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Protein

Glutamate dehydrogenase 2, mitochondrial

Gene

GLUD2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission.

Catalytic activityi

L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84SubstrateBy similarity1
Active sitei183PROSITE-ProRule annotation1

GO - Molecular functioni

  • ADP binding Source: BHF-UCL
  • glutamate dehydrogenase (NAD+) activity Source: UniProtKB
  • glutamate dehydrogenase [NAD(P)+] activity Source: BHF-UCL
  • GTP binding Source: BHF-UCL
  • leucine binding Source: BHF-UCL

GO - Biological processi

  • glutamate biosynthetic process Source: BHF-UCL
  • glutamate catabolic process Source: BHF-UCL
  • glutamate metabolic process Source: UniProtKB
  • oxidation-reduction process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:HS00018-MONOMER.
BRENDAi1.4.1.3. 2681.
ReactomeiR-HSA-2151201. Transcriptional activation of mitochondrial biogenesis.
R-HSA-70614. Amino acid synthesis and interconversion (transamination).
SABIO-RKiP49448.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate dehydrogenase 2, mitochondrial (EC:1.4.1.3)
Short name:
GDH 2
Gene namesi
Name:GLUD2
Synonyms:GLUDP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000182890.4.
HGNCiHGNC:4336. GLUD2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi2747.
MalaCardsiGLUD2.
OpenTargetsiENSG00000182890.
PharmGKBiPA28738.

Chemistry databases

DrugBankiDB00142. L-Glutamic Acid.
DB00157. NADH.

Polymorphism and mutation databases

BioMutaiGLUD2.
DMDMi13432152.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 53Mitochondrion1 PublicationAdd BLAST53
ChainiPRO_000000720854 – 558Glutamate dehydrogenase 2, mitochondrialAdd BLAST505

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei68N6-succinyllysineBy similarity1
Modified residuei79PhosphoserineBy similarity1
Modified residuei84N6-acetyllysine; alternateBy similarity1
Modified residuei84N6-succinyllysine; alternateBy similarity1
Modified residuei110N6-acetyllysine; alternateBy similarity1
Modified residuei110N6-succinyllysine; alternateBy similarity1
Modified residuei128PhosphoserineBy similarity1
Modified residuei135PhosphotyrosineBy similarity1
Modified residuei162N6-acetyllysine; alternateBy similarity1
Modified residuei162N6-succinyllysine; alternateBy similarity1
Modified residuei171N6-acetyllysineBy similarity1
Modified residuei172ADP-ribosylcysteine1 Publication1
Modified residuei183N6-acetyllysine; alternateBy similarity1
Modified residuei183N6-succinyllysine; alternateBy similarity1
Modified residuei187N6-acetyllysineBy similarity1
Modified residuei191N6-acetyllysine; alternateBy similarity1
Modified residuei191N6-succinyllysine; alternateBy similarity1
Modified residuei200N6-succinyllysineBy similarity1
Modified residuei211N6-acetyllysineBy similarity1
Modified residuei326N6-acetyllysineBy similarity1
Modified residuei346N6-acetyllysine; alternateBy similarity1
Modified residuei346N6-succinyllysine; alternateBy similarity1
Modified residuei352N6-acetyllysine; alternateBy similarity1
Modified residuei352N6-succinyllysine; alternateBy similarity1
Modified residuei363N6-acetyllysine; alternateBy similarity1
Modified residuei363N6-succinyllysine; alternateBy similarity1
Modified residuei365N6-acetyllysine; alternateBy similarity1
Modified residuei365N6-succinyllysine; alternateBy similarity1
Modified residuei386N6-acetyllysineBy similarity1
Modified residuei390N6-acetyllysine; alternateBy similarity1
Modified residuei390N6-succinyllysine; alternateBy similarity1
Modified residuei399N6-acetyllysineBy similarity1
Modified residuei410PhosphothreonineBy similarity1
Modified residuei415N6-acetyllysine; alternateBy similarity1
Modified residuei415N6-succinyllysine; alternateBy similarity1
Modified residuei457N6-acetyllysine; alternateBy similarity1
Modified residuei457N6-succinyllysine; alternateBy similarity1
Modified residuei477N6-acetyllysine; alternateBy similarity1
Modified residuei477N6-succinyllysine; alternateBy similarity1
Modified residuei480N6-acetyllysine; alternateBy similarity1
Modified residuei480N6-succinyllysine; alternateBy similarity1
Modified residuei503N6-acetyllysine; alternateBy similarity1
Modified residuei503N6-succinyllysine; alternateBy similarity1
Modified residuei527N6-acetyllysine; alternateBy similarity1
Modified residuei527N6-succinyllysine; alternateBy similarity1
Modified residuei545N6-acetyllysine; alternateBy similarity1
Modified residuei545N6-succinyllysine; alternateBy similarity1
Modified residuei548N6-acetyllysineBy similarity1

Post-translational modificationi

Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer.

Keywords - PTMi

Acetylation, ADP-ribosylation, Phosphoprotein

Proteomic databases

EPDiP49448.
MaxQBiP49448.
PaxDbiP49448.
PeptideAtlasiP49448.
PRIDEiP49448.

2D gel databases

REPRODUCTION-2DPAGEiIPI00027146.

PTM databases

iPTMnetiP49448.
PhosphoSitePlusiP49448.
SwissPalmiP49448.

Expressioni

Tissue specificityi

Expressed in retina, testis and, at a lower level, brain.

Gene expression databases

BgeeiENSG00000182890.
ExpressionAtlasiP49448. baseline and differential.
GenevisibleiP49448. HS.

Organism-specific databases

HPAiHPA042492.
HPA044839.
HPA061369.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

BioGridi109009. 13 interactors.
IntActiP49448. 8 interactors.
MINTiMINT-1411373.
STRINGi9606.ENSP00000327589.

Structurei

3D structure databases

ProteinModelPortaliP49448.
SMRiP49448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2250. Eukaryota.
COG0334. LUCA.
GeneTreeiENSGT00390000000854.
HOGENOMiHOG000243801.
HOVERGENiHBG005479.
InParanoidiP49448.
KOiK00261.
OMAiVMVIPDM.
OrthoDBiEOG091G0KMT.
PhylomeDBiP49448.
TreeFamiTF313945.

Family and domain databases

CDDicd01076. NAD_bind_1_Glu_DH. 1 hit.
InterProiView protein in InterPro
IPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR036291. NAD(P)-bd_dom_sf.
IPR033922. NAD_bind_Glu_DH.
PfamiView protein in Pfam
PF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiView protein in SMART
SM00839. ELFV_dehydrog. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00074. GLFV_DEHYDROGENASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRYLAKALL PSRAGPAALG SAANHSAALL GRGRGQPAAA SQPGLALAAR
60 70 80 90 100
RHYSELVADR EDDPNFFKMV EGFFDRGASI VEDKLVKDLR TQESEEQKRN
110 120 130 140 150
RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI
160 170 180 190 200
RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTENELEK
210 220 230 240 250
ITRRFTMELA KKGFIGPGVD VPAPDMNTGE REMSWIADTY ASTIGHYDIN
260 270 280 290 300
AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFR
310 320 330 340 350
DKTFVVQGFG NVGLHSMRYL HRFGAKCIAV GESDGSIWNP DGIDPKELED
360 370 380 390 400
FKLQHGSILG FPKAKPYEGS ILEVDCDILI PAATEKQLTK SNAPRVKAKI
410 420 430 440 450
IAEGANGPTT PEADKIFLER NILVIPDLYL NAGGVTVSYF EWLKNLNHVS
460 470 480 490 500
YGRLTFKYER DSNYHLLLSV QESLERKFGK HGGTIPIVPT AEFQDSISGA
510 520 530 540 550
SEKDIVHSAL AYTMERSARQ IMHTAMKYNL GLDLRTAAYV NAIEKVFKVY

SEAGVTFT
Length:558
Mass (Da):61,434
Last modified:April 27, 2001 - v2
Checksum:iF222B1B2CB74A6EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti286E → Q in CAA46995 (PubMed:8207021).Curated1
Sequence conflicti286E → Q in AAA20969 (PubMed:8207021).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048867498S → A. Corresponds to variant dbSNP:rs9697983Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66310 Genomic DNA. Translation: CAA46995.1.
U08997 Genomic DNA. Translation: AAA20969.1.
AK313356 mRNA. Translation: BAG36157.1.
AC006144 Genomic DNA. Translation: AAD05030.1.
BC050732 mRNA. Translation: AAH50732.1.
CCDSiCCDS14603.1.
PIRiA53719.
RefSeqiNP_036216.2. NM_012084.3.
UniGeneiHs.368538.

Genome annotation databases

EnsembliENST00000328078; ENSP00000327589; ENSG00000182890.
GeneIDi2747.
KEGGihsa:2747.
UCSCiuc004eto.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDHE4_HUMAN
AccessioniPrimary (citable) accession number: P49448
Secondary accession number(s): B2R8G0, Q9UDQ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 27, 2001
Last modified: October 25, 2017
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families