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Protein

Plasma membrane ATPase

Gene

PMA1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Enzyme regulationi

Activated by high pH or also by potassium ions when the medium pH is low.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3594-aspartylphosphate intermediateBy similarity1
Metal bindingi615MagnesiumBy similarity1
Metal bindingi619MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processHydrogen ion transport, Ion transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane ATPase (EC:3.6.3.6)
Alternative name(s):
Proton pump
Gene namesi
Name:PMA1
Ordered Locus Names:KLLA0A09031g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 96CytoplasmicSequence analysisAdd BLAST96
Transmembranei97 – 117Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini118 – 121ExtracellularSequence analysis4
Transmembranei122 – 141Helical; Name=2Sequence analysisAdd BLAST20
Topological domaini142 – 272CytoplasmicSequence analysisAdd BLAST131
Transmembranei273 – 294Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini295 – 305ExtracellularSequence analysisAdd BLAST11
Transmembranei306 – 328Helical; Name=4Sequence analysisAdd BLAST23
Topological domaini329 – 700CytoplasmicSequence analysisAdd BLAST372
Transmembranei701 – 719Helical; Name=5Sequence analysisAdd BLAST19
Topological domaini720 – 735ExtracellularSequence analysisAdd BLAST16
Transmembranei736 – 755Helical; Name=6Sequence analysisAdd BLAST20
Topological domaini756 – 805CytoplasmicSequence analysisAdd BLAST50
Transmembranei806 – 826Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini827 – 838ExtracellularSequence analysisAdd BLAST12
Transmembranei839 – 855Helical; Name=8Sequence analysisAdd BLAST17
Topological domaini856 – 899CytoplasmicSequence analysisAdd BLAST44

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi669M → I in 3.3; low capacity to pump out protons. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462671 – 899Plasma membrane ATPaseAdd BLAST899

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP49380

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_451395.1

Structurei

3D structure databases

ProteinModelPortaliP49380
SMRiP49380
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi566 – 571Poly-Gly6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0205 Eukaryota
COG0474 LUCA
HOGENOMiHOG000160005
InParanoidiP49380
KOiK01535
OMAiHKYNVVE
OrthoDBiEOG092C0HLD

Family and domain databases

CDDicd02076 P-type_ATPase_H, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006534 P-type_ATPase_IIIA
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01647 ATPase-IIIA_H, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

P49380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAATEPTKE KPVNNQDSDD EDEDIDQLIE DLQSHHGLDD ESEDDEHVAA
60 70 80 90 100
GSARPVPEEL LQTDPSYGLT SDEVTKRRKK YGLNQMSEET ENLFVKFLMF
110 120 130 140 150
FIGPIQFVME AAAILAAGLE DWVDFGVICG LLFLNAAVGF IQEYQAGSIV
160 170 180 190 200
DELKKTLANS AVVIRDGNLV EVPSNEVVPG DILQLEDGVV IPADGRLVTE
210 220 230 240 250
DCFIQIDQSA ITGESLAVDK RFGDSTFSSS TVKRGEAFMI VTATGDSTFV
260 270 280 290 300
GRAAALVNKA AAGSGHFTEV LNGIGTILLI LVIVTLLLVW VASFYRTNKI
310 320 330 340 350
VRILRYTLAI TIVGVPVGLP AVVTTTMAVG AAYLAKKQAI VQKLSAIESL
360 370 380 390 400
AGVEILCSDK TGTLTKNKLS LHEPYTVEGV DPDDLMLTAC LAASRKKKGL
410 420 430 440 450
DAIDKAFLKS LISYPRAKAA LTKYKLLEFH PFDPVSKKVT AIVESPEGER
460 470 480 490 500
IICVKGAPLF VLKTVEEEHP IPEDVRENYE NKVAELASRG FRALGVARKR
510 520 530 540 550
GEGHWEILGV MPCMDPPRDD TAQTVNEARH LGLRVKMLTG DAVGIAKETC
560 570 580 590 600
RQLGLGTNIY NAERLGLGGG GDMPGSELAD FVENADGFAE VFPQHKYNVV
610 620 630 640 650
EILQQRGYLV AMTGDGVNDA PSLKKADTGI AVEGATDAAR SAADIVFLAP
660 670 680 690 700
GLSAIIDALK TSRQIFHRMY SYVVYRIALS LHLEIFLGLW IAILNRSLNI
710 720 730 740 750
DLVVFIAIFA DVATLAIAYD NAPYSPKPVK WNLRRLWGMS VILGIILAIG
760 770 780 790 800
TWITLTTMFV PKGGIIQNFG SIDGVLFLQI SLTENWLIFI TRAAGPFWSS
810 820 830 840 850
IPSWQLSGAV LIVDIIATMF CLFGWWSQNW NDIVTVVRVW IFSFGVFCVM
860 870 880 890
GGAYYMMSES EAFDRFMNGK SRRDKPSGRS VEDFLMAMQR VSTQHEKEN
Length:899
Mass (Da):98,260
Last modified:February 1, 1996 - v1
Checksum:iF29DC853BDCF4396
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37875 Genomic DNA Translation: AAA69688.1
CR382121 Genomic DNA Translation: CAH02983.1
RefSeqiXP_451395.1, XM_451395.1

Genome annotation databases

EnsemblFungiiCAH02983; CAH02983; KLLA0_A09031g
GeneIDi2896449
KEGGikla:KLLA0A09031g

Similar proteinsi

Entry informationi

Entry nameiPMA1_KLULA
AccessioniPrimary (citable) accession number: P49380
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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