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P49600 (SP2E_BACME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Stage II sporulation protein E

EC=3.1.3.16
Gene names
Name:spoIIE
OrganismBacillus megaterium
Taxonomic identifier1404 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length585 AA.
Sequence statusFragment.
Protein existenceInferred from homology

General annotation (Comments)

Function

Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates SpoIIAA by dephosphorylation By similarity.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Note: Polar septum By similarity.

Sequence similarities

Contains 1 PP2C-like domain.

Ontologies

Keywords
   Biological processSporulation
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionHydrolase
Protein phosphatase
Gene Ontology (GO)
   Biological_processsporulation resulting in formation of a cellular spore

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphosphoprotein phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 585›585Stage II sporulation protein E
PRO_0000057793

Regions

Transmembrane40 – 5718Helical; Potential
Transmembrane70 – 8617Helical; Potential
Domain366 – 585220PP2C-like

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P49600 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 90E9ACF1D3E21D01

FASTA58565,690
        10         20         30         40         50         60 
TVGVVTGLIL SFANVSSLYE MSLLAFSGLL GGLLKDGKKL GARLGLVVGS LLIGLYAQAD 

        70         80         90        100        110        120 
QGLTTNLYES LTAVVLFLLT PSFVLKNLSK LVPGTSENML EQQQYVRKIR DVTANRVEQF 

       130        140        150        160        170        180 
SNVFQALSKS FTQNGFYDEK PSADKEVDYF LSSVTERTCQ FCFKKEQCWA QQFDTTYEYM 

       190        200        210        220        230        240 
KEIMLEVDNG TLEQNPRLIR EMDKHCVKSK KVIDVIEHEL TYFKANQHLK AQIQESRRIV 

       250        260        270        280        290        300 
AEQLHGVSEV MGNFAKEIKR ERENLNVQEE QILEALRNFG MEINQIEIYS LEPGNVDIEM 

       310        320        330        340        350        360 
WVPYCHGRGE CEKIIAPMLT DILGESIVVK NEECAKYPQG YCHVSFGCTK AYVVDTGVAH 

       370        380        390        400        410        420 
AAKGGGFVSG DSYSMIELNA GKYALAISDG MGNGERAHYE SSETLQLLKQ ILQTGIEETI 

       430        440        450        460        470        480 
AIKSINSILS LRTNDEIFST LDLAMIDLQD ANVNFLKIGS TPSFIKRGDK VIKIQASNLP 

       490        500        510        520        530        540 
MGIIEEFEVD VVNEQMKAED LLIMMSDGVF EGPKHVENYE MWMKRKIGEL QTNDPQEIAD 

       550        560        570        580 
LIMEEVIRTK VGLIEDDMTV VVAKLQHNTP KWSSIPSYAY RNLAQ 

« Hide

References

[1]"Structure and function of the Bacillus SpoIIE protein and its localization to sites of sporulation septum assembly."
Barak I., Behari J., Olmedo G., Guzman P., Brown D.P., Castro E., Walker D., Westpheling J., Youngman P.
Mol. Microbiol. 19:1047-1060(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 35985 / VT1660.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U26836 Genomic DNA. Translation: AAB58072.1.
PIRS71018.
S73325.

3D structure databases

ProteinModelPortalP49600.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR014221. Spore_II_E.
[Graphical view]
PfamPF07228. SpoIIE. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
TIGRFAMsTIGR02865. spore_II_E. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSP2E_BACME
AccessionPrimary (citable) accession number: P49600
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families