Reviewed, UniProtKB/Swiss-Prot P49433 (G3P1_SYNY3)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 1
      Short name=GAPDH 1
      Short name=GAP-1
    EC=1.2.1.12

Gene names

Name:	gap1
Ordered Locus Names:	slr0884

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Glyceraldehyde-3-phosphate dehydrogenase 1		PRO_0000145708
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	152 – 154	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	212 – 213	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	153	1	Nucleophile By similarity
	Binding site	33	1	NAD By similarity
	Binding site	77	1	NAD; via carbonyl oxygen By similarity
	Binding site	183	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	235	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	317	1	NAD By similarity
	Site	180	1	Activates thiol group during catalysis By similarity
Experimental info
	Sequence conflict	78	1	N → D in BAA17609. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P49433-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 4C97358AD070B1E6	FASTA	339	36,146
	10 20 30 40 50 60 MLKIGINGFG RIGRLVARIA MANPQVTLVG INDLVPASNL AYLFKYDSTH GSYGGTVVAK 70 80 90 100 110 120 EEGIVIDDQF IPCFSQRNPA QLPWGDLGAD YVVESTGLFT TYATAENHLK AGAKRVIISA 130 140 150 160 170 180 PSKDPEKIPT FVVGVNHLNY NADTDKIVSN ASCTTNCLAP IAKILDDNFG IVEGLMTTVH 190 200 210 220 230 240 AMTATQPTVD GPSKKDFRGG RGAAQNIIPS STGAAKAAAL VLPQLKGKLT GMAFRVPTPN 250 260 270 280 290 300 VSVVDLTFKT EKATSYEEIC AAMKTAAEGE LKGILGYTAD DVVSMDFRTD PRSSIFDAGA 310 320 330 GIGLNSNFFK VVSWYDNEWG YSCRVIDLML TMASKDGLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Schubert M., Brinkmann H., Cerff R. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X86375 Genomic DNA. Translation: CAA60134.1. BA000022 Genomic DNA. Translation: BAA17609.1. Different initiation.
PIR	S77275.
RefSeq	NP_440929.1.
3D structure databases
HSSP	HSSP built from PDB template 1CRW based on UniProtKB P56649.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49433.
Genome annotation databases
GeneID	954234.
GenomeReviews	Gene locus slr0884 in contig BA000022_GR.
KEGG	syn:slr0884.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P49433.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0884-MON.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P1_SYNY3

Accession

Primary (citable) accession number: P49433
Secondary accession number(s): P73569

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 25, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)