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Reviewed, UniProtKB/Swiss-Prot P49424 (MANA_PSEFL)

Last modified June 16, 2009. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Mannan endo-1,4-beta-mannosidase
    EC=3.2.1.78
Alternative name(s):
    Mannanase A
Gene names
Name: manA
OrganismPseudomonas fluorescens
Taxonomic identifier294 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Preferentially hydrolyzes the larger oligosaccharides.

Catalytic activity

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sequence similarities

Belongs to the glycosyl hydrolase 26 family.

biophysicochemical properties

pH dependence:

Optimum pH is about 7.0.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Propeptide32 – 387 Potential
PRO_0000012172
Chain39 – 423385Mannan endo-1,4-beta-mannosidase
PRO_0000012173

Secondary structure

............................................................... 423
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P49424-1 [UniParc].

Last modified July 5, 2005. Version 2.
Checksum: EFDAB597F10BA3DD

FASTA42347,487
        10         20         30         40         50         60 
MKTITTARLP WAAQSFALGI CLIALLGCNH AANKSSASRA DVKPVTVKLV DSQATMETRS 

        70         80         90        100        110        120 
LFAFMQEQRR HSIMFGHQHE TTQGLTITRT DGTQSDTFNA VGDFAAVYGW DTLSIVAPKA 

       130        140        150        160        170        180 
EGDIVAQVKK AYARGGIITV SSHFDNPKTD TQKGVWPVGT SWDQTPAVVD SLPGGAYNPV 

       190        200        210        220        230        240 
LNGYLDQVAE WANNLKDEQG RLIPVIFRLY HENTGSWFWW GDKQSTPEQY KQLFRYSVEY 

       250        260        270        280        290        300 
LRDVKGVRNF LYAYSPNNFW DVTEANYLER YPGDEWVDVL GFDTYGPVAD NADWFRNVVA 

       310        320        330        340        350        360 
NAALVARMAE ARGKIPVISE IGIRAPDIEA GLYDNQWYRK LISGLKADPD AREIAFLLVW 

       370        380        390        400        410        420 
RNAPQGVPGP NGTQVPHYWV PANRPENINN GTLEDFQAFY ADEFTAFNRD IEQVYQRPTL 


IVK 

« Hide

References

[1]"A non-modular endo-beta-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa."
Braithwaite K.L., Black G.W., Hazlewood G.P., Ali B.R.S., Gilbert H.J.
Biochem. J. 305:1005-1010(1995) [PubMed: 7848261] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 39-48.
Strain: Sp. Cellulosa.
[2]Gilbert H.J.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 364-423.
+Additional computationally mapped references.

Cross-references

Sequence databases

X82179 Genomic DNA. Translation: CAA57670.2.
PIRS53374.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1GVYX-ray1.70A39-421[»]
1GW1X-ray1.65A43-421[»]
1J9YX-ray1.85A39-423[»]
1ODZX-ray1.40A/B39-423[»]
1R7OX-ray1.85A29-423[»]
ModBaseSearch...

Protein family/group databases

CAZyGH26. Glycoside Hydrolase Family 26.

Enzyme and pathway databases

BRENDA3.2.1.78. 329.

Family and domain databases

InterProIPR000805. Glyco_hydro_26.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF02156. Glyco_hydro_26. 1 hit.
[Graphical view]
PRINTSPR00739. GLHYDRLASE26.
ProtoNetSearch...

Entry information

Entry nameMANA_PSEFL
AccessionPrimary (citable) accession number: P49424
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 5, 2005
Last modified: June 16, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents