Reviewed,
UniProtKB/Swiss-Prot P49588 (SYAC_HUMAN)
Last modified
June 16, 2009.
Version 81.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Alanyl-tRNA synthetase, cytoplasmic EC=6.1.1.7 Alternative name(s): Alanine--tRNA ligase Short name=AlaRS Renal carcinoma antigen NY-REN-42 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 968 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). |
| Subunit structure | Monomer. |
| Subcellular location | Cytoplasm Potential. |
| Sequence similarities | Belongs to the class-II aminoacyl-tRNA synthetase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein biosynthesis |
| Cellular component | Cytoplasm |
| Coding sequence diversity | Polymorphism |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Aminoacyl-tRNA synthetase Ligase |
| PTM | Acetylation Phosphoprotein |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | alanyl-tRNA aminoacylation Traceable author statement. Source: ProtInc |
| Cellular component | cytoplasm Ref.1 Traceable author statement. Source: ProtInc soluble fraction Ref.1Traceable author statement. Source: ProtInc |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activityInferred from electronic annotation. Source: EC tRNA binding Ref.1Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 968 | 968 | Alanyl-tRNA synthetase, cytoplasmic | PRO_0000075281 | |||||
Regions | |||||||||
| Region | 1 – 497 | 497 | Catalytic By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1 | 1 | N-acetylmethionine Ref.6 | ||||||
| Modified residue | 555 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 578 | 1 | Phosphothreonine Ref.8 | ||||||
| Modified residue | 580 | 1 | Phosphotyrosine Ref.8 | ||||||
Natural variations | |||||||||
| Natural variant | 275 | 1 | G → D: dbSNP rs11537667. | VAR_028204 | |||||
Experimental info | |||||||||
| Sequence conflict | 82 | 1 | H → Q in BAA06808. Ref.1 | ||||||
| Sequence conflict | 867 | 1 | S → T in BAD96544. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Human alanyl-tRNA synthetase: conservation in evolution of catalytic core and microhelix recognition." Shiba K., Ripmaster T.L., Suzuki N., Nichols R., Plotz P., Noda T., Schimmel P. Biochemistry 34:10340-10349(1995) [PubMed: 7654687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver. |
| [3] | "The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. Pennacchio L.A.Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta. |
| [6] | Bienvenut W.V., Glen H., Brunton V.G., Frame M.C. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-11; 304-320 AND 684-695, ACETYLATION AT MET-1, MASS SPECTROMETRY. Tissue: Osteosarcoma. |
| [7] | "Antigens recognized by autologous antibody in patients with renal-cell carcinoma." Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H., Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T., Old L.J. Int. J. Cancer 83:456-464(1999) [PubMed: 10508479] [Abstract] Cited for: IDENTIFICATION AS A RENAL CANCER ANTIGEN. Tissue: Renal cell carcinoma. |
| [8] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-578 AND TYR-580, MASS SPECTROMETRY. Tissue: Epithelium. |
| [9] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-555, MASS SPECTROMETRY. |
| [10] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| D32050 mRNA. Translation: BAA06808.1. AK222824 mRNA. Translation: BAD96544.1. AC012184 Genomic DNA. No translation available. CH471241 Genomic DNA. Translation: EAW51839.1. BC011451 mRNA. Translation: AAH11451.1. | |
| IPI | IPI00027442. |
| PIR | I60107. |
| RefSeq | NP_001596.2. |
| UniGene | Hs.315137 |
3D structure databases | |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | P49588. |
Proteomic databases | |
| PRIDE | P49588. |
Genome annotation databases | |
| Ensembl | ENSG00000090861. Homo sapiens. [Contig view] |
| GeneID | 16. |
| KEGG | hsa:16. |
Organism-specific databases | |
| GeneCards | GC16M068843. |
| H-InvDB | HIX0021867. |
| HGNC | HGNC:20. AARS. |
| MIM | 601065. gene. |
| PharmGKB | PA24367. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOVERGEN | P49588. |
| OMA | P49588. ASGKHND. |
Enzyme and pathway databases | |
| BRENDA | 6.1.1.7. 247. |
Gene expression databases | |
| ArrayExpress | P49588. |
| Bgee | P49588. |
| CleanEx | HS_AARS. |
| GermOnline | ENSG00000090861. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view] |
| Pfam | PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view] |
| PRINTS | PR00980. TRNASYNTHALA. |
| TIGRFAMs | TIGR00344. alaS. 1 hit. |
| PROSITE | PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00160. L-Alanine. |
| NextBio | 39. |
| SOURCE | Search... |
Entry information
| Entry name | SYAC_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P49588 Secondary accession number(s): A6NF14, Q53GV7, Q96FA0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Aminoacyl-tRNA synthetases List of aminoacyl-tRNA synthetase entries |
| Human chromosome 16 Human chromosome 16: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


