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P49370

- HUGAA_VESVU

UniProt

P49370 - HUGAA_VESVU

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Protein

Hyaluronidase A

Gene
N/A
Organism
Vespula vulgaris (Yellow jacket) (Wasp)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides.By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei109 – 1091Proton donorCurated

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase A (EC:3.2.1.35)
Short name:
Hya A
Alternative name(s):
Allergen Ves v II
Hyaluronoglucosaminidase A
Allergen: Ves v 2a
OrganismiVespula vulgaris (Yellow jacket) (Wasp)
Taxonomic identifieri7454 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaeVespinaeVespula

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3521. Ves v 2.0101.
669. Ves v 2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Hyaluronidase APRO_0000191286Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi19 ↔ 3081 Publication
Glycosylationi79 – 791N-linked (GlcNAc...)1 Publication
Glycosylationi99 – 991N-linked (GlcNAc...)1 Publication
Glycosylationi127 – 1271N-linked (GlcNAc...)1 Publication
Disulfide bondi185 ↔ 1971 Publication
Glycosylationi325 – 3251N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
331
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 135Combined sources
Helixi16 – 227Combined sources
Helixi29 – 324Combined sources
Helixi38 – 403Combined sources
Beta strandi45 – 528Combined sources
Beta strandi58 – 614Combined sources
Beta strandi63 – 653Combined sources
Beta strandi67 – 704Combined sources
Helixi75 – 773Combined sources
Helixi80 – 9415Combined sources
Beta strandi102 – 1076Combined sources
Helixi115 – 1173Combined sources
Helixi120 – 1234Combined sources
Helixi124 – 13613Combined sources
Helixi142 – 17130Combined sources
Beta strandi175 – 1806Combined sources
Beta strandi194 – 1963Combined sources
Helixi199 – 2068Combined sources
Helixi209 – 2124Combined sources
Beta strandi216 – 2194Combined sources
Helixi230 – 25021Combined sources
Beta strandi251 – 2533Combined sources
Beta strandi256 – 26510Combined sources
Beta strandi268 – 2725Combined sources
Helixi275 – 28713Combined sources
Beta strandi292 – 2965Combined sources
Helixi299 – 3024Combined sources
Helixi305 – 31713Combined sources
Helixi319 – 32810Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ATMX-ray2.00A1-331[»]
ProteinModelPortaliP49370.
SMRiP49370. Positions 6-329.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49370.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PRINTSiPR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P49370-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
SERPKRVFNI YWNVPTFMCH QYDLYFDEVT NFNIKRNSKD DFQGDKIAIF
60 70 80 90 100
YDPGEFPALL SLKDGKYKKR NGGVPQEGNI TIHLQKFIEN LDKIYPNRNF
110 120 130 140 150
SGIGVIDFER WRPIFRQNWG NMKIHKNFSI DLVRNEHPTW NKKMIELEAS
160 170 180 190 200
KRFEKYARFF MEETLKLAKK TRKQADWGYY GYPYCFNMSP NNLVPECDVT
210 220 230 240 250
AMHENDKMSW LFNNQNVLLP SVYVRQELTP DQRIGLVQGR VKEAVRISNN
260 270 280 290 300
LKHSPKVLSY WWYVYQDETN TFLTETDVKK TFQEIVINGG DGIIIWGSSS
310 320 330
DVNSLSKCKR LQDYLLTVLG PIAINVTEAV N
Length:331
Mass (Da):38,933
Last modified:February 1, 1996 - v1
Checksum:iF800F7C0F19D6AEC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11S → T AA sequence (PubMed:8828537)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43562 mRNA. Translation: AAB48073.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43562 mRNA. Translation: AAB48073.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ATM X-ray 2.00 A 1-331 [» ]
ProteinModelPortali P49370.
SMRi P49370. Positions 6-329.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 3521. Ves v 2.0101.
669. Ves v 2.
CAZyi GH56. Glycoside Hydrolase Family 56.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P49370.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view ]
PANTHERi PTHR11769. PTHR11769. 1 hit.
Pfami PF01630. Glyco_hydro_56. 1 hit.
[Graphical view ]
PRINTSi PR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Yellow jacket venom allergens, hyaluronidase and phospholipase: sequence similarity and antigenic cross-reactivity with their hornet and wasp homologs and possible implications for clinical allergy."
    King T.P., Lu G., Gonzalez M., Qian N., Soldatova L.
    J. Allergy Clin. Immunol. 98:588-600(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-13; 205-224 AND 260-268.
    Tissue: Venom and Venom gland.
  2. "Dissecting cross-reactivity in hymenoptera venom allergy by circumvention of alpha-1,3-core fucosylation."
    Seismann H., Blank S., Braren I., Greunke K., Cifuentes L., Grunwald T., Bredehorst R., Ollert M., Spillner E.
    Mol. Immunol. 47:799-808(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.
  3. "Structure of recombinant Ves v 2 at 2.0 Angstrom resolution: structural analysis of an allergenic hyaluronidase from wasp venom."
    Skov L.K., Seppaelae U., Coen J.J.F., Crickmore N., King T.P., Monsalve R., Kastrup J.S., Spangfort M.D., Gajhede M.
    Acta Crystallogr. D 62:595-604(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-331, PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY, GLYCOSYLATION AT ASN-79; ASN-99 AND ASN-127, DISULFIDE BONDS.

Entry informationi

Entry nameiHUGAA_VESVU
AccessioniPrimary (citable) accession number: P49370
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3