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P49428

- UBCX_PICPA

UniProt

P49428 - UBCX_PICPA

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Protein

Ubiquitin-conjugating enzyme E2-24 kDa

Gene

PEX4

Organism
Komagataella pastoris (Yeast) (Pichia pastoris)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis.

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei133 – 1331Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. peroxisome organization Source: UniProtKB-KW
  2. protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Peroxisome biogenesis, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-24 kDa (EC:6.3.2.19)
Alternative name(s):
Peroxin-4
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:PEX4
Synonyms:PAS4
OrganismiKomagataella pastoris (Yeast) (Pichia pastoris)
Taxonomic identifieri4922 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeKomagataella

Subcellular locationi

GO - Cellular componenti

  1. peroxisome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi133 – 1331C → S or A: Loss of activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Ubiquitin-conjugating enzyme E2-24 kDaPRO_0000082567Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP49428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.10.110.10. 2 hits.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 2 hits.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49428-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAEKRLLQE YRSILKEQRQ KGSASTLSSN GILDLKPVSE DNFYKWTAKL
60 70 80 90 100
KGPTDTGYQD AFWELQIDIP SNYPTQPPKF TFIVSDDIPR NRRQRQTNQI
110 120 130 140 150
QDDDEFEGAE KEVLRHCYRM PHPNIAFNTG EICLDILQAK WTPAWTLSSA
160 170 180 190 200
LTAIVLLLND PEPLSPLDID MANLMKINDL KAYNSLIEYY VGRYSIEEEV

YILN
Length:204
Mass (Da):23,566
Last modified:February 1, 1996 - v1
Checksum:i46680ABD73121F6C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12511 Genomic DNA. Translation: AAA53634.1.
PIRiA53848.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12511 Genomic DNA. Translation: AAA53634.1 .
PIRi A53848.

3D structure databases

ProteinModelPortali P49428.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00143 .

Family and domain databases

Gene3Di 3.10.110.10. 2 hits.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 2 hits.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Pichia pastoris PAS4 gene encodes a ubiquitin-conjugating enzyme required for peroxisome assembly."
    Crane D.I., Kalish J.E., Gould S.J.
    J. Biol. Chem. 269:21835-21844(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiUBCX_PICPA
AccessioniPrimary (citable) accession number: P49428
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3