P49582 (ACHA7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuronal acetylcholine receptor subunit alpha-7

Gene names

Name:	Chrna7
Synonyms:	Acra7

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Subunit structure	Homopentamer. Interacts with RIC3; which is required for proper folding and assembly By similarity.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ionic channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	B cell activation Inferred from mutant phenotype. Source: MGI T cell activation Inferred from mutant phenotype. Source: MGI associative learning Inferred from mutant phenotype. Source: MGI behavioral response to ethanol Inferred from mutant phenotype. Source: MGI behavioral response to nicotine Inferred from genetic interaction. Source: MGI endocytosis Inferred from mutant phenotype. Source: MGI generation of ovulation cycle rhythm Inferred from mutant phenotype. Source: MGI memory Inferred from mutant phenotype. Source: MGI negative regulation of inflammatory response Inferred from mutant phenotype. Source: MGI negative regulation of interleukin-1 beta production Inferred from mutant phenotype. Source: MGI negative regulation of interleukin-6 production Inferred from mutant phenotype. Source: MGI negative regulation of tumor necrosis factor production Inferred from mutant phenotype. Source: MGI positive regulation of heart rate involved in baroreceptor response to decreased systemic arterial blood pressure Inferred from mutant phenotype. Source: MGI regulation of norepinephrine secretion Inferred from mutant phenotype. Source: MGI regulation of synaptic transmission, dopaminergic Inferred from mutant phenotype. Source: MGI response to food Inferred from mutant phenotype. Source: MGI sperm motility Inferred from mutant phenotype. Source: MGI synaptic transmission, cholinergic Inferred from mutant phenotype. Source: MGI
Cellular component	apical plasma membrane Inferred from direct assay. Source: MGI cell junction Inferred from electronic annotation. Source: UniProtKB-KW external side of plasma membrane Inferred from direct assay. Source: MGI membrane fraction Inferred from direct assay. Source: MGI postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetylcholine-activated cation-selective channel activity Inferred from direct assay. Source: MGI receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 502	480	Neuronal acetylcholine receptor subunit alpha-7		PRO_0000000368
Regions
	Topological domain	23 – 230	208	Extracellular Potential
	Transmembrane	231 – 255	25	Helical; Potential
	Transmembrane	262 – 280	19	Helical; Potential
	Transmembrane	296 – 317	22	Helical; Potential
	Topological domain	318 – 469	152	Cytoplasmic Potential
	Transmembrane	470 – 490	21	Helical; Potential
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Disulfide bond	150 ↔ 164		By similarity
	Disulfide bond	212 ↔ 213		Associated with receptor activation By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49582 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: C9312E5226D120E3	FASTA	502	56,632
	10 20 30 40 50 60 MCGRRGGIWL ALAAALLHVS LQGEFQRRLY KELVKNYNPL ERPVANDSQP LTVYFSLSLL 70 80 90 100 110 120 QIMDVDEKNQ VLTTNIWLQM SWTDHYLQWN MSEYPGVKNV RFPDGQIWKP DILLYNSADE 130 140 150 160 170 180 RFDATFHTNV LVNASGHCQY LPPGIFKSSC YIDVRWFPFD VQQCKLKFGS WSYGGWSLDL 190 200 210 220 230 240 QMQEADISSY IPNGEWDLMG IPGKRNEKFY ECCKEPYPDV TYTVTMRRRT LYYGLNLLIP 250 260 270 280 290 300 CVLISALALL VFLLPADSGE KISLGITVLL SLTVFMLLVA EIMPATSDSV PLIAQYFAST 310 320 330 340 350 360 MIIVGLSVVV TVIVLRYHHH DPDGGKMPKW TRIILLNWCA WFLRMKRPGE DKVRPACQHK 370 380 390 400 410 420 PRRCSLASVE LSAGAGPPTS NGNLLYIGFR GLEGMHCAPT PDSGVVCGRL ACSPTHDEHL 430 440 450 460 470 480 MHGTHPSDGD PDLAKILEEV RYIANRFRCQ DESEVICSEW KFAACVVDRL CLMAFSVFTI 490 500 ICTIGILMSA PNFVEAVSKD FA

References

[1]	"Cloning and mapping of the mouse alpha 7-neuronal nicotinic acetylcholine receptor." Orr-Urtreger A., Seldin M.F., Baldini A., Beaudet A.L. Genomics 26:399-402(1995) [PubMed: 7601470] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L37663 mRNA. Translation: AAC42053.1.
IPI	IPI00115489.
PIR	A57175.
RefSeq	NP_031416.3. NM_007390.3.
UniGene	Mm.113464.
3D structure databases
ProteinModelPortal	P49582.
SMR	P49582. Positions 23-490.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48732N.
STRING	P49582.
PTM databases
PhosphoSite	P49582.
Proteomic databases
PRIDE	P49582.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032738; ENSMUSP00000032738; ENSMUSG00000030525.
GeneID	11441.
KEGG	mmu:11441.
Organism-specific databases
CTD	1139.
MGI	MGI:99779. Chrna7.
Phylogenomic databases
eggNOG	roNOG14608.
HOGENOM	HBG387619.
HOVERGEN	HBG003756.
InParanoid	P49582.
OMA	AMPGKKN.
OrthoDB	EOG483D4K.
PhylomeDB	P49582.
Gene expression databases
ArrayExpress	P49582.
Bgee	P49582.
Genevestigator	P49582.
GermOnline	ENSMUSG00000030525. Mus musculus.
Family and domain databases
InterPro	IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. IPR002394. Nicotinic_acetylcholine_rcpt. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
KO	K04809.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00254. NICOTINICR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	278744.
SOURCE	Search...

Entry information

Entry name

ACHA7_MOUSE

Accession

Primary (citable) accession number: P49582

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program