P49443 (PPM1A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1A
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 2C isoform alpha
Short name=PP2C-alpha
Protein phosphatase IA

Gene names

Name:	Ppm1a
Synonyms:	Pppm1a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit.
Subunit structure	Monomer By similarity. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of SMAD protein complex assembly Inferred from electronic annotation. Source: Compara negative regulation of transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara peptidyl-threonine dephosphorylation Inferred from electronic annotation. Source: Compara positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from electronic annotation. Source: Compara positive regulation of Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara protein dephosphorylation Inferred from direct assay PubMed 14664809. Source: MGI
Cellular_component	neuron projection Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell voltage-gated calcium channel complex Inferred from electronic annotation. Source: Compara
Molecular_function	R-SMAD binding Inferred from sequence or structural similarity. Source: UniProtKB calmodulin-dependent protein phosphatase activity Inferred from electronic annotation. Source: Compara magnesium ion binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: InterPro phosphoprotein phosphatase activity Inferred from direct assay PubMed 14654243. Source: MGI signal transducer activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Protein phosphatase 1A		PRO_0000057742
Sites
	Metal binding	60	1	Manganese 1 By similarity
	Metal binding	60	1	Manganese 2 By similarity
	Metal binding	61	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	239	1	Manganese 2 By similarity
	Metal binding	282	1	Manganese 2 By similarity
Amino acid modifications
	Modified residue	375	1	Phosphoserine By similarity
	Modified residue	377	1	Phosphoserine Ref.3
	Lipidation	2	1	N-myristoyl glycine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49443 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: DC206610E1583870	FASTA	382	42,433
	10 20 30 40 50 60 MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL ETWSFFAVYD 70 80 90 100 110 120 GHAGSQVAKY CCEHLLDHIT NNQDFRGSAG APSVENVKNG IRTGFLEIDE HMRVMSEKKH 130 140 150 160 170 180 GADRSGSTAV GVLISPQHTY FINCGDSRGL LCRNRKVHFF TQDHKPSNPL EKERIQNAGG 190 200 210 220 230 240 SVMIQRVNGS LAVSRALGDF DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG 250 260 270 280 290 300 IWDVMGNEEL CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPSAPKVSAE 310 320 330 340 350 360 AVKKEAELDK YLESRVEEII KKQVEGVPDL VHVMRTLASE NIPSLPPGGE LASKRNVIEA 370 380 VYNRLNPYKN DDTDSASTDD MW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cDNA sequence encoding mouse Mg2+ -dependent protein phosphatase alpha." Kato S., Kobayashi T., Terasawa T., Ohnishi M., Sasahara Y., Kanamaru R., Tamura S. Gene 145:311-312(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D28117 mRNA. Translation: BAA05662.1. BC008595 mRNA. Translation: AAH08595.1.
IPI	IPI00114802.
PIR	I53823.
RefSeq	NP_032936.1. NM_008910.3.
UniGene	Mm.261045.
3D structure databases
ProteinModelPortal	P49443.
SMR	P49443. Positions 2-368.
ModBase	Search...
PTM databases
PhosphoSite	P49443.
Proteomic databases
PaxDb	P49443.
PRIDE	P49443.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000021514; ENSMUSP00000021514; ENSMUSG00000021096.
GeneID	19042.
KEGG	mmu:19042.
UCSC	uc007nvu.2. mouse.
Organism-specific databases
CTD	5494.
MGI	MGI:99878. Ppm1a.
Phylogenomic databases
eggNOG	COG0631.
GeneTree	ENSGT00650000093052.
HOGENOM	HOG000233895.
HOVERGEN	HBG053647.
InParanoid	P49443.
KO	K04457.
OMA	EVYAIER.
OrthoDB	EOG4GMTX1.
Gene expression databases
Bgee	P49443.
Genevestigator	P49443.
GermOnline	ENSMUSG00000021096. Mus musculus.
Family and domain databases
Gene3D	1.10.10.430. 1 hit. 3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR012911. PP2C_C. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. PF07830. PP2C_C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit. SSF81601. PP2C_C. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PPM1A. mouse.
NextBio	295493.
SOURCE	Search...

Entry information

Entry name

PPM1A_MOUSE

Accession

Primary (citable) accession number: P49443

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program