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Protein

Pyruvate dehydrogenase E1 component subunit beta, mitochondrial

Gene

Pdhb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.By similarity

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89Thiamine pyrophosphateBy similarity1
Sitei319Important for interaction with DLATBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism, Tricarboxylic acid cycle
LigandPyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

ReactomeiR-RNO-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-RNO-389661 Glyoxylate metabolism and glycine degradation
R-RNO-5362517 Signaling by Retinoic Acid
R-RNO-70268 Pyruvate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (EC:1.2.4.1)
Short name:
PDHE1-B
Gene namesi
Name:Pdhb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi1359146 Pdhb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30Mitochondrion1 PublicationAdd BLAST30
ChainiPRO_000002045931 – 359Pyruvate dehydrogenase E1 component subunit beta, mitochondrialAdd BLAST329

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67PhosphotyrosineBy similarity1
Modified residuei354N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP49432
PRIDEiP49432

2D gel databases

UCD-2DPAGEiP49432
World-2DPAGEi0004:P49432

PTM databases

CarbonylDBiP49432
iPTMnetiP49432
PhosphoSitePlusiP49432

Expressioni

Gene expression databases

BgeeiENSRNOG00000007895
ExpressionAtlasiP49432 baseline and differential
GenevisibleiP49432 RN

Interactioni

Subunit structurei

Heterotetramer of two PDHA1 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules. Interacts with DLAT.By similarity

Protein-protein interaction databases

BioGridi253064, 1 interactor
ComplexPortaliCPX-378 Pyruvate dehydrogenase E1 heterotetramer
IntActiP49432, 2 interactors
MINTiP49432
STRINGi10116.ENSRNOP00000010545

Structurei

3D structure databases

ProteinModelPortaliP49432
SMRiP49432
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0524 Eukaryota
COG0022 LUCA
GeneTreeiENSGT00530000063423
HOGENOMiHOG000281450
HOVERGENiHBG000917
InParanoidiP49432
KOiK00162
PhylomeDBiP49432
TreeFamiTF105674

Family and domain databases

Gene3Di3.40.50.920, 1 hit
InterProiView protein in InterPro
IPR029061 THDP-binding
IPR009014 Transketo_C/PFOR_II
IPR005475 Transketolase-like_Pyr-bd
IPR033248 Transketolase_C
PfamiView protein in Pfam
PF02779 Transket_pyr, 1 hit
PF02780 Transketolase_C, 1 hit
SMARTiView protein in SMART
SM00861 Transket_pyr, 1 hit
SUPFAMiSSF52518 SSF52518, 1 hit
SSF52922 SSF52922, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVAGLVRG PLRQASGLLK RRFHRSAPAA VQLTVREAIN QGMDEELERD
60 70 80 90 100
EKVFLLGEEV AQYDGAYKVS RGLWKKYGDK RIIDTPISEM GFAGIAVGAA
110 120 130 140 150
MAGLRPICEF MTFNFSMQAI DQVINSAAKT YYMSAGLQPV PIVFRGPNGA
160 170 180 190 200
SAGVAAQHSQ CFAAWYGHCP GLKVVSPWNS EDAKGLIKSA IRDDNPVVML
210 220 230 240 250
ENELMYGVAF ELPTEAQSKD FLIPIGKAKI ERQGTHITVV AHSRPVGHCL
260 270 280 290 300
EAAAVLSKEG IECEVINLRT IRPMDIEAIE ASVMKTNHLV TVEGGWPQFG
310 320 330 340 350
VGAEICARIM EGPAFNFLDA PAVRVTGADV PMPYAKILED NSIPQVKDII

FAIKKTLNI
Length:359
Mass (Da):38,982
Last modified:January 9, 2007 - v2
Checksum:i1B942D0A68C86D51
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3A → G (PubMed:2025639).Curated1
Sequence conflicti11 – 15PLRQA → LCGRL (PubMed:2025639).Curated5
Sequence conflicti22R → L (PubMed:2025639).Curated1
Sequence conflicti25R → C (PubMed:2025639).Curated1
Sequence conflicti238T → N (PubMed:2025639).Curated1
Sequence conflicti241 – 242AH → CY (PubMed:2025639).Curated2
Sequence conflicti259E → G (PubMed:2025639).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079137 mRNA Translation: AAH79137.1
PIRiS15892
RefSeqiNP_001007621.1, NM_001007620.1
XP_006251816.1, XM_006251754.1
UniGeneiRn.102424

Genome annotation databases

EnsembliENSRNOT00000010545; ENSRNOP00000010545; ENSRNOG00000007895
GeneIDi289950
KEGGirno:289950

Similar proteinsi

Entry informationi

Entry nameiODPB_RAT
AccessioniPrimary (citable) accession number: P49432
Secondary accession number(s): Q6AY95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 9, 2007
Last modified: June 20, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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