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P49435

- APT1_YEAST

UniProt

P49435 - APT1_YEAST

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Protein
Adenine phosphoribosyltransferase 1
Gene
APT1, YML022W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.1 Publication

Catalytic activityi

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Magnesium.1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi133 – 1375AMP Inferred

GO - Molecular functioni

  1. adenine phosphoribosyltransferase activity Source: SGD
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. AMP biosynthetic process Source: SGD
  2. AMP salvage Source: UniProtKB-UniPathway
  3. adenine salvage Source: SGD
  4. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YML022W-MONOMER.
YEAST:YML022W-MONOMER.
UniPathwayiUPA00588; UER00646.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine phosphoribosyltransferase 1 (EC:2.4.2.7)
Short name:
APRT 1
Gene namesi
Name:APT1
Ordered Locus Names:YML022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML022w.
SGDiS000004484. APT1.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi69 – 691R → A: 4-fold decrease in activity. 1 Publication
Mutagenesisi89 – 891R → A: 2-fold decrease in activity. 1 Publication
Mutagenesisi90 – 901K → A: 30-fold decrease in activity. 1 Publication
Mutagenesisi93 – 931K → A: Small increase in activity. 1 Publication
Mutagenesisi103 – 1031Y → F: 4-fold increase in activity. 1 Publication
Mutagenesisi106 – 1061E → L: 1 million-fold decrease in activity. 1 Publication
Mutagenesisi106 – 1061E → Q: 2-fold decrease in activity. 1 Publication
Mutagenesisi107 – 1071Y → D: 2/3-fold decrease in activity. 1 Publication
Mutagenesisi107 – 1071Y → F: Small decrease in activity. 1 Publication
Mutagenesisi108 – 1081G → A: Small decrease in activity. 1 Publication
Mutagenesisi108 – 1081G → H: 2/3-fold decrease in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 187187Adenine phosphoribosyltransferase 1UniRule annotation
PRO_0000149517Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei68 – 681Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49435.
PaxDbiP49435.
PeptideAtlasiP49435.

Expressioni

Gene expression databases

GenevestigatoriP49435.

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

BioGridi35147. 25 interactions.
DIPiDIP-2057N.
IntActiP49435. 1 interaction.
MINTiMINT-504301.
STRINGi4932.YML022W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1312
Beta strandi15 – 173
Beta strandi27 – 293
Helixi31 – 355
Helixi37 – 5418
Beta strandi62 – 665
Turni67 – 693
Helixi70 – 8112
Beta strandi84 – 907
Beta strandi96 – 1049
Beta strandi109 – 1157
Beta strandi124 – 13411
Helixi136 – 14712
Beta strandi151 – 16010
Helixi162 – 1643
Helixi166 – 1694
Beta strandi174 – 1763

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G2PX-ray1.75A1-187[»]
1G2QX-ray1.50A/B1-187[»]
ProteinModelPortaliP49435.
SMRiP49435. Positions 1-178.

Miscellaneous databases

EvolutionaryTraceiP49435.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0503.
GeneTreeiENSGT00390000017259.
HOGENOMiHOG000036776.
KOiK00759.
OMAiTELMISH.
OrthoDBiEOG7WT4CC.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr.
InterProiIPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01090. apt. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49435-1 [UniParc]FASTAAdd to Basket

« Hide

MSIASYAQEL KLALHQYPNF PSEGILFEDF LPIFRNPGLF QKLIDAFKLH    50
LEEAFPEVKI DYIVGLESRG FLFGPTLALA LGVGFVPVRK AGKLPGECFK 100
ATYEKEYGSD LFEIQKNAIP AGSNVIIVDD IIATGGSAAA AGELVEQLEA 150
NLLEYNFVME LDFLKGRSKL NAPVFTLLNA QKEALKK 187
Length:187
Mass (Da):20,587
Last modified:February 21, 2006 - v3
Checksum:i68061D4BAA42EDEF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371P → F in AAA89075. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U16781 Genomic DNA. Translation: AAA89075.1.
Z46659 Genomic DNA. Translation: CAA86633.1.
AY558232 Genomic DNA. Translation: AAS56558.1.
BK006946 Genomic DNA. Translation: DAA09876.1.
PIRiS49755.
RefSeqiNP_013690.1. NM_001182380.1.

Genome annotation databases

EnsemblFungiiYML022W; YML022W; YML022W.
GeneIDi854986.
KEGGisce:YML022W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U16781 Genomic DNA. Translation: AAA89075.1 .
Z46659 Genomic DNA. Translation: CAA86633.1 .
AY558232 Genomic DNA. Translation: AAS56558.1 .
BK006946 Genomic DNA. Translation: DAA09876.1 .
PIRi S49755.
RefSeqi NP_013690.1. NM_001182380.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G2P X-ray 1.75 A 1-187 [» ]
1G2Q X-ray 1.50 A/B 1-187 [» ]
ProteinModelPortali P49435.
SMRi P49435. Positions 1-178.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35147. 25 interactions.
DIPi DIP-2057N.
IntActi P49435. 1 interaction.
MINTi MINT-504301.
STRINGi 4932.YML022W.

Proteomic databases

MaxQBi P49435.
PaxDbi P49435.
PeptideAtlasi P49435.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML022W ; YML022W ; YML022W .
GeneIDi 854986.
KEGGi sce:YML022W.

Organism-specific databases

CYGDi YML022w.
SGDi S000004484. APT1.

Phylogenomic databases

eggNOGi COG0503.
GeneTreei ENSGT00390000017259.
HOGENOMi HOG000036776.
KOi K00759.
OMAi TELMISH.
OrthoDBi EOG7WT4CC.

Enzyme and pathway databases

UniPathwayi UPA00588 ; UER00646 .
BioCyci MetaCyc:YML022W-MONOMER.
YEAST:YML022W-MONOMER.

Miscellaneous databases

EvolutionaryTracei P49435.
NextBioi 978115.
PROi P49435.

Gene expression databases

Genevestigatori P49435.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
HAMAPi MF_00004. Aden_phosphoribosyltr.
InterProi IPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01090. apt. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the adenine phosphoribosyltransferase-encoding gene (APT1) from Saccharomyces cerevisiae."
    Alfonzo J.D., Sahota A., Deeley M.C., Ranjekar P., Taylor M.W.
    Gene 161:81-85(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DS1.
  2. Taylor M.W., Alfonzo J.D.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Purification and characterization of adenine phosphoribosyltransferase from Saccharomyces cerevisiae."
    Alfonzo J.D., Sahota A., Taylor M.W.
    Biochim. Biophys. Acta 1341:173-182(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR, SUBUNIT.
    Strain: ATCC 24903.
  7. "APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae."
    Alfonzo J.D., Crother T.R., Guetsova M.L., Daignan-Fornier B., Taylor M.W.
    J. Bacteriol. 181:347-352(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Structural analysis of adenine phosphoribosyltransferase from Saccharomyces cerevisiae."
    Shi W., Tanaka K.S.E., Crother T.R., Taylor M.W., Almo S.C., Schramm V.L.
    Biochemistry 40:10800-10809(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 3-187 OF APOENZYME AND IN COMPLEX WITH SUBSTRATE ANALOG, HOMODIMERIZATION, MUTAGENESIS OF ARG-69; ARG-89; LYS-90; LYS-93; TYR-103; GLU-106; TYR-107 AND GLY-108.

Entry informationi

Entry nameiAPT1_YEAST
AccessioniPrimary (citable) accession number: P49435
Secondary accession number(s): D6VZF2, Q6Q5A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 21, 2006
Last modified: July 9, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11200 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3