P49602 (CP51_USTMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lanosterol 14-alpha demethylase
EC=1.14.13.70

Alternative name(s):
CYPLI
Cytochrome P450 51
Cytochrome P450-14DM
Cytochrome P450-LIA1
Sterol 14-alpha demethylase

Gene names

Name:	ERG11
Synonyms:	CYP51
ORF Names:	UM03662

Organism

Ustilago maydis (strain 521 / FGSC 9021) (Smut fungus)

Taxonomic identifier

237631 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Membrane Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Lanosterol 14-alpha demethylase		PRO_0000052011
Sites
	Metal binding	501	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49602 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 2F9A6647D9B17FB0	FASTA	561	62,197
	10 20 30 40 50 60 MVASSSSATA SLLDQLFALT PLADSSAWIK TITVLVLLPL LAVVLNVASQ LLLATPKNHP 70 80 90 100 110 120 PVVFHFVPVI GSAIYYGIDP YKFFFECREK YGDVFTFVLL GRKITVALGP KGSNLVFNAK 130 140 150 160 170 180 HQQVTAEDAY THLTTPVFGK EVVYDVPNAV FMEQKKFVKV GLSIENFRVY VPQIVDEVRE 190 200 210 220 230 240 YIKSDARFSA LKTRKTITVD IFQAMSELII LTASRTLQGK EVRQGLDKSF AQLYHDLDSG 250 260 270 280 290 300 FTPINFVIPN LPLPSNFKRD RAQKKMSQFY QDIVAKRRAA GASTSADDAS GENDMIAALI 310 320 330 340 350 360 EQKYKNGRAL SGVEIAHMMI ALLMAGQHTS SATSSWAFLR LASRPEIIEE LYEEQLNVYS 370 380 390 400 410 420 DGHGGLRELD YETQKTSVPL LDAVVKETLR LHPPLHSIMR YVKSDLAVPP TLSSPTSTKS 430 440 450 460 470 480 EPDAHYVIPK GHYIMAAPGV SQVDPQIWKS SDQFDPHRWL DATTAAAMQD SGEDKQDFGF 490 500 510 520 530 540 GMISTGANSP YLPFGAGRHR CIGEQFAYLQ IGVILATFVR IFKWHLDSKF PDPDYQSMVV 550 560 LPSKNGCAIV LTPRAESLHL D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of an Ustilago maydis ERG11 gene and its expression in a mutant deficient in sterol 14 alpha-demethylase activity." Hargreaves J.A., Keon J.P.R. FEMS Microbiol. Lett. 139:203-207(1996) [PubMed: 8674989] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IMI 103761.
[2]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48164 Genomic DNA. Translation: CAA88176.1. AACP01000124 Genomic DNA. Translation: EAK84840.1.
PIR	S52319.
RefSeq	XP_759809.1. XM_754716.1.
3D structure databases
ProteinModelPortal	P49602.
ModBase	Search...
Protein-protein interaction databases
STRING	P49602.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	UM03662T0; UM03662P0; UM03662.
GeneID	3631809.
KEGG	uma:UM03662.1.
Phylogenomic databases
eggNOG	fuNOG06039.
HOGENOM	HBG566517.
OMA	ATYKDGR.
OrthoDB	EOG4QJVWH.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
KO	K05917.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_USTMA

Accession

Primary (citable) accession number: P49602
Secondary accession number(s): Q4P8A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program