P49431 (SAT1_MUSSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diamine acetyltransferase 1
EC=2.3.1.57

Alternative name(s):
Polyamine N-acetyltransferase 1
Putrescine acetyltransferase
Spermidine/spermine N(1)-acetyltransferase 1
Short name=SSAT
Short name=SSAT-1

Gene names

Name:	Sat1
Synonyms:	Sat

Organism

Mus saxicola (Brown spiny mouse) (Rock-loving mouse)

Taxonomic identifier

10094 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Pyromys

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine By similarity.
Catalytic activity	Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.
Pathway	Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the acetyltransferase family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Diamine acetyltransferase 1		PRO_0000074594
Regions
	Domain	4 – 170	167	N-acetyltransferase
	Region	27 – 28	2	Substrate binding By similarity
	Region	94 – 96	3	Acetyl-CoA binding By similarity
	Region	102 – 107	6	Acetyl-CoA binding By similarity
	Region	126 – 128	3	Substrate binding By similarity
	Region	133 – 136	4	Acetyl-CoA binding By similarity
	Region	140 – 143	4	Acetyl-CoA binding By similarity
Sites
	Binding site	92	1	Substrate; via carbonyl oxygen By similarity
	Binding site	152	1	Substrate By similarity
Amino acid modifications
	Modified residue	26	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49431 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 30125A9F7AA81888	FASTA	171	19,997
	10 20 30 40 50 60 MAKFKIRPAT ASDCSDILRL IKELAKYEYM EDQVILTEKD LLEDGFGEHP FYHCLVAEVP 70 80 90 100 110 120 KEHWTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY RGFGIGSEIL KNLSQVAMKC 130 140 150 160 170 RCSSMHFLVA EWNEPSINFY KRRGASDLSS EEGWRLFKID KEYLLKMAAE E

References

[1]

"Cloning and sequence analysis of the gene and cDNA encoding mouse spermidine/spermine N1-acetyltransferase -- a gene uniquely regulated by polyamines and their analogs." Fogel-Petrovic M., Kramer D.L., Ganis B., Casero R.A. Jr., Porter C.W. Biochim. Biophys. Acta 1216:255-264(1993) [PubMed: 8241266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L10245 Unassigned DNA. Translation: AAA16567.1.
PIR	S43430.
3D structure databases
ProteinModelPortal	P49431.
SMR	P49431. Positions 2-171.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:98233. Sat1.
Phylogenomic databases
HOVERGEN	HBG063175.
Family and domain databases
InterPro	IPR000182. AcTrfase_GCN5-related_dom. IPR016181. Acyl_CoA_acyltransferase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAT1_MUSSA

Accession

Primary (citable) accession number: P49431

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 31, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program