Reviewed, UniProtKB/Swiss-Prot P49420 (DHAS_PROMA)

Names and origin

Protein names

Recommended name:
    Aspartate-semialdehyde dehydrogenase
      Short name=ASA dehydrogenase
      Short name=ASADH
    EC=1.2.1.11

Gene names

Name:	asd
Ordered Locus Names:	Pro_1814

Organism

Prochlorococcus marinus [Complete proteome] [HAMAP]

Taxonomic identifier

1219 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 2/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Aspartate-semialdehyde dehydrogenase		PRO_0000141386
Sites
	Active site	137	1	Acyl-thioester intermediate By similarity
Experimental info
	Sequence conflict	238	1	T → P in CAA92210. Ref.2
	Sequence conflict	244 – 246	3	VPV → GPA in CAA92210. Ref.2
	Sequence conflict	253 – 254	2	SI → AT in CAA92210. Ref.2
	Sequence conflict	257 – 260	4	EFAE → VFSG in CAA92210. Ref.2
	Sequence conflict	269 – 270	2	KI → QN in CAA92210. Ref.2
	Sequence conflict	286	1	N → D in CAA92210. Ref.2
	Sequence conflict	297	1	K → Q in CAA92210. Ref.2
	Sequence conflict	305	1	I → K in CAA92210. Ref.2
	Sequence conflict	324	1	I → K in CAA92210. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P49420-1 [UniParc]. Last modified September 26, 2003. Version 2. Checksum: E20036130DB7403B	FASTA	343	37,557
	10 20 30 40 50 60 MTLQKPFPDR PLTLAVLGSS GAVGAEILKI LEERSFPIRE LRLLASERSA GQVQFFKGED 70 80 90 100 110 120 LVVKKVSPEG FEDVDLVLAS AGGSISRKWR KVINSAGAVI VDNSNAYRME PDVPLVVPEV 130 140 150 160 170 180 NPSQVFTHKG LIANPNCTTI LLALVLAPLS AQLPIKRVVV STYQSASGAG ARAMNELKQL 190 200 210 220 230 240 SQDVLNGNIP KSEILPYSLA FNLFLHNSPL QSNNYCEEEM KMINETRKIL NQSELAITAT 250 260 270 280 290 300 CVRVPVLRAH SESINIEFAE PFPVEEARKI LSNASGIKLL EDIQMNRFPM PIDVTGKDDI 310 320 330 340 AVGRIRQDLS NPKALELWLC GDQIRKGAAL NAIQIAELLL TRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome." Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I.M., Barbe V., Duprat S., Galperin M.Y., Koonin E.V., Le Gall F., Makarova K.S., Ostrowski M., Oztas S., Robert C., Rogozin I.B., Scanlan D.J., Tandeau de Marsac N., Weissenbach J. , Wincker P., Wolf Y.I., Hess W.R. Proc. Natl. Acad. Sci. U.S.A. 100:10020-10025(2003) [PubMed: 12917486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SARG / CCMP1375 / SS120.
[2]	"Molecular cloning and characterization of a dihydrodipicolinate synthase (DHDPS) gene from the photoautotrophic prokaryote Prochlorococcus marinus CCMP 1375 (Prochlorophyta)." Lorenz M., Boerner T., Hess W.R. Endocyt. Cell Res. 11:59-68(1995) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 238-343. Strain: SARG / CCMP1375 / SS120.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017126 Genomic DNA. Translation: AAQ00858.1. Z68126 Genomic DNA. Translation: CAA92210.1.
RefSeq	NP_876205.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1463197.
GenomeReviews	Gene locus Pro_1814 in contig AE017126_GR.
KEGG	pma:Pro1814.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P49420.
OMA	P49420. LELWLCG.
Enzyme and pathway databases
BioCyc	PMAR167539:PRO_1814-MON.
BRENDA	1.2.1.11. 141456.
Family and domain databases
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR012080. Asp_semialdehyde_DH. IPR005986. Asp_semialdehyde_DH_bac. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
TIGRFAMs	TIGR01296. asd_B. 1 hit.
PROSITE	PS01103. ASD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_PROMA

Accession

Primary (citable) accession number: P49420

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	September 26, 2003
Last modified:	June 16, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)