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Protein

Protein phosphatase 2C 37

Gene

PP2CA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major negative regulator of abscisic acid (ABA) responses during seed germination and cold acclimation. Confers insensitivity to ABA. Modulates negatively the AKT2/3 activity, which mediates K+ transport and membrane polarization during stress situations, probably by dephosphorylation. Prevents stomata closure by inactivating the S-type anion efflux channel SLAC1 and its activator SRK2E.7 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+1 Publication, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.1 Publication

Enzyme regulationi

Repressed by PYR/PYL/RCAR ABA receptors in an ABA-dependent manner.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi142Manganese 1By similarity1
Metal bindingi142Manganese 2Combined sources1 Publication1
Metal bindingi143Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi146ZincCombined sources1 Publication1
Metal bindingi148Zinc; via pros nitrogenCombined sources1 Publication1
Metal bindingi208ZincCombined sources1 Publication1
Metal bindingi210ZincCombined sources1 Publication1
Sitei280LockBy similarity1
Metal bindingi327Manganese 1Combined sources1 Publication1
Metal bindingi327Manganese 2Combined sources1 Publication1
Metal bindingi331Manganese 1Combined sources1 Publication1
Metal bindingi380Manganese 2Combined sources1 Publication1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phosphoprotein phosphatase activity Source: TAIR
  • protein kinase binding Source: UniProtKB
  • protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • negative regulation of abscisic acid-activated signaling pathway Source: TAIR
  • negative regulation of anion channel activity Source: UniProtKB
  • regulation of stomatal movement Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to cold Source: TAIR
  • response to water deprivation Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processAbscisic acid signaling pathway
LigandMagnesium, Manganese, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 37 (EC:3.1.3.16)
Short name:
AtPP2C37
Alternative name(s):
Protein ABA-HYPERSENSITIVE GERMINATION 3
Protein phosphatase 2C A
Short name:
PP2CA
Gene namesi
Name:PP2CA
Synonyms:AHG3
Ordered Locus Names:At3g11410
ORF Names:F24K9.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G11410
TAIRilocus:2080787 AT3G11410

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi139G → D: Loss of phosphatase activity. Loss of kinase activity but intact binding and repression of SRK2E; when associated with D-145. 3 Publications1
Mutagenesisi145G → D: Insensitive to ABA and loss of phosphatase activity. Loss of kinase activity but intact binding and repression of SRK2E; when associated with D-139. 3 Publications1
Mutagenesisi287G → E in ahg3-1; hypersensitivity to ABA during seed germination, and loss of phosphatase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000577691 – 399Protein phosphatase 2C 37Add BLAST399

Post-translational modificationi

Ubiquitinated by RGLG1 and RGLG5 in response to abscisic acid (ABA). Ubiquitination of PP2CA leads to its degradation by the proteasome.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiP49598
PRIDEiP49598

PTM databases

iPTMnetiP49598

Expressioni

Tissue specificityi

Mostly expressed in seeds and leaves, and, to a lower extent, in roots, stems, and flowers, particularly in siliques. Essentially found in the phloem.4 Publications

Inductioni

Repressed by MYB44. Induced by cold stress, drought, high salt, and ABA.7 Publications

Gene expression databases

ExpressionAtlasiP49598 baseline and differential
GenevisibleiP49598 AT

Interactioni

Subunit structurei

Interacts with AKT2/AKT3. Interacts with ABA-bounded PYR1, PYL1, PYL2, PYL3, PYL4, PYL9 and PYL12, and with free PYL2, PYL3, PYL4 and PYL13. Binds to and inactivates SLAC1 and SRK2E. The inactivation of SRK2E does not require phosphatase activity. Interacts with CBL1, CBL2, CBL3, CBL5, and CBL7, but not CBL4, CBL6, and CBL9 (PubMed:11181729, PubMed:12034902, PubMed:19407142, PubMed:19955427, PubMed:21596690, PubMed:24165892). Interacts with RGLG1 and RGLG5 (PubMed:27577789).7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi5648, 50 interactors
DIPiDIP-40197N
IntActiP49598, 15 interactors
MINTiP49598
STRINGi3702.AT3G11410.1

Structurei

Secondary structure

1399
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi105 – 110Combined sources6
Beta strandi119 – 132Combined sources14
Beta strandi134 – 147Combined sources14
Helixi148 – 165Combined sources18
Helixi173 – 189Combined sources17
Turni209 – 212Combined sources4
Helixi216 – 219Combined sources4
Beta strandi224 – 229Combined sources6
Beta strandi231 – 241Combined sources11
Beta strandi243 – 248Combined sources6
Beta strandi251 – 256Combined sources6
Helixi264 – 272Combined sources9
Beta strandi277 – 285Combined sources9
Turni286 – 288Combined sources3
Beta strandi289 – 293Combined sources5
Helixi298 – 300Combined sources3
Turni301 – 303Combined sources3
Beta strandi309 – 314Combined sources6
Beta strandi319 – 325Combined sources7
Helixi327 – 330Combined sources4
Helixi335 – 345Combined sources11
Helixi361 – 375Combined sources15
Beta strandi382 – 388Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4N0GX-ray2.38A/B72-399[»]
ProteinModelPortaliP49598
SMRiP49598
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 389PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST286

Domaini

The 'lock' site stabilizes the complex made of PP2C, ABA and PYR/PYL/RCAR receptor by keeping receptor 'gate' and 'latch' loops in closed positions.By similarity

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiKOG0698 Eukaryota
COG0631 LUCA
HOGENOMiHOG000233896
InParanoidiP49598
KOiK14497
OMAiHLIVANC
OrthoDBiEOG09360IAR
PhylomeDBiP49598

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
SMARTiView protein in SMART
SM00331 PP2C_SIG, 1 hit
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P49598-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGICCGVVG ETEPAAPVDS TSRASLRRRL DLLPSIKIVA DSAVAPPLEN
60 70 80 90 100
CRKRQKRETV VLSTLPGNLD LDSNVRSENK KARSAVTNSN SVTEAESFFS
110 120 130 140 150
DVPKIGTTSV CGRRRDMEDA VSIHPSFLQR NSENHHFYGV FDGHGCSHVA
160 170 180 190 200
EKCRERLHDI VKKEVEVMAS DEWTETMVKS FQKMDKEVSQ RECNLVVNGA
210 220 230 240 250
TRSMKNSCRC ELQSPQCDAV GSTAVVSVVT PEKIIVSNCG DSRAVLCRNG
260 270 280 290 300
VAIPLSVDHK PDRPDELIRI QQAGGRVIYW DGARVLGVLA MSRAIGDNYL
310 320 330 340 350
KPYVIPDPEV TVTDRTDEDE CLILASDGLW DVVPNETACG VARMCLRGAG
360 370 380 390
AGDDSDAAHN ACSDAALLLT KLALARQSSD NVSVVVVDLR KRRNNQASS
Length:399
Mass (Da):43,350
Last modified:February 1, 1996 - v1
Checksum:i83B82E32FEC71D4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38109 mRNA Translation: BAA07287.1
AC008153 Genomic DNA Translation: AAG51448.1
CP002686 Genomic DNA Translation: AEE75044.1
AY074368 mRNA Translation: AAL67064.1
AY091391 mRNA Translation: AAM14330.1
PIRiS55457
RefSeqiNP_187748.1, NM_111974.4
UniGeneiAt.20739

Genome annotation databases

EnsemblPlantsiAT3G11410.1; AT3G11410.1; AT3G11410
GeneIDi820314
GrameneiAT3G11410.1; AT3G11410.1; AT3G11410
KEGGiath:AT3G11410

Similar proteinsi

Entry informationi

Entry nameiP2C37_ARATH
AccessioniPrimary (citable) accession number: P49598
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 25, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health