SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P49370

- HUGAA_VESVU

UniProt

P49370 - HUGAA_VESVU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Hyaluronidase A

Gene
N/A
Organism
Vespula vulgaris (Yellow jacket) (Wasp)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides By similarity.

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei109 – 1091Proton donor Inferred

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase A (EC:3.2.1.35)
Short name:
Hya A
Alternative name(s):
Allergen Ves v II
Hyaluronoglucosaminidase A
Allergen: Ves v 2a
OrganismiVespula vulgaris (Yellow jacket) (Wasp)
Taxonomic identifieri7454 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaeVespinaeVespula

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3521. Ves v 2.0101.
669. Ves v 2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Hyaluronidase APRO_0000191286Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi19 ↔ 3081 Publication
Glycosylationi79 – 791N-linked (GlcNAc...)1 Publication
Glycosylationi99 – 991N-linked (GlcNAc...)1 Publication
Glycosylationi127 – 1271N-linked (GlcNAc...)1 Publication
Disulfide bondi185 ↔ 1971 Publication
Glycosylationi325 – 3251N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
331
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 135
Helixi16 – 227
Helixi29 – 324
Helixi38 – 403
Beta strandi45 – 528
Beta strandi58 – 614
Beta strandi63 – 653
Beta strandi67 – 704
Helixi75 – 773
Helixi80 – 9415
Beta strandi102 – 1076
Helixi115 – 1173
Helixi120 – 1234
Helixi124 – 13613
Helixi142 – 17130
Beta strandi175 – 1806
Beta strandi194 – 1963
Helixi199 – 2068
Helixi209 – 2124
Beta strandi216 – 2194
Helixi230 – 25021
Beta strandi251 – 2533
Beta strandi256 – 26510
Beta strandi268 – 2725
Helixi275 – 28713
Beta strandi292 – 2965
Helixi299 – 3024
Helixi305 – 31713
Helixi319 – 32810

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ATMX-ray2.00A1-331[»]
ProteinModelPortaliP49370.
SMRiP49370. Positions 6-329.

Miscellaneous databases

EvolutionaryTraceiP49370.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PRINTSiPR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P49370-1 [UniParc]FASTAAdd to Basket

« Hide

SERPKRVFNI YWNVPTFMCH QYDLYFDEVT NFNIKRNSKD DFQGDKIAIF    50
YDPGEFPALL SLKDGKYKKR NGGVPQEGNI TIHLQKFIEN LDKIYPNRNF 100
SGIGVIDFER WRPIFRQNWG NMKIHKNFSI DLVRNEHPTW NKKMIELEAS 150
KRFEKYARFF MEETLKLAKK TRKQADWGYY GYPYCFNMSP NNLVPECDVT 200
AMHENDKMSW LFNNQNVLLP SVYVRQELTP DQRIGLVQGR VKEAVRISNN 250
LKHSPKVLSY WWYVYQDETN TFLTETDVKK TFQEIVINGG DGIIIWGSSS 300
DVNSLSKCKR LQDYLLTVLG PIAINVTEAV N 331
Length:331
Mass (Da):38,933
Last modified:February 1, 1996 - v1
Checksum:iF800F7C0F19D6AEC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11S → T AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43562 mRNA. Translation: AAB48073.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L43562 mRNA. Translation: AAB48073.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ATM X-ray 2.00 A 1-331 [» ]
ProteinModelPortali P49370.
SMRi P49370. Positions 6-329.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 3521. Ves v 2.0101.
669. Ves v 2.
CAZyi GH56. Glycoside Hydrolase Family 56.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P49370.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view ]
PANTHERi PTHR11769. PTHR11769. 1 hit.
Pfami PF01630. Glyco_hydro_56. 1 hit.
[Graphical view ]
PRINTSi PR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Yellow jacket venom allergens, hyaluronidase and phospholipase: sequence similarity and antigenic cross-reactivity with their hornet and wasp homologs and possible implications for clinical allergy."
    King T.P., Lu G., Gonzalez M., Qian N., Soldatova L.
    J. Allergy Clin. Immunol. 98:588-600(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-13; 205-224 AND 260-268.
    Tissue: Venom and Venom gland.
  2. "Dissecting cross-reactivity in hymenoptera venom allergy by circumvention of alpha-1,3-core fucosylation."
    Seismann H., Blank S., Braren I., Greunke K., Cifuentes L., Grunwald T., Bredehorst R., Ollert M., Spillner E.
    Mol. Immunol. 47:799-808(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.
  3. "Structure of recombinant Ves v 2 at 2.0 Angstrom resolution: structural analysis of an allergenic hyaluronidase from wasp venom."
    Skov L.K., Seppaelae U., Coen J.J.F., Crickmore N., King T.P., Monsalve R., Kastrup J.S., Spangfort M.D., Gajhede M.
    Acta Crystallogr. D 62:595-604(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-331, PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY, GLYCOSYLATION AT ASN-79; ASN-99 AND ASN-127, DISULFIDE BONDS.

Entry informationi

Entry nameiHUGAA_VESVU
AccessioniPrimary (citable) accession number: P49370
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi