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P49424

- MANA_CELJU

UniProt

P49424 - MANA_CELJU

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Protein
Mannan endo-1,4-beta-mannosidase
Gene
manA, man26A, CJA_2770
Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides.2 Publications

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.1 Publication

pH dependencei

Optimum pH is about 7.0.

GO - Molecular functioni

  1. cellulase activity Source: InterPro
  2. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. substituted mannan metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciCJAP498211:GHIT-2761-MONOMER.

Protein family/group databases

CAZyiGH26. Glycoside Hydrolase Family 26.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase (EC:3.2.1.78)
Alternative name(s):
Mannanase A
Gene namesi
Name:manA
Synonyms:man26A
Ordered Locus Names:CJA_2770
OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Taxonomic identifieri498211 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio
ProteomesiUP000001036: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131 Reviewed prediction
Add
BLAST
Propeptidei32 – 387 Reviewed prediction
PRO_0000012172
Chaini39 – 423385Mannan endo-1,4-beta-mannosidase
PRO_0000012173Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi498211.CJA_2770.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi45 – 473
Helixi56 – 6813
Turni69 – 713
Beta strandi73 – 786
Turni79 – 824
Beta strandi91 – 933
Helixi96 – 1016
Beta strandi106 – 1116
Helixi112 – 1143
Helixi125 – 1339
Beta strandi137 – 1415
Turni147 – 1493
Helixi150 – 1523
Turni156 – 1594
Turni169 – 1713
Helixi178 – 19316
Beta strandi205 – 2084
Beta strandi215 – 2184
Turni222 – 2243
Helixi227 – 24216
Beta strandi249 – 2546
Helixi264 – 2685
Turni274 – 2763
Beta strandi278 – 2803
Beta strandi283 – 2864
Beta strandi288 – 2903
Helixi292 – 31221
Beta strandi320 – 3234
Helixi325 – 3295
Helixi337 – 34711
Helixi351 – 3533
Beta strandi355 – 3595
Helixi385 – 3895
Helixi392 – 40110
Helixi408 – 4103
Beta strandi419 – 4213

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GVYX-ray1.70A39-363[»]
1GW1X-ray1.65A43-421[»]
1J9YX-ray1.85A39-423[»]
1ODZX-ray1.40A/B39-423[»]
1R7OX-ray1.85A29-423[»]
2WHMX-ray1.50A39-423[»]
ProteinModelPortaliP49424.
SMRiP49424. Positions 42-422.

Miscellaneous databases

EvolutionaryTraceiP49424.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000274384.
OMAiIANWANE.
OrthoDBiEOG6V4G9N.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR022790. EndoGluc_H/Glyco_hydro_26.
IPR000805. Glyco_hydro_26.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02156. Glyco_hydro_26. 1 hit.
[Graphical view]
PRINTSiPR00739. GLHYDRLASE26.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49424-1 [UniParc]FASTAAdd to Basket

« Hide

MKTITTARLP WAAQSFALGI CLIALLGCNH AANKSSASRA DVKPVTVKLV    50
DSQATMETRS LFAFMQEQRR HSIMFGHQHE TTQGLTITRT DGTQSDTFNA 100
VGDFAAVYGW DTLSIVAPKA EGDIVAQVKK AYARGGIITV SSHFDNPKTD 150
TQKGVWPVGT SWDQTPAVVD SLPGGAYNPV LNGYLDQVAE WANNLKDEQG 200
RLIPVIFRLY HENTGSWFWW GDKQSTPEQY KQLFRYSVEY LRDVKGVRNF 250
LYAYSPNNFW DVTEANYLER YPGDEWVDVL GFDTYGPVAD NADWFRNVVA 300
NAALVARMAE ARGKIPVISE IGIRAPDIEA GLYDNQWYRK LISGLKADPD 350
AREIAFLLVW RNAPQGVPGP NGTQVPHYWV PANRPENINN GTLEDFQAFY 400
ADEFTAFNRD IEQVYQRPTL IVK 423
Length:423
Mass (Da):47,487
Last modified:July 5, 2005 - v2
Checksum:iEFDAB597F10BA3DD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82179 Genomic DNA. Translation: CAA57670.2.
CP000934 Genomic DNA. Translation: ACE82849.1.
PIRiS53374.
RefSeqiWP_012488364.1. NC_010995.1.
YP_001983229.1. NC_010995.1.

Genome annotation databases

EnsemblBacteriaiACE82849; ACE82849; CJA_2770.
GeneIDi6414457.
KEGGicja:CJA_2770.
PATRICi21328934. VBICelJap122165_2735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82179 Genomic DNA. Translation: CAA57670.2 .
CP000934 Genomic DNA. Translation: ACE82849.1 .
PIRi S53374.
RefSeqi WP_012488364.1. NC_010995.1.
YP_001983229.1. NC_010995.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GVY X-ray 1.70 A 39-363 [» ]
1GW1 X-ray 1.65 A 43-421 [» ]
1J9Y X-ray 1.85 A 39-423 [» ]
1ODZ X-ray 1.40 A/B 39-423 [» ]
1R7O X-ray 1.85 A 29-423 [» ]
2WHM X-ray 1.50 A 39-423 [» ]
ProteinModelPortali P49424.
SMRi P49424. Positions 42-422.
ModBasei Search...

Protein-protein interaction databases

STRINGi 498211.CJA_2770.

Protein family/group databases

CAZyi GH26. Glycoside Hydrolase Family 26.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACE82849 ; ACE82849 ; CJA_2770 .
GeneIDi 6414457.
KEGGi cja:CJA_2770.
PATRICi 21328934. VBICelJap122165_2735.

Phylogenomic databases

HOGENOMi HOG000274384.
OMAi IANWANE.
OrthoDBi EOG6V4G9N.

Enzyme and pathway databases

BioCyci CJAP498211:GHIT-2761-MONOMER.

Miscellaneous databases

EvolutionaryTracei P49424.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR022790. EndoGluc_H/Glyco_hydro_26.
IPR000805. Glyco_hydro_26.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02156. Glyco_hydro_26. 1 hit.
[Graphical view ]
PRINTSi PR00739. GLHYDRLASE26.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A non-modular endo-beta-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa."
    Braithwaite K.L., Black G.W., Hazlewood G.P., Ali B.R.S., Gilbert H.J.
    Biochem. J. 305:1005-1010(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 39-48, FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, PH DEPENDENCE.
  2. Gilbert H.J.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 364-423.
  3. "Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
    DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
    J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ueda107.
  4. "alpha-Galactosidase A from Pseudomonas fluorescens subsp. cellulosa: cloning, high level expression and its role in galactomannan hydrolysis."
    Halstead J.R., Fransen M.P., Eberhart R.Y., Park A.J., Gilbert H.J., Hazlewood G.P.
    FEMS Microbiol. Lett. 192:197-203(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMANA_CELJU
AccessioniPrimary (citable) accession number: P49424
Secondary accession number(s): B3PBK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 5, 2005
Last modified: September 3, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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