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P49594

- FEM2_CAEEL

UniProt

P49594 - FEM2_CAEEL

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Protein
Ca(2+)/calmodulin-dependent protein kinase phosphatase
Gene
fem-2, T19C3.8
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Probable phosphatase involved in the regulation of sex determination. Plays an important role in regulating a pathway transducing a non-cell-autonomous signal to a nuclear transcription factor. Promotes apoptosis. Together with fem-3 associates with the CBC(fem-1) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of tra-1.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi202 – 2021Manganese 1 By similarity
Metal bindingi202 – 2021Manganese 2 By similarity
Metal bindingi203 – 2031Manganese 1; via carbonyl oxygen By similarity
Metal bindingi370 – 3701Manganese 2 By similarity
Metal bindingi415 – 4151Manganese 2 By similarity

GO - Molecular functioni

  1. calmodulin-dependent protein phosphatase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoprotein phosphatase activity Source: WormBase
  4. protein binding Source: UniProtKB
  5. protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. male sex determination Source: WormBase
  2. masculinization of hermaphroditic germ-line Source: WormBase
  3. peptidyl-threonine dephosphorylation Source: UniProtKB
  4. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  5. protein dephosphorylation Source: WormBase
  6. tail tip morphogenesis Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ca(2+)/calmodulin-dependent protein kinase phosphatase (EC:3.1.3.16)
Short name:
CaM-kinase phosphatase
Short name:
CaMKPase
Alternative name(s):
Feminization of XX and XO animals protein 2
Sex-determining protein fem-2
Gene namesi
Name:fem-2
ORF Names:T19C3.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiT19C3.8; CE02878; WBGene00001412; fem-2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Ca(2+)/calmodulin-dependent protein kinase phosphatase
PRO_0000057761Add
BLAST

Proteomic databases

PaxDbiP49594.
PRIDEiP49594.

Interactioni

Subunit structurei

Interacts with fem-1 and fem-3. Part of a E3 ubiquitin-protein ligase complex including fem-1, fem-2, fem-3, tra-1, cul-2 and elc-1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
fem-1P172213EBI-1998402,EBI-1998155
sel-10Q937942EBI-1998402,EBI-323098
tra-1P34708-12EBI-1998402,EBI-367214

Protein-protein interaction databases

BioGridi40488. 6 interactions.
IntActiP49594. 14 interactions.
STRINGi6239.T19C3.8.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi17 – 204
Helixi23 – 308
Helixi32 – 398
Helixi65 – 673
Helixi68 – 8215
Helixi87 – 10519
Helixi110 – 1123
Helixi125 – 13410
Helixi136 – 1449
Turni148 – 1503
Helixi152 – 1554
Beta strandi163 – 1686
Beta strandi171 – 1744
Beta strandi177 – 1848
Turni185 – 1884
Beta strandi189 – 1913
Beta strandi196 – 20712
Helixi208 – 22720
Helixi234 – 25623
Beta strandi264 – 2707
Turni271 – 2744
Beta strandi275 – 2839
Beta strandi286 – 2927
Beta strandi294 – 2963
Helixi306 – 3149
Beta strandi319 – 3224
Beta strandi325 – 3284
Turni329 – 3313
Helixi341 – 3433
Turni344 – 3463
Beta strandi352 – 3576
Beta strandi362 – 3687
Helixi370 – 3734
Helixi378 – 39114
Helixi394 – 3996
Helixi400 – 41011
Beta strandi417 – 4259
Helixi427 – 4348

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4JNDX-ray1.65A1-449[»]
ProteinModelPortaliP49594.
SMRiP49594. Positions 13-436.

Family & Domainsi

Sequence similaritiesi

Belongs to the PP2C family.

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000114971.
HOGENOMiHOG000112566.
InParanoidiP49594.
KOiK17501.
OMAiGHGGHEC.
OrthoDBiEOG7X3QRM.
PhylomeDBiP49594.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49594-1 [UniParc]FASTAAdd to Basket

« Hide

MEKVNEERDA VFEDHIGDRR RSVRSLLEEA FADEMEKTSY DVEVADTPQP    50
HIPIRFRHPP IAGPVHDVFG DAIHDIFQKM MKRGQAVDFC HWVSHLIATE 100
IDEKFSEVAF RDVQYNPDIY VTDSTTEAKK LFNDKIWPAI DKILQQNAET 150
CPILSEKWSG IHVSGDQLKG QRHKQEDRFL AYPNGQYMDR GEDPISVLAV 200
FDGHGGHECS QYAAGHLWET WLEVRKSRDP SDSLEDQLRK SLELLDERMT 250
VRSVKECWKG GSTAVCCAID MDQKLMALAW LGDSPGYVMS NIEFRQLTRG 300
HSPSDEREAR RVEEAGGQLF VIGGELRVNG VLNLTRALGD VPGRPMISNE 350
PETCQVPIES SDYLVLLACD GISDVFNERD LYQLVEAFAN DYPVEDYAEL 400
SRFICTKAIE AGSADNVSVV IGFLRPPQDV WKLMKHESDD EDSDVTDEE 449
Length:449
Mass (Da):50,898
Last modified:October 1, 1996 - v2
Checksum:iE51705B3DA0FF49D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U29515 Genomic DNA. Translation: AAC06328.1.
FO081735 Genomic DNA. Translation: CCD73740.1.
PIRiT16891.
RefSeqiNP_497224.1. NM_064823.6.
UniGeneiCel.7385.

Genome annotation databases

EnsemblMetazoaiT19C3.8; T19C3.8; WBGene00001412.
GeneIDi175217.
KEGGicel:CELE_T19C3.8.
UCSCiT19C3.8. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U29515 Genomic DNA. Translation: AAC06328.1 .
FO081735 Genomic DNA. Translation: CCD73740.1 .
PIRi T16891.
RefSeqi NP_497224.1. NM_064823.6.
UniGenei Cel.7385.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4JND X-ray 1.65 A 1-449 [» ]
ProteinModelPortali P49594.
SMRi P49594. Positions 13-436.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 40488. 6 interactions.
IntActi P49594. 14 interactions.
STRINGi 6239.T19C3.8.

Proteomic databases

PaxDbi P49594.
PRIDEi P49594.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai T19C3.8 ; T19C3.8 ; WBGene00001412 .
GeneIDi 175217.
KEGGi cel:CELE_T19C3.8.
UCSCi T19C3.8. c. elegans.

Organism-specific databases

CTDi 175217.
WormBasei T19C3.8 ; CE02878 ; WBGene00001412 ; fem-2.

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00740000114971.
HOGENOMi HOG000112566.
InParanoidi P49594.
KOi K17501.
OMAi GHGGHEC.
OrthoDBi EOG7X3QRM.
PhylomeDBi P49594.

Miscellaneous databases

NextBioi 887240.
PROi P49594.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The C. elegans sex-determining gene fem-2 encodes a putative protein phosphatase."
    Pilgrim D.B., McGregor A., Jaeckle P., Johnson T., Hansen D.
    Mol. Biol. Cell 6:1159-1171(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "The Caenorhabditis elegans sex-determining protein fem-2 and its human homologue, hFEM-2, are Ca2+/calmodulin-dependent protein kinase phosphatases that promote apoptosis."
    Tan K.M.L., Chan S.-L., Tan K.O., Yu V.C.
    J. Biol. Chem. 276:44193-44202(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination."
    Starostina N.G., Lim J.M., Schvarzstein M., Wells L., Spence A.M., Kipreos E.T.
    Dev. Cell 13:127-139(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FEM-1 AND FEM-3, IDENTIFICATION IN A COMPLEX WITH FEM-1; FEM-3; TRA-1; CUL-2 AND ELC-1.

Entry informationi

Entry nameiFEM2_CAEEL
AccessioniPrimary (citable) accession number: P49594
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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