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P49428 (UBCX_PICPA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2-24 kDa

EC=6.3.2.19
Alternative name(s):
Peroxin-4
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene names
Name:PEX4
Synonyms:PAS4
OrganismKomagataella pastoris (Yeast) (Pichia pastoris)
Taxonomic identifier4922 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKomagataella

Protein attributes

Sequence length204 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subcellular location

Peroxisome.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processPeroxisome biogenesis
Ubl conjugation pathway
   Cellular componentPeroxisome
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processperoxisome organization

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentperoxisome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 204204Ubiquitin-conjugating enzyme E2-24 kDa
PRO_0000082567

Sites

Active site1331Glycyl thioester intermediate By similarity

Experimental info

Mutagenesis1331C → S or A: Loss of activity.

Sequences

Sequence LengthMass (Da)Tools
P49428 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 46680ABD73121F6C

FASTA20423,566
        10         20         30         40         50         60 
MSAEKRLLQE YRSILKEQRQ KGSASTLSSN GILDLKPVSE DNFYKWTAKL KGPTDTGYQD 

        70         80         90        100        110        120 
AFWELQIDIP SNYPTQPPKF TFIVSDDIPR NRRQRQTNQI QDDDEFEGAE KEVLRHCYRM 

       130        140        150        160        170        180 
PHPNIAFNTG EICLDILQAK WTPAWTLSSA LTAIVLLLND PEPLSPLDID MANLMKINDL 

       190        200 
KAYNSLIEYY VGRYSIEEEV YILN 

« Hide

References

[1]"The Pichia pastoris PAS4 gene encodes a ubiquitin-conjugating enzyme required for peroxisome assembly."
Crane D.I., Kalish J.E., Gould S.J.
J. Biol. Chem. 269:21835-21844(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12511 Genomic DNA. Translation: AAA53634.1.
PIRA53848.

3D structure databases

ProteinModelPortalP49428.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 2 hits.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 2 hits.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBCX_PICPA
AccessionPrimary (citable) accession number: P49428
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 16, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways