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Protein

Protein phosphatase 1A

Gene

Ppm1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling (By similarity). Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+, Mn2+Note: Binds 2 magnesium or manganese ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60Manganese 1By similarity1
Metal bindingi60Manganese 2By similarity1
Metal bindingi61Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi239Manganese 2By similarity1
Metal bindingi282Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2173795. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
R-MMU-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1A (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2C isoform alpha
Short name:
PP2C-alpha
Protein phosphatase IA
Gene namesi
Name:Ppm1a
Synonyms:Pppm1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:99878. Ppm1a.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • membrane Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000577422 – 382Protein phosphatase 1AAdd BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei375PhosphoserineCombined sources1
Modified residuei377PhosphoserineCombined sources1

Post-translational modificationi

N-myristoylation is essential for the recognition of its substrates for dephosphorylation.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiP49443.
MaxQBiP49443.
PaxDbiP49443.
PeptideAtlasiP49443.
PRIDEiP49443.

PTM databases

iPTMnetiP49443.
PhosphoSitePlusiP49443.
SwissPalmiP49443.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021096.
GenevisibleiP49443. MM.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling (By similarity). Interacts with the phosphorylated form of IKBKB/IKKB (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202332. 3 interactors.
IntActiP49443. 2 interactors.
MINTiMINT-4108351.
STRINGi10090.ENSMUSP00000021514.

Structurei

3D structure databases

ProteinModelPortaliP49443.
SMRiP49443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 291PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0697. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00740000115384.
HOGENOMiHOG000233895.
HOVERGENiHBG053647.
InParanoidiP49443.
KOiK04457.
OMAiEPEVYAI.
OrthoDBiEOG091G0AJQ.
PhylomeDBiP49443.
TreeFamiTF313590.

Family and domain databases

Gene3Di1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR012911. PP2C_C.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49443-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL
60 70 80 90 100
ETWSFFAVYD GHAGSQVAKY CCEHLLDHIT NNQDFRGSAG APSVENVKNG
110 120 130 140 150
IRTGFLEIDE HMRVMSEKKH GADRSGSTAV GVLISPQHTY FINCGDSRGL
160 170 180 190 200
LCRNRKVHFF TQDHKPSNPL EKERIQNAGG SVMIQRVNGS LAVSRALGDF
210 220 230 240 250
DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG IWDVMGNEEL
260 270 280 290 300
CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPSAPKVSAE
310 320 330 340 350
AVKKEAELDK YLESRVEEII KKQVEGVPDL VHVMRTLASE NIPSLPPGGE
360 370 380
LASKRNVIEA VYNRLNPYKN DDTDSASTDD MW
Length:382
Mass (Da):42,433
Last modified:February 1, 1996 - v1
Checksum:iDC206610E1583870
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28117 mRNA. Translation: BAA05662.1.
BC008595 mRNA. Translation: AAH08595.1.
CCDSiCCDS25970.1.
PIRiI53823.
RefSeqiNP_032936.1. NM_008910.3.
XP_006515666.1. XM_006515603.3.
UniGeneiMm.261045.

Genome annotation databases

EnsembliENSMUST00000021514; ENSMUSP00000021514; ENSMUSG00000021096.
GeneIDi19042.
KEGGimmu:19042.
UCSCiuc007nvu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28117 mRNA. Translation: BAA05662.1.
BC008595 mRNA. Translation: AAH08595.1.
CCDSiCCDS25970.1.
PIRiI53823.
RefSeqiNP_032936.1. NM_008910.3.
XP_006515666.1. XM_006515603.3.
UniGeneiMm.261045.

3D structure databases

ProteinModelPortaliP49443.
SMRiP49443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202332. 3 interactors.
IntActiP49443. 2 interactors.
MINTiMINT-4108351.
STRINGi10090.ENSMUSP00000021514.

PTM databases

iPTMnetiP49443.
PhosphoSitePlusiP49443.
SwissPalmiP49443.

Proteomic databases

EPDiP49443.
MaxQBiP49443.
PaxDbiP49443.
PeptideAtlasiP49443.
PRIDEiP49443.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021514; ENSMUSP00000021514; ENSMUSG00000021096.
GeneIDi19042.
KEGGimmu:19042.
UCSCiuc007nvu.2. mouse.

Organism-specific databases

CTDi5494.
MGIiMGI:99878. Ppm1a.

Phylogenomic databases

eggNOGiKOG0697. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00740000115384.
HOGENOMiHOG000233895.
HOVERGENiHBG053647.
InParanoidiP49443.
KOiK04457.
OMAiEPEVYAI.
OrthoDBiEOG091G0AJQ.
PhylomeDBiP49443.
TreeFamiTF313590.

Enzyme and pathway databases

ReactomeiR-MMU-2173795. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
R-MMU-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Miscellaneous databases

ChiTaRSiPpm1a. mouse.
PROiP49443.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021096.
GenevisibleiP49443. MM.

Family and domain databases

Gene3Di1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR012911. PP2C_C.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPM1A_MOUSE
AccessioniPrimary (citable) accession number: P49443
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.