P49447 (CY561_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b561

Alternative name(s):
Cytochrome b-561

Gene names

Name:

CYB561

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Secretory vesicle-specific electron transport protein.
Cofactor	Binds 2 heme groups non-covalently By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Contains 1 cytochrome b561 domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Non-traceable author statement Ref.1Ref.6. Source: UniProtKB
Cellular_component	integral to membrane Non-traceable author statement Ref.1Ref.6. Source: UniProtKB
Molecular_function	ferric-chelate reductase activity Inferred from sequence or structural similarity PubMed 14499595. Source: HGNC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW transmembrane electron transfer carrier Non-traceable author statement Ref.1Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Cytochrome b561		PRO_0000151027
Regions
	Topological domain	1 – 16	16	Cytoplasmic Potential
	Transmembrane	17 – 37	21	Helical; Potential
	Transmembrane	52 – 72	21	Helical; Potential
	Transmembrane	86 – 106	21	Helical; Potential
	Transmembrane	125 – 145	21	Helical; Potential
	Transmembrane	159 – 179	21	Helical; Potential
	Transmembrane	199 – 219	21	Helical; Potential
	Topological domain	220 – 251	32	Cytoplasmic Potential
	Domain	19 – 220	202	Cytochrome b561
Sites
	Metal binding	53	1	Iron (heme axial ligand) Potential
	Metal binding	87	1	Iron (heme axial ligand) Potential
	Metal binding	91	1	Iron (heme axial ligand) Potential
	Metal binding	109	1	Iron (heme axial ligand) Potential
	Metal binding	121	1	Iron (heme axial ligand) Potential
	Metal binding	160	1	Iron (heme axial ligand) Potential
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
Experimental info
	Sequence conflict	6	1	A → S in AAH02976. Ref.5
	Sequence conflict	171	1	S → P in AAC50212. Ref.1
	Sequence conflict	171	1	S → P in AAA50952. Ref.6
	Sequence conflict	243 – 244	2	TE → RQ in AAC50212. Ref.1
	Sequence conflict	243 – 244	2	TE → RQ in AAA50952. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P49447 [UniParc]. Last modified November 25, 2002. Version 2. Checksum: 8EE53AE4D2569B77	FASTA	251	27,559
	10 20 30 40 50 60 MEGGAAAATP TALPYYVAFS QLLGLTLVAM TGAWLGLYRG GIAWESDLQF NAHPLCMVIG 70 80 90 100 110 120 LIFLQGNALL VYRVFRNEAK RTTKVLHGLL HIFALVIALV GLVAVFDYHR KKGYADLYSL 130 140 150 160 170 180 HSWCGILVFV LYFVQWLVGF SFFLFPGASF SLRSRYRPQH IFFGATIFLL SVGTALLGLK 190 200 210 220 230 240 EALLFNLGGK YSAFEPEGVL ANVLGLLLAC FGGAVLYILT RADWKRPSQA EEQALSMDFK 250 TLTEGDSPGS Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic structure and expression of the human gene encoding cytochrome b561, an integral protein of the chromaffin granule membrane." Srivastava M. J. Biol. Chem. 270:22714-22720(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Peripheral blood.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Lung and PNS.
[6]	"Human cytochrome b561: a revised hypothesis for conformation in membranes which reconciles sequence and functional information." Srivastava M., Gibson K.R., Pollard H.B., Fleming P.J. Biochem. J. 303:915-921(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-251. Tissue: Caudate nucleus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U29462 , U29460, U29461, U29464, U29469 Genomic DNA. Translation: AAC50212.1. BT007096 mRNA. Translation: AAP35760.1. AK316606 mRNA. Translation: BAG38193.1. CH471109 Genomic DNA. Translation: EAW94322.1. CH471109 Genomic DNA. Translation: EAW94323.1. CH471109 Genomic DNA. Translation: EAW94326.1. BC000021 mRNA. Translation: AAH00021.1. BC002976 mRNA. Translation: AAH02976.1. BC091485 mRNA. Translation: AAH91485.1. U06715 mRNA. Translation: AAA50952.1.
IPI	IPI00027144.
PIR	S53321.
RefSeq	NP_001017916.1. NM_001017916.1. NP_001017917.1. NM_001017917.1. NP_001906.3. NM_001915.3.
UniGene	Hs.355264.
3D structure databases
ProteinModelPortal	P49447.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000354028.
Protein family/group databases
TCDB	5.B.6.1.1. cytochrome b561 (Cytb561) family.
PTM databases
PhosphoSite	P49447.
Polymorphism databases
DMDM	25453426.
Proteomic databases
PRIDE	P49447.
Protocols and materials databases
DNASU	1534.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000360793; ENSP00000354028; ENSG00000008283. ENST00000392975; ENSP00000376701; ENSG00000008283. ENST00000392976; ENSP00000376702; ENSG00000008283. ENST00000581573; ENSP00000462325; ENSG00000008283. ENST00000584291; ENSP00000462543; ENSG00000008283.
GeneID	1534.
KEGG	hsa:1534.
UCSC	uc002jap.3. human.
Organism-specific databases
CTD	1534.
GeneCards	GC17M061509.
HGNC	HGNC:2571. CYB561.
HPA	HPA014753.
MIM	600019. gene.
neXtProt	NX_P49447.
PharmGKB	PA27069.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG262597.
HOVERGEN	HBG054164.
InParanoid	P49447.
KO	K08360.
OrthoDB	EOG4K0QPN.
PhylomeDB	P49447.
Gene expression databases
ArrayExpress	P49447.
Bgee	P49447.
CleanEx	HS_CYB561.
Genevestigator	P49447.
GermOnline	ENSG00000008283. Homo sapiens.
Family and domain databases
InterPro	IPR006593. Cyt_b561/ferric_Rdtase_TM. IPR004877. Cyt_b561_euk. [Graphical view]
Pfam	PF03188. Cytochrom_B561. 1 hit. [Graphical view]
SMART	SM00665. B561. 1 hit. [Graphical view]
PROSITE	PS50939. CYTOCHROME_B561. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CYB561. human.
GenomeRNAi	1534.
NextBio	6341.
SOURCE	Search...

Entry information

Entry name

CY561_HUMAN

Accession

Primary (citable) accession number: P49447
Secondary accession number(s): B2RE96 , D3DU11, Q5BJG9, Q9BU05, Q9BWR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 25, 2002
Last modified:	April 3, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.