Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P49435 (APT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenine phosphoribosyltransferase 1

Short name=APRT 1
EC=2.4.2.7
Gene names
Name:APT1
Ordered Locus Names:YML022W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Ref.7

Catalytic activity

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00004

Cofactor

Magnesium. Ref.6

Pathway

Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1. HAMAP-Rule MF_00004

Subunit structure

Homodimer. Ref.6 Ref.7 Ref.11

Subcellular location

Cytoplasm. Nucleus Ref.8.

Miscellaneous

Present with 11200 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 187187Adenine phosphoribosyltransferase 1 HAMAP-Rule MF_00004
PRO_0000149517

Regions

Nucleotide binding133 – 1375AMP Probable

Amino acid modifications

Modified residue681Phosphoserine Ref.10

Experimental info

Mutagenesis691R → A: 4-fold decrease in activity. Ref.11
Mutagenesis891R → A: 2-fold decrease in activity. Ref.11
Mutagenesis901K → A: 30-fold decrease in activity. Ref.11
Mutagenesis931K → A: Small increase in activity. Ref.11
Mutagenesis1031Y → F: 4-fold increase in activity. Ref.11
Mutagenesis1061E → L: 1 million-fold decrease in activity. Ref.11
Mutagenesis1061E → Q: 2-fold decrease in activity. Ref.11
Mutagenesis1071Y → D: 2/3-fold decrease in activity. Ref.11
Mutagenesis1071Y → F: Small decrease in activity. Ref.11
Mutagenesis1081G → A: Small decrease in activity. Ref.11
Mutagenesis1081G → H: 2/3-fold decrease in activity. Ref.11
Sequence conflict371P → F in AAA89075. Ref.1

Secondary structure

................................. 187
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P49435 [UniParc].

Last modified February 21, 2006. Version 3.
Checksum: 68061D4BAA42EDEF

FASTA18720,587
        10         20         30         40         50         60 
MSIASYAQEL KLALHQYPNF PSEGILFEDF LPIFRNPGLF QKLIDAFKLH LEEAFPEVKI 

        70         80         90        100        110        120 
DYIVGLESRG FLFGPTLALA LGVGFVPVRK AGKLPGECFK ATYEKEYGSD LFEIQKNAIP 

       130        140        150        160        170        180 
AGSNVIIVDD IIATGGSAAA AGELVEQLEA NLLEYNFVME LDFLKGRSKL NAPVFTLLNA 


QKEALKK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the adenine phosphoribosyltransferase-encoding gene (APT1) from Saccharomyces cerevisiae."
Alfonzo J.D., Sahota A., Deeley M.C., Ranjekar P., Taylor M.W.
Gene 161:81-85(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DS1.
[2]Taylor M.W., Alfonzo J.D.
Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Purification and characterization of adenine phosphoribosyltransferase from Saccharomyces cerevisiae."
Alfonzo J.D., Sahota A., Taylor M.W.
Biochim. Biophys. Acta 1341:173-182(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: COFACTOR, SUBUNIT.
Strain: ATCC 24903.
[7]"APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae."
Alfonzo J.D., Crother T.R., Guetsova M.L., Daignan-Fornier B., Taylor M.W.
J. Bacteriol. 181:347-352(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Structural analysis of adenine phosphoribosyltransferase from Saccharomyces cerevisiae."
Shi W., Tanaka K.S.E., Crother T.R., Taylor M.W., Almo S.C., Schramm V.L.
Biochemistry 40:10800-10809(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 3-187 OF APOENZYME AND IN COMPLEX WITH SUBSTRATE ANALOG, HOMODIMERIZATION, MUTAGENESIS OF ARG-69; ARG-89; LYS-90; LYS-93; TYR-103; GLU-106; TYR-107 AND GLY-108.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U16781 Genomic DNA. Translation: AAA89075.1.
Z46659 Genomic DNA. Translation: CAA86633.1.
AY558232 Genomic DNA. Translation: AAS56558.1.
BK006946 Genomic DNA. Translation: DAA09876.1.
PIRS49755.
RefSeqNP_013690.1. NM_001182380.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1G2PX-ray1.75A1-187[»]
1G2QX-ray1.50A/B1-187[»]
ProteinModelPortalP49435.
SMRP49435. Positions 1-178.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35147. 25 interactions.
DIPDIP-2057N.
IntActP49435. 1 interaction.
MINTMINT-504301.
STRING4932.YML022W.

Proteomic databases

MaxQBP49435.
PaxDbP49435.
PeptideAtlasP49435.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML022W; YML022W; YML022W.
GeneID854986.
KEGGsce:YML022W.

Organism-specific databases

CYGDYML022w.
SGDS000004484. APT1.

Phylogenomic databases

eggNOGCOG0503.
GeneTreeENSGT00390000017259.
HOGENOMHOG000036776.
KOK00759.
OMATELMISH.
OrthoDBEOG7WT4CC.

Enzyme and pathway databases

BioCycMetaCyc:YML022W-MONOMER.
YEAST:YML022W-MONOMER.
UniPathwayUPA00588; UER00646.

Gene expression databases

GenevestigatorP49435.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
HAMAPMF_00004. Aden_phosphoribosyltr.
InterProIPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMSSF53271. SSF53271. 1 hit.
TIGRFAMsTIGR01090. apt. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP49435.
NextBio978115.
PROP49435.

Entry information

Entry nameAPT1_YEAST
AccessionPrimary (citable) accession number: P49435
Secondary accession number(s): D6VZF2, Q6Q5A3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 21, 2006
Last modified: July 9, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways