P49371 (HUGA_DOLMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Hyaluronidase Short name=Hya EC=3.2.1.35 Alternative name(s): Allergen Dol m II Hyaluronoglucosaminidase Allergen=Dol m 2
Organism	Dolichovespula maculata (Bald-faced hornet) (Vespula maculata)
Taxonomic identifier	7441 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Vespinae › Dolichovespula

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides By similarity.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Hyaluronidase		PRO_0000191285
Sites
	Active site	109	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	325	1	N-linked (GlcNAc...) Potential
	Disulfide bond	19 ↔ 308		By similarity
	Disulfide bond	185 ↔ 197		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49371 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: DD07C11CE7865570	FASTA	331	38,929
	10 20 30 40 50 60 SERPKRVFNI YWNVPTFMCH QYGLYFDEVT NFNIKHNSKD DFQGDKISIF YDPGEFPALL 70 80 90 100 110 120 PLKEGNYKIR NGGVPQEGNI TIHLQRFIEN LDKTYPNRNF NGIGVIDFER WRPIFRQNWG 130 140 150 160 170 180 NMMIHKKFSI DLVRNEHPFW DKKMIELEAS KRFEKYARLF MEETLKLAKK TRKQADWGYY 190 200 210 220 230 240 GYPYCFNMSP NNLVPDCDAT AMLENDKMSW LFNNQNVLLP SVYIRHELTP DQRVGLVQGR 250 260 270 280 290 300 VKEAVRISNN LKHSPKVLSY WWYVYQDDTN TFLTETDVKK TFQEIAINGG DGIIIWGSSS 310 320 330 DVNSLSKCKR LREYLLTVLG PITVNVTETV N

References

[1]

"Sequence identity and antigenic cross-reactivity of white face hornet venom allergen, also a hyaluronidase, with other proteins." Lu G., Kochoumian L., King T.P. J. Biol. Chem. 270:4457-4465(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-45. Tissue: Venom and Venom gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L34548 mRNA. Translation: AAA68279.1.
PIR	A56090.
3D structure databases
ProteinModelPortal	P49371.
SMR	P49371. Positions 6-328.
ModBase	Search...
Protein family/group databases
Allergome	3273. Dol m 2.0101. 329. Dol m 2.
CAZy	GH56. Glycoside Hydrolase Family 56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR001329. Glyco_hydro_56_Api/Dol. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
PANTHER	PTHR11769. PTHR11769. 1 hit. PTHR11769:SF2. PTHR11769:SF2. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PRINTS	PR00846. GLHYDRLASE56. PR00847. HYALURONDASE.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUGA_DOLMA

Accession

Primary (citable) accession number: P49371

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)