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P49435

- APT1_YEAST

UniProt

P49435 - APT1_YEAST

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Protein

Adenine phosphoribosyltransferase 1

Gene

APT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.1 Publication

Catalytic activityi

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi133 – 1375AMPCurated

GO - Molecular functioni

  1. adenine phosphoribosyltransferase activity Source: SGD
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. adenine salvage Source: SGD
  2. AMP biosynthetic process Source: SGD
  3. AMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YML022W-MONOMER.
YEAST:YML022W-MONOMER.
ReactomeiREACT_240981. Purine salvage.
UniPathwayiUPA00588; UER00646.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine phosphoribosyltransferase 1 (EC:2.4.2.7)
Short name:
APRT 1
Gene namesi
Name:APT1
Ordered Locus Names:YML022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML022w.
SGDiS000004484. APT1.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi69 – 691R → A: 4-fold decrease in activity. 1 Publication
Mutagenesisi89 – 891R → A: 2-fold decrease in activity. 1 Publication
Mutagenesisi90 – 901K → A: 30-fold decrease in activity. 1 Publication
Mutagenesisi93 – 931K → A: Small increase in activity. 1 Publication
Mutagenesisi103 – 1031Y → F: 4-fold increase in activity. 1 Publication
Mutagenesisi106 – 1061E → L: 1 million-fold decrease in activity. 1 Publication
Mutagenesisi106 – 1061E → Q: 2-fold decrease in activity. 1 Publication
Mutagenesisi107 – 1071Y → D: 2/3-fold decrease in activity. 1 Publication
Mutagenesisi107 – 1071Y → F: Small decrease in activity. 1 Publication
Mutagenesisi108 – 1081G → A: Small decrease in activity. 1 Publication
Mutagenesisi108 – 1081G → H: 2/3-fold decrease in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 187187Adenine phosphoribosyltransferase 1PRO_0000149517Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei68 – 681Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49435.
PaxDbiP49435.
PeptideAtlasiP49435.

Expressioni

Gene expression databases

GenevestigatoriP49435.

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

BioGridi35147. 26 interactions.
DIPiDIP-2057N.
IntActiP49435. 1 interaction.
MINTiMINT-504301.
STRINGi4932.YML022W.

Structurei

Secondary structure

1
187
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1312Combined sources
Beta strandi15 – 173Combined sources
Beta strandi27 – 293Combined sources
Helixi31 – 355Combined sources
Helixi37 – 5418Combined sources
Beta strandi62 – 665Combined sources
Turni67 – 693Combined sources
Helixi70 – 8112Combined sources
Beta strandi84 – 907Combined sources
Beta strandi96 – 1049Combined sources
Beta strandi109 – 1157Combined sources
Beta strandi124 – 13411Combined sources
Helixi136 – 14712Combined sources
Beta strandi151 – 16010Combined sources
Helixi162 – 1643Combined sources
Helixi166 – 1694Combined sources
Beta strandi174 – 1763Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G2PX-ray1.75A1-187[»]
1G2QX-ray1.50A/B1-187[»]
ProteinModelPortaliP49435.
SMRiP49435. Positions 1-178.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49435.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0503.
GeneTreeiENSGT00390000017259.
HOGENOMiHOG000036776.
InParanoidiP49435.
KOiK00759.
OMAiTELMISH.
OrthoDBiEOG7WT4CC.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr.
InterProiIPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01090. apt. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49435-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSIASYAQEL KLALHQYPNF PSEGILFEDF LPIFRNPGLF QKLIDAFKLH
60 70 80 90 100
LEEAFPEVKI DYIVGLESRG FLFGPTLALA LGVGFVPVRK AGKLPGECFK
110 120 130 140 150
ATYEKEYGSD LFEIQKNAIP AGSNVIIVDD IIATGGSAAA AGELVEQLEA
160 170 180
NLLEYNFVME LDFLKGRSKL NAPVFTLLNA QKEALKK
Length:187
Mass (Da):20,587
Last modified:February 21, 2006 - v3
Checksum:i68061D4BAA42EDEF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371P → F in AAA89075. (PubMed:7642142)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16781 Genomic DNA. Translation: AAA89075.1.
Z46659 Genomic DNA. Translation: CAA86633.1.
AY558232 Genomic DNA. Translation: AAS56558.1.
BK006946 Genomic DNA. Translation: DAA09876.1.
PIRiS49755.
RefSeqiNP_013690.1. NM_001182380.1.

Genome annotation databases

EnsemblFungiiYML022W; YML022W; YML022W.
GeneIDi854986.
KEGGisce:YML022W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16781 Genomic DNA. Translation: AAA89075.1 .
Z46659 Genomic DNA. Translation: CAA86633.1 .
AY558232 Genomic DNA. Translation: AAS56558.1 .
BK006946 Genomic DNA. Translation: DAA09876.1 .
PIRi S49755.
RefSeqi NP_013690.1. NM_001182380.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G2P X-ray 1.75 A 1-187 [» ]
1G2Q X-ray 1.50 A/B 1-187 [» ]
ProteinModelPortali P49435.
SMRi P49435. Positions 1-178.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35147. 26 interactions.
DIPi DIP-2057N.
IntActi P49435. 1 interaction.
MINTi MINT-504301.
STRINGi 4932.YML022W.

Proteomic databases

MaxQBi P49435.
PaxDbi P49435.
PeptideAtlasi P49435.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML022W ; YML022W ; YML022W .
GeneIDi 854986.
KEGGi sce:YML022W.

Organism-specific databases

CYGDi YML022w.
SGDi S000004484. APT1.

Phylogenomic databases

eggNOGi COG0503.
GeneTreei ENSGT00390000017259.
HOGENOMi HOG000036776.
InParanoidi P49435.
KOi K00759.
OMAi TELMISH.
OrthoDBi EOG7WT4CC.

Enzyme and pathway databases

UniPathwayi UPA00588 ; UER00646 .
BioCyci MetaCyc:YML022W-MONOMER.
YEAST:YML022W-MONOMER.
Reactomei REACT_240981. Purine salvage.

Miscellaneous databases

EvolutionaryTracei P49435.
NextBioi 978115.
PROi P49435.

Gene expression databases

Genevestigatori P49435.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
HAMAPi MF_00004. Aden_phosphoribosyltr.
InterProi IPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01090. apt. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the adenine phosphoribosyltransferase-encoding gene (APT1) from Saccharomyces cerevisiae."
    Alfonzo J.D., Sahota A., Deeley M.C., Ranjekar P., Taylor M.W.
    Gene 161:81-85(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DS1.
  2. Taylor M.W., Alfonzo J.D.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Purification and characterization of adenine phosphoribosyltransferase from Saccharomyces cerevisiae."
    Alfonzo J.D., Sahota A., Taylor M.W.
    Biochim. Biophys. Acta 1341:173-182(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR, SUBUNIT.
    Strain: ATCC 24903.
  7. "APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae."
    Alfonzo J.D., Crother T.R., Guetsova M.L., Daignan-Fornier B., Taylor M.W.
    J. Bacteriol. 181:347-352(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Structural analysis of adenine phosphoribosyltransferase from Saccharomyces cerevisiae."
    Shi W., Tanaka K.S.E., Crother T.R., Taylor M.W., Almo S.C., Schramm V.L.
    Biochemistry 40:10800-10809(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 3-187 OF APOENZYME AND IN COMPLEX WITH SUBSTRATE ANALOG, HOMODIMERIZATION, MUTAGENESIS OF ARG-69; ARG-89; LYS-90; LYS-93; TYR-103; GLU-106; TYR-107 AND GLY-108.

Entry informationi

Entry nameiAPT1_YEAST
AccessioniPrimary (citable) accession number: P49435
Secondary accession number(s): D6VZF2, Q6Q5A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 21, 2006
Last modified: November 26, 2014
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3