P49444 (PP2C1_PARTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C 1
Short name=PP2C 1
EC=3.1.3.16

Gene names

ORF Names:

GSPATT00029903001

Organism

Paramecium tetraurelia [Reference proteome]

Taxonomic identifier

5888 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Alveolata › Ciliophora › Intramacronucleata › Oligohymenophorea › Peniculida › Parameciidae › Paramecium

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Enzyme with a broad specificity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Membrane; Peripheral membrane protein.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Cellular component	Membrane
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Protein phosphatase 2C 1		PRO_0000057765
Sites
	Metal binding	57	1	Manganese 1 By similarity
	Metal binding	57	1	Manganese 2 By similarity
	Metal binding	58	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	237	1	Manganese 2 By similarity
	Metal binding	289	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	44	1	P → H in CAA85448. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49444 [UniParc]. Last modified October 23, 2007. Version 2. Checksum: B9EF98B4F78F24EC	FASTA	300	33,699
	10 20 30 40 50 60 MGPYLSQPKR DKTTTTGQGK SVIFAASEMQ GWRNTMEDAH IHRPDIIQDV SVFGVFDGHG 70 80 90 100 110 120 GREVAQFVEK HFVDELLKNK NFKEQKFEEA LKETFLKMDE LLLTPEGQKE LNQYKATDTD 130 140 150 160 170 180 ESYAGCTANV ALIYKNTLYV ANAGDSRSVL CRNNTNHDMS VDHKPDNPEE KSRIERAGGF 190 200 210 220 230 240 VSDGRVNGNL NLSRALGDLE YKRDNKLRSN EQLIIALPDV KKTELTPQDK FILMGCDGVF 250 260 270 280 290 300 ETLNHQELLK QVNSTIGQAQ VTEELLKKAA EDLLDQLLAP DTSQGTGCDN MTTILVYLRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A membrane-bound protein phosphatase type 2C from Paramecium tetraurelia. Purification, characterization, and cloning." Klumpp S., Hanke C., Donella-Deana A., Beyer A., Kellner R., Pinna L.A., Schultz J.E. J. Biol. Chem. 269:32774-32780(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Stock 51.
[2]	"Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia." Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M., Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A., Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R. , Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F., Le Mouel A., Lepere G., Malinsky S., Nowacki M., Nowak J.K., Plattner H., Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M., Weissenbach J., Scarpelli C., Schaechter V., Sperling L., Meyer E., Cohen J., Wincker P. Nature 444:171-178(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Stock d4-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36985 mRNA. Translation: CAA85448.1. CT868001 Genomic DNA. Translation: CAK59272.1.
PIR	A55804.
RefSeq	XP_001426670.1. XM_001426633.1.
UniGene	Pte.1455.
3D structure databases
ProteinModelPortal	P49444.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5012454.
KEGG	ptm:GSPATT00029903001.
Phylogenomic databases
ProtClustDB	CLSZ2448970.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP2C1_PARTE

Accession

Primary (citable) accession number: P49444
Secondary accession number(s): A0BL55

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	October 23, 2007
Last modified:	April 3, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)