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P49435

- APT1_YEAST

UniProt

P49435 - APT1_YEAST

Protein

Adenine phosphoribosyltransferase 1

Gene

APT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 3 (21 Feb 2006)
      Previous versions | rss
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    Functioni

    Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.1 Publication

    Catalytic activityi

    AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Magnesium.1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi133 – 1375AMPCurated

    GO - Molecular functioni

    1. adenine phosphoribosyltransferase activity Source: SGD
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. adenine salvage Source: SGD
    2. AMP biosynthetic process Source: SGD
    3. AMP salvage Source: UniProtKB-UniPathway
    4. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:YML022W-MONOMER.
    YEAST:YML022W-MONOMER.
    UniPathwayiUPA00588; UER00646.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenine phosphoribosyltransferase 1 (EC:2.4.2.7)
    Short name:
    APRT 1
    Gene namesi
    Name:APT1
    Ordered Locus Names:YML022W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML022w.
    SGDiS000004484. APT1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi69 – 691R → A: 4-fold decrease in activity. 1 Publication
    Mutagenesisi89 – 891R → A: 2-fold decrease in activity. 1 Publication
    Mutagenesisi90 – 901K → A: 30-fold decrease in activity. 1 Publication
    Mutagenesisi93 – 931K → A: Small increase in activity. 1 Publication
    Mutagenesisi103 – 1031Y → F: 4-fold increase in activity. 1 Publication
    Mutagenesisi106 – 1061E → L: 1 million-fold decrease in activity. 1 Publication
    Mutagenesisi106 – 1061E → Q: 2-fold decrease in activity. 1 Publication
    Mutagenesisi107 – 1071Y → D: 2/3-fold decrease in activity. 1 Publication
    Mutagenesisi107 – 1071Y → F: Small decrease in activity. 1 Publication
    Mutagenesisi108 – 1081G → A: Small decrease in activity. 1 Publication
    Mutagenesisi108 – 1081G → H: 2/3-fold decrease in activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 187187Adenine phosphoribosyltransferase 1PRO_0000149517Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei68 – 681Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP49435.
    PaxDbiP49435.
    PeptideAtlasiP49435.

    Expressioni

    Gene expression databases

    GenevestigatoriP49435.

    Interactioni

    Subunit structurei

    Homodimer.3 Publications

    Protein-protein interaction databases

    BioGridi35147. 25 interactions.
    DIPiDIP-2057N.
    IntActiP49435. 1 interaction.
    MINTiMINT-504301.
    STRINGi4932.YML022W.

    Structurei

    Secondary structure

    1
    187
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 1312
    Beta strandi15 – 173
    Beta strandi27 – 293
    Helixi31 – 355
    Helixi37 – 5418
    Beta strandi62 – 665
    Turni67 – 693
    Helixi70 – 8112
    Beta strandi84 – 907
    Beta strandi96 – 1049
    Beta strandi109 – 1157
    Beta strandi124 – 13411
    Helixi136 – 14712
    Beta strandi151 – 16010
    Helixi162 – 1643
    Helixi166 – 1694
    Beta strandi174 – 1763

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G2PX-ray1.75A1-187[»]
    1G2QX-ray1.50A/B1-187[»]
    ProteinModelPortaliP49435.
    SMRiP49435. Positions 1-178.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP49435.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0503.
    GeneTreeiENSGT00390000017259.
    HOGENOMiHOG000036776.
    KOiK00759.
    OMAiTELMISH.
    OrthoDBiEOG7WT4CC.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    HAMAPiMF_00004. Aden_phosphoribosyltr.
    InterProiIPR005764. Ade_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01090. apt. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P49435-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSIASYAQEL KLALHQYPNF PSEGILFEDF LPIFRNPGLF QKLIDAFKLH    50
    LEEAFPEVKI DYIVGLESRG FLFGPTLALA LGVGFVPVRK AGKLPGECFK 100
    ATYEKEYGSD LFEIQKNAIP AGSNVIIVDD IIATGGSAAA AGELVEQLEA 150
    NLLEYNFVME LDFLKGRSKL NAPVFTLLNA QKEALKK 187
    Length:187
    Mass (Da):20,587
    Last modified:February 21, 2006 - v3
    Checksum:i68061D4BAA42EDEF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti37 – 371P → F in AAA89075. (PubMed:7642142)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16781 Genomic DNA. Translation: AAA89075.1.
    Z46659 Genomic DNA. Translation: CAA86633.1.
    AY558232 Genomic DNA. Translation: AAS56558.1.
    BK006946 Genomic DNA. Translation: DAA09876.1.
    PIRiS49755.
    RefSeqiNP_013690.1. NM_001182380.1.

    Genome annotation databases

    EnsemblFungiiYML022W; YML022W; YML022W.
    GeneIDi854986.
    KEGGisce:YML022W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16781 Genomic DNA. Translation: AAA89075.1 .
    Z46659 Genomic DNA. Translation: CAA86633.1 .
    AY558232 Genomic DNA. Translation: AAS56558.1 .
    BK006946 Genomic DNA. Translation: DAA09876.1 .
    PIRi S49755.
    RefSeqi NP_013690.1. NM_001182380.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1G2P X-ray 1.75 A 1-187 [» ]
    1G2Q X-ray 1.50 A/B 1-187 [» ]
    ProteinModelPortali P49435.
    SMRi P49435. Positions 1-178.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35147. 25 interactions.
    DIPi DIP-2057N.
    IntActi P49435. 1 interaction.
    MINTi MINT-504301.
    STRINGi 4932.YML022W.

    Proteomic databases

    MaxQBi P49435.
    PaxDbi P49435.
    PeptideAtlasi P49435.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML022W ; YML022W ; YML022W .
    GeneIDi 854986.
    KEGGi sce:YML022W.

    Organism-specific databases

    CYGDi YML022w.
    SGDi S000004484. APT1.

    Phylogenomic databases

    eggNOGi COG0503.
    GeneTreei ENSGT00390000017259.
    HOGENOMi HOG000036776.
    KOi K00759.
    OMAi TELMISH.
    OrthoDBi EOG7WT4CC.

    Enzyme and pathway databases

    UniPathwayi UPA00588 ; UER00646 .
    BioCyci MetaCyc:YML022W-MONOMER.
    YEAST:YML022W-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P49435.
    NextBioi 978115.
    PROi P49435.

    Gene expression databases

    Genevestigatori P49435.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    HAMAPi MF_00004. Aden_phosphoribosyltr.
    InterProi IPR005764. Ade_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01090. apt. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the adenine phosphoribosyltransferase-encoding gene (APT1) from Saccharomyces cerevisiae."
      Alfonzo J.D., Sahota A., Deeley M.C., Ranjekar P., Taylor M.W.
      Gene 161:81-85(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DS1.
    2. Taylor M.W., Alfonzo J.D.
      Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Purification and characterization of adenine phosphoribosyltransferase from Saccharomyces cerevisiae."
      Alfonzo J.D., Sahota A., Taylor M.W.
      Biochim. Biophys. Acta 1341:173-182(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: COFACTOR, SUBUNIT.
      Strain: ATCC 24903.
    7. "APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae."
      Alfonzo J.D., Crother T.R., Guetsova M.L., Daignan-Fornier B., Taylor M.W.
      J. Bacteriol. 181:347-352(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Structural analysis of adenine phosphoribosyltransferase from Saccharomyces cerevisiae."
      Shi W., Tanaka K.S.E., Crother T.R., Taylor M.W., Almo S.C., Schramm V.L.
      Biochemistry 40:10800-10809(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 3-187 OF APOENZYME AND IN COMPLEX WITH SUBSTRATE ANALOG, HOMODIMERIZATION, MUTAGENESIS OF ARG-69; ARG-89; LYS-90; LYS-93; TYR-103; GLU-106; TYR-107 AND GLY-108.

    Entry informationi

    Entry nameiAPT1_YEAST
    AccessioniPrimary (citable) accession number: P49435
    Secondary accession number(s): D6VZF2, Q6Q5A3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 21, 2006
    Last modified: October 1, 2014
    This is version 134 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 11200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3