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P49425

- MANA_RHOM4

UniProt

P49425 - MANA_RHOM4

Protein

Mannan endo-1,4-beta-mannosidase

Gene

manA

Organism
Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) (Rhodothermus obamensis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 3 (19 Jan 2010)
      Previous versions | rss
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    Functioni

    Acts as endo-acting enzyme with a requirement for at least five sugar moieties for effective catalytic activity. Hydrolyzes carob-galactomannan (locust bean gum) effectively and to a smaller extent guar gum, but not yeast mannan.1 Publication

    Catalytic activityi

    Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

    pH dependencei

    Optimum pH is 5.4.1 Publication

    Temperature dependencei

    Optimum temperature is 85 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. cellulase activity Source: InterPro
    3. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. substituted mannan metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciRMAR518766:GJJ8-18-MONOMER.
    BRENDAi3.2.1.78. 5425.

    Protein family/group databases

    CAZyiCBM35. Carbohydrate-Binding Module Family 35.
    GH26. Glycoside Hydrolase Family 26.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannan endo-1,4-beta-mannosidase (EC:3.2.1.78)
    Alternative name(s):
    Endo-(1,4)-beta-mannanase
    Gene namesi
    Name:manA
    Ordered Locus Names:Rmar_0016
    OrganismiRhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) (Rhodothermus obamensis)
    Taxonomic identifieri518766 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeRhodothermus
    ProteomesiUP000002221: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 558558Mannan endo-1,4-beta-mannosidasePRO_0000057685Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi518766.Rmar_0016.

    Structurei

    3D structure databases

    ProteinModelPortaliP49425.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini17 – 137121CBM6PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 26 family.Curated
    Contains 1 CBM6 (carbohydrate binding type-6) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG4124.
    HOGENOMiHOG000235025.
    OrthoDBiEOG66HVDV.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR005084. CMB_fam6.
    IPR022790. EndoGluc_H/Glyco_hydro_26.
    IPR008979. Galactose-bd-like.
    IPR000805. Glyco_hydro_26.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR026444. Secre_tail.
    [Graphical view]
    PfamiPF02156. Glyco_hydro_26. 1 hit.
    [Graphical view]
    PRINTSiPR00739. GLHYDRLASE26.
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR04183. Por_Secre_tail. 1 hit.
    PROSITEiPS51175. CBM6. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P49425-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLLLVWLIF TGVAGEIRLE AEDGELLGVA VDSTLTGYSG RGYVTGFDAP    50
    EDSVRFSFEA PRGVYRVVFG VSFSSRFASY ALRVDDWHQT GSLIKRGGGF 100
    FEASIGEIWL DEGAHTMAFQ LMNGALDYVR LEPVSYGPPA RPPAQLSDSQ 150
    ATASAQALFA FLLSEYGRHI LAGQQQNPYR RDFDAINYVR NVTGKEPALV 200
    SFDLIDYSPT REAHGVVHYQ TPEDWIAWAG RDGIVSLMWH WNAPTDLIED 250
    PSQDCYWWYG FYTRCTTFDV AAALADTSSE RYRLLLRDID VIAAQLQKFQ 300
    QADIPVLWRP LHEAAGGWFW WGAKGPEPFK QLWRLLYERL VHHHGLHNLI 350
    WVYTHEPGAA EWYPGDAYVD IVGRDVYADD PDALMRSDWN ELQTLFGGRK 400
    LVALTETGTL PDVEVITDYG IWWSWFSIWT DPFLRDVDPD RLTRVYHSER 450
    VLTRDELPDW RSYVLHATTV QPAGDLALAV YPNPGAGRLH VEVGLPVAAP 500
    VVVEVFNLLG QRVFQYQAGM QPAGLWRRAF ELALAPGVYL VQVRAGNLVA 550
    RRRWVSVR 558
    Length:558
    Mass (Da):63,120
    Last modified:January 19, 2010 - v3
    Checksum:iE977F92CEE320807
    GO

    Sequence cautioni

    The sequence CAA62442.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90947 Genomic DNA. Translation: CAA62442.1. Different initiation.
    CP001807 Genomic DNA. Translation: ACY46925.1.
    PIRiT10748.
    RefSeqiYP_003289313.1. NC_013501.1.

    Genome annotation databases

    EnsemblBacteriaiACY46925; ACY46925; Rmar_0016.
    GeneIDi8566638.
    KEGGirmr:Rmar_0016.
    PATRICi32314039. VBIRhoMar93821_0016.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90947 Genomic DNA. Translation: CAA62442.1 . Different initiation.
    CP001807 Genomic DNA. Translation: ACY46925.1 .
    PIRi T10748.
    RefSeqi YP_003289313.1. NC_013501.1.

    3D structure databases

    ProteinModelPortali P49425.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 518766.Rmar_0016.

    Protein family/group databases

    CAZyi CBM35. Carbohydrate-Binding Module Family 35.
    GH26. Glycoside Hydrolase Family 26.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACY46925 ; ACY46925 ; Rmar_0016 .
    GeneIDi 8566638.
    KEGGi rmr:Rmar_0016.
    PATRICi 32314039. VBIRhoMar93821_0016.

    Phylogenomic databases

    eggNOGi COG4124.
    HOGENOMi HOG000235025.
    OrthoDBi EOG66HVDV.

    Enzyme and pathway databases

    BioCyci RMAR518766:GJJ8-18-MONOMER.
    BRENDAi 3.2.1.78. 5425.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR005084. CMB_fam6.
    IPR022790. EndoGluc_H/Glyco_hydro_26.
    IPR008979. Galactose-bd-like.
    IPR000805. Glyco_hydro_26.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR026444. Secre_tail.
    [Graphical view ]
    Pfami PF02156. Glyco_hydro_26. 1 hit.
    [Graphical view ]
    PRINTSi PR00739. GLHYDRLASE26.
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    TIGRFAMsi TIGR04183. Por_Secre_tail. 1 hit.
    PROSITEi PS51175. CBM6. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A highly thermostable endo-(1,4)-beta-mannanase from the marine bacterium Rhodothermus marinus."
      Politz O., Krah M., Thomsen K.K., Borriss R.
      Appl. Microbiol. Biotechnol. 53:715-721(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43812 / DSM 4252 / R-10.

    Entry informationi

    Entry nameiMANA_RHOM4
    AccessioniPrimary (citable) accession number: P49425
    Secondary accession number(s): D0MK12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 19, 2010
    Last modified: October 1, 2014
    This is version 74 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3