Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Adenine phosphoribosyltransferase 1

Gene

APT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.1 Publication

Miscellaneous

Present with 11200 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+1 Publication

Pathwayi: AMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from adenine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenine phosphoribosyltransferase 2 (APT2), Adenine phosphoribosyltransferase 1 (APT1)
This subpathway is part of the pathway AMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from adenine, the pathway AMP biosynthesis via salvage pathway and in Purine metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi133 – 137AMPCurated5

GO - Molecular functioni

  • adenine phosphoribosyltransferase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • adenine salvage Source: SGD
  • AMP biosynthetic process Source: SGD
  • AMP salvage Source: UniProtKB-UniPathway
  • purine ribonucleoside salvage Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YML022W-MONOMER.
YEAST:YML022W-MONOMER.
ReactomeiR-SCE-6798695. Neutrophil degranulation.
R-SCE-74217. Purine salvage.
UniPathwayiUPA00588; UER00646.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine phosphoribosyltransferase 1 (EC:2.4.2.7)
Short name:
APRT 1
Gene namesi
Name:APT1
Ordered Locus Names:YML022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML022W.
SGDiS000004484. APT1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi69R → A: 4-fold decrease in activity. 1 Publication1
Mutagenesisi89R → A: 2-fold decrease in activity. 1 Publication1
Mutagenesisi90K → A: 30-fold decrease in activity. 1 Publication1
Mutagenesisi93K → A: Small increase in activity. 1 Publication1
Mutagenesisi103Y → F: 4-fold increase in activity. 1 Publication1
Mutagenesisi106E → L: 1 million-fold decrease in activity. 1 Publication1
Mutagenesisi106E → Q: 2-fold decrease in activity. 1 Publication1
Mutagenesisi107Y → D: 2/3-fold decrease in activity. 1 Publication1
Mutagenesisi107Y → F: Small decrease in activity. 1 Publication1
Mutagenesisi108G → A: Small decrease in activity. 1 Publication1
Mutagenesisi108G → H: 2/3-fold decrease in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001495171 – 187Adenine phosphoribosyltransferase 1Add BLAST187

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei68PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49435.
PRIDEiP49435.
TopDownProteomicsiP49435.

PTM databases

iPTMnetiP49435.

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

BioGridi35147. 52 interactors.
DIPiDIP-2057N.
IntActiP49435. 2 interactors.
MINTiMINT-504301.
STRINGi4932.YML022W.

Structurei

Secondary structure

1187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 13Combined sources12
Beta strandi15 – 17Combined sources3
Beta strandi27 – 29Combined sources3
Helixi31 – 35Combined sources5
Helixi37 – 54Combined sources18
Beta strandi62 – 66Combined sources5
Turni67 – 69Combined sources3
Helixi70 – 81Combined sources12
Beta strandi84 – 90Combined sources7
Beta strandi96 – 104Combined sources9
Beta strandi109 – 115Combined sources7
Beta strandi124 – 134Combined sources11
Helixi136 – 147Combined sources12
Beta strandi151 – 160Combined sources10
Helixi162 – 164Combined sources3
Helixi166 – 169Combined sources4
Beta strandi174 – 176Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G2PX-ray1.75A1-187[»]
1G2QX-ray1.50A/B1-187[»]
ProteinModelPortaliP49435.
SMRiP49435.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49435.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000017259.
HOGENOMiHOG000036776.
InParanoidiP49435.
KOiK00759.
OMAiCAPIRKK.
OrthoDBiEOG092C4X82.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr. 1 hit.
InterProiView protein in InterPro
IPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
PfamiView protein in Pfam
PF00156. Pribosyltran. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01090. apt. 1 hit.
PROSITEiView protein in PROSITE
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.

Sequencei

Sequence statusi: Complete.

P49435-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIASYAQEL KLALHQYPNF PSEGILFEDF LPIFRNPGLF QKLIDAFKLH
60 70 80 90 100
LEEAFPEVKI DYIVGLESRG FLFGPTLALA LGVGFVPVRK AGKLPGECFK
110 120 130 140 150
ATYEKEYGSD LFEIQKNAIP AGSNVIIVDD IIATGGSAAA AGELVEQLEA
160 170 180
NLLEYNFVME LDFLKGRSKL NAPVFTLLNA QKEALKK
Length:187
Mass (Da):20,587
Last modified:February 21, 2006 - v3
Checksum:i68061D4BAA42EDEF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37P → F in AAA89075 (PubMed:7642142).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16781 Genomic DNA. Translation: AAA89075.1.
Z46659 Genomic DNA. Translation: CAA86633.1.
AY558232 Genomic DNA. Translation: AAS56558.1.
BK006946 Genomic DNA. Translation: DAA09876.1.
PIRiS49755.
RefSeqiNP_013690.1. NM_001182380.1.

Genome annotation databases

EnsemblFungiiYML022W; YML022W; YML022W.
GeneIDi854986.
KEGGisce:YML022W.

Similar proteinsi

Entry informationi

Entry nameiAPT1_YEAST
AccessioniPrimary (citable) accession number: P49435
Secondary accession number(s): D6VZF2, Q6Q5A3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 21, 2006
Last modified: October 25, 2017
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names