P49572 (TRPC_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Indole-3-glycerol phosphate synthase, chloroplastic
Short name=IGPS
EC=4.1.1.48

Gene names

Ordered Locus Names:	At2g04400
ORF Names:	T1O3.19

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.
Subcellular location	Plastid › chloroplast Probable.
Sequence similarities	Belongs to the TrpC family.
Sequence caution	The sequence AAA60380.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Traceable author statement Ref.4. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR cytosol Inferred from direct assay. Source: TAIR
Molecular function	copper ion binding Inferred from direct assay. Source: TAIR indole-3-glycerol-phosphate synthase activity Inferred from genetic interaction Ref.4. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 65	65	Chloroplast Potential
	Chain	66 – 402	337	Indole-3-glycerol phosphate synthase, chloroplastic		PRO_0000035787
Experimental info
	Sequence conflict	177	1	T → P in AAA60380. Ref.4
	Sequence conflict	250	1	F → Y in AAM64536. Ref.3
	Sequence conflict	276	1	A → T in AAM64536. Ref.3
	Sequence conflict	337	1	L → LAL Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P49572 [UniParc]. Last modified January 10, 2003. Version 2. Checksum: 288B4925BAD9E0DC	FASTA	402	44,577
	10 20 30 40 50 60 MEGLVPVQRL PIKVASPSLY RCNNSVSIRR SISGFAMDRK INFRAPSQFS IRAQQSDLKE 70 80 90 100 110 120 SLAVSSSSVE DKGNVLRIKE WEVEMYQEEL AISQGIRIRR KPPSKAPLGY SGPFELRLHN 130 140 150 160 170 180 NDADSPRNIL EEITWYKDVE VSRMKELNPL DVLKKAVEDA PPTRDFVGAL RMAHKRTGFP 190 200 210 220 230 240 GLIAEVKKAS PSRGILKENF DPVEIAQAYE KGGAACLSVL TDQKYFQGGF ENLEAIRSAG 250 260 270 280 290 300 VKCPLLCKEF VVDPWQIYYA RTKGADAVLL IAAVLADLEI TFLLKICKKL SLAALVEVHD 310 320 330 340 350 360 EREMGRVLGI EGIELVGINN RSLETFEVDI SNTKKLLEGE HGRQIRERDM IVVGESGLFT 370 380 390 400 PDDIAYVQAA GVKAVLVGES IVKQNDPEKG IAGLFGRNIS HT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Isolation of cDNAs encoding the tryptophan pathway enzyme indole-3-glycerol phosphate synthase from Arabidopsis thaliana." Li J., Chen S., Zhu L., Last R.L. Plant Physiol. 108:877-878(1995) [PubMed: 7610197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-402. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006951 Genomic DNA. Translation: AAD25838.1. CP002685 Genomic DNA. Translation: AEC05831.1. AY086973 mRNA. Translation: AAM64536.1. U18770 mRNA. Translation: AAA60380.1. Different initiation.
IPI	IPI00531403.
PIR	B84457.
RefSeq	NP_178521.1. NM_126473.4.
UniGene	At.366. At.48418.
3D structure databases
ProteinModelPortal	P49572.
SMR	P49572. Positions 128-396.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49572. 1 interaction.
STRING	P49572.
Proteomic databases
PRIDE	P49572.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G04400.1; AT2G04400.1; AT2G04400.
GeneID	814980.
GenomeReviews	Gene locus AT2G04400 in contig CT485783_GR.
KEGG	ath:AT2G04400.
Organism-specific databases
TAIR	At2g04400.
Phylogenomic databases
eggNOG	KOG4201.
HOGENOM	HBG540956.
InParanoid	P49572.
OMA	LEYAVPH.
PhylomeDB	P49572.
ProtClustDB	PLN02460.
Gene expression databases
ArrayExpress	P49572.
Genevestigator	P49572.
GermOnline	AT2G04400. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR013798. Indole-3-glycerol_P_synth. IPR001468. Indole-3-GlycerolPSynthase_CS. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01609.
Pfam	PF00218. IGPS. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
PROSITE	PS00614. IGPS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPC_ARATH

Accession

Primary (citable) accession number: P49572
Secondary accession number(s): Q8LBV5, Q9SJC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 10, 2003
Last modified:	January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program