Reviewed,
UniProtKB/Swiss-Prot P49448 (DHE4_HUMAN)
Last modified
January 19, 2010.
Version 87.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glutamate dehydrogenase 2, mitochondrial Short name=GDH 2 EC=1.4.1.3 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 558 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission. |
| Catalytic activity | L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H. |
| Subunit structure | Homohexamer By similarity. |
| Subcellular location | |
| Tissue specificity | Expressed in retina, testis and, at a lower level, brain. |
| Sequence similarities | Belongs to the Glu/Leu/Phe/Val dehydrogenases family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Mitochondrion |
| Coding sequence diversity | Polymorphism |
| Domain | Transit peptide |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| PTM | Acetylation |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | glutamate biosynthetic process Inferred from direct assay. Source: UniProtKB glutamate deamidationInferred from direct assay. Source: UniProtKB oxidation reduction Ref.1Inferred from direct assay. Source: UniProtKB |
| Cellular component | mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ADP binding Inferred from direct assay. Source: UniProtKB GTP bindingInferred from direct assay. Source: UniProtKB glutamate dehydrogenase [NAD(P)+] activityInferred from direct assay. Source: UniProtKB glutamate dehydrogenase activity Ref.1Inferred from direct assay. Source: UniProtKB leucine bindingInferred from direct assay. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 53 | 53 | Mitochondrion By similarity | ||||||
| Chain | 54 – 558 | 505 | Glutamate dehydrogenase 2, mitochondrial | PRO_0000007208 | |||||
Sites | |||||||||
| Active site | 183 | 1 | By similarity | ||||||
| Binding site | 84 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 415 | 1 | N6-acetyllysine Ref.6 | ||||||
| Modified residue | 457 | 1 | N6-acetyllysine Ref.6 | ||||||
| Modified residue | 527 | 1 | N6-acetyllysine Ref.6 | ||||||
| Modified residue | 545 | 1 | N6-acetyllysine Ref.6 | ||||||
Natural variations | |||||||||
| Natural variant | 498 | 1 | S → A: dbSNP rs9697983. | VAR_048867 | |||||
Experimental info | |||||||||
| Sequence conflict | 286 | 1 | E → Q in CAA46995. Ref.1 | ||||||
| Sequence conflict | 286 | 1 | E → Q in AAA20969. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Novel human glutamate dehydrogenase expressed in neural and testicular tissues and encoded by an X-linked intronless gene." Shashidharan P., Michaelidis T.M., Robakis N.K., Kresovali A., Papamatheakis J., Plaitakis A. J. Biol. Chem. 269:16971-16976(1994) [PubMed: 8207021] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Retina. |
| [2] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis. |
| [3] | "The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. Bentley D.R.Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin. |
| [5] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [6] | "Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-415; LYS-457; LYS-527 AND LYS-545, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X66310 Genomic DNA. Translation: CAA46995.1. U08997 Genomic DNA. Translation: AAA20969.1. AK313356 mRNA. Translation: BAG36157.1. AC006144 Genomic DNA. Translation: AAD05030.1. BC050732 mRNA. Translation: AAH50732.1. |
| IPI | IPI00027146. |
| PIR | A53719. |
| RefSeq | NP_036216.2. |
| UniGene | Hs.368538 |
3D structure databases | |
| SMR | P49448. Positions 63-558. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P49448. 2 interactions. |
| STRING | P49448. |
2-D gel databases | |
| REPRODUCTION-2DPAGE | IPI00027146. |
Proteomic databases | |
| PeptideAtlas | P49448. |
| PRIDE | P49448. |
Genome annotation databases | |
| Ensembl | ENST00000328078; ENSP00000327589; ENSG00000182890; Homo sapiens. [Genome view] |
| GeneID | 2747. |
| KEGG | hsa:2747. |
| NMPDR | fig|9606.3.peg.33334. |
| UCSC | uc004eto.1. human. |
Organism-specific databases | |
| CTD | 2747. |
| GeneCards | GC0XP120009. |
| H-InvDB | HIX0017030. |
| HGNC | HGNC:4336. GLUD2. |
| MIM | 300144. gene. |
| PharmGKB | PA28738. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG10069. |
| HOGENOM | HBG590661. |
| HOVERGEN | P49448. |
| InParanoid | P49448. |
| OMA | ENMIHAS. |
| OrthoDB | EOG98D3FP. |
| PhylomeDB | P49448. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MONOMER-11465. |
| BRENDA | 1.4.1.3. 247. |
Gene expression databases | |
| ArrayExpress | P49448. |
| Bgee | P49448. |
| Genevestigator | P49448. |
| GermOnline | ENSG00000182890. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR11606:SF2. GLFV_DH. 1 hit. |
| Pfam | PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view] |
| PRINTS | PR00082. GLFDHDRGNASE. |
| SMART | SM00839. ELFV_dehydrog. 1 hit. [Graphical view] |
| PROSITE | PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00142. L-Glutamic Acid. DB00157. NADH. |
| NextBio | 10828. |
| SOURCE | Search... |
Entry information
| Entry name | DHE4_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P49448 Secondary accession number(s): B2R8G0, Q9UDQ4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome X Human chromosome X: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


