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Protein

Stage II sporulation protein E

Gene

spoIIE

Organism
Bacillus megaterium
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates SpoIIAA by dephosphorylation (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

  1. phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  1. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Sporulation

Names & Taxonomyi

Protein namesi
Recommended name:
Stage II sporulation protein E (EC:3.1.3.16)
Gene namesi
Name:spoIIE
OrganismiBacillus megaterium
Taxonomic identifieri1404 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Polar septum.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei40 – 5718HelicalSequence AnalysisAdd
BLAST
Transmembranei70 – 8617HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 585›585Stage II sporulation protein EPRO_0000057793Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP49600.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini366 – 585220PP2C-likeAdd
BLAST

Sequence similaritiesi

Contains 1 PP2C-like domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR014221. Spore_II_E.
[Graphical view]
PfamiPF07228. SpoIIE. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
TIGRFAMsiTIGR02865. spore_II_E. 1 hit.

Sequencei

Sequence statusi: Fragment.

P49600-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
TVGVVTGLIL SFANVSSLYE MSLLAFSGLL GGLLKDGKKL GARLGLVVGS
60 70 80 90 100
LLIGLYAQAD QGLTTNLYES LTAVVLFLLT PSFVLKNLSK LVPGTSENML
110 120 130 140 150
EQQQYVRKIR DVTANRVEQF SNVFQALSKS FTQNGFYDEK PSADKEVDYF
160 170 180 190 200
LSSVTERTCQ FCFKKEQCWA QQFDTTYEYM KEIMLEVDNG TLEQNPRLIR
210 220 230 240 250
EMDKHCVKSK KVIDVIEHEL TYFKANQHLK AQIQESRRIV AEQLHGVSEV
260 270 280 290 300
MGNFAKEIKR ERENLNVQEE QILEALRNFG MEINQIEIYS LEPGNVDIEM
310 320 330 340 350
WVPYCHGRGE CEKIIAPMLT DILGESIVVK NEECAKYPQG YCHVSFGCTK
360 370 380 390 400
AYVVDTGVAH AAKGGGFVSG DSYSMIELNA GKYALAISDG MGNGERAHYE
410 420 430 440 450
SSETLQLLKQ ILQTGIEETI AIKSINSILS LRTNDEIFST LDLAMIDLQD
460 470 480 490 500
ANVNFLKIGS TPSFIKRGDK VIKIQASNLP MGIIEEFEVD VVNEQMKAED
510 520 530 540 550
LLIMMSDGVF EGPKHVENYE MWMKRKIGEL QTNDPQEIAD LIMEEVIRTK
560 570 580
VGLIEDDMTV VVAKLQHNTP KWSSIPSYAY RNLAQ
Length:585
Mass (Da):65,690
Last modified:February 1, 1996 - v1
Checksum:i90E9ACF1D3E21D01
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26836 Genomic DNA. Translation: AAB58072.1.
PIRiS71018.
S73325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26836 Genomic DNA. Translation: AAB58072.1.
PIRiS71018.
S73325.

3D structure databases

ProteinModelPortaliP49600.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR014221. Spore_II_E.
[Graphical view]
PfamiPF07228. SpoIIE. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
TIGRFAMsiTIGR02865. spore_II_E. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Structure and function of the Bacillus SpoIIE protein and its localization to sites of sporulation septum assembly."
    Barak I., Behari J., Olmedo G., Guzman P., Brown D.P., Castro E., Walker D., Westpheling J., Youngman P.
    Mol. Microbiol. 19:1047-1060(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 35985 / VT1660.

Entry informationi

Entry nameiSP2E_BACME
AccessioniPrimary (citable) accession number: P49600
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: January 7, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.