Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein phosphatase 2C 57

Gene

PPH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi93Manganese 1By similarity1
Metal bindingi93Manganese 2By similarity1
Metal bindingi94Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi296Manganese 2By similarity1
Metal bindingi339Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phosphatase activity Source: TAIR
  • phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  • dephosphorylation Source: TAIR
  • photosynthetic electron transport chain Source: TAIR
  • photosystem stoichiometry adjustment Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 57 (EC:3.1.3.16)
Short name:
AtPP2C57
Alternative name(s):
Protein phosphatase 2C PPH1
Short name:
PP2C PPH1
Gene namesi
Name:PPH1
Ordered Locus Names:At4g27800
ORF Names:T27E11.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G27800.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei363 – 383HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytoplasm Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • nucleolus Source: TAIR
  • nucleus Source: TAIR
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000577681 – 388Protein phosphatase 2C 57Add BLAST388

Proteomic databases

PaxDbiP49599.

Expressioni

Gene expression databases

ExpressionAtlasiP49599. baseline and differential.
GenevisibleiP49599. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G27800.1.

Structurei

Secondary structure

1388
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi59 – 65Combined sources7
Beta strandi69 – 71Combined sources3
Beta strandi74 – 81Combined sources8
Turni82 – 85Combined sources4
Beta strandi86 – 98Combined sources13
Helixi99 – 115Combined sources17
Helixi119 – 123Combined sources5
Helixi127 – 151Combined sources25
Beta strandi160 – 169Combined sources10
Beta strandi172 – 180Combined sources9
Beta strandi182 – 187Combined sources6
Beta strandi190 – 193Combined sources4
Beta strandi202 – 204Combined sources3
Helixi205 – 216Combined sources12
Turni227 – 229Combined sources3
Helixi239 – 241Combined sources3
Turni242 – 244Combined sources3
Helixi245 – 254Combined sources10
Helixi260 – 264Combined sources5
Beta strandi272 – 274Combined sources3
Beta strandi278 – 283Combined sources6
Beta strandi288 – 294Combined sources7
Helixi296 – 299Combined sources4
Helixi304 – 318Combined sources15
Helixi321 – 334Combined sources14
Beta strandi341 – 347Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YZGX-ray1.60A/B59-351[»]
4YZHX-ray2.00A59-351[»]
ProteinModelPortaliP49599.
SMRiP49599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 348PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST290

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000029436.
InParanoidiP49599.
OMAiLYKECAA.
OrthoDBiEOG09360DLF.
PhylomeDBiP49599.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P49599-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALLRPHLHR FHSNTLRHSA YPSADAGGGL VVYPTYGRHR CSAIAIDAPS
60 70 80 90 100
SLTGVTPIRW GYTSVQGFRD EMEDDIVIRS DAVDSFSYAA VFDGHAGSSS
110 120 130 140 150
VKFLREELYK ECVGALQAGS LLNGGDFAAI KEALIKAFES VDRNLLKWLE
160 170 180 190 200
ANGDEEDESG STATVMIIRN DVSFIAHIGD SCAVLSRSGQ IEELTDYHRP
210 220 230 240 250
YGSSRAAIQE VKRVKEAGGW IVNGRICGDI AVSRAFGDIR FKTKKNDMLK
260 270 280 290 300
KGVDEGRWSE KFVSRIEFKG DMVVATPDIF QVPLTSDVEF IILASDGLWD
310 320 330 340 350
YMKSSDVVSY VRDQLRKHGN VQLACESLAQ VALDRRSQDN ISIIIADLGR
360 370 380
TEWKNLPAQR QNVVVELVQA ATTIGLVTVG IWMSSHLS
Length:388
Mass (Da):42,719
Last modified:January 24, 2001 - v2
Checksum:i496ECCC786AEB802
GO
Isoform 2 (identifier: P49599-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     334-335: DR → VK
     336-388: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:335
Mass (Da):36,863
Checksum:i5DD6AB6CDFC20D92
GO
Isoform 3 (identifier: P49599-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     323-326: LACE → ATCL
     327-388: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:326
Mass (Da):35,925
Checksum:i3554FE275D389482
GO

Sequence cautioni

The sequence AAA92889 differs from that shown. Reason: Frameshift at position 360.Curated
The sequence CAB43968 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 4MALL → SNSS in AAA92889 (PubMed:7569999).Curated4
Sequence conflicti69 – 70RD → QY in AAA92889 (PubMed:7569999).Curated2
Sequence conflicti271D → N in AAA92889 (PubMed:7569999).Curated1
Sequence conflicti353W → C in AAA92889 (PubMed:7569999).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034835323 – 326LACE → ATCL in isoform 3. Curated4
Alternative sequenceiVSP_034836327 – 388Missing in isoform 3. CuratedAdd BLAST62
Alternative sequenceiVSP_034837334 – 335DR → VK in isoform 2. Curated2
Alternative sequenceiVSP_034838336 – 388Missing in isoform 2. CuratedAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL078579 Genomic DNA. Translation: CAB43968.1. Sequence problems.
AL161571 Genomic DNA. Translation: CAB81429.1.
CP002687 Genomic DNA. Translation: AEE85393.1.
CP002687 Genomic DNA. Translation: AEE85394.1.
CP002687 Genomic DNA. Translation: AEE85395.1.
AY080875 mRNA. Translation: AAL87346.1.
AY114060 mRNA. Translation: AAM45108.1.
U34803 mRNA. Translation: AAA92889.1. Frameshift.
PIRiC85323.
T09019.
RefSeqiNP_194509.1. NM_118918.5. [P49599-1]
NP_849459.1. NM_179128.2. [P49599-2]
NP_849460.1. NM_179129.1. [P49599-3]
UniGeneiAt.23824.

Genome annotation databases

EnsemblPlantsiAT4G27800.1; AT4G27800.1; AT4G27800. [P49599-1]
GeneIDi828893.
GrameneiAT4G27800.1; AT4G27800.1; AT4G27800.
KEGGiath:AT4G27800.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL078579 Genomic DNA. Translation: CAB43968.1. Sequence problems.
AL161571 Genomic DNA. Translation: CAB81429.1.
CP002687 Genomic DNA. Translation: AEE85393.1.
CP002687 Genomic DNA. Translation: AEE85394.1.
CP002687 Genomic DNA. Translation: AEE85395.1.
AY080875 mRNA. Translation: AAL87346.1.
AY114060 mRNA. Translation: AAM45108.1.
U34803 mRNA. Translation: AAA92889.1. Frameshift.
PIRiC85323.
T09019.
RefSeqiNP_194509.1. NM_118918.5. [P49599-1]
NP_849459.1. NM_179128.2. [P49599-2]
NP_849460.1. NM_179129.1. [P49599-3]
UniGeneiAt.23824.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YZGX-ray1.60A/B59-351[»]
4YZHX-ray2.00A59-351[»]
ProteinModelPortaliP49599.
SMRiP49599.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G27800.1.

Proteomic databases

PaxDbiP49599.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G27800.1; AT4G27800.1; AT4G27800. [P49599-1]
GeneIDi828893.
GrameneiAT4G27800.1; AT4G27800.1; AT4G27800.
KEGGiath:AT4G27800.

Organism-specific databases

TAIRiAT4G27800.

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000029436.
InParanoidiP49599.
OMAiLYKECAA.
OrthoDBiEOG09360DLF.
PhylomeDBiP49599.

Miscellaneous databases

PROiP49599.

Gene expression databases

ExpressionAtlasiP49599. baseline and differential.
GenevisibleiP49599. AT.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2C57_ARATH
AccessioniPrimary (citable) accession number: P49599
Secondary accession number(s): Q3E9V3
, Q3E9V4, Q9M0J6, Q9STP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 24, 2001
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.