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P49599

- P2C57_ARATH

UniProt

P49599 - P2C57_ARATH

Protein

Protein phosphatase 2C 57

Gene

PPH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (24 Jan 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi93 – 931Manganese 1By similarity
    Metal bindingi93 – 931Manganese 2By similarity
    Metal bindingi94 – 941Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi296 – 2961Manganese 2By similarity
    Metal bindingi339 – 3391Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphatase activity Source: TAIR
    3. protein serine/threonine phosphatase activity Source: InterPro

    GO - Biological processi

    1. dephosphorylation Source: TAIR
    2. photosynthetic electron transport chain Source: TAIR
    3. photosystem stoichiometry adjustment Source: TAIR
    4. protein dephosphorylation Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT4G27800-MONOMER.
    ARA:GQT-2819-MONOMER.
    ARA:GQT-2820-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 2C 57 (EC:3.1.3.16)
    Short name:
    AtPP2C57
    Alternative name(s):
    Protein phosphatase 2C PPH1
    Short name:
    PP2C PPH1
    Gene namesi
    Name:PPH1
    Ordered Locus Names:At4g27800
    ORF Names:T27E11.40
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G27800.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR
    3. cytoplasm Source: TAIR
    4. integral component of membrane Source: UniProtKB-KW
    5. nucleolus Source: TAIR
    6. nucleus Source: TAIR
    7. thylakoid Source: TAIR

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 388388Protein phosphatase 2C 57PRO_0000057768Add
    BLAST

    Proteomic databases

    PaxDbiP49599.
    PRIDEiP49599.

    Expressioni

    Gene expression databases

    ArrayExpressiP49599.
    GenevestigatoriP49599.

    Structurei

    3D structure databases

    ProteinModelPortaliP49599.
    SMRiP49599. Positions 60-348.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei363 – 38321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini58 – 341284PP2C-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000029436.
    InParanoidiP49599.
    OMAiIVNGRIC.
    PhylomeDBiP49599.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P49599-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MALLRPHLHR FHSNTLRHSA YPSADAGGGL VVYPTYGRHR CSAIAIDAPS    50
    SLTGVTPIRW GYTSVQGFRD EMEDDIVIRS DAVDSFSYAA VFDGHAGSSS 100
    VKFLREELYK ECVGALQAGS LLNGGDFAAI KEALIKAFES VDRNLLKWLE 150
    ANGDEEDESG STATVMIIRN DVSFIAHIGD SCAVLSRSGQ IEELTDYHRP 200
    YGSSRAAIQE VKRVKEAGGW IVNGRICGDI AVSRAFGDIR FKTKKNDMLK 250
    KGVDEGRWSE KFVSRIEFKG DMVVATPDIF QVPLTSDVEF IILASDGLWD 300
    YMKSSDVVSY VRDQLRKHGN VQLACESLAQ VALDRRSQDN ISIIIADLGR 350
    TEWKNLPAQR QNVVVELVQA ATTIGLVTVG IWMSSHLS 388
    Length:388
    Mass (Da):42,719
    Last modified:January 24, 2001 - v2
    Checksum:i496ECCC786AEB802
    GO
    Isoform 2 (identifier: P49599-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         334-335: DR → VK
         336-388: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:335
    Mass (Da):36,863
    Checksum:i5DD6AB6CDFC20D92
    GO
    Isoform 3 (identifier: P49599-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         323-326: LACE → ATCL
         327-388: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:326
    Mass (Da):35,925
    Checksum:i3554FE275D389482
    GO

    Sequence cautioni

    The sequence AAA92889.1 differs from that shown. Reason: Frameshift at position 360.
    The sequence CAB43968.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 44MALL → SNSS in AAA92889. (PubMed:7569999)Curated
    Sequence conflicti69 – 702RD → QY in AAA92889. (PubMed:7569999)Curated
    Sequence conflicti271 – 2711D → N in AAA92889. (PubMed:7569999)Curated
    Sequence conflicti353 – 3531W → C in AAA92889. (PubMed:7569999)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei323 – 3264LACE → ATCL in isoform 3. CuratedVSP_034835
    Alternative sequencei327 – 38862Missing in isoform 3. CuratedVSP_034836Add
    BLAST
    Alternative sequencei334 – 3352DR → VK in isoform 2. CuratedVSP_034837
    Alternative sequencei336 – 38853Missing in isoform 2. CuratedVSP_034838Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL078579 Genomic DNA. Translation: CAB43968.1. Sequence problems.
    AL161571 Genomic DNA. Translation: CAB81429.1.
    CP002687 Genomic DNA. Translation: AEE85393.1.
    CP002687 Genomic DNA. Translation: AEE85394.1.
    CP002687 Genomic DNA. Translation: AEE85395.1.
    AY080875 mRNA. Translation: AAL87346.1.
    AY114060 mRNA. Translation: AAM45108.1.
    U34803 mRNA. Translation: AAA92889.1. Frameshift.
    PIRiC85323.
    T09019.
    RefSeqiNP_194509.1. NM_118918.4. [P49599-1]
    NP_849459.1. NM_179128.1. [P49599-2]
    NP_849460.1. NM_179129.1. [P49599-3]
    UniGeneiAt.23824.

    Genome annotation databases

    EnsemblPlantsiAT4G27800.1; AT4G27800.1; AT4G27800. [P49599-1]
    GeneIDi828893.
    KEGGiath:AT4G27800.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL078579 Genomic DNA. Translation: CAB43968.1 . Sequence problems.
    AL161571 Genomic DNA. Translation: CAB81429.1 .
    CP002687 Genomic DNA. Translation: AEE85393.1 .
    CP002687 Genomic DNA. Translation: AEE85394.1 .
    CP002687 Genomic DNA. Translation: AEE85395.1 .
    AY080875 mRNA. Translation: AAL87346.1 .
    AY114060 mRNA. Translation: AAM45108.1 .
    U34803 mRNA. Translation: AAA92889.1 . Frameshift.
    PIRi C85323.
    T09019.
    RefSeqi NP_194509.1. NM_118918.4. [P49599-1 ]
    NP_849459.1. NM_179128.1. [P49599-2 ]
    NP_849460.1. NM_179129.1. [P49599-3 ]
    UniGenei At.23824.

    3D structure databases

    ProteinModelPortali P49599.
    SMRi P49599. Positions 60-348.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P49599.
    PRIDEi P49599.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G27800.1 ; AT4G27800.1 ; AT4G27800 . [P49599-1 ]
    GeneIDi 828893.
    KEGGi ath:AT4G27800.

    Organism-specific databases

    TAIRi AT4G27800.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000029436.
    InParanoidi P49599.
    OMAi IVNGRIC.
    PhylomeDBi P49599.

    Enzyme and pathway databases

    BioCyci ARA:AT4G27800-MONOMER.
    ARA:GQT-2819-MONOMER.
    ARA:GQT-2820-MONOMER.

    Gene expression databases

    ArrayExpressi P49599.
    Genevestigatori P49599.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Quantitative monitoring of gene expression patterns with a complementary DNA microarray."
      Schena M., Shalon D., Davis R.W., Brown P.O.
      Science 270:467-470(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-365 (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Plant PP2C phosphatases: emerging functions in stress signaling."
      Schweighofer A., Hirt H., Meskiene I.
      Trends Plant Sci. 9:236-243(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiP2C57_ARATH
    AccessioniPrimary (citable) accession number: P49599
    Secondary accession number(s): Q3E9V3
    , Q3E9V4, Q9M0J6, Q9STP6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 24, 2001
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3