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P49371 (HUGA_DOLMA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hyaluronidase

Short name=Hya
EC=3.2.1.35
Alternative name(s):
Allergen Dol m II
Hyaluronoglucosaminidase
Allergen=Dol m 2
OrganismDolichovespula maculata (Bald-faced hornet) (Vespula maculata)
Taxonomic identifier7441 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaeVespinaeDolichovespula

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides By similarity.

Catalytic activity

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Allergenic properties

Causes an allergic reaction in human.

Sequence similarities

Belongs to the glycosyl hydrolase 56 family.

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseAllergen
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

defense response

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhyalurononglucosaminidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331Hyaluronidase
PRO_0000191285

Sites

Active site1091Proton donor By similarity

Amino acid modifications

Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation3251N-linked (GlcNAc...) Potential
Disulfide bond19 ↔ 308 By similarity
Disulfide bond185 ↔ 197 By similarity

Sequences

Sequence LengthMass (Da)Tools
P49371 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: DD07C11CE7865570

FASTA33138,929
        10         20         30         40         50         60 
SERPKRVFNI YWNVPTFMCH QYGLYFDEVT NFNIKHNSKD DFQGDKISIF YDPGEFPALL 

        70         80         90        100        110        120 
PLKEGNYKIR NGGVPQEGNI TIHLQRFIEN LDKTYPNRNF NGIGVIDFER WRPIFRQNWG 

       130        140        150        160        170        180 
NMMIHKKFSI DLVRNEHPFW DKKMIELEAS KRFEKYARLF MEETLKLAKK TRKQADWGYY 

       190        200        210        220        230        240 
GYPYCFNMSP NNLVPDCDAT AMLENDKMSW LFNNQNVLLP SVYIRHELTP DQRVGLVQGR 

       250        260        270        280        290        300 
VKEAVRISNN LKHSPKVLSY WWYVYQDDTN TFLTETDVKK TFQEIAINGG DGIIIWGSSS 

       310        320        330 
DVNSLSKCKR LREYLLTVLG PITVNVTETV N 

« Hide

References

[1]"Sequence identity and antigenic cross-reactivity of white face hornet venom allergen, also a hyaluronidase, with other proteins."
Lu G., Kochoumian L., King T.P.
J. Biol. Chem. 270:4457-4465(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-45.
Tissue: Venom and Venom gland.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L34548 mRNA. Translation: AAA68279.1.
PIRA56090.

3D structure databases

ProteinModelPortalP49371.
SMRP49371. Positions 6-328.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome3273. Dol m 2.0101.
329. Dol m 2.
CAZyGH56. Glycoside Hydrolase Family 56.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view]
PANTHERPTHR11769. PTHR11769. 1 hit.
PfamPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PRINTSPR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHUGA_DOLMA
AccessionPrimary (citable) accession number: P49371
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: December 11, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Allergens

Nomenclature of allergens and list of entries