P49590 (SYHM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable histidine--tRNA ligase, mitochondrial
EC=6.1.1.21

Alternative name(s):
Histidine--tRNA ligase-like
Histidyl-tRNA synthetase
Short name=HisRS

Gene names

Name:	HARS2
Synonyms:	HARSL, HARSR, HO3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).
Subcellular location	Mitochondrion matrix By similarity.
Tissue specificity	A high level expression is seen in the heart, kidney and skeletal muscle while a lower level expression is seen in the brain and liver.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histidyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro tRNA aminoacylation for protein translation Traceable author statement. Source: Reactome
Cellular_component	mitochondrial matrix Traceable author statement. Source: Reactome
Molecular_function	ATP binding Non-traceable author statement. Source: UniProtKB histidine-tRNA ligase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Mitochondrion Potential
	Chain	34 – 506	473	Probable histidine--tRNA ligase, mitochondrial		PRO_0000136336
Amino acid modifications
	Modified residue	67	1	Phosphoserine Ref.3
	Modified residue	444	1	N6-acetyllysine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P49590 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: E1CE879837AE26E7	FASTA	506	56,888
	10 20 30 40 50 60 MPLLGLLPRR AWASLLSQLL RPPCASCTGA VRCQSQVAEA VLTSQLKAHQ EKPNFIIKTP 70 80 90 100 110 120 KGTRDLSPQH MVVREKILDL VISCFKRHGA KGMDTPAFEL KETLTEKYGE DSGLMYDLKD 130 140 150 160 170 180 QGGELLSLRY DLTVPFARYL AMNKVKKMKR YHVGKVWRRE SPTIVQGRYR EFCQCDFDIA 190 200 210 220 230 240 GQFDPMIPDA ECLKIMCEIL SGLQLGDFLI KVNDRRIVDG MFAVCGVPES KFRAICSSID 250 260 270 280 290 300 KLDKMAWKDV RHEMVVKKGL APEVADRIGD YVQCHGGVSL VEQMFQDPRL SQNKQALEGL 310 320 330 340 350 360 GDLKLLFEYL TLFGIADKIS FDLSLARGLD YYTGVIYEAV LLQTPTQAGE EPLNVGSVAA 370 380 390 400 410 420 GGRYDGLVGM FDPKGHKVPC VGLSIGVERI FYIVEQRMKT KGEKVRTTET QVFVATPQKN 430 440 450 460 470 480 FLQERLKLIA ELWDSGIKAE MLYKNNPKLL TQLHYCESTG IPLVVIIGEQ ELKEGVIKIR 490 500 SVASREEVAI KRENFVAEIQ KRLSES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel gene oriented in a head-to-head configuration with the human histidyl-tRNA synthetase (HRS) gene encodes an mRNA that predicts a polypeptide homologous to HRS." O'Hanlon T.P., Raben N., Miller F.W. Biochem. Biophys. Res. Commun. 210:556-566(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Skin.
[3]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[4]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-444, MASS SPECTROMETRY.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18936 Genomic DNA. Translation: AAA73972.1. U18937 mRNA. Translation: AAA73974.1. BC007680 mRNA. Translation: AAH07680.1. BC014982 mRNA. Translation: AAH14982.1.
IPI	IPI00027445.
PIR	I38913. I38915.
RefSeq	NP_036340.1. NM_012208.2.
UniGene	Hs.432560.
3D structure databases
ProteinModelPortal	P49590.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-59626N.
IntAct	P49590. 2 interactions.
STRING	9606.ENSP00000230771.
PTM databases
PhosphoSite	P49590.
Polymorphism databases
DMDM	1351156.
Proteomic databases
PaxDb	P49590.
PeptideAtlas	P49590.
PRIDE	P49590.
Protocols and materials databases
DNASU	23438.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000230771; ENSP00000230771; ENSG00000112855.
GeneID	23438.
KEGG	hsa:23438.
UCSC	uc003lgx.3. human.
Organism-specific databases
CTD	23438.
GeneCards	GC05P140053.
HGNC	HGNC:4817. HARS2.
HPA	HPA035941.
MIM	600783. gene.
neXtProt	NX_P49590.
Orphanet	2855. Perrault syndrome.
PharmGKB	PA29192.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0124.
HOGENOM	HOG000018075.
HOVERGEN	HBG002731.
InParanoid	P49590.
KO	K01892.
OMA	CGGGNFK.
OrthoDB	EOG4D26PM.
Enzyme and pathway databases
BRENDA	6.1.1.21. 2681.
Reactome	REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	P49590.
Bgee	P49590.
CleanEx	HS_HARS2.
Genevestigator	P49590.
GermOnline	ENSG00000112855. Homo sapiens.
Family and domain databases
Gene3D	3.40.50.800. 1 hit.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR015807. His-tRNA-ligase. IPR004516. HisRS/HisZ. [Graphical view]
PANTHER	PTHR11476. PTHR11476. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001549. His-tRNA_synth. 1 hit.
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
TIGRFAMs	TIGR00442. hisS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	23438.
NextBio	45705.
SOURCE	Search...

Entry information

Entry name

SYHM_HUMAN

Accession

Primary (citable) accession number: P49590

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.