Reviewed, UniProtKB/Swiss-Prot P49602 (CP51_USTMA)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 51
    EC=1.14.13.70
Alternative name(s):
    CYPLI
    P450-LIA1
    Sterol 14-alpha demethylase
    Lanosterol 14-alpha demethylase
    P450-14DM

Gene names

Name:	ERG11
Synonyms:	CYP51
ORF Names:	UM03662

Organism

Ustilago maydis (Smut fungus) [Complete proteome]

Taxonomic identifier

5270 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.
Catalytic activity	Obtusifoliol + 3 O(2) + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP(+) + 4 H(2)O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	MembranePotential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Cytochrome P450 51		PRO_0000052011
Sites
	Metal binding	501	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49602-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 2F9A6647D9B17FB0	FASTA	561	62,197
	10 20 30 40 50 60 MVASSSSATA SLLDQLFALT PLADSSAWIK TITVLVLLPL LAVVLNVASQ LLLATPKNHP 70 80 90 100 110 120 PVVFHFVPVI GSAIYYGIDP YKFFFECREK YGDVFTFVLL GRKITVALGP KGSNLVFNAK 130 140 150 160 170 180 HQQVTAEDAY THLTTPVFGK EVVYDVPNAV FMEQKKFVKV GLSIENFRVY VPQIVDEVRE 190 200 210 220 230 240 YIKSDARFSA LKTRKTITVD IFQAMSELII LTASRTLQGK EVRQGLDKSF AQLYHDLDSG 250 260 270 280 290 300 FTPINFVIPN LPLPSNFKRD RAQKKMSQFY QDIVAKRRAA GASTSADDAS GENDMIAALI 310 320 330 340 350 360 EQKYKNGRAL SGVEIAHMMI ALLMAGQHTS SATSSWAFLR LASRPEIIEE LYEEQLNVYS 370 380 390 400 410 420 DGHGGLRELD YETQKTSVPL LDAVVKETLR LHPPLHSIMR YVKSDLAVPP TLSSPTSTKS 430 440 450 460 470 480 EPDAHYVIPK GHYIMAAPGV SQVDPQIWKS SDQFDPHRWL DATTAAAMQD SGEDKQDFGF 490 500 510 520 530 540 GMISTGANSP YLPFGAGRHR CIGEQFAYLQ IGVILATFVR IFKWHLDSKF PDPDYQSMVV 550 560 LPSKNGCAIV LTPRAESLHL D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of an Ustilago maydis ERG11 gene and its expression in a mutant deficient in sterol 14 alpha-demethylase activity." Hargreaves J.A., Keon J.P.R. FEMS Microbiol. Lett. 139:203-207(1996) [PubMed: 8674989] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IMI 103761.
[2]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z48164 Genomic DNA. Translation: CAA88176.1. AACP01000124 Genomic DNA. Translation: EAK84840.1.
PIR	S52319.
RefSeq	XP_759809.1.
3D structure databases
HSSP	HSSP built from PDB template 1E9X based on UniProtKB P77901.
ModBase	Search...
Genome annotation databases
GeneID	3631809.
KEGG	uma:UM03662.1.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_USTMA

Accession

Primary (citable) accession number: P49602
Secondary accession number(s): Q4P8A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 25, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)