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P49370

- HUGAA_VESVU

UniProt

P49370 - HUGAA_VESVU

Protein

Hyaluronidase A

Gene
N/A
Organism
Vespula vulgaris (Yellow jacket) (Wasp)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides.By similarity

    Catalytic activityi

    Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei109 – 1091Proton donorCurated

    GO - Molecular functioni

    1. hyalurononglucosaminidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. defense response Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH56. Glycoside Hydrolase Family 56.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronidase A (EC:3.2.1.35)
    Short name:
    Hya A
    Alternative name(s):
    Allergen Ves v II
    Hyaluronoglucosaminidase A
    Allergen: Ves v 2a
    OrganismiVespula vulgaris (Yellow jacket) (Wasp)
    Taxonomic identifieri7454 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaeVespinaeVespula

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human.

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei3521. Ves v 2.0101.
    669. Ves v 2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 331331Hyaluronidase APRO_0000191286Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi19 ↔ 3081 Publication
    Glycosylationi79 – 791N-linked (GlcNAc...)1 Publication
    Glycosylationi99 – 991N-linked (GlcNAc...)1 Publication
    Glycosylationi127 – 1271N-linked (GlcNAc...)1 Publication
    Disulfide bondi185 ↔ 1971 Publication
    Glycosylationi325 – 3251N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    Secondary structure

    1
    331
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi9 – 135
    Helixi16 – 227
    Helixi29 – 324
    Helixi38 – 403
    Beta strandi45 – 528
    Beta strandi58 – 614
    Beta strandi63 – 653
    Beta strandi67 – 704
    Helixi75 – 773
    Helixi80 – 9415
    Beta strandi102 – 1076
    Helixi115 – 1173
    Helixi120 – 1234
    Helixi124 – 13613
    Helixi142 – 17130
    Beta strandi175 – 1806
    Beta strandi194 – 1963
    Helixi199 – 2068
    Helixi209 – 2124
    Beta strandi216 – 2194
    Helixi230 – 25021
    Beta strandi251 – 2533
    Beta strandi256 – 26510
    Beta strandi268 – 2725
    Helixi275 – 28713
    Beta strandi292 – 2965
    Helixi299 – 3024
    Helixi305 – 31713
    Helixi319 – 32810

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ATMX-ray2.00A1-331[»]
    ProteinModelPortaliP49370.
    SMRiP49370. Positions 6-329.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP49370.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 56 family.Curated

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    IPR001329. Venom_Hyaluronidase.
    [Graphical view]
    PANTHERiPTHR11769. PTHR11769. 1 hit.
    PfamiPF01630. Glyco_hydro_56. 1 hit.
    [Graphical view]
    PRINTSiPR00846. GLHYDRLASE56.
    PR00847. HYALURONDASE.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P49370-1 [UniParc]FASTAAdd to Basket

    « Hide

    SERPKRVFNI YWNVPTFMCH QYDLYFDEVT NFNIKRNSKD DFQGDKIAIF    50
    YDPGEFPALL SLKDGKYKKR NGGVPQEGNI TIHLQKFIEN LDKIYPNRNF 100
    SGIGVIDFER WRPIFRQNWG NMKIHKNFSI DLVRNEHPTW NKKMIELEAS 150
    KRFEKYARFF MEETLKLAKK TRKQADWGYY GYPYCFNMSP NNLVPECDVT 200
    AMHENDKMSW LFNNQNVLLP SVYVRQELTP DQRIGLVQGR VKEAVRISNN 250
    LKHSPKVLSY WWYVYQDETN TFLTETDVKK TFQEIVINGG DGIIIWGSSS 300
    DVNSLSKCKR LQDYLLTVLG PIAINVTEAV N 331
    Length:331
    Mass (Da):38,933
    Last modified:February 1, 1996 - v1
    Checksum:iF800F7C0F19D6AEC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 11S → T AA sequence (PubMed:8828537)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43562 mRNA. Translation: AAB48073.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43562 mRNA. Translation: AAB48073.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ATM X-ray 2.00 A 1-331 [» ]
    ProteinModelPortali P49370.
    SMRi P49370. Positions 6-329.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 3521. Ves v 2.0101.
    669. Ves v 2.
    CAZyi GH56. Glycoside Hydrolase Family 56.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P49370.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    IPR001329. Venom_Hyaluronidase.
    [Graphical view ]
    PANTHERi PTHR11769. PTHR11769. 1 hit.
    Pfami PF01630. Glyco_hydro_56. 1 hit.
    [Graphical view ]
    PRINTSi PR00846. GLHYDRLASE56.
    PR00847. HYALURONDASE.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Yellow jacket venom allergens, hyaluronidase and phospholipase: sequence similarity and antigenic cross-reactivity with their hornet and wasp homologs and possible implications for clinical allergy."
      King T.P., Lu G., Gonzalez M., Qian N., Soldatova L.
      J. Allergy Clin. Immunol. 98:588-600(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-13; 205-224 AND 260-268.
      Tissue: Venom and Venom gland.
    2. "Dissecting cross-reactivity in hymenoptera venom allergy by circumvention of alpha-1,3-core fucosylation."
      Seismann H., Blank S., Braren I., Greunke K., Cifuentes L., Grunwald T., Bredehorst R., Ollert M., Spillner E.
      Mol. Immunol. 47:799-808(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY.
    3. "Structure of recombinant Ves v 2 at 2.0 Angstrom resolution: structural analysis of an allergenic hyaluronidase from wasp venom."
      Skov L.K., Seppaelae U., Coen J.J.F., Crickmore N., King T.P., Monsalve R., Kastrup J.S., Spangfort M.D., Gajhede M.
      Acta Crystallogr. D 62:595-604(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-331, PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY, GLYCOSYLATION AT ASN-79; ASN-99 AND ASN-127, DISULFIDE BONDS.

    Entry informationi

    Entry nameiHUGAA_VESVU
    AccessioniPrimary (citable) accession number: P49370
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3