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P49370 (HUGAA_VESVU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hyaluronidase A

Short name=Hya A
EC=3.2.1.35
Alternative name(s):
Allergen Ves v II
Hyaluronoglucosaminidase A
Allergen=Ves v 2a
OrganismVespula vulgaris (Yellow jacket) (Wasp)
Taxonomic identifier7454 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaeVespinaeVespula

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides By similarity.

Catalytic activity

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. Ref.2

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Allergenic properties

Causes an allergic reaction in human.

Sequence similarities

Belongs to the glycosyl hydrolase 56 family.

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseAllergen
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

defense response

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhyalurononglucosaminidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331Hyaluronidase A
PRO_0000191286

Sites

Active site1091Proton donor Probable

Amino acid modifications

Glycosylation791N-linked (GlcNAc...) Ref.3
Glycosylation991N-linked (GlcNAc...) Ref.3
Glycosylation1271N-linked (GlcNAc...) Ref.3
Glycosylation3251N-linked (GlcNAc...) Potential
Disulfide bond19 ↔ 308 Ref.3
Disulfide bond185 ↔ 197 Ref.3

Experimental info

Sequence conflict11S → T AA sequence Ref.1

Secondary structure

......................................................... 331
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P49370 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: F800F7C0F19D6AEC

FASTA33138,933
        10         20         30         40         50         60 
SERPKRVFNI YWNVPTFMCH QYDLYFDEVT NFNIKRNSKD DFQGDKIAIF YDPGEFPALL 

        70         80         90        100        110        120 
SLKDGKYKKR NGGVPQEGNI TIHLQKFIEN LDKIYPNRNF SGIGVIDFER WRPIFRQNWG 

       130        140        150        160        170        180 
NMKIHKNFSI DLVRNEHPTW NKKMIELEAS KRFEKYARFF MEETLKLAKK TRKQADWGYY 

       190        200        210        220        230        240 
GYPYCFNMSP NNLVPECDVT AMHENDKMSW LFNNQNVLLP SVYVRQELTP DQRIGLVQGR 

       250        260        270        280        290        300 
VKEAVRISNN LKHSPKVLSY WWYVYQDETN TFLTETDVKK TFQEIVINGG DGIIIWGSSS 

       310        320        330 
DVNSLSKCKR LQDYLLTVLG PIAINVTEAV N 

« Hide

References

[1]"Yellow jacket venom allergens, hyaluronidase and phospholipase: sequence similarity and antigenic cross-reactivity with their hornet and wasp homologs and possible implications for clinical allergy."
King T.P., Lu G., Gonzalez M., Qian N., Soldatova L.
J. Allergy Clin. Immunol. 98:588-600(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-13; 205-224 AND 260-268.
Tissue: Venom and Venom gland.
[2]"Dissecting cross-reactivity in hymenoptera venom allergy by circumvention of alpha-1,3-core fucosylation."
Seismann H., Blank S., Braren I., Greunke K., Cifuentes L., Grunwald T., Bredehorst R., Ollert M., Spillner E.
Mol. Immunol. 47:799-808(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY.
[3]"Structure of recombinant Ves v 2 at 2.0 Angstrom resolution: structural analysis of an allergenic hyaluronidase from wasp venom."
Skov L.K., Seppaelae U., Coen J.J.F., Crickmore N., King T.P., Monsalve R., Kastrup J.S., Spangfort M.D., Gajhede M.
Acta Crystallogr. D 62:595-604(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-331, PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY, GLYCOSYLATION AT ASN-79; ASN-99 AND ASN-127, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43562 mRNA. Translation: AAB48073.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2ATMX-ray2.00A1-331[»]
ProteinModelPortalP49370.
SMRP49370. Positions 6-329.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome3521. Ves v 2.0101.
669. Ves v 2.
CAZyGH56. Glycoside Hydrolase Family 56.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view]
PANTHERPTHR11769. PTHR11769. 1 hit.
PfamPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PRINTSPR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP49370.

Entry information

Entry nameHUGAA_VESVU
AccessionPrimary (citable) accession number: P49370
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Allergens

Nomenclature of allergens and list of entries