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P49594

- FEM2_CAEEL

UniProt

P49594 - FEM2_CAEEL

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Protein

Ca(2+)/calmodulin-dependent protein kinase phosphatase

Gene

fem-2

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probable phosphatase involved in the regulation of sex determination. Plays an important role in regulating a pathway transducing a non-cell-autonomous signal to a nuclear transcription factor. Promotes apoptosis. Together with fem-3 associates with the CBC(fem-1) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of tra-1.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi202 – 2021Manganese 1By similarity
Metal bindingi202 – 2021Manganese 2By similarity
Metal bindingi203 – 2031Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi370 – 3701Manganese 2By similarity
Metal bindingi415 – 4151Manganese 2By similarity

GO - Molecular functioni

  1. calmodulin-dependent protein phosphatase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoprotein phosphatase activity Source: WormBase
  4. protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. male sex determination Source: WormBase
  2. masculinization of hermaphroditic germ-line Source: WormBase
  3. nematode male tail tip morphogenesis Source: WormBase
  4. peptidyl-threonine dephosphorylation Source: UniProtKB
  5. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  6. protein dephosphorylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ca(2+)/calmodulin-dependent protein kinase phosphatase (EC:3.1.3.16)
Short name:
CaM-kinase phosphatase
Short name:
CaMKPase
Alternative name(s):
Feminization of XX and XO animals protein 2
Sex-determining protein fem-2
Gene namesi
Name:fem-2
ORF Names:T19C3.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiT19C3.8; CE02878; WBGene00001412; fem-2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Ca(2+)/calmodulin-dependent protein kinase phosphatasePRO_0000057761Add
BLAST

Proteomic databases

PaxDbiP49594.
PRIDEiP49594.

Interactioni

Subunit structurei

Interacts with fem-1 and fem-3. Part of a E3 ubiquitin-protein ligase complex including fem-1, fem-2, fem-3, tra-1, cul-2 and elc-1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
fem-1P172213EBI-1998402,EBI-1998155
sel-10Q937942EBI-1998402,EBI-323098
tra-1P34708-12EBI-1998402,EBI-367214

Protein-protein interaction databases

BioGridi40488. 6 interactions.
IntActiP49594. 14 interactions.
STRINGi6239.T19C3.8.

Structurei

Secondary structure

1
449
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi17 – 204Combined sources
Helixi23 – 308Combined sources
Helixi32 – 398Combined sources
Helixi65 – 673Combined sources
Helixi68 – 8215Combined sources
Helixi87 – 10519Combined sources
Helixi110 – 1123Combined sources
Helixi125 – 13410Combined sources
Helixi136 – 1449Combined sources
Turni148 – 1503Combined sources
Helixi152 – 1554Combined sources
Beta strandi163 – 1686Combined sources
Beta strandi171 – 1744Combined sources
Beta strandi177 – 1848Combined sources
Turni185 – 1884Combined sources
Beta strandi189 – 1913Combined sources
Beta strandi196 – 20712Combined sources
Helixi208 – 22720Combined sources
Helixi234 – 25623Combined sources
Beta strandi264 – 2707Combined sources
Turni271 – 2744Combined sources
Beta strandi275 – 2839Combined sources
Beta strandi286 – 2927Combined sources
Beta strandi294 – 2963Combined sources
Helixi306 – 3149Combined sources
Beta strandi319 – 3224Combined sources
Beta strandi325 – 3284Combined sources
Turni329 – 3313Combined sources
Helixi341 – 3433Combined sources
Turni344 – 3463Combined sources
Beta strandi352 – 3576Combined sources
Beta strandi362 – 3687Combined sources
Helixi370 – 3734Combined sources
Helixi378 – 39114Combined sources
Helixi394 – 3996Combined sources
Helixi400 – 41011Combined sources
Beta strandi417 – 4259Combined sources
Helixi427 – 4348Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4JNDX-ray1.65A1-449[»]
ProteinModelPortaliP49594.
SMRiP49594. Positions 13-436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000114971.
HOGENOMiHOG000112566.
InParanoidiP49594.
KOiK17501.
OMAiGHGGHEC.
OrthoDBiEOG7X3QRM.
PhylomeDBiP49594.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49594-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKVNEERDA VFEDHIGDRR RSVRSLLEEA FADEMEKTSY DVEVADTPQP
60 70 80 90 100
HIPIRFRHPP IAGPVHDVFG DAIHDIFQKM MKRGQAVDFC HWVSHLIATE
110 120 130 140 150
IDEKFSEVAF RDVQYNPDIY VTDSTTEAKK LFNDKIWPAI DKILQQNAET
160 170 180 190 200
CPILSEKWSG IHVSGDQLKG QRHKQEDRFL AYPNGQYMDR GEDPISVLAV
210 220 230 240 250
FDGHGGHECS QYAAGHLWET WLEVRKSRDP SDSLEDQLRK SLELLDERMT
260 270 280 290 300
VRSVKECWKG GSTAVCCAID MDQKLMALAW LGDSPGYVMS NIEFRQLTRG
310 320 330 340 350
HSPSDEREAR RVEEAGGQLF VIGGELRVNG VLNLTRALGD VPGRPMISNE
360 370 380 390 400
PETCQVPIES SDYLVLLACD GISDVFNERD LYQLVEAFAN DYPVEDYAEL
410 420 430 440
SRFICTKAIE AGSADNVSVV IGFLRPPQDV WKLMKHESDD EDSDVTDEE
Length:449
Mass (Da):50,898
Last modified:October 1, 1996 - v2
Checksum:iE51705B3DA0FF49D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29515 Genomic DNA. Translation: AAC06328.1.
FO081735 Genomic DNA. Translation: CCD73740.1.
PIRiT16891.
RefSeqiNP_497224.1. NM_064823.6.
UniGeneiCel.7385.

Genome annotation databases

EnsemblMetazoaiT19C3.8; T19C3.8; WBGene00001412.
GeneIDi175217.
KEGGicel:CELE_T19C3.8.
UCSCiT19C3.8. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29515 Genomic DNA. Translation: AAC06328.1 .
FO081735 Genomic DNA. Translation: CCD73740.1 .
PIRi T16891.
RefSeqi NP_497224.1. NM_064823.6.
UniGenei Cel.7385.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4JND X-ray 1.65 A 1-449 [» ]
ProteinModelPortali P49594.
SMRi P49594. Positions 13-436.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 40488. 6 interactions.
IntActi P49594. 14 interactions.
STRINGi 6239.T19C3.8.

Proteomic databases

PaxDbi P49594.
PRIDEi P49594.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai T19C3.8 ; T19C3.8 ; WBGene00001412 .
GeneIDi 175217.
KEGGi cel:CELE_T19C3.8.
UCSCi T19C3.8. c. elegans.

Organism-specific databases

CTDi 175217.
WormBasei T19C3.8 ; CE02878 ; WBGene00001412 ; fem-2.

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00740000114971.
HOGENOMi HOG000112566.
InParanoidi P49594.
KOi K17501.
OMAi GHGGHEC.
OrthoDBi EOG7X3QRM.
PhylomeDBi P49594.

Miscellaneous databases

NextBioi 887240.
PROi P49594.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The C. elegans sex-determining gene fem-2 encodes a putative protein phosphatase."
    Pilgrim D.B., McGregor A., Jaeckle P., Johnson T., Hansen D.
    Mol. Biol. Cell 6:1159-1171(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "The Caenorhabditis elegans sex-determining protein fem-2 and its human homologue, hFEM-2, are Ca2+/calmodulin-dependent protein kinase phosphatases that promote apoptosis."
    Tan K.M.L., Chan S.-L., Tan K.O., Yu V.C.
    J. Biol. Chem. 276:44193-44202(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination."
    Starostina N.G., Lim J.M., Schvarzstein M., Wells L., Spence A.M., Kipreos E.T.
    Dev. Cell 13:127-139(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FEM-1 AND FEM-3, IDENTIFICATION IN A COMPLEX WITH FEM-1; FEM-3; TRA-1; CUL-2 AND ELC-1.

Entry informationi

Entry nameiFEM2_CAEEL
AccessioniPrimary (citable) accession number: P49594
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3