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P49594 (FEM2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ca(2+)/calmodulin-dependent protein kinase phosphatase

Short name=CaM-kinase phosphatase
Short name=CaMKPase
EC=3.1.3.16
Alternative name(s):
Feminization of XX and XO animals protein 2
Sex-determining protein fem-2
Gene names
Name:fem-2
ORF Names:T19C3.8
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable phosphatase involved in the regulation of sex determination. Plays an important role in regulating a pathway transducing a non-cell-autonomous signal to a nuclear transcription factor. Promotes apoptosis. Together with fem-3 associates with the CBC(fem-1) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of tra-1. Ref.4

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Interacts with fem-1 and fem-3. Part of a E3 ubiquitin-protein ligase complex including fem-1, fem-2, fem-3, tra-1, cul-2 and elc-1. Ref.4

Sequence similarities

Belongs to the PP2C family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449Ca(2+)/calmodulin-dependent protein kinase phosphatase
PRO_0000057761

Sites

Metal binding2021Manganese 1 By similarity
Metal binding2021Manganese 2 By similarity
Metal binding2031Manganese 1; via carbonyl oxygen By similarity
Metal binding3701Manganese 2 By similarity
Metal binding4151Manganese 2 By similarity

Secondary structure

.................................................................... 449
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P49594 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: E51705B3DA0FF49D

FASTA44950,898
        10         20         30         40         50         60 
MEKVNEERDA VFEDHIGDRR RSVRSLLEEA FADEMEKTSY DVEVADTPQP HIPIRFRHPP 

        70         80         90        100        110        120 
IAGPVHDVFG DAIHDIFQKM MKRGQAVDFC HWVSHLIATE IDEKFSEVAF RDVQYNPDIY 

       130        140        150        160        170        180 
VTDSTTEAKK LFNDKIWPAI DKILQQNAET CPILSEKWSG IHVSGDQLKG QRHKQEDRFL 

       190        200        210        220        230        240 
AYPNGQYMDR GEDPISVLAV FDGHGGHECS QYAAGHLWET WLEVRKSRDP SDSLEDQLRK 

       250        260        270        280        290        300 
SLELLDERMT VRSVKECWKG GSTAVCCAID MDQKLMALAW LGDSPGYVMS NIEFRQLTRG 

       310        320        330        340        350        360 
HSPSDEREAR RVEEAGGQLF VIGGELRVNG VLNLTRALGD VPGRPMISNE PETCQVPIES 

       370        380        390        400        410        420 
SDYLVLLACD GISDVFNERD LYQLVEAFAN DYPVEDYAEL SRFICTKAIE AGSADNVSVV 

       430        440 
IGFLRPPQDV WKLMKHESDD EDSDVTDEE 

« Hide

References

« Hide 'large scale' references
[1]"The C. elegans sex-determining gene fem-2 encodes a putative protein phosphatase."
Pilgrim D.B., McGregor A., Jaeckle P., Johnson T., Hansen D.
Mol. Biol. Cell 6:1159-1171(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[3]"The Caenorhabditis elegans sex-determining protein fem-2 and its human homologue, hFEM-2, are Ca2+/calmodulin-dependent protein kinase phosphatases that promote apoptosis."
Tan K.M.L., Chan S.-L., Tan K.O., Yu V.C.
J. Biol. Chem. 276:44193-44202(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[4]"A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination."
Starostina N.G., Lim J.M., Schvarzstein M., Wells L., Spence A.M., Kipreos E.T.
Dev. Cell 13:127-139(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH FEM-1 AND FEM-3, IDENTIFICATION IN A COMPLEX WITH FEM-1; FEM-3; TRA-1; CUL-2 AND ELC-1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U29515 Genomic DNA. Translation: AAC06328.1.
FO081735 Genomic DNA. Translation: CCD73740.1.
PIRT16891.
RefSeqNP_497224.1. NM_064823.6.
UniGeneCel.7385.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4JNDX-ray1.65A1-449[»]
ProteinModelPortalP49594.
SMRP49594. Positions 13-436.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid40488. 6 interactions.
IntActP49594. 14 interactions.
STRING6239.T19C3.8.

Proteomic databases

PaxDbP49594.
PRIDEP49594.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaT19C3.8; T19C3.8; T19C3.8.
GeneID175217.
KEGGcel:CELE_T19C3.8.
UCSCT19C3.8. c. elegans.

Organism-specific databases

CTD175217.
WormBaseT19C3.8; CE02878; WBGene00001412; fem-2.

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000112566.
InParanoidP49594.
KOK17501.
OMAGHGGHEC.
OrthoDBEOG7X3QRM.
PhylomeDBP49594.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio887240.
PROP49594.

Entry information

Entry nameFEM2_CAEEL
AccessionPrimary (citable) accession number: P49594
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase