Reviewed, UniProtKB/Swiss-Prot P49604 (GATA_USTMA)

Names and origin

Protein names

Recommended name:
    4-aminobutyrate aminotransferase
    EC=2.6.1.19
Alternative name(s):
    Gamma-amino-N-butyrate transaminase
      Short name=GABA transaminase
    GABA aminotransferase
      Short name=GABA-AT

Gene names

Name:	GATA
ORF Names:	UM01080

Organism

Ustilago maydis (Smut fungus) [Complete proteome]

Taxonomic identifier

5270 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Deaminates gamma-aminobutyric acid (GABA) to succinate-semialdehyde, which in turn is converted to succinate by the succinate semialdehyde dehydrogenase By similarity. Not required for the utilization of GABA as nitrogen source.
Catalytic activity	4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Induction	Up-regulated by GABA and beta-alanine. Is not subject to nitrogen catabolite repression. Ref.1
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gamma-aminobutyric acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-aminobutyrate transaminase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	4-aminobutyrate aminotransferase		PRO_0000120381
Amino acid modifications
	Modified residue	363	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	337	1	G → GT in AAA98560. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49604-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: 9ECAFAE83FE4DD4C	FASTA	509	55,993
	10 20 30 40 50 60 MISSKATSAA RACARTSRVM QQQQRRLLAT VVSSSSLFPG EPSSPHVVTS QIPGPKSKEL 70 80 90 100 110 120 SDRIGTFQEN RTHGFVVDYA KSQGNWIADA DGNVLLDMFA QIASIAIGYN NPDLLALAKT 130 140 150 160 170 180 DEFITATMNR AALGSFPPTN WQELVETSFG TVKPDGLNNI FTAMCGSCAN ENAFKASFMA 190 200 210 220 230 240 YRARERGEKA EFTPEEMSSC MKNQSPGSPD LSILSFTSAF HGRLFGSLSA TRSKAIHKLD 250 260 270 280 290 300 IPSFNWPVVE WPDVKYPFAQ NSRENAEAEK VALAAVEEAI VSSKKTGSSY GPVAALIVEP 310 320 330 340 350 360 IQSEGGDNHA SPAFFQGLRD VTKKHGVFMI VDEVQTGVGA TGAFWAHSKW NLTSPPDFVT 370 380 390 400 410 420 FSKKMQAAGF YHNIETRPSL PYRNYNTWMG DPARTLQARQ IIRTIQDHNL IQKTDKVGNY 430 440 450 460 470 480 IYEKLFDLIE NGAGRGKIEK LRGENAGTFL AFDGRTAKVR DQLIMEMRKL GVHMGGCGDK 490 500 ALRLRPMLVF EQKHADIFLD KLETALGQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the ugatA gene of Ustilago maydis, isolated by homology to the gatA gene of Aspergillus nidulans." Straffon M.J., Hynes M.J., Davis M.A. Curr. Genet. 29:360-369(1996) [PubMed: 8598057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION. Strain: 518.
[2]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U28655 Genomic DNA. Translation: AAA98560.1. AACP01000037 Genomic DNA. Translation: EAK82039.1.
PIR	S68116.
RefSeq	XP_757227.1.
3D structure databases
HSSP	HSSP built from PDB template 1GTX based on UniProtKB P80147.
ModBase	Search...
Protein-protein interaction databases
STRING	P49604.
Genome annotation databases
GeneID	3629154.
KEGG	uma:UM01080.1.
Phylogenomic databases
OMA	KERGQRG.
Enzyme and pathway databases
BRENDA	2.6.1.19. 2320.
Family and domain databases
InterPro	IPR004631. 4NH2But_aminotransferase_euk. IPR005814. Aminotrans_3. [Graphical view]
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF6. GABAtrns_euk. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00699. GABAtrns_euk. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATA_USTMA

Accession

Primary (citable) accession number: P49604
Secondary accession number(s): Q4PFN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)