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Reviewed, UniProtKB/Swiss-Prot P49608 (ACOC_CUCMA)

Last modified June 16, 2009. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Aconitate hydratase, cytoplasmic
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase
OrganismCucurbita maxima (Pumpkin) (Winter squash)
Taxonomic identifier3661 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids ICucurbitalesCucurbitaceaeCucurbita

Protein attributes

Sequence length898 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

Citrate = isocitrate.

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Carbohydrate metabolism; glyoxylate and dicarboxylate metabolism.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
   Molecular functionLyase
Gene Ontology (GO)
   Biological processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

aconitate hydratase activity

Inferred from electronic annotation. Source: EC

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 898898Aconitate hydratase, cytoplasmic
PRO_0000076652

Sites

Metal binding4411Iron-sulfur (4Fe-4S) By similarity
Metal binding5071Iron-sulfur (4Fe-4S) By similarity
Metal binding5101Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P49608-1 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: ADFE73173CA54971

FASTA89898,005
        10         20         30         40         50         60 
MAAENPFKEN LTSLPKPGGG EFGKYYSLPS LNDPRIDRLP YSIRILLESA IRNCDNFQVK 

        70         80         90        100        110        120 
KEDVEKIIDW ENSSPKQVEI PFKPARVLLQ DFTGVPAVVD LACMRDAMNK LGSDSNKINP 

       130        140        150        160        170        180 
LVPVDLVIDH SVQVDVARSE NAVQANMELE FQRNKERFAF LKWGSNAFQN MLVVPPGSGI 

       190        200        210        220        230        240 
VHQVNLEYLG RVVFNTSGLL YPDSVVGTDS HTTMIDGLGV AGWGVGGIEA EAAMLGQPMS 

       250        260        270        280        290        300 
MVLPGVVGFK LSGKLRNGVT ATDLVLTVTQ MLRKHGVVGK FVEFYGDGME ELSLADRATI 

       310        320        330        340        350        360 
ANMSPEYGAT MGFFPVDHVT LQYLKLTGRS DETVSMIEAY LRANKMFVDY KEPQQEKVYS 

       370        380        390        400        410        420 
SYLQLDLTDV EPCISGPKRP HDRVPLKEMK SDWHACLDNK VGFKGFAIPK EAQENVAKFS 

       430        440        450        460        470        480 
FHGQPAELKH GSVVIAAITS CTNTSNPSVM LGAALVAKKA CELGLQVKPW VKTSLAPGSG 

       490        500        510        520        530        540 
VVTKYLLKSG LQPYLNQQGF HIVGYGCTTC IGNSGDLDES VSAAISDNDI VAAAVLSGNR 

       550        560        570        580        590        600 
NFEGRVHPLT RANYLASPPL VVAYALAGTV DIDFEKEPIG KGKDGKDVYF RDIWPSTEEI 

       610        620        630        640        650        660 
AEVVQSSVLP DMFKSTYESI TKGNPMWNQL SVPSGTLYSW DPNSTYIHEP PYFKNMTMDP 

       670        680        690        700        710        720 
PGAHGVKDAY CLLNFGDSIT TDHISPAGSI HKDSPAAKYL LERGVDRKDF NSYGSRRGND 

       730        740        750        760        770        780 
EVMARGTFAN IRLVNKLLDG EVGPKTVHVP TGEKLSVFEA AEKYKSAGQD TIVLAGAEYG 

       790        800        810        820        830        840 
SGSSRDWAAK GPMLLGVKAV IAKSFERIHR SNLVGMGIIP LCFKSGEDAD SLGLTGHERY 

       850        860        870        880        890 
TIDLPDDISK IRPGQDVTVT TDSGKSFTCT VRFDTEVELA YFNNGGILPY VIRNLIKQ 

« Hide

References

[1]"Cytosolic aconitase participates in the glyoxylate cycle in etiolated pumpkin cotyledons."
Hayashi M., de Bellis L., Alpi A., Nishimura M.
Plant Cell Physiol. 36:669-680(1995) [PubMed: 7640891] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Kurokawa Amakuri Nankin.

Cross-references

Sequence databases

D29629 mRNA. Translation: BAA06108.1.

3D structure databases

HSSPHSSP built from PDB template 1AMJ based on UniProtKB P20004.
ModBaseSearch...

Enzyme and pathway databases

BRENDA4.2.1.3. 2176.

Family and domain databases

InterProIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015937. Aconitase-like_core.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR006249. Aconitase/Fe_reg_prot_2.
IPR015934. Aconitase/Fe_reg_prot_2/AcnD.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
Gene3DG3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits.
G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit.
G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHERPTHR11670. Aconitase-like_core. 1 hit.
PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
PfamPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSPR00415. ACONITASE.
ProDomPD000511. Aconitase_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01341. aconitase_1. 1 hit.
PROSITEPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACOC_CUCMA
AccessionPrimary (citable) accession number: P49608
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents