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P49424

- MANA_CELJU

UniProt

P49424 - MANA_CELJU

Protein

Mannan endo-1,4-beta-mannosidase

Gene

manA

Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides.2 Publications

    Catalytic activityi

    Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.1 Publication

    pH dependencei

    Optimum pH is about 7.0.1 Publication

    GO - Molecular functioni

    1. cellulase activity Source: InterPro
    2. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. substituted mannan metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciCJAP498211:GHIT-2761-MONOMER.

    Protein family/group databases

    CAZyiGH26. Glycoside Hydrolase Family 26.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannan endo-1,4-beta-mannosidase (EC:3.2.1.78)
    Alternative name(s):
    Mannanase A
    Gene namesi
    Name:manA
    Synonyms:man26A
    Ordered Locus Names:CJA_2770
    OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
    Taxonomic identifieri498211 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio
    ProteomesiUP000001036: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Propeptidei32 – 387Sequence AnalysisPRO_0000012172
    Chaini39 – 423385Mannan endo-1,4-beta-mannosidasePRO_0000012173Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi498211.CJA_2770.

    Structurei

    Secondary structure

    1
    423
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi45 – 473
    Helixi56 – 6813
    Turni69 – 713
    Beta strandi73 – 786
    Turni79 – 824
    Beta strandi91 – 933
    Helixi96 – 1016
    Beta strandi106 – 1116
    Helixi112 – 1143
    Helixi125 – 1339
    Beta strandi137 – 1415
    Turni147 – 1493
    Helixi150 – 1523
    Turni156 – 1594
    Turni169 – 1713
    Helixi178 – 19316
    Beta strandi205 – 2084
    Beta strandi215 – 2184
    Turni222 – 2243
    Helixi227 – 24216
    Beta strandi249 – 2546
    Helixi264 – 2685
    Turni274 – 2763
    Beta strandi278 – 2803
    Beta strandi283 – 2864
    Beta strandi288 – 2903
    Helixi292 – 31221
    Beta strandi320 – 3234
    Helixi325 – 3295
    Helixi337 – 34711
    Helixi351 – 3533
    Beta strandi355 – 3595
    Helixi385 – 3895
    Helixi392 – 40110
    Helixi408 – 4103
    Beta strandi419 – 4213

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GVYX-ray1.70A39-363[»]
    1GW1X-ray1.65A43-421[»]
    1J9YX-ray1.85A39-423[»]
    1ODZX-ray1.40A/B39-423[»]
    1R7OX-ray1.85A29-423[»]
    2WHMX-ray1.50A39-423[»]
    ProteinModelPortaliP49424.
    SMRiP49424. Positions 42-422.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP49424.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 26 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000274384.
    OMAiIANWANE.
    OrthoDBiEOG6V4G9N.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR022790. EndoGluc_H/Glyco_hydro_26.
    IPR000805. Glyco_hydro_26.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02156. Glyco_hydro_26. 1 hit.
    [Graphical view]
    PRINTSiPR00739. GLHYDRLASE26.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49424-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTITTARLP WAAQSFALGI CLIALLGCNH AANKSSASRA DVKPVTVKLV    50
    DSQATMETRS LFAFMQEQRR HSIMFGHQHE TTQGLTITRT DGTQSDTFNA 100
    VGDFAAVYGW DTLSIVAPKA EGDIVAQVKK AYARGGIITV SSHFDNPKTD 150
    TQKGVWPVGT SWDQTPAVVD SLPGGAYNPV LNGYLDQVAE WANNLKDEQG 200
    RLIPVIFRLY HENTGSWFWW GDKQSTPEQY KQLFRYSVEY LRDVKGVRNF 250
    LYAYSPNNFW DVTEANYLER YPGDEWVDVL GFDTYGPVAD NADWFRNVVA 300
    NAALVARMAE ARGKIPVISE IGIRAPDIEA GLYDNQWYRK LISGLKADPD 350
    AREIAFLLVW RNAPQGVPGP NGTQVPHYWV PANRPENINN GTLEDFQAFY 400
    ADEFTAFNRD IEQVYQRPTL IVK 423
    Length:423
    Mass (Da):47,487
    Last modified:July 5, 2005 - v2
    Checksum:iEFDAB597F10BA3DD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82179 Genomic DNA. Translation: CAA57670.2.
    CP000934 Genomic DNA. Translation: ACE82849.1.
    PIRiS53374.
    RefSeqiWP_012488364.1. NC_010995.1.
    YP_001983229.1. NC_010995.1.

    Genome annotation databases

    EnsemblBacteriaiACE82849; ACE82849; CJA_2770.
    GeneIDi6414457.
    KEGGicja:CJA_2770.
    PATRICi21328934. VBICelJap122165_2735.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82179 Genomic DNA. Translation: CAA57670.2 .
    CP000934 Genomic DNA. Translation: ACE82849.1 .
    PIRi S53374.
    RefSeqi WP_012488364.1. NC_010995.1.
    YP_001983229.1. NC_010995.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GVY X-ray 1.70 A 39-363 [» ]
    1GW1 X-ray 1.65 A 43-421 [» ]
    1J9Y X-ray 1.85 A 39-423 [» ]
    1ODZ X-ray 1.40 A/B 39-423 [» ]
    1R7O X-ray 1.85 A 29-423 [» ]
    2WHM X-ray 1.50 A 39-423 [» ]
    ProteinModelPortali P49424.
    SMRi P49424. Positions 42-422.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 498211.CJA_2770.

    Protein family/group databases

    CAZyi GH26. Glycoside Hydrolase Family 26.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACE82849 ; ACE82849 ; CJA_2770 .
    GeneIDi 6414457.
    KEGGi cja:CJA_2770.
    PATRICi 21328934. VBICelJap122165_2735.

    Phylogenomic databases

    HOGENOMi HOG000274384.
    OMAi IANWANE.
    OrthoDBi EOG6V4G9N.

    Enzyme and pathway databases

    BioCyci CJAP498211:GHIT-2761-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P49424.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR022790. EndoGluc_H/Glyco_hydro_26.
    IPR000805. Glyco_hydro_26.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02156. Glyco_hydro_26. 1 hit.
    [Graphical view ]
    PRINTSi PR00739. GLHYDRLASE26.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A non-modular endo-beta-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa."
      Braithwaite K.L., Black G.W., Hazlewood G.P., Ali B.R.S., Gilbert H.J.
      Biochem. J. 305:1005-1010(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 39-48, FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, PH DEPENDENCE.
    2. Gilbert H.J.
      Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 364-423.
    3. "Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
      DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
      J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ueda107.
    4. "alpha-Galactosidase A from Pseudomonas fluorescens subsp. cellulosa: cloning, high level expression and its role in galactomannan hydrolysis."
      Halstead J.R., Fransen M.P., Eberhart R.Y., Park A.J., Gilbert H.J., Hazlewood G.P.
      FEMS Microbiol. Lett. 192:197-203(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMANA_CELJU
    AccessioniPrimary (citable) accession number: P49424
    Secondary accession number(s): B3PBK3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3