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Protein

Elongation factor Tu, mitochondrial

Gene

TUFM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi64 – 71GTPBy similarity8
Nucleotide bindingi126 – 130GTPBy similarity5
Nucleotide bindingi181 – 184GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • RNA binding Source: UniProtKB
  • translation elongation factor activity Source: UniProtKB

GO - Biological processi

  • response to ethanol Source: Ensembl
  • translational elongation Source: UniProtKB

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-5389840. Mitochondrial translation elongation.
SignaLinkiP49411.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Tu, mitochondrial
Short name:
EF-Tu
Alternative name(s):
P43
Gene namesi
Name:TUFM
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000178952.8.
HGNCiHGNC:12420. TUFM.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Involvement in diseasei

Combined oxidative phosphorylation deficiency 4 (COXPD4)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes.
See also OMIM:610678
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031902336R → Q in COXPD4. 1 Publication1

Keywords - Diseasei

Disease mutation, Primary mitochondrial disease

Organism-specific databases

DisGeNETi7284.
MalaCardsiTUFM.
MIMi610678. phenotype.
Orphaneti254925. Combined oxidative phosphorylation defect type 4.
PharmGKBiPA37082.

Chemistry databases

DrugBankiDB04315. Guanosine-5'-Diphosphate.

Polymorphism and mutation databases

BioMutaiTUFM.
DMDMi1706611.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43Mitochondrion2 PublicationsAdd BLAST43
ChainiPRO_000000746244 – 452Elongation factor Tu, mitochondrialAdd BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79N6-acetyllysineCombined sources1
Modified residuei88N6-acetyllysine; alternateCombined sources1
Modified residuei88N6-succinyllysine; alternateBy similarity1
Modified residuei234N6-succinyllysineBy similarity1
Modified residuei256N6-acetyllysineCombined sources1
Modified residuei278PhosphothreonineCombined sources1
Modified residuei286N6-succinyllysineBy similarity1
Modified residuei312PhosphoserineBy similarity1
Modified residuei361N6-acetyllysineBy similarity1
Modified residuei418N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP49411.
MaxQBiP49411.
PaxDbiP49411.
PeptideAtlasiP49411.
PRIDEiP49411.
TopDownProteomicsiP49411.

2D gel databases

DOSAC-COBS-2DPAGEiP49411.
OGPiP49411.
REPRODUCTION-2DPAGEiIPI00027107.
SWISS-2DPAGEiP49411.
UCD-2DPAGEiP49411.

PTM databases

iPTMnetiP49411.
PhosphoSitePlusiP49411.
SwissPalmiP49411.

Expressioni

Gene expression databases

BgeeiENSG00000178952.
CleanExiHS_TUFM.
ExpressionAtlasiP49411. baseline and differential.
GenevisibleiP49411. HS.

Organism-specific databases

HPAiCAB075730.
CAB075731.
HPA018991.
HPA024087.

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi113135. 176 interactors.
CORUMiP49411.
IntActiP49411. 53 interactors.
MINTiMINT-224570.
STRINGi9606.ENSP00000322439.

Structurei

3D structure databases

ProteinModelPortaliP49411.
SMRiP49411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 251tr-type GPROSITE-ProRule annotationAdd BLAST197

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 71G1PROSITE-ProRule annotation8
Regioni105 – 109G2PROSITE-ProRule annotation5
Regioni126 – 129G3PROSITE-ProRule annotation4
Regioni181 – 184G4PROSITE-ProRule annotation4
Regioni219 – 221G5PROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0460. Eukaryota.
COG0050. LUCA.
HOGENOMiHOG000229290.
HOVERGENiHBG001535.
InParanoidiP49411.
KOiK02358.
OrthoDBiEOG091G0BFD.
PhylomeDBiP49411.
TreeFamiTF300432.

Family and domain databases

CDDicd03697. EFTU_II. 1 hit.
InterProiView protein in InterPro
IPR004161. EFTu-like_2.
IPR033720. EFTU_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
PfamiView protein in Pfam
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
PROSITEiView protein in PROSITE
PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAATLLRAT PHFSGLAAGR TFLLQGLLRL LKAPALPLLC RGLAVEAKKT
60 70 80 90 100
YVRDKPHVNV GTIGHVDHGK TTLTAAITKI LAEGGGAKFK KYEEIDNAPE
110 120 130 140 150
ERARGITINA AHVEYSTAAR HYAHTDCPGH ADYVKNMITG TAPLDGCILV
160 170 180 190 200
VAANDGPMPQ TREHLLLARQ IGVEHVVVYV NKADAVQDSE MVELVELEIR
210 220 230 240 250
ELLTEFGYKG EETPVIVGSA LCALEGRDPE LGLKSVQKLL DAVDTYIPVP
260 270 280 290 300
ARDLEKPFLL PVEAVYSVPG RGTVVTGTLE RGILKKGDEC ELLGHSKNIR
310 320 330 340 350
TVVTGIEMFH KSLERAEAGD NLGALVRGLK REDLRRGLVM VKPGSIKPHQ
360 370 380 390 400
KVEAQVYILS KEEGGRHKPF VSHFMPVMFS LTWDMACRII LPPEKELAMP
410 420 430 440 450
GEDLKFNLIL RQPMILEKGQ RFTLRDGNRT IGTGLVTNTL AMTEEEKNIK

WG
Length:452
Mass (Da):49,542
Last modified:October 1, 1996 - v2
Checksum:iE37274ABFFDB5FC7
GO

Sequence cautioni

The sequence AAC60647 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH01633 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH10041 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195 – 197Missing in AAC60647 (PubMed:7828719).Curated3
Sequence conflicti384D → N in AAB00499 (PubMed:8547323).Curated1
Sequence conflicti384D → N in CAA72493 (Ref. 9) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031902336R → Q in COXPD4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38995 mRNA. Translation: AAB00499.1.
S75463 mRNA. Translation: AAC60647.1. Different initiation.
X84694 mRNA. Translation: CAA59169.1.
AC133550 Genomic DNA. No translation available.
BC001633 mRNA. Translation: AAH01633.2. Different initiation.
BC010041 mRNA. Translation: AAH10041.2. Different initiation.
Y11797 Genomic DNA. Translation: CAA72493.1.
PIRiS62767.
S68466.
RefSeqiNP_003312.3. NM_003321.4.
XP_016879108.1. XM_017023619.1.
UniGeneiHs.12084.

Genome annotation databases

EnsembliENST00000313511; ENSP00000322439; ENSG00000178952.
GeneIDi7284.
KEGGihsa:7284.
UCSCiuc002drh.2. human.

Similar proteinsi

Entry informationi

Entry nameiEFTU_HUMAN
AccessioniPrimary (citable) accession number: P49411
Secondary accession number(s): O15276
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: September 27, 2017
This is version 188 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families