPF07501. G5. 2 hits.
    PF00728. Glyco_hydro_20. 2 hits.
    PF00746. Gram_pos_anchor. 1 hit.
    PF04650. YSIRK_signal. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 2 hits.
    TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
    TIGR01168. YSIRK_signal. 1 hit.
    PROSITEiPS51109. G5. 2 hits.
    PS50847. GRAM_POS_ANCHORING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49610-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKHEKQQRFS IRKYAVGAAS VLIGFAFQAQ TVAADGVTPT TTENQPTIHT     50
    VSDSPQSSEN RTEETPKAVL QPEAPKTVET ETPATDKVAS LPKTEEKPQE 100
    EVSSTPSDKA EVVTPTSAEK ETANKKAEEA SPKKEEAKEV DSKESNTDKT 150
    DKDKPAKKDE AKAEADKPAT EAGKERAATV NEKLAKKKIV SIDAGRKYFS 200
    PEQLKEIIDK AKHYGYTDLH LLVGNDGLRF MLDDMSITAN GKTYASDDVK 250
    RAIEKGTNDY YNDPNGNHLT ESQMTDLINY AKDKGIGLIP TVNSPGHMDA 300
    ILNAMKELGI QNPNFSYFGK KSARTVDLDN EQAVAFTKAL IDKYAAYFAK 350
    KTEIFNIGLD EYANDATDAK GWSVLQADKY YPNEGYPVKG YEKFIAYAND 400
    LARIVKSHGL KPMAFNDGIY YNSDTSFGSF DKDIIVSMWT GGWGGYDVAS 450
    SKLLAEKGHQ ILNTNDAWYY VLGRNADGQG WYNLDQGLNG IKNTPITSVP 500
    KTEGADIPII GGMVAAWADT PSARYSPSRL FKLMRHFANA NAEYFAADYE 550
    SAEQALNEVP KDLNRYTAES VTAVKEAEKA IRSLDSNLSR AQQDTIDQAI 600
    AKLQETVNNL TLTPEAQKEE EAKREVEKLA KNKVISIDAG RKYFTLNQLK 650
    RIVDKASELG YSDVHLLLGN DGLRFLLDDM TITANGKTYA SDDVKKAIIE 700
    GTKAYYDDPN GTALTQAEVT ELIEYAKSKD IGLIPAINSP GHMDAMLVAM 750
    EKLGIKNPQA HFDKVSKTTM DLKNEEAMNF VKALIGKYMD FFAGKTKIFN 800
    FGTDEYANDA TSAQGWYYLK WYQLYGKFAE YANTLAAMAK ERGLQPMAFN 850
    DGFYYEDKDD VQFDKDVLIS YWSKGWWGYN LASPQYLASK GYKFLNTNGD 900
    WYYILGQKPE DGGGFLKKAI ENTGKTPFNQ LASTKYPEVD LPTVGSMLSI 950
    WADRPSAEYK EEEIFELMTA FADHNKDYFR ANYNALREEL AKIPTNLEGY 1000
    SKESLEALDA AKTALNYNLN RNKQAELDTL VANLKAALQG LKPAVTHSGS 1050
    LDENEVAANV ETRPELITRT EEIPFEVIKK ENPNLPAGQE NIITAGVKGE 1100
    RTHYISVLTE NGKTTETVLD SQVTKEVINQ VVEVGAPVTH KGDESGLAPT 1150
    TEVKPRLDIQ EEEIPFTTVT CENPLLLKGK TQVITKGVNG HRSNFYSVST 1200
    SADGKEVKTL VNSVVAQEAV TQIVEVGTMV THVGDENGQA AIAEEKPKLE 1250
    IPSQPAPSTA PAEESKVLPQ DPAPVVTEKK LPETGTHDSA GLVVAGLMST 1300
    LAAYGLTKRK ED 1312
    Length:1,312
    Mass (Da):144,550
    Last modified:September 26, 2001 - v2
    Checksum:i503375B5257A90B5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti39 – 391Missing in AAC41450. (PubMed:7721787)Curated
    Sequence conflicti69 – 691V → E in AAC41450. (PubMed:7721787)Curated
    Sequence conflicti169 – 1691A → E in AAC41450. (PubMed:7721787)Curated
    Sequence conflicti617 – 6171Q → L in AAC41450. (PubMed:7721787)Curated
    Sequence conflicti1045 – 10451V → A in AAC41450. (PubMed:7721787)Curated
    Sequence conflicti1161 – 11611E → K in AAC41450. (PubMed:7721787)Curated
    Sequence conflicti1171 – 11711C → R in AAC41450. (PubMed:7721787)Curated
    Sequence conflicti1267 – 12671V → A in AAC41450. (PubMed:7721787)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36923 Genomic DNA. Translation: AAC41450.1.
    AE005672 Genomic DNA. Translation: AAK74246.1.
    PIRiA56390.
    E95006.
    RefSeqiNP_344606.1. NC_003028.3.

    Genome annotation databases

    EnsemblBacteriaiAAK74246; AAK74246; SP_0057.
    GeneIDi929812.
    KEGGispn:SP_0057.
    PATRICi19704441. VBIStrPne105772_0065.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36923 Genomic DNA. Translation: AAC41450.1 .
    AE005672 Genomic DNA. Translation: AAK74246.1 .
    PIRi A56390.
    E95006.
    RefSeqi NP_344606.1. NC_003028.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LTJ NMR - A 1050-1140 [» ]
    2YL5 X-ray 2.15 A/B/C/D 627-1064 [» ]
    2YL6 X-ray 1.60 A 181-614 [» ]
    2YL8 X-ray 1.75 A 181-614 [» ]
    2YL9 X-ray 2.65 A/B/C/D 627-1062 [» ]
    2YLA X-ray 2.70 A/B/C/D 627-1064 [» ]
    2YLL X-ray 1.85 A 181-614 [» ]
    4AZ5 X-ray 1.73 A 181-614 [» ]
    4AZ6 X-ray 1.36 A 181-613 [» ]
    4AZ7 X-ray 1.70 A 181-613 [» ]
    4AZB X-ray 2.10 A 181-614 [» ]
    4AZC X-ray 2.09 A/B/C/D 627-1064 [» ]
    4AZG X-ray 2.40 A/B 627-1064 [» ]
    4AZH X-ray 2.22 A/B/C/D 627-1064 [» ]
    4AZI X-ray 1.98 A/B 627-1064 [» ]
    ProteinModelPortali P49610.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 170187.SP_0057.

    Protein family/group databases

    CAZyi GH20. Glycoside Hydrolase Family 20.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK74246 ; AAK74246 ; SP_0057 .
    GeneIDi 929812.
    KEGGi spn:SP_0057.
    PATRICi 19704441. VBIStrPne105772_0065.

    Phylogenomic databases

    eggNOGi COG3525.
    HOGENOMi HOG000285052.
    KOi K12373.
    OMAi TYASDDV.
    OrthoDBi EOG6GFGKT.

    Enzyme and pathway databases

    BioCyci SPNE170187:GHGN-63-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 2 hits.
    InterProi IPR011098. G5_dom.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR019948. Gram-positive_anchor.
    IPR019931. LPXTG_anchor.
    IPR005877. YSIRK_signal_dom.
    [Graphical view ]
    Pfami PF07501. G5. 2 hits.
    PF00728. Glyco_hydro_20. 2 hits.
    PF00746. Gram_pos_anchor. 1 hit.
    PF04650. YSIRK_signal. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 2 hits.
    TIGRFAMsi TIGR01167. LPXTG_anchor. 1 hit.
    TIGR01168. YSIRK_signal. 1 hit.
    PROSITEi PS51109. G5. 2 hits.
    PS50847. GRAM_POS_ANCHORING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the beta-N-acetylglucosaminidase gene from Streptococcus pneumoniae. Generation of truncated enzymes with modified aglycon specificity."
      Clarke V.A., Platt N., Butters T.D.
      J. Biol. Chem. 270:8805-8814(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 12213.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-334 / TIGR4.

    Entry informationi

    Entry nameiSTRH_STRPN
    AccessioniPrimary (citable) accession number: P49610
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3