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Protein

Hyaluronidase

Gene
N/A
Organism
Dolichovespula maculata (Bald-faced hornet) (Vespula maculata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides.By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei109 – 1091Proton donorBy similarity

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase (EC:3.2.1.35)
Short name:
Hya
Alternative name(s):
Allergen Dol m II
Hyaluronoglucosaminidase
Allergen: Dol m 2
OrganismiDolichovespula maculata (Bald-faced hornet) (Vespula maculata)
Taxonomic identifieri7441 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaeVespinaeDolichovespula

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3273. Dol m 2.0101.
329. Dol m 2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331HyaluronidasePRO_0000191285Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi19 ↔ 308By similarity
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi185 ↔ 197By similarity
Glycosylationi325 – 3251N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

3D structure databases

ProteinModelPortaliP49371.
SMRiP49371. Positions 6-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PRINTSiPR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P49371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SERPKRVFNI YWNVPTFMCH QYGLYFDEVT NFNIKHNSKD DFQGDKISIF
60 70 80 90 100
YDPGEFPALL PLKEGNYKIR NGGVPQEGNI TIHLQRFIEN LDKTYPNRNF
110 120 130 140 150
NGIGVIDFER WRPIFRQNWG NMMIHKKFSI DLVRNEHPFW DKKMIELEAS
160 170 180 190 200
KRFEKYARLF MEETLKLAKK TRKQADWGYY GYPYCFNMSP NNLVPDCDAT
210 220 230 240 250
AMLENDKMSW LFNNQNVLLP SVYIRHELTP DQRVGLVQGR VKEAVRISNN
260 270 280 290 300
LKHSPKVLSY WWYVYQDDTN TFLTETDVKK TFQEIAINGG DGIIIWGSSS
310 320 330
DVNSLSKCKR LREYLLTVLG PITVNVTETV N
Length:331
Mass (Da):38,929
Last modified:February 1, 1996 - v1
Checksum:iDD07C11CE7865570
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34548 mRNA. Translation: AAA68279.1.
PIRiA56090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34548 mRNA. Translation: AAA68279.1.
PIRiA56090.

3D structure databases

ProteinModelPortaliP49371.
SMRiP49371. Positions 6-328.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3273. Dol m 2.0101.
329. Dol m 2.
CAZyiGH56. Glycoside Hydrolase Family 56.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PRINTSiPR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Sequence identity and antigenic cross-reactivity of white face hornet venom allergen, also a hyaluronidase, with other proteins."
    Lu G., Kochoumian L., King T.P.
    J. Biol. Chem. 270:4457-4465(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-45.
    Tissue: Venom and Venom gland.

Entry informationi

Entry nameiHUGA_DOLMA
AccessioniPrimary (citable) accession number: P49371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: January 7, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.