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Protein

Choline-phosphate cytidylyltransferase A

Gene

Pcyt1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Controls phosphatidylcholine synthesis.

Catalytic activityi

CTP + phosphocholine = diphosphate + CDP-choline.By similarity

Enzyme regulationi

By phosphorylation.By similarity

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase A (Pcyt1a), Choline-phosphate cytidylyltransferase B (Pcyt1b)
  2. Cholinephosphotransferase 1 (Chpt1), Choline/ethanolaminephosphotransferase 1 (Cept1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei122 – 1221CTPBy similarity
Binding sitei122 – 1221SubstrateBy similarity
Binding sitei151 – 1511SubstrateBy similarity
Binding sitei173 – 1731CTPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 929CTPBy similarity
Nucleotide bindingi168 – 1692CTPBy similarity
Nucleotide bindingi196 – 2005CTPBy similarity

GO - Molecular functioni

GO - Biological processi

  • CDP-choline pathway Source: UniProtKB
  • phosphatidylcholine biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BRENDAi2.7.7.15. 3474.
ReactomeiR-MMU-1483191. Synthesis of PC.
UniPathwayiUPA00753; UER00739.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline-phosphate cytidylyltransferase A (EC:2.7.7.15By similarity)
Alternative name(s):
CCT-alpha
CTP:phosphocholine cytidylyltransferase A
Short name:
CCT A
Short name:
CT A
Phosphorylcholine transferase A
Gene namesi
Name:Pcyt1a
Synonyms:Ctpct, Pcyt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:88557. Pcyt1a.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • endoplasmic reticulum membrane Source: MGI
  • glycogen granule Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Choline-phosphate cytidylyltransferase APRO_0000208454Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei8 – 81N6-acetyllysineBy similarity
Modified residuei233 – 2331PhosphoserineBy similarity
Modified residuei315 – 3151PhosphoserineCombined sources
Modified residuei319 – 3191PhosphoserineCombined sources
Modified residuei321 – 3211PhosphoserineBy similarity
Modified residuei322 – 3221PhosphoserineBy similarity
Modified residuei323 – 3231PhosphoserineCombined sources
Modified residuei325 – 3251PhosphothreonineCombined sources
Modified residuei329 – 3291PhosphoserineBy similarity
Modified residuei331 – 3311PhosphoserineCombined sources
Modified residuei333 – 3331PhosphoserineBy similarity
Modified residuei342 – 3421PhosphothreonineCombined sources
Modified residuei343 – 3431PhosphoserineCombined sources
Modified residuei345 – 3451PhosphoserineBy similarity
Modified residuei346 – 3461PhosphoserineBy similarity
Modified residuei347 – 3471PhosphoserineCombined sources
Modified residuei350 – 3501PhosphoserineBy similarity
Modified residuei352 – 3521PhosphoserineBy similarity
Modified residuei358 – 3581PhosphothreonineCombined sources
Modified residuei362 – 3621PhosphoserineCombined sources

Post-translational modificationi

The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation (By similarity).By similarity
Monoubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP49586.
MaxQBiP49586.
PaxDbiP49586.
PeptideAtlasiP49586.
PRIDEiP49586.

PTM databases

iPTMnetiP49586.
PhosphoSiteiP49586.

Expressioni

Gene expression databases

BgeeiENSMUSG00000005615.
ExpressionAtlasiP49586. baseline and differential.
GenevisibleiP49586. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi198967. 3 interactions.
IntActiP49586. 2 interactions.
MINTiMINT-4106778.
STRINGi10090.ENSMUSP00000078721.

Structurei

3D structure databases

ProteinModelPortaliP49586.
SMRiP49586. Positions 40-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati319 – 32461
Repeati329 – 33352; approximate
Repeati343 – 34863

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni228 – 28760AmphipathicSequence analysisAdd
BLAST
Regioni256 – 288333 X 11 AA approximate tandem repeatsAdd
BLAST
Regioni319 – 348303 X repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the cytidylyltransferase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2804. Eukaryota.
COG0615. LUCA.
GeneTreeiENSGT00390000000269.
HOGENOMiHOG000230945.
HOVERGENiHBG053531.
InParanoidiP49586.
KOiK00968.
OMAiSKMPRCA.
OrthoDBiEOG091G0DDW.
PhylomeDBiP49586.
TreeFamiTF106336.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

P49586-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAQSSAKVN SRKRRKEAPG PNGATEEDGI PSKVQRCAVG LRQPAPFSDE
60 70 80 90 100
IEVDFSKPYV RVTMEEACRG TPCERPVRVY ADGIFDLFHS GHARALMQAK
110 120 130 140 150
NLFPNTYLIV GVCSDELTHN FKGFTVMNEN ERYDAVQHCR YVDEVVRNAP
160 170 180 190 200
WTLTPEFLAE HRIDFVAHDD IPYSSAGSDD VYKHIKDAGM FAPTQRTEGI
210 220 230 240 250
STSDIITRIV RDYDVYARRN LQRGYTAKEL NVSFINEKKY HLQERVDKVK
260 270 280 290 300
KKVKDVEEKS KEFVQKVEEK SIDLIQKWEE KSREFIGSFL EMFGPEGALK
310 320 330 340 350
HMLKEGKGRM LQAISPKQSP SSSPTHERSP SPSFRWPFSG KTSPSSSPAS
360
LSRCRAVTCD ISEDEED
Length:367
Mass (Da):41,667
Last modified:February 1, 1996 - v1
Checksum:i306B656D2EAAA2B3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti360 – 3601D → V in AAA53526 (PubMed:7918629).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12302 mRNA. Translation: CAA78172.1.
L28956 mRNA. Translation: AAA53526.1.
U84207
, U84200, U84201, U84202, U84203, U84204, U84205, U84206 Genomic DNA. Translation: AAB63446.1.
AK076050 mRNA. Translation: BAC36148.1.
AK076830 mRNA. Translation: BAC36497.1.
BC018313 mRNA. Translation: AAH18313.1.
CCDSiCCDS28120.1.
PIRiA49366. S24935.
RefSeqiNP_001156631.1. NM_001163159.1.
NP_001156632.1. NM_001163160.1.
NP_034111.1. NM_009981.4.
UniGeneiMm.98775.

Genome annotation databases

EnsembliENSMUST00000079791; ENSMUSP00000078721; ENSMUSG00000005615.
ENSMUST00000104893; ENSMUSP00000130056; ENSMUSG00000005615.
ENSMUST00000115140; ENSMUSP00000110793; ENSMUSG00000005615.
GeneIDi13026.
KEGGimmu:13026.
UCSCiuc007yyw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12302 mRNA. Translation: CAA78172.1.
L28956 mRNA. Translation: AAA53526.1.
U84207
, U84200, U84201, U84202, U84203, U84204, U84205, U84206 Genomic DNA. Translation: AAB63446.1.
AK076050 mRNA. Translation: BAC36148.1.
AK076830 mRNA. Translation: BAC36497.1.
BC018313 mRNA. Translation: AAH18313.1.
CCDSiCCDS28120.1.
PIRiA49366. S24935.
RefSeqiNP_001156631.1. NM_001163159.1.
NP_001156632.1. NM_001163160.1.
NP_034111.1. NM_009981.4.
UniGeneiMm.98775.

3D structure databases

ProteinModelPortaliP49586.
SMRiP49586. Positions 40-295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198967. 3 interactions.
IntActiP49586. 2 interactions.
MINTiMINT-4106778.
STRINGi10090.ENSMUSP00000078721.

PTM databases

iPTMnetiP49586.
PhosphoSiteiP49586.

Proteomic databases

EPDiP49586.
MaxQBiP49586.
PaxDbiP49586.
PeptideAtlasiP49586.
PRIDEiP49586.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079791; ENSMUSP00000078721; ENSMUSG00000005615.
ENSMUST00000104893; ENSMUSP00000130056; ENSMUSG00000005615.
ENSMUST00000115140; ENSMUSP00000110793; ENSMUSG00000005615.
GeneIDi13026.
KEGGimmu:13026.
UCSCiuc007yyw.2. mouse.

Organism-specific databases

CTDi5130.
MGIiMGI:88557. Pcyt1a.

Phylogenomic databases

eggNOGiKOG2804. Eukaryota.
COG0615. LUCA.
GeneTreeiENSGT00390000000269.
HOGENOMiHOG000230945.
HOVERGENiHBG053531.
InParanoidiP49586.
KOiK00968.
OMAiSKMPRCA.
OrthoDBiEOG091G0DDW.
PhylomeDBiP49586.
TreeFamiTF106336.

Enzyme and pathway databases

UniPathwayiUPA00753; UER00739.
BRENDAi2.7.7.15. 3474.
ReactomeiR-MMU-1483191. Synthesis of PC.

Miscellaneous databases

ChiTaRSiPcyt1a. mouse.
PROiP49586.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005615.
ExpressionAtlasiP49586. baseline and differential.
GenevisibleiP49586. MM.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPCY1A_MOUSE
AccessioniPrimary (citable) accession number: P49586
Secondary accession number(s): Q542W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.