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Protein

Protein phosphatase 1F

Gene

PPM1F

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi198Manganese 1By similarity1
Metal bindingi198Manganese 2By similarity1
Metal bindingi199Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi360Manganese 2By similarity1
Metal bindingi404Manganese 2By similarity1

GO - Molecular functioni

  • calmodulin-dependent protein phosphatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • phosphatase activity Source: UniProtKB
  • protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  • cellular response to drug Source: UniProtKB
  • histone dephosphorylation Source: Ensembl
  • intrinsic apoptotic signaling pathway Source: UniProtKB
  • negative regulation of peptidyl-serine phosphorylation Source: UniProtKB
  • negative regulation of protein kinase activity Source: UniProtKB
  • negative regulation of protein kinase activity by regulation of protein phosphorylation Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • peptidyl-threonine dephosphorylation Source: UniProtKB
  • positive regulation of cell-substrate adhesion Source: UniProtKB
  • positive regulation of chemotaxis Source: UniProtKB
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • positive regulation of epithelial cell migration Source: UniProtKB
  • positive regulation of focal adhesion assembly Source: UniProtKB
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of growth Source: UniProtKB
  • positive regulation of stress fiber assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:HS01959-MONOMER.
SIGNORiP49593.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1F (EC:3.1.3.16)
Alternative name(s):
Ca(2+)/calmodulin-dependent protein kinase phosphatase
Short name:
CaM-kinase phosphatase
Short name:
CaMKPase
Partner of PIX 2
Protein fem-2 homolog
Short name:
hFem-2
Gene namesi
Name:PPM1F
Synonyms:KIAA0015, POPX2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:19388. PPM1F.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • perinuclear region of cytoplasm Source: Ensembl
  • protein complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi9647.
OpenTargetsiENSG00000100034.
PharmGKBiPA134935566.

Polymorphism and mutation databases

BioMutaiPPM1F.
DMDMi24638458.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000577581 – 454Protein phosphatase 1FAdd BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei454PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP49593.
MaxQBiP49593.
PaxDbiP49593.
PeptideAtlasiP49593.
PRIDEiP49593.

2D gel databases

OGPiP49593.

PTM databases

DEPODiP49593.
iPTMnetiP49593.
PhosphoSitePlusiP49593.

Expressioni

Gene expression databases

BgeeiENSG00000100034.
CleanExiHS_PPM1F.
ExpressionAtlasiP49593. baseline and differential.
GenevisibleiP49593. HS.

Organism-specific databases

HPAiHPA030989.
HPA030990.

Interactioni

Subunit structurei

Associates with FEM1B.

Binary interactionsi

WithEntry#Exp.IntActNotes
Camkk2Q8C0783EBI-719945,EBI-937199From a different organism.
DIAPH1O606103EBI-719945,EBI-3959709
FEM1BQ9UK732EBI-719945,EBI-310482

Protein-protein interaction databases

BioGridi115005. 14 interactors.
IntActiP49593. 11 interactors.
MINTiMINT-1397077.
STRINGi9606.ENSP00000263212.

Structurei

3D structure databases

ProteinModelPortaliP49593.
SMRiP49593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 413PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST258

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi101 – 107Poly-Glu7

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00840000129732.
HOGENOMiHOG000232094.
HOVERGENiHBG053656.
InParanoidiP49593.
KOiK17502.
OMAiGDVFQKP.
OrthoDBiEOG091G08KG.
PhylomeDBiP49593.
TreeFamiTF317617.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P49593-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSGAPQKSS PMASGAEETP GFLDTLLQDF PALLNPEDPL PWKAPGTVLS
60 70 80 90 100
QEEVEGELAE LAMGFLGSRK APPPLAAALA HEAVSQLLQT DLSEFRKLPR
110 120 130 140 150
EEEEEEEDDD EEEKAPVTLL DAQSLAQSFF NRLWEVAGQW QKQVPLAARA
160 170 180 190 200
SQRQWLVSIH AIRNTRRKME DRHVSLPSFN QLFGLSDPVN RAYFAVFDGH
210 220 230 240 250
GGVDAARYAA VHVHTNAARQ PELPTDPEGA LREAFRRTDQ MFLRKAKRER
260 270 280 290 300
LQSGTTGVCA LIAGATLHVA WLGDSQVILV QQGQVVKLME PHRPERQDEK
310 320 330 340 350
ARIEALGGFV SHMDCWRVNG TLAVSRAIGD VFQKPYVSGE ADAASRALTG
360 370 380 390 400
SEDYLLLACD GFFDVVPHQE VVGLVQSHLT RQQGSGLRVA EELVAAARER
410 420 430 440 450
GSHDNITVMV VFLRDPQELL EGGNQGEGDP QAEGRRQDLP SSLPEPETQA

PPRS
Length:454
Mass (Da):49,831
Last modified:November 1, 2002 - v3
Checksum:i2B49262333D4C9CF
GO
Isoform 2 (identifier: P49593-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MSSGAPQKSSPMASG → MGLCPSTRETAPPAV
     16-119: Missing.

Note: No experimental confirmation available.
Show »
Length:350
Mass (Da):38,518
Checksum:i3762283304FF2BA8
GO

Sequence cautioni

The sequence BAA02803 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti202G → D in AAL15579 (PubMed:11559703).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050620132R → C.Corresponds to variant rs9610645dbSNPEnsembl.1
Natural variantiVAR_036520296R → Q in a colorectal cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_036521417Q → K in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_024580420L → R.1 PublicationCorresponds to variant rs2070507dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0564831 – 15MSSGA…PMASG → MGLCPSTRETAPPAV in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_05648416 – 119Missing in isoform 2. 1 PublicationAdd BLAST104

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305840 mRNA. Translation: AAL15579.1.
AF520615 mRNA. Translation: AAM76059.1.
D13640 mRNA. Translation: BAA02803.2. Different initiation.
AK291628 mRNA. Translation: BAF84317.1.
AK294557 mRNA. Translation: BAH11809.1.
AP000555 Genomic DNA. No translation available.
D86995 Genomic DNA. No translation available.
D87012 Genomic DNA. No translation available.
D87019 Genomic DNA. No translation available.
CCDSiCCDS13796.1. [P49593-1]
RefSeqiNP_055449.1. NM_014634.3. [P49593-1]
UniGeneiHs.112728.

Genome annotation databases

EnsembliENST00000263212; ENSP00000263212; ENSG00000100034. [P49593-1]
GeneIDi9647.
KEGGihsa:9647.
UCSCiuc002zvp.3. human. [P49593-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305840 mRNA. Translation: AAL15579.1.
AF520615 mRNA. Translation: AAM76059.1.
D13640 mRNA. Translation: BAA02803.2. Different initiation.
AK291628 mRNA. Translation: BAF84317.1.
AK294557 mRNA. Translation: BAH11809.1.
AP000555 Genomic DNA. No translation available.
D86995 Genomic DNA. No translation available.
D87012 Genomic DNA. No translation available.
D87019 Genomic DNA. No translation available.
CCDSiCCDS13796.1. [P49593-1]
RefSeqiNP_055449.1. NM_014634.3. [P49593-1]
UniGeneiHs.112728.

3D structure databases

ProteinModelPortaliP49593.
SMRiP49593.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115005. 14 interactors.
IntActiP49593. 11 interactors.
MINTiMINT-1397077.
STRINGi9606.ENSP00000263212.

PTM databases

DEPODiP49593.
iPTMnetiP49593.
PhosphoSitePlusiP49593.

Polymorphism and mutation databases

BioMutaiPPM1F.
DMDMi24638458.

2D gel databases

OGPiP49593.

Proteomic databases

EPDiP49593.
MaxQBiP49593.
PaxDbiP49593.
PeptideAtlasiP49593.
PRIDEiP49593.

Protocols and materials databases

DNASUi9647.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263212; ENSP00000263212; ENSG00000100034. [P49593-1]
GeneIDi9647.
KEGGihsa:9647.
UCSCiuc002zvp.3. human. [P49593-1]

Organism-specific databases

CTDi9647.
DisGeNETi9647.
GeneCardsiPPM1F.
H-InvDBHIX0027933.
HGNCiHGNC:19388. PPM1F.
HPAiHPA030989.
HPA030990.
neXtProtiNX_P49593.
OpenTargetsiENSG00000100034.
PharmGKBiPA134935566.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00840000129732.
HOGENOMiHOG000232094.
HOVERGENiHBG053656.
InParanoidiP49593.
KOiK17502.
OMAiGDVFQKP.
OrthoDBiEOG091G08KG.
PhylomeDBiP49593.
TreeFamiTF317617.

Enzyme and pathway databases

BioCyciZFISH:HS01959-MONOMER.
SIGNORiP49593.

Miscellaneous databases

ChiTaRSiPPM1F. human.
GeneWikiiPPM1F.
GenomeRNAii9647.
PROiP49593.

Gene expression databases

BgeeiENSG00000100034.
CleanExiHS_PPM1F.
ExpressionAtlasiP49593. baseline and differential.
GenevisibleiP49593. HS.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPM1F_HUMAN
AccessioniPrimary (citable) accession number: P49593
Secondary accession number(s): A8K6G3, B7Z2C3, Q96PM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 2002
Last modified: November 2, 2016
This is version 157 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.