P49599 (P2C57_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C 57
Short name=AtPP2C57
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 2C PPH1
Short name=PP2C PPH1

Gene names

Name:	PPH1
Ordered Locus Names:	At4g27800
ORF Names:	T27E11.40

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence AAA92889.1 differs from that shown. Reason: Frameshift at position 360. The sequence CAB43968.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	photosynthetic electron transport chain Inferred from mutant phenotype PubMed 20126264. Source: TAIR photosystem stoichiometry adjustment Inferred from mutant phenotype PubMed 20126264PubMed 20176943. Source: TAIR protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast stroma Inferred from direct assay PubMed 20176943. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleolus Inferred from direct assay PubMed 15610358. Source: TAIR thylakoid Inferred from direct assay PubMed 20126264. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatase activity Inferred from direct assay PubMed 20126264. Source: TAIR protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P49599-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P49599-2)

The sequence of this isoform differs from the canonical sequence as follows:
     334-335: DR → VK
     336-388: Missing.

Note: Derived from EST data. No experimental confirmation available.

Isoform 3 (identifier: P49599-3)

The sequence of this isoform differs from the canonical sequence as follows:
     323-326: LACE → ATCL
     327-388: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Protein phosphatase 2C 57		PRO_0000057768
Regions
	Transmembrane	363 – 383	21	Helical; Potential
	Domain	58 – 341	284	PP2C-like
Sites
	Metal binding	93	1	Manganese 1 By similarity
	Metal binding	93	1	Manganese 2 By similarity
	Metal binding	94	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	296	1	Manganese 2 By similarity
	Metal binding	339	1	Manganese 2 By similarity
Natural variations
	Alternative sequence	323 – 326	4	LACE → ATCL in isoform 3.		VSP_034835
	Alternative sequence	327 – 388	62	Missing in isoform 3.		VSP_034836
	Alternative sequence	334 – 335	2	DR → VK in isoform 2.		VSP_034837
	Alternative sequence	336 – 388	53	Missing in isoform 2.		VSP_034838
Experimental info
	Sequence conflict	1 – 4	4	MALL → SNSS in AAA92889. Ref.4
	Sequence conflict	69 – 70	2	RD → QY in AAA92889. Ref.4
	Sequence conflict	271	1	D → N in AAA92889. Ref.4
	Sequence conflict	353	1	W → C in AAA92889. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 24, 2001. Version 2. Checksum: 496ECCC786AEB802	FASTA	388	42,719
	10 20 30 40 50 60 MALLRPHLHR FHSNTLRHSA YPSADAGGGL VVYPTYGRHR CSAIAIDAPS SLTGVTPIRW 70 80 90 100 110 120 GYTSVQGFRD EMEDDIVIRS DAVDSFSYAA VFDGHAGSSS VKFLREELYK ECVGALQAGS 130 140 150 160 170 180 LLNGGDFAAI KEALIKAFES VDRNLLKWLE ANGDEEDESG STATVMIIRN DVSFIAHIGD 190 200 210 220 230 240 SCAVLSRSGQ IEELTDYHRP YGSSRAAIQE VKRVKEAGGW IVNGRICGDI AVSRAFGDIR 250 260 270 280 290 300 FKTKKNDMLK KGVDEGRWSE KFVSRIEFKG DMVVATPDIF QVPLTSDVEF IILASDGLWD 310 320 330 340 350 360 YMKSSDVVSY VRDQLRKHGN VQLACESLAQ VALDRRSQDN ISIIIADLGR TEWKNLPAQR 370 380 QNVVVELVQA ATTIGLVTVG IWMSSHLS
	Isoform 2 [UniParc]. Checksum: 5DD6AB6CDFC20D92	FASTA	335	36,863
	10 20 30 40 50 60 MALLRPHLHR FHSNTLRHSA YPSADAGGGL VVYPTYGRHR CSAIAIDAPS SLTGVTPIRW 70 80 90 100 110 120 GYTSVQGFRD EMEDDIVIRS DAVDSFSYAA VFDGHAGSSS VKFLREELYK ECVGALQAGS 130 140 150 160 170 180 LLNGGDFAAI KEALIKAFES VDRNLLKWLE ANGDEEDESG STATVMIIRN DVSFIAHIGD 190 200 210 220 230 240 SCAVLSRSGQ IEELTDYHRP YGSSRAAIQE VKRVKEAGGW IVNGRICGDI AVSRAFGDIR 250 260 270 280 290 300 FKTKKNDMLK KGVDEGRWSE KFVSRIEFKG DMVVATPDIF QVPLTSDVEF IILASDGLWD 310 320 330 YMKSSDVVSY VRDQLRKHGN VQLACESLAQ VALVK
	Isoform 3 [UniParc]. Checksum: 3554FE275D389482	FASTA	326	35,925
	10 20 30 40 50 60 MALLRPHLHR FHSNTLRHSA YPSADAGGGL VVYPTYGRHR CSAIAIDAPS SLTGVTPIRW 70 80 90 100 110 120 GYTSVQGFRD EMEDDIVIRS DAVDSFSYAA VFDGHAGSSS VKFLREELYK ECVGALQAGS 130 140 150 160 170 180 LLNGGDFAAI KEALIKAFES VDRNLLKWLE ANGDEEDESG STATVMIIRN DVSFIAHIGD 190 200 210 220 230 240 SCAVLSRSGQ IEELTDYHRP YGSSRAAIQE VKRVKEAGGW IVNGRICGDI AVSRAFGDIR 250 260 270 280 290 300 FKTKKNDMLK KGVDEGRWSE KFVSRIEFKG DMVVATPDIF QVPLTSDVEF IILASDGLWD 310 320 YMKSSDVVSY VRDQLRKHGN VQATCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Quantitative monitoring of gene expression patterns with a complementary DNA microarray." Schena M., Shalon D., Davis R.W., Brown P.O. Science 270:467-470(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-365 (ISOFORM 1). Strain: cv. Columbia.
[5]	"Plant PP2C phosphatases: emerging functions in stress signaling." Schweighofer A., Hirt H., Meskiene I. Trends Plant Sci. 9:236-243(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL078579 Genomic DNA. Translation: CAB43968.1. Sequence problems. AL161571 Genomic DNA. Translation: CAB81429.1. CP002687 Genomic DNA. Translation: AEE85393.1. CP002687 Genomic DNA. Translation: AEE85394.1. CP002687 Genomic DNA. Translation: AEE85395.1. AY080875 mRNA. Translation: AAL87346.1. AY114060 mRNA. Translation: AAM45108.1. U34803 mRNA. Translation: AAA92889.1. Frameshift.
IPI	IPI00540406. IPI00544302. IPI00545112.
PIR	C85323. T09019.
RefSeq	NP_194509.1. NM_118918.4. NP_849459.1. NM_179128.1. NP_849460.1. NM_179129.1.
UniGene	At.23824.
3D structure databases
ProteinModelPortal	P49599.
SMR	P49599. Positions 60-348.
ModBase	Search...
Proteomic databases
PaxDb	P49599.
PRIDE	P49599.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G27800.1; AT4G27800.1; AT4G27800.
GeneID	828893.
KEGG	ath:AT4G27800.
Organism-specific databases
TAIR	At4g27800.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000029436.
InParanoid	P49599.
OMA	IVNGRIC.
PhylomeDB	P49599.
ProtClustDB	CLSN2685522.
Gene expression databases
ArrayExpress	P49599.
Genevestigator	P49599.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C57_ARATH

Accession

Primary (citable) accession number: P49599
Secondary accession number(s): Q3E9V3 , Q3E9V4, Q9M0J6, Q9STP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 24, 2001
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program