P49370 (HUGAA_VESVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Hyaluronidase A Short name=Hya A EC=3.2.1.35 Alternative name(s): Allergen Ves v II Hyaluronoglucosaminidase A Allergen=Ves v 2a
Organism	Vespula vulgaris (Yellow jacket) (Wasp)
Taxonomic identifier	7454 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Vespinae › Vespula

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in reproduction By similarity.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. Ref.2
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 331

331

Hyaluronidase A

PRO_0000191286

Sites

Active site

109

1

Proton donor Probable

Amino acid modifications

Glycosylation

79

1

N-linked (GlcNAc...) Ref.3

Glycosylation

99

1

N-linked (GlcNAc...) Ref.3

Glycosylation

127

1

N-linked (GlcNAc...) Ref.3

Glycosylation

325

1

N-linked (GlcNAc...) Potential

Disulfide bond

19 ↔ 308

Ref.3

Disulfide bond

185 ↔ 197

Ref.3

Experimental info

Sequence conflict

1

1

S → T AA sequence Ref.1

Secondary structure

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331

Helix Strand Turn

Details...

Beta strand

9 – 13

5

Helix

16 – 22

7

Helix

29 – 32

4

Helix

38 – 40

3

Beta strand

45 – 52

8

Beta strand

58 – 61

4

Beta strand

63 – 65

3

Beta strand

67 – 70

4

Helix

75 – 77

3

Helix

80 – 94

15

Beta strand

102 – 107

6

Helix

115 – 117

3

Helix

120 – 123

4

Helix

124 – 136

13

Helix

142 – 171

30

Beta strand

175 – 180

6

Beta strand

194 – 196

3

Helix

199 – 206

8

Helix

209 – 212

4

Beta strand

216 – 219

4

Helix

230 – 250

21

Beta strand

251 – 253

3

Beta strand

256 – 265

10

Beta strand

268 – 272

5

Helix

275 – 287

13

Beta strand

292 – 296

5

Helix

299 – 302

4

Helix

305 – 317

13

Helix

319 – 328

10

Sequences

	Sequence		Length	Mass (Da)
	P49370 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: F800F7C0F19D6AEC	FASTA	331	38,933
	10 20 30 40 50 60 SERPKRVFNI YWNVPTFMCH QYDLYFDEVT NFNIKRNSKD DFQGDKIAIF YDPGEFPALL 70 80 90 100 110 120 SLKDGKYKKR NGGVPQEGNI TIHLQKFIEN LDKIYPNRNF SGIGVIDFER WRPIFRQNWG 130 140 150 160 170 180 NMKIHKNFSI DLVRNEHPTW NKKMIELEAS KRFEKYARFF MEETLKLAKK TRKQADWGYY 190 200 210 220 230 240 GYPYCFNMSP NNLVPECDVT AMHENDKMSW LFNNQNVLLP SVYVRQELTP DQRIGLVQGR 250 260 270 280 290 300 VKEAVRISNN LKHSPKVLSY WWYVYQDETN TFLTETDVKK TFQEIVINGG DGIIIWGSSS 310 320 330 DVNSLSKCKR LQDYLLTVLG PIAINVTEAV N

References

[1]	"Yellow jacket venom allergens, hyaluronidase and phospholipase: sequence similarity and antigenic cross-reactivity with their hornet and wasp homologs and possible implications for clinical allergy." King T.P., Lu G., Gonzalez M., Qian N., Soldatova L. J. Allergy Clin. Immunol. 98:588-600(1996) [PubMed: 8828537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-13; 205-224 AND 260-268. Tissue: Venom and Venom gland.
[2]	"Dissecting cross-reactivity in hymenoptera venom allergy by circumvention of alpha-1,3-core fucosylation." Seismann H., Blank S., Braren I., Greunke K., Cifuentes L., Grunwald T., Bredehorst R., Ollert M., Spillner E. Mol. Immunol. 47:799-808(2010) [PubMed: 19896717] [Abstract] Cited for: CATALYTIC ACTIVITY.
[3]	"Structure of recombinant Ves v 2 at 2.0 Angstrom resolution: structural analysis of an allergenic hyaluronidase from wasp venom." Skov L.K., Seppaelae U., Coen J.J.F., Crickmore N., King T.P., Monsalve R., Kastrup J.S., Spangfort M.D., Gajhede M. Acta Crystallogr. D 62:595-604(2006) [PubMed: 16699186] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-331, PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY, GLYCOSYLATION AT ASN-79; ASN-99 AND ASN-127, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L43562 mRNA. Translation: AAB48073.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ATM	X-ray	2.00	A	1-331	[»]

ProteinModelPortal

P49370.

SMR

P49370. Positions 6-329.

ModBase

Search...

Protein family/group databases

Allergome

3521. Ves v 2.0101.
669. Ves v 2.

CAZy

GH56. Glycoside Hydrolase Family 56.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR001329. Glyco_hydro_56_Api/Dol.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

PANTHER

PTHR11769. Glyco_hydro_56. 1 hit.
PTHR11769:SF2. Glyco_hydro_56_Api/Dol. 1 hit.

Pfam

PF01630. Glyco_hydro_56. 1 hit.
[Graphical view]

PRINTS

PR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

ProtoNet

Search...

Entry information

Entry name

HUGAA_VESVU

Accession

Primary (citable) accession number: P49370

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 31, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)