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P49434

- PYRF_PICST

UniProt

P49434 - PYRF_PICST

Protein

Orotidine 5'-phosphate decarboxylase

Gene

URA3

Organism
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Orotidine 5'-phosphate = UMP + CO2.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei38 – 381SubstrateBy similarity
    Active sitei94 – 941Proton donorPROSITE-ProRule annotation
    Binding sitei218 – 2181SubstrateBy similarity
    Binding sitei236 – 2361SubstrateBy similarity

    GO - Molecular functioni

    1. orotidine-5'-phosphate decarboxylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
    2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Pyrimidine biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00070; UER00120.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
    Alternative name(s):
    OMP decarboxylase
    Short name:
    OMPDCase
    Short name:
    OMPdecase
    Uridine 5'-monophosphate synthase
    Short name:
    UMP synthase
    Gene namesi
    Name:URA3
    ORF Names:PICST_50417
    OrganismiScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
    Taxonomic identifieri322104 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeScheffersomyces
    ProteomesiUP000002258: Chromosome 8

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 267267Orotidine 5'-phosphate decarboxylasePRO_0000134675Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi4924.PICST_50417.

    Structurei

    3D structure databases

    ProteinModelPortaliP49434.
    SMRiP49434. Positions 6-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni60 – 623Substrate bindingBy similarity
    Regioni92 – 10110Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the OMP decarboxylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0284.
    HOGENOMiHOG000213905.
    KOiK01591.
    OMAiMGQQYRT.
    OrthoDBiEOG7CVQ76.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR014732. OMPdecase.
    IPR018089. OMPdecase_AS.
    IPR001754. OMPdeCOase_dom.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view]
    PfamiPF00215. OMPdecase. 1 hit.
    [Graphical view]
    SMARTiSM00934. OMPdecase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51366. SSF51366. 1 hit.
    TIGRFAMsiTIGR01740. pyrF. 1 hit.
    PROSITEiPS00156. OMPDECASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P49434-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVNVQTYTQR AESHQSPVAQ RLFKLMDSKK TNLCASVDVK TTSEFLSLID    50
    KLGPYICLVK THIDIIDDFS YEGTIVPLLE LSKKHNFMIF EDRKFADIGN 100
    TVKSQYSGGV YKIAQWSDIT NAHGVTGSGI VQGLKEAARE TTDEPRGLLM 150
    LAELSSKGSI AHGKYTEETV EIAKTDKEFV IGFIAQRDMG GQDEGFDWIV 200
    MTPGVGLDDK GDSLGQQYRT VDEVVSTGTD IIIVGRGLFG KGRDPDVEGQ 250
    RYREAGWNAY LKKTSQV 267
    Length:267
    Mass (Da):29,529
    Last modified:February 1, 1996 - v1
    Checksum:iD98361C398F49E06
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08629 Genomic DNA. Translation: AAA65978.1.
    CP000502 Genomic DNA. Translation: ABN68626.1.
    RefSeqiXP_001386655.1. XM_001386618.1.

    Genome annotation databases

    GeneIDi4840866.
    KEGGipic:PICST_50417.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08629 Genomic DNA. Translation: AAA65978.1 .
    CP000502 Genomic DNA. Translation: ABN68626.1 .
    RefSeqi XP_001386655.1. XM_001386618.1.

    3D structure databases

    ProteinModelPortali P49434.
    SMRi P49434. Positions 6-266.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 4924.PICST_50417.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4840866.
    KEGGi pic:PICST_50417.

    Phylogenomic databases

    eggNOGi COG0284.
    HOGENOMi HOG000213905.
    KOi K01591.
    OMAi MGQQYRT.
    OrthoDBi EOG7CVQ76.

    Enzyme and pathway databases

    UniPathwayi UPA00070 ; UER00120 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR014732. OMPdecase.
    IPR018089. OMPdecase_AS.
    IPR001754. OMPdeCOase_dom.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view ]
    Pfami PF00215. OMPdecase. 1 hit.
    [Graphical view ]
    SMARTi SM00934. OMPdecase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51366. SSF51366. 1 hit.
    TIGRFAMsi TIGR01740. pyrF. 1 hit.
    PROSITEi PS00156. OMPDECASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2."
      Yang V.W., Marks J.A., Davis B.P., Jeffries T.W.
      Appl. Environ. Microbiol. 60:4245-4254(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545.

    Entry informationi

    Entry nameiPYRF_PICST
    AccessioniPrimary (citable) accession number: P49434
    Secondary accession number(s): A3M000
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3