P49600 (SP2E_BACME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Stage II sporulation protein E
EC=3.1.3.16

Gene names

Name:

spoIIE

Organism

Bacillus megaterium

Taxonomic identifier

1404 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	585 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates SpoIIAA by dephosphorylation By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Note: Polar septum By similarity.
Sequence similarities	Contains 1 PP2C-like domain.

Ontologies

Keywords
Biological process	Sporulation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 585	›585	Stage II sporulation protein E		PRO_0000057793
Regions
	Transmembrane	40 – 57	18	Helical; Potential
	Transmembrane	70 – 86	17	Helical; Potential
	Domain	366 – 585	220	PP2C-like
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P49600 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 90E9ACF1D3E21D01	FASTA	585	65,690
	10 20 30 40 50 60 TVGVVTGLIL SFANVSSLYE MSLLAFSGLL GGLLKDGKKL GARLGLVVGS LLIGLYAQAD 70 80 90 100 110 120 QGLTTNLYES LTAVVLFLLT PSFVLKNLSK LVPGTSENML EQQQYVRKIR DVTANRVEQF 130 140 150 160 170 180 SNVFQALSKS FTQNGFYDEK PSADKEVDYF LSSVTERTCQ FCFKKEQCWA QQFDTTYEYM 190 200 210 220 230 240 KEIMLEVDNG TLEQNPRLIR EMDKHCVKSK KVIDVIEHEL TYFKANQHLK AQIQESRRIV 250 260 270 280 290 300 AEQLHGVSEV MGNFAKEIKR ERENLNVQEE QILEALRNFG MEINQIEIYS LEPGNVDIEM 310 320 330 340 350 360 WVPYCHGRGE CEKIIAPMLT DILGESIVVK NEECAKYPQG YCHVSFGCTK AYVVDTGVAH 370 380 390 400 410 420 AAKGGGFVSG DSYSMIELNA GKYALAISDG MGNGERAHYE SSETLQLLKQ ILQTGIEETI 430 440 450 460 470 480 AIKSINSILS LRTNDEIFST LDLAMIDLQD ANVNFLKIGS TPSFIKRGDK VIKIQASNLP 490 500 510 520 530 540 MGIIEEFEVD VVNEQMKAED LLIMMSDGVF EGPKHVENYE MWMKRKIGEL QTNDPQEIAD 550 560 570 580 LIMEEVIRTK VGLIEDDMTV VVAKLQHNTP KWSSIPSYAY RNLAQ

References

[1]

"Structure and function of the Bacillus SpoIIE protein and its localization to sites of sporulation septum assembly." Barak I., Behari J., Olmedo G., Guzman P., Brown D.P., Castro E., Walker D., Westpheling J., Youngman P. Mol. Microbiol. 19:1047-1060(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35985 / VT1660.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U26836 Genomic DNA. Translation: AAB58072.1.
PIR	S71018. S73325.
3D structure databases
ProteinModelPortal	P49600.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR014221. Spore_II_E. [Graphical view]
Pfam	PF07228. SpoIIE. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
TIGRFAMs	TIGR02865. spore_II_E. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SP2E_BACME

Accession

Primary (citable) accession number: P49600

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program