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Protein

Ca(2+)/calmodulin-dependent protein kinase phosphatase

Gene

fem-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable phosphatase involved in the regulation of sex determination. Plays an important role in regulating a pathway transducing a non-cell-autonomous signal to a nuclear transcription factor. Promotes apoptosis. Together with fem-3 associates with the CBC(fem-1) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of tra-1.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi202Manganese 1By similarity1
Metal bindingi202Manganese 2By similarity1
Metal bindingi203Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi370Manganese 2By similarity1
Metal bindingi415Manganese 2By similarity1

GO - Molecular functioni

  • calmodulin-dependent protein phosphatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • phosphoprotein phosphatase activity Source: WormBase
  • protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • male sex determination Source: WormBase
  • masculinization of hermaphroditic germ-line Source: WormBase
  • nematode male tail tip morphogenesis Source: WormBase
  • peptidyl-threonine dephosphorylation Source: UniProtKB
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • protein dephosphorylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ca(2+)/calmodulin-dependent protein kinase phosphatase (EC:3.1.3.16)
Short name:
CaM-kinase phosphatase
Short name:
CaMKPase
Alternative name(s):
Feminization of XX and XO animals protein 2
Sex-determining protein fem-2
Gene namesi
Name:fem-2
ORF Names:T19C3.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiT19C3.8; CE02878; WBGene00001412; fem-2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000577611 – 449Ca(2+)/calmodulin-dependent protein kinase phosphataseAdd BLAST449

Proteomic databases

EPDiP49594.
PaxDbiP49594.
PeptideAtlasiP49594.
PRIDEiP49594.

Expressioni

Gene expression databases

BgeeiWBGene00001412.

Interactioni

Subunit structurei

Interacts with fem-1 and fem-3 (PubMed:17609115). Part of a E3 ubiquitin-protein ligase complex including fem-1, fem-2, fem-3, tra-1, cul-2 and elc-1 (PubMed:17609115). Interacts with sel-10 (PubMed:15306688).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
fem-1P172213EBI-1998402,EBI-1998155
sel-10Q937942EBI-1998402,EBI-323098
tra-1P34708-12EBI-1998402,EBI-367214

Protein-protein interaction databases

BioGridi40488. 6 interactors.
IntActiP49594. 14 interactors.
STRINGi6239.T19C3.8.

Structurei

Secondary structure

1449
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 20Combined sources4
Helixi23 – 30Combined sources8
Helixi32 – 39Combined sources8
Helixi65 – 67Combined sources3
Helixi68 – 82Combined sources15
Helixi87 – 105Combined sources19
Helixi110 – 112Combined sources3
Helixi125 – 134Combined sources10
Helixi136 – 144Combined sources9
Turni148 – 150Combined sources3
Helixi152 – 155Combined sources4
Beta strandi163 – 168Combined sources6
Beta strandi171 – 174Combined sources4
Beta strandi177 – 184Combined sources8
Turni185 – 188Combined sources4
Beta strandi189 – 191Combined sources3
Beta strandi196 – 207Combined sources12
Helixi208 – 227Combined sources20
Helixi234 – 256Combined sources23
Beta strandi264 – 270Combined sources7
Turni271 – 274Combined sources4
Beta strandi275 – 283Combined sources9
Beta strandi286 – 292Combined sources7
Beta strandi294 – 296Combined sources3
Helixi306 – 314Combined sources9
Beta strandi319 – 322Combined sources4
Beta strandi325 – 328Combined sources4
Turni329 – 331Combined sources3
Helixi341 – 343Combined sources3
Turni344 – 346Combined sources3
Beta strandi352 – 357Combined sources6
Beta strandi362 – 368Combined sources7
Helixi370 – 373Combined sources4
Helixi378 – 391Combined sources14
Helixi394 – 399Combined sources6
Helixi400 – 410Combined sources11
Beta strandi417 – 425Combined sources9
Helixi427 – 434Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JNDX-ray1.65A1-449[»]
ProteinModelPortaliP49594.
SMRiP49594.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini160 – 424PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST265

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00840000129732.
HOGENOMiHOG000112566.
InParanoidiP49594.
KOiK17501.
OMAiGGHECSQ.
OrthoDBiEOG091G0CN4.
PhylomeDBiP49594.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKVNEERDA VFEDHIGDRR RSVRSLLEEA FADEMEKTSY DVEVADTPQP
60 70 80 90 100
HIPIRFRHPP IAGPVHDVFG DAIHDIFQKM MKRGQAVDFC HWVSHLIATE
110 120 130 140 150
IDEKFSEVAF RDVQYNPDIY VTDSTTEAKK LFNDKIWPAI DKILQQNAET
160 170 180 190 200
CPILSEKWSG IHVSGDQLKG QRHKQEDRFL AYPNGQYMDR GEDPISVLAV
210 220 230 240 250
FDGHGGHECS QYAAGHLWET WLEVRKSRDP SDSLEDQLRK SLELLDERMT
260 270 280 290 300
VRSVKECWKG GSTAVCCAID MDQKLMALAW LGDSPGYVMS NIEFRQLTRG
310 320 330 340 350
HSPSDEREAR RVEEAGGQLF VIGGELRVNG VLNLTRALGD VPGRPMISNE
360 370 380 390 400
PETCQVPIES SDYLVLLACD GISDVFNERD LYQLVEAFAN DYPVEDYAEL
410 420 430 440
SRFICTKAIE AGSADNVSVV IGFLRPPQDV WKLMKHESDD EDSDVTDEE
Length:449
Mass (Da):50,898
Last modified:October 1, 1996 - v2
Checksum:iE51705B3DA0FF49D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29515 Genomic DNA. Translation: AAC06328.1.
FO081735 Genomic DNA. Translation: CCD73740.1.
PIRiT16891.
RefSeqiNP_497224.1. NM_064823.6.
UniGeneiCel.7385.

Genome annotation databases

EnsemblMetazoaiT19C3.8; T19C3.8; WBGene00001412.
GeneIDi175217.
KEGGicel:CELE_T19C3.8.
UCSCiT19C3.8. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29515 Genomic DNA. Translation: AAC06328.1.
FO081735 Genomic DNA. Translation: CCD73740.1.
PIRiT16891.
RefSeqiNP_497224.1. NM_064823.6.
UniGeneiCel.7385.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JNDX-ray1.65A1-449[»]
ProteinModelPortaliP49594.
SMRiP49594.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi40488. 6 interactors.
IntActiP49594. 14 interactors.
STRINGi6239.T19C3.8.

Proteomic databases

EPDiP49594.
PaxDbiP49594.
PeptideAtlasiP49594.
PRIDEiP49594.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT19C3.8; T19C3.8; WBGene00001412.
GeneIDi175217.
KEGGicel:CELE_T19C3.8.
UCSCiT19C3.8. c. elegans.

Organism-specific databases

CTDi175217.
WormBaseiT19C3.8; CE02878; WBGene00001412; fem-2.

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00840000129732.
HOGENOMiHOG000112566.
InParanoidiP49594.
KOiK17501.
OMAiGGHECSQ.
OrthoDBiEOG091G0CN4.
PhylomeDBiP49594.

Miscellaneous databases

PROiP49594.

Gene expression databases

BgeeiWBGene00001412.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFEM2_CAEEL
AccessioniPrimary (citable) accession number: P49594
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.