Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein phosphatase 1A

Gene

Ppm1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling (By similarity). Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+, Mn2+Note: Binds 2 magnesium or manganese ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60Manganese 1By similarity1
Metal bindingi60Manganese 2By similarity1
Metal bindingi61Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi239Manganese 2By similarity1
Metal bindingi282Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2173795 Downregulation of SMAD2/3:SMAD4 transcriptional activity
R-MMU-380972 Energy dependent regulation of mTOR by LKB1-AMPK

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1A (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2C isoform alpha
Short name:
PP2C-alpha
Protein phosphatase IA
Gene namesi
Name:Ppm1a
Synonyms:Pppm1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:99878 Ppm1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000577422 – 382Protein phosphatase 1AAdd BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei375PhosphoserineCombined sources1
Modified residuei377PhosphoserineCombined sources1

Post-translational modificationi

N-myristoylation is essential for the recognition of its substrates for dephosphorylation.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiP49443
MaxQBiP49443
PaxDbiP49443
PeptideAtlasiP49443
PRIDEiP49443

PTM databases

iPTMnetiP49443
PhosphoSitePlusiP49443
SwissPalmiP49443

Expressioni

Gene expression databases

BgeeiENSMUSG00000021096
ExpressionAtlasiP49443 baseline and differential
GenevisibleiP49443 MM

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling (By similarity). Interacts with the phosphorylated form of IKBKB/IKKB (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202332, 4 interactors
IntActiP49443, 2 interactors
MINTiP49443
STRINGi10090.ENSMUSP00000021514

Structurei

3D structure databases

ProteinModelPortaliP49443
SMRiP49443
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 291PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiKOG0697 Eukaryota
COG0631 LUCA
GeneTreeiENSGT00880000137909
HOGENOMiHOG000233895
HOVERGENiHBG053647
InParanoidiP49443
KOiK04457
OMAiVMSGRVN
OrthoDBiEOG091G0AJQ
PhylomeDBiP49443
TreeFamiTF313590

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di1.10.10.430, 1 hit
3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR012911 PP2C_C
IPR036580 PP2C_C_sf
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
PF07830 PP2C_C, 1 hit
SMARTiView protein in SMART
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81601 SSF81601, 1 hit
SSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49443-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL
60 70 80 90 100
ETWSFFAVYD GHAGSQVAKY CCEHLLDHIT NNQDFRGSAG APSVENVKNG
110 120 130 140 150
IRTGFLEIDE HMRVMSEKKH GADRSGSTAV GVLISPQHTY FINCGDSRGL
160 170 180 190 200
LCRNRKVHFF TQDHKPSNPL EKERIQNAGG SVMIQRVNGS LAVSRALGDF
210 220 230 240 250
DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG IWDVMGNEEL
260 270 280 290 300
CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPSAPKVSAE
310 320 330 340 350
AVKKEAELDK YLESRVEEII KKQVEGVPDL VHVMRTLASE NIPSLPPGGE
360 370 380
LASKRNVIEA VYNRLNPYKN DDTDSASTDD MW
Length:382
Mass (Da):42,433
Last modified:February 1, 1996 - v1
Checksum:iDC206610E1583870
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28117 mRNA Translation: BAA05662.1
BC008595 mRNA Translation: AAH08595.1
CCDSiCCDS25970.1
PIRiI53823
RefSeqiNP_032936.1, NM_008910.3
XP_006515666.1, XM_006515603.3
UniGeneiMm.261045

Genome annotation databases

EnsembliENSMUST00000021514; ENSMUSP00000021514; ENSMUSG00000021096
GeneIDi19042
KEGGimmu:19042
UCSCiuc007nvu.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPPM1A_MOUSE
AccessioniPrimary (citable) accession number: P49443
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health