Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P49599 (P2C57_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 2C 57

Short name=AtPP2C57
EC=3.1.3.16
Alternative name(s):
Protein phosphatase 2C PPH1
Short name=PP2C PPH1
Gene names
Name:PPH1
Ordered Locus Names:At4g27800
ORF Names:T27E11.40
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Sequence caution

The sequence AAA92889.1 differs from that shown. Reason: Frameshift at position 360.

The sequence CAB43968.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P49599-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P49599-2)

The sequence of this isoform differs from the canonical sequence as follows:
     334-335: DR → VK
     336-388: Missing.
Note: Derived from EST data. No experimental confirmation available.
Isoform 3 (identifier: P49599-3)

The sequence of this isoform differs from the canonical sequence as follows:
     323-326: LACE → ATCL
     327-388: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 388388Protein phosphatase 2C 57
PRO_0000057768

Regions

Transmembrane363 – 38321Helical; Potential
Domain58 – 341284PP2C-like

Sites

Metal binding931Manganese 1 By similarity
Metal binding931Manganese 2 By similarity
Metal binding941Manganese 1; via carbonyl oxygen By similarity
Metal binding2961Manganese 2 By similarity
Metal binding3391Manganese 2 By similarity

Natural variations

Alternative sequence323 – 3264LACE → ATCL in isoform 3.
VSP_034835
Alternative sequence327 – 38862Missing in isoform 3.
VSP_034836
Alternative sequence334 – 3352DR → VK in isoform 2.
VSP_034837
Alternative sequence336 – 38853Missing in isoform 2.
VSP_034838

Experimental info

Sequence conflict1 – 44MALL → SNSS in AAA92889. Ref.4
Sequence conflict69 – 702RD → QY in AAA92889. Ref.4
Sequence conflict2711D → N in AAA92889. Ref.4
Sequence conflict3531W → C in AAA92889. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 24, 2001. Version 2.
Checksum: 496ECCC786AEB802

FASTA38842,719
        10         20         30         40         50         60 
MALLRPHLHR FHSNTLRHSA YPSADAGGGL VVYPTYGRHR CSAIAIDAPS SLTGVTPIRW 

        70         80         90        100        110        120 
GYTSVQGFRD EMEDDIVIRS DAVDSFSYAA VFDGHAGSSS VKFLREELYK ECVGALQAGS 

       130        140        150        160        170        180 
LLNGGDFAAI KEALIKAFES VDRNLLKWLE ANGDEEDESG STATVMIIRN DVSFIAHIGD 

       190        200        210        220        230        240 
SCAVLSRSGQ IEELTDYHRP YGSSRAAIQE VKRVKEAGGW IVNGRICGDI AVSRAFGDIR 

       250        260        270        280        290        300 
FKTKKNDMLK KGVDEGRWSE KFVSRIEFKG DMVVATPDIF QVPLTSDVEF IILASDGLWD 

       310        320        330        340        350        360 
YMKSSDVVSY VRDQLRKHGN VQLACESLAQ VALDRRSQDN ISIIIADLGR TEWKNLPAQR 

       370        380 
QNVVVELVQA ATTIGLVTVG IWMSSHLS 

« Hide

Isoform 2 [UniParc].

Checksum: 5DD6AB6CDFC20D92
Show »

FASTA33536,863
Isoform 3 [UniParc].

Checksum: 3554FE275D389482
Show »

FASTA32635,925

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Quantitative monitoring of gene expression patterns with a complementary DNA microarray."
Schena M., Shalon D., Davis R.W., Brown P.O.
Science 270:467-470(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-365 (ISOFORM 1).
Strain: cv. Columbia.
[5]"Plant PP2C phosphatases: emerging functions in stress signaling."
Schweighofer A., Hirt H., Meskiene I.
Trends Plant Sci. 9:236-243(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL078579 Genomic DNA. Translation: CAB43968.1. Sequence problems.
AL161571 Genomic DNA. Translation: CAB81429.1.
CP002687 Genomic DNA. Translation: AEE85393.1.
CP002687 Genomic DNA. Translation: AEE85394.1.
CP002687 Genomic DNA. Translation: AEE85395.1.
AY080875 mRNA. Translation: AAL87346.1.
AY114060 mRNA. Translation: AAM45108.1.
U34803 mRNA. Translation: AAA92889.1. Frameshift.
PIRC85323.
T09019.
RefSeqNP_194509.1. NM_118918.4.
NP_849459.1. NM_179128.1.
NP_849460.1. NM_179129.1.
UniGeneAt.23824.

3D structure databases

ProteinModelPortalP49599.
SMRP49599. Positions 60-348.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP49599.
PRIDEP49599.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G27800.1; AT4G27800.1; AT4G27800. [P49599-1]
GeneID828893.
KEGGath:AT4G27800.

Organism-specific databases

TAIRAT4G27800.

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000029436.
InParanoidP49599.
OMAIVNGRIC.
PhylomeDBP49599.
ProtClustDBCLSN2685522.

Enzyme and pathway databases

BioCycARA:AT4G27800-MONOMER.
ARA:GQT-2819-MONOMER.
ARA:GQT-2820-MONOMER.

Gene expression databases

ArrayExpressP49599.
GenevestigatorP49599.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP2C57_ARATH
AccessionPrimary (citable) accession number: P49599
Secondary accession number(s): Q3E9V3 expand/collapse secondary AC list , Q3E9V4, Q9M0J6, Q9STP6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 24, 2001
Last modified: April 16, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names