P49426 (EXG1_COCCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucan 1,3-beta-glucosidase
EC=3.2.1.58

Alternative name(s):
1,3-beta-D-glucanohydrolase
Exo-beta 1,3 glucanase

Gene names

Name:

EXG1

Organism

Cochliobolus carbonum (Bipolaris zeicola)

Taxonomic identifier

5017 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Pleosporaceae › Cochliobolus

Protein attributes

Sequence length	788 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 55 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucan exo-1,3-beta-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 42	42
	Chain	43 – 788	746	Glucan 1,3-beta-glucosidase		PRO_0000012226
Amino acid modifications
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Glycosylation	233	1	N-linked (GlcNAc...) Potential
	Glycosylation	381	1	N-linked (GlcNAc...) Potential
	Glycosylation	773	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P49426 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: DD202BC4400DE750	FASTA	788	84,013
	10 20 30 40 50 60 MRFSSLLACL GAVGIQAAAI PFQRRVDNTT DSGSLDAAQA AAAIVDGYWL NDLSGKGRAP 70 80 90 100 110 120 FNSNPNYKVF RNVKDYGAKG DGVTDDSDAF NRAISDGSRC GPWVCDSSTD SPAVVYVPSG 130 140 150 160 170 180 TYLINKPIIF YYMTALIGNP RELPVLKAAS SLQALALIDG SPYSNQNGEP GWISTNLFLR 190 200 210 220 230 240 QIRNLIIDGT AVAPTSGFQA IHWPASQATT IQNVKIRMTQ ASNSVHAGIF VENGSGGHMA 250 260 270 280 290 300 DLDITGGLYG MNIGNQQFTM RNVKISKAVV GISQIWNWGW LYSGLQISDC GTAFSMVNGG 310 320 330 340 350 360 SAGKQEVGSA VIIDSEITNC QKFVDSAWSQ TSNPTGSGQL VIENIKLTNV PAAVVSNGAT 370 380 390 400 410 420 VLAGGSLTIQ TWGQGNKYAP NASGPSKFQG AISGATRPTG LLQNGKFYSK SKPQYETLST 430 440 450 460 470 480 SSFISARGAG ATGDGVTDDT RAVQAAVTQA ASQNKVLFFE HGVYKVTNTI YVPPGSRMVG 490 500 510 520 530 540 EIFSAIMGSG STFGDQANPV PIIQIGKPGE SGSIEWSDMI VQTQGATPGA IVIQYNLNTA 550 560 570 580 590 600 LGSGLWDVHT RIGGAKGTNL QVAQCPAVLG QVKPECFSAH TNVHVTKGAN GAYFENNWFW 610 620 630 640 650 660 TADHDLDDAD STRINIYTGR GFHVEANNVW IWANGAEHHT MYQYQFNAAQ DIFAGYIQTE 670 680 690 700 710 720 TPYFQPTPIA PLPYVSSSKY SDPVYSSSQT SAWGLRLLDA KNVLIYGGGL YSFFDNYDVG 730 740 750 760 770 780 CSSPTAPNGF RDCQTRILSI EGSTSVQAFG FSEVGVEWMV TAAGQDKANW KDNLSVYPTT IGYLSYGF

References

[1]	"Cloning and targeted gene disruption of EXG1, encoding exo-beta 1, 3-glucanase, in the phytopathogenic fungus Cochliobolus carbonum." Schaeffer H.J., Leykam J., Walton J.D. Appl. Environ. Microbiol. 60:594-598(1994) [PubMed: 8135518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 90305 / SB111 / 2R15.
[2]	"A single beta 1,3-glucanase secreted by the maize pathogen Cochliobolus carbonum acts by an exolytic mechanism." van Hoof A., Leykam J., Schaeffer H.J., Walton J.D. Physiol. Mol. Plant Pathol. 39:259-267(1991) [Agricola: IND92032302] Cited for: PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L48994 Genomic DNA. Translation: AAC71062.1.
3D structure databases
ProteinModelPortal	P49426.
ModBase	Search...
Protein family/group databases
CAZy	GH55. Glycoside Hydrolase Family 55.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 3 hits.
SUPFAM	SSF51126. Pectin_lyas_like. 2 hits.
ProtoNet	Search...

Entry information

Entry name

EXG1_COCCA

Accession

Primary (citable) accession number: P49426

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 31, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program