P49445 (PTN7_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 7
EC=3.1.3.48

Alternative name(s):
Hematopoietic protein-tyrosine phosphatase
Short name=HEPTP
Protein-tyrosine phosphatase LC-PTP

Gene names

Name:	Ptpn7
Synonyms:	Lcptp

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the regulation of T and B-lymphocyte development and signal transduction By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Enzyme regulation	Inhibited in cells after FCER1A triggering. Ref.2
Subcellular location	Cytoplasm By similarity. Cytoplasm › cytoskeleton Ref.2.
Post-translational modification	Oxidized at active site cysteine. Treatment with pervanadate (vanadate and H2O2) or with antigen enhanced oxidation of active site cysteine.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Molecular function	Hydrolase Protein phosphatase
PTM	Oxidation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from direct assay Ref.1. Source: RGD internal side of plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Tyrosine-protein phosphatase non-receptor type 7		PRO_0000094763
Regions
	Domain	97 – 349	253	Tyrosine-protein phosphatase
	Region	38 – 51	14	Interaction with MAP kinases By similarity
	Region	290 – 296	7	Substrate binding By similarity
Sites
	Active site	290	1	Phosphocysteine intermediate By similarity
	Binding site	257	1	Substrate By similarity
	Binding site	334	1	Substrate By similarity
Amino acid modifications
	Modified residue	44	1	Phosphoserine By similarity
	Modified residue	66	1	Phosphothreonine By similarity
	Modified residue	93	1	Phosphoserine By similarity
	Modified residue	143	1	Phosphoserine By similarity
	Modified residue	290	1	Cysteine sulfenic acid (-SOH)

Sequences

	Sequence		Length	Mass (Da)
	P49445 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 5B98E196DB633677	FASTA	359	40,314
	10 20 30 40 50 60 MVQACEGRSR AQLPTLSLGA DMTQPPPAKA PAKKHVRLQE RRGSSVALML DVRSLGTVEP 70 80 90 100 110 120 ICSVNTPREV TLHFLRTAGH PLTRWTLQHQ PPSPKQLEEE FLKIPSNFVN PEDLDIPGHA 130 140 150 160 170 180 SKDRYKTILP NPQSRVCLGR AHSQEDSDYI NANYIRGYDG KEKVYIATQG PMPNTVADFW 190 200 210 220 230 240 EMVWQEDVSL IVMLTQLREG KEKCVHYWPT EEEAYGPFQI RIQGMKEHPE YTVRHLTIQH 250 260 270 280 290 300 QQECRSVKHI LFSAWPDHQT PESAGPLLRL VAEVETPETA ANSGPIVVHC SAGIGRTGCF 310 320 330 340 350 IATRIGCQQL KARGEVDILG IVCQLRLDRG GMIQTAEQYQ FLHHTLALYA AQLPPETDP

References

[1]	"Aggregation of IgE receptors in rat basophilic leukemia 2H3 cells induces tyrosine phosphorylation of the cytosolic protein-tyrosine phosphatase HePTP." Swieter M., Berenstein E.H., Swaim W.D., Siraganian R.P. J. Biol. Chem. 270:21902-21906(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Down-regulation of protein-tyrosine phosphatases activates an immune receptor in the absence of its translocation into lipid rafts." Heneberg P., Draberova L., Bambouskova M., Pompach P., Draber P. J. Biol. Chem. 285:12787-12802(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, OXIDATION AT CYS-290, ENZYME REGULATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28356 mRNA. Translation: AAA84443.1.
IPI	IPI00194355.
RefSeq	NP_663716.1. NM_145683.1.
UniGene	Rn.10160.
3D structure databases
ProteinModelPortal	P49445.
SMR	P49445. Positions 65-354.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000007833.
PTM databases
PhosphoSite	P49445.
Proteomic databases
PaxDb	P49445.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000007833; ENSRNOP00000007833; ENSRNOG00000005807.
GeneID	246781.
KEGG	rno:246781.
UCSC	RGD:708516. rat.
Organism-specific databases
CTD	5778.
RGD	708516. Ptpn7.
Phylogenomic databases
eggNOG	COG5599.
GeneTree	ENSGT00700000104095.
HOGENOM	HOG000294188.
HOVERGEN	HBG001594.
InParanoid	P49445.
KO	K04458.
OMA	GQEKVYI.
OrthoDB	EOG4DJJWN.
Gene expression databases
Genevestigator	P49445.
GermOnline	ENSRNOG00000005807. Rattus norvegicus.
Family and domain databases
InterPro	IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR008356. Tyr_Pase_KIM-con. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PRINTS	PR01778. KIMPTPASE. PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	623877.

Entry information

Entry name

PTN7_RAT

Accession

Primary (citable) accession number: P49445

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program