P49576 (PPX1_PARTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP-X homolog 1
EC=3.1.3.16

Gene names

Name:	Ppx1
ORF Names:	GSPATT00019262001

Organism

Paramecium tetraurelia [Reference proteome]

Taxonomic identifier

5888 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Alveolata › Ciliophora › Intramacronucleata › Oligohymenophorea › Peniculida › Parameciidae › Paramecium

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.
Sequence caution	The sequence CAK85545.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Serine/threonine-protein phosphatase PP-X homolog 1		PRO_0000058888
Sites
	Active site	112	1	Proton donor By similarity
	Metal binding	51	1	Iron By similarity
	Metal binding	53	1	Iron By similarity
	Metal binding	79	1	Iron By similarity
	Metal binding	79	1	Manganese By similarity
	Metal binding	111	1	Manganese By similarity
	Metal binding	161	1	Manganese By similarity
	Metal binding	235	1	Manganese By similarity
Experimental info
	Sequence conflict	16	1	N → D in AAA75081. Ref.1
	Sequence conflict	20	1	T → S in AAA75081. Ref.1
	Sequence conflict	47	1	T → A in AAA75081. Ref.1
	Sequence conflict	62	1	L → V in AAA75081. Ref.1
	Sequence conflict	130	1	K → E in AAA75081. Ref.1
	Sequence conflict	297	1	V → T in AAA75081. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49576 [UniParc]. Last modified October 23, 2007. Version 2. Checksum: 9A00E5F88F5C810D	FASTA	303	34,745
	10 20 30 40 50 60 MSDIDQWIET LKNGENLKET DVKILCNKAK DILNNEDNVI RVEAPVTICG DIHGQFYDLM 70 80 90 100 110 120 ELFKVGGDVP ETNYLFLGDF VDRGYNSVET FLLLLALKVR YPDQITLIRG NHESRQITQV 130 140 150 160 170 180 YGFYDECLRK YSTLNVWKYC TEVFDYLALA AVVNDNIFCV HGGLSPYIKT IDEIRIINRK 190 200 210 220 230 240 QEVPHEGVMC DLMWSDPDEI EGWSQSARGA GFVFGADVVK EFNRRNGISL ICRAHQLAME 250 260 270 280 290 300 GFKLMFDNSL VTVWSAPNYC YRCGNVASIL ELDENLKKYY KLFEAAPTDR AQNSKKVIAD YFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Russell C.B., Johnson J., Hinrichsen R.D. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Stock 51.
[2]	"Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia." Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M., Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A., Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R. , Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F., Le Mouel A., Lepere G., Malinsky S., Nowacki M., Nowak J.K., Plattner H., Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M., Weissenbach J., Scarpelli C., Schaechter V., Sperling L., Meyer E., Cohen J., Wincker P. Nature 444:171-178(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Stock d4-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U31445 Genomic DNA. Translation: AAA75081.1. CT868541 Genomic DNA. Translation: CAK85545.1. Sequence problems.
3D structure databases
ProteinModelPortal	P49576.
SMR	P49576. Positions 3-303.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
ProtClustDB	CLSZ2454640.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPX1_PARTE

Accession

Primary (citable) accession number: P49576
Secondary accession number(s): A0DR78

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)