Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein phosphatase 2C 1

Gene

GSPATT00029903001

Organism
Paramecium tetraurelia
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme with a broad specificity.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Manganese 1By similarity
Metal bindingi57 – 571Manganese 2By similarity
Metal bindingi58 – 581Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi237 – 2371Manganese 2By similarity
Metal bindingi289 – 2891Manganese 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 1 (EC:3.1.3.16)
Short name:
PP2C 1
Gene namesi
ORF Names:GSPATT00029903001
OrganismiParamecium tetraurelia
Taxonomic identifieri5888 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium
ProteomesiUP000000600 Componenti: Partially assembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Protein phosphatase 2C 1PRO_0000057765Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Interactioni

Protein-protein interaction databases

STRINGi412030.XP_001426670.1.

Structurei

3D structure databases

ProteinModelPortaliP49444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 298276PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP49444.
KOiK17499.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49444-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPYLSQPKR DKTTTTGQGK SVIFAASEMQ GWRNTMEDAH IHRPDIIQDV
60 70 80 90 100
SVFGVFDGHG GREVAQFVEK HFVDELLKNK NFKEQKFEEA LKETFLKMDE
110 120 130 140 150
LLLTPEGQKE LNQYKATDTD ESYAGCTANV ALIYKNTLYV ANAGDSRSVL
160 170 180 190 200
CRNNTNHDMS VDHKPDNPEE KSRIERAGGF VSDGRVNGNL NLSRALGDLE
210 220 230 240 250
YKRDNKLRSN EQLIIALPDV KKTELTPQDK FILMGCDGVF ETLNHQELLK
260 270 280 290 300
QVNSTIGQAQ VTEELLKKAA EDLLDQLLAP DTSQGTGCDN MTTILVYLRR
Length:300
Mass (Da):33,699
Last modified:October 23, 2007 - v2
Checksum:iB9EF98B4F78F24EC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441P → H in CAA85448 (PubMed:7806499).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36985 mRNA. Translation: CAA85448.1.
CT868001 Genomic DNA. Translation: CAK59272.1.
PIRiA55804.
RefSeqiXP_001426670.1. XM_001426633.1.
UniGeneiPte.1455.

Genome annotation databases

EnsemblProtistsiCAK59272; CAK59272; GSPATT00029903001.
GeneIDi5012454.
KEGGiptm:GSPATT00029903001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36985 mRNA. Translation: CAA85448.1.
CT868001 Genomic DNA. Translation: CAK59272.1.
PIRiA55804.
RefSeqiXP_001426670.1. XM_001426633.1.
UniGeneiPte.1455.

3D structure databases

ProteinModelPortaliP49444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi412030.XP_001426670.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiCAK59272; CAK59272; GSPATT00029903001.
GeneIDi5012454.
KEGGiptm:GSPATT00029903001.

Phylogenomic databases

InParanoidiP49444.
KOiK17499.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A membrane-bound protein phosphatase type 2C from Paramecium tetraurelia. Purification, characterization, and cloning."
    Klumpp S., Hanke C., Donella-Deana A., Beyer A., Kellner R., Pinna L.A., Schultz J.E.
    J. Biol. Chem. 269:32774-32780(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Stock 51.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Stock d4-2.

Entry informationi

Entry nameiPP2C1_PARTE
AccessioniPrimary (citable) accession number: P49444
Secondary accession number(s): A0BL55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 23, 2007
Last modified: June 24, 2015
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.