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Protein

Adenine phosphoribosyltransferase 1

Gene

APT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.1 Publication

Catalytic activityi

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi133 – 1375AMPCurated

GO - Molecular functioni

  1. adenine phosphoribosyltransferase activity Source: SGD
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. adenine salvage Source: SGD
  2. AMP biosynthetic process Source: SGD
  3. AMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YML022W-MONOMER.
YEAST:YML022W-MONOMER.
ReactomeiREACT_278671. Purine salvage.
UniPathwayiUPA00588; UER00646.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine phosphoribosyltransferase 1 (EC:2.4.2.7)
Short name:
APRT 1
Gene namesi
Name:APT1
Ordered Locus Names:YML022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYML022w.
EuPathDBiFungiDB:YML022W.
SGDiS000004484. APT1.

Subcellular locationi

  1. Cytoplasm 1 Publication
  2. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi69 – 691R → A: 4-fold decrease in activity. 1 Publication
Mutagenesisi89 – 891R → A: 2-fold decrease in activity. 1 Publication
Mutagenesisi90 – 901K → A: 30-fold decrease in activity. 1 Publication
Mutagenesisi93 – 931K → A: Small increase in activity. 1 Publication
Mutagenesisi103 – 1031Y → F: 4-fold increase in activity. 1 Publication
Mutagenesisi106 – 1061E → L: 1 million-fold decrease in activity. 1 Publication
Mutagenesisi106 – 1061E → Q: 2-fold decrease in activity. 1 Publication
Mutagenesisi107 – 1071Y → D: 2/3-fold decrease in activity. 1 Publication
Mutagenesisi107 – 1071Y → F: Small decrease in activity. 1 Publication
Mutagenesisi108 – 1081G → A: Small decrease in activity. 1 Publication
Mutagenesisi108 – 1081G → H: 2/3-fold decrease in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 187187Adenine phosphoribosyltransferase 1PRO_0000149517Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei68 – 681Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49435.
PaxDbiP49435.
PeptideAtlasiP49435.

Expressioni

Gene expression databases

GenevestigatoriP49435.

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

BioGridi35147. 26 interactions.
DIPiDIP-2057N.
IntActiP49435. 1 interaction.
MINTiMINT-504301.
STRINGi4932.YML022W.

Structurei

Secondary structure

1
187
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1312Combined sources
Beta strandi15 – 173Combined sources
Beta strandi27 – 293Combined sources
Helixi31 – 355Combined sources
Helixi37 – 5418Combined sources
Beta strandi62 – 665Combined sources
Turni67 – 693Combined sources
Helixi70 – 8112Combined sources
Beta strandi84 – 907Combined sources
Beta strandi96 – 1049Combined sources
Beta strandi109 – 1157Combined sources
Beta strandi124 – 13411Combined sources
Helixi136 – 14712Combined sources
Beta strandi151 – 16010Combined sources
Helixi162 – 1643Combined sources
Helixi166 – 1694Combined sources
Beta strandi174 – 1763Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G2PX-ray1.75A1-187[»]
1G2QX-ray1.50A/B1-187[»]
ProteinModelPortaliP49435.
SMRiP49435. Positions 1-178.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49435.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0503.
GeneTreeiENSGT00390000017259.
HOGENOMiHOG000036776.
InParanoidiP49435.
KOiK00759.
OMAiIDAFKLH.
OrthoDBiEOG7WT4CC.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr.
InterProiIPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01090. apt. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49435-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIASYAQEL KLALHQYPNF PSEGILFEDF LPIFRNPGLF QKLIDAFKLH
60 70 80 90 100
LEEAFPEVKI DYIVGLESRG FLFGPTLALA LGVGFVPVRK AGKLPGECFK
110 120 130 140 150
ATYEKEYGSD LFEIQKNAIP AGSNVIIVDD IIATGGSAAA AGELVEQLEA
160 170 180
NLLEYNFVME LDFLKGRSKL NAPVFTLLNA QKEALKK
Length:187
Mass (Da):20,587
Last modified:February 21, 2006 - v3
Checksum:i68061D4BAA42EDEF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371P → F in AAA89075 (PubMed:7642142).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16781 Genomic DNA. Translation: AAA89075.1.
Z46659 Genomic DNA. Translation: CAA86633.1.
AY558232 Genomic DNA. Translation: AAS56558.1.
BK006946 Genomic DNA. Translation: DAA09876.1.
PIRiS49755.
RefSeqiNP_013690.1. NM_001182380.1.

Genome annotation databases

EnsemblFungiiYML022W; YML022W; YML022W.
GeneIDi854986.
KEGGisce:YML022W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16781 Genomic DNA. Translation: AAA89075.1.
Z46659 Genomic DNA. Translation: CAA86633.1.
AY558232 Genomic DNA. Translation: AAS56558.1.
BK006946 Genomic DNA. Translation: DAA09876.1.
PIRiS49755.
RefSeqiNP_013690.1. NM_001182380.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G2PX-ray1.75A1-187[»]
1G2QX-ray1.50A/B1-187[»]
ProteinModelPortaliP49435.
SMRiP49435. Positions 1-178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35147. 26 interactions.
DIPiDIP-2057N.
IntActiP49435. 1 interaction.
MINTiMINT-504301.
STRINGi4932.YML022W.

Proteomic databases

MaxQBiP49435.
PaxDbiP49435.
PeptideAtlasiP49435.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML022W; YML022W; YML022W.
GeneIDi854986.
KEGGisce:YML022W.

Organism-specific databases

CYGDiYML022w.
EuPathDBiFungiDB:YML022W.
SGDiS000004484. APT1.

Phylogenomic databases

eggNOGiCOG0503.
GeneTreeiENSGT00390000017259.
HOGENOMiHOG000036776.
InParanoidiP49435.
KOiK00759.
OMAiIDAFKLH.
OrthoDBiEOG7WT4CC.

Enzyme and pathway databases

UniPathwayiUPA00588; UER00646.
BioCyciMetaCyc:YML022W-MONOMER.
YEAST:YML022W-MONOMER.
ReactomeiREACT_278671. Purine salvage.

Miscellaneous databases

EvolutionaryTraceiP49435.
NextBioi978115.
PROiP49435.

Gene expression databases

GenevestigatoriP49435.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr.
InterProiIPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01090. apt. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the adenine phosphoribosyltransferase-encoding gene (APT1) from Saccharomyces cerevisiae."
    Alfonzo J.D., Sahota A., Deeley M.C., Ranjekar P., Taylor M.W.
    Gene 161:81-85(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DS1.
  2. Taylor M.W., Alfonzo J.D.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Purification and characterization of adenine phosphoribosyltransferase from Saccharomyces cerevisiae."
    Alfonzo J.D., Sahota A., Taylor M.W.
    Biochim. Biophys. Acta 1341:173-182(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR, SUBUNIT.
    Strain: ATCC 24903.
  7. "APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae."
    Alfonzo J.D., Crother T.R., Guetsova M.L., Daignan-Fornier B., Taylor M.W.
    J. Bacteriol. 181:347-352(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Structural analysis of adenine phosphoribosyltransferase from Saccharomyces cerevisiae."
    Shi W., Tanaka K.S.E., Crother T.R., Taylor M.W., Almo S.C., Schramm V.L.
    Biochemistry 40:10800-10809(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 3-187 OF APOENZYME AND IN COMPLEX WITH SUBSTRATE ANALOG, HOMODIMERIZATION, MUTAGENESIS OF ARG-69; ARG-89; LYS-90; LYS-93; TYR-103; GLU-106; TYR-107 AND GLY-108.

Entry informationi

Entry nameiAPT1_YEAST
AccessioniPrimary (citable) accession number: P49435
Secondary accession number(s): D6VZF2, Q6Q5A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 21, 2006
Last modified: April 29, 2015
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.