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P49364

- GCST_PEA

UniProt

P49364 - GCST_PEA

Protein

Aminomethyltransferase, mitochondrial

Gene

GDCST

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    The glycine cleavage system catalyzes the degradation of glycine.

    Catalytic activityi

    [Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei235 – 2351SubstrateBy similarity
    Binding sitei266 – 2661SubstrateBy similarity
    Binding sitei404 – 4041SubstrateBy similarity

    GO - Molecular functioni

    1. aminomethyltransferase activity Source: UniProtKB-EC
    2. transaminase activity Source: UniProtKB-KW

    GO - Biological processi

    1. glycine catabolic process Source: InterPro

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminomethyltransferase, mitochondrial (EC:2.1.2.10)
    Alternative name(s):
    Glycine cleavage system T protein
    Short name:
    GCVT
    Gene namesi
    Name:GDCST
    Synonyms:GDCT
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    Subcellular locationi

    GO - Cellular componenti

    1. glycine cleavage complex Source: UniProtKB
    2. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3030MitochondrionAdd
    BLAST
    Chaini31 – 408378Aminomethyltransferase, mitochondrialPRO_0000010763Add
    BLAST

    Proteomic databases

    PRIDEiP49364.
    ProMEXiP49364.

    Interactioni

    Subunit structurei

    The glycine cleavage system is composed of four proteins: P, T, L and H.

    Protein-protein interaction databases

    IntActiP49364. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP49364.
    SMRiP49364. Positions 36-406.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GcvT family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di2.40.30.110. 1 hit.
    3.30.1360.120. 2 hits.
    InterProiIPR006223. GCS_T.
    IPR028896. GCST/DmdA.
    IPR013977. GCV_T_C.
    IPR006222. GCV_T_N.
    IPR029043. GcvT/YgfZ_C.
    IPR027266. TrmE/GcvT_dom1.
    [Graphical view]
    PANTHERiPTHR13847:SF5. PTHR13847:SF5. 1 hit.
    PfamiPF01571. GCV_T. 1 hit.
    PF08669. GCV_T_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006487. GcvT. 1 hit.
    SUPFAMiSSF101790. SSF101790. 1 hit.
    TIGRFAMsiTIGR00528. gcvT. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49364-1 [UniParc]FASTAAdd to Basket

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    MRGGLWQLGQ SITRRLANGG DKKAVARRCF ATESELKKTV LYDFHVAHGG    50
    KMVPFAGWSM PIQYKDSIMD STLNCRQNGS LFDVSHMCGL SLKGKDVVSF 100
    LEKLVIADVA ALAHGTGTLT VFTNEKGGAI DDSVITKVTD DHLYLVVNAG 150
    CRDKDLAHIE EHMKAFKAKG GDVSWHIHDE RSLLALQGPL AAPVLQHLTK 200
    EDLSKLYFGE FRVLDINGSQ CFLTRTGYTG EDGFEISVPS EHGVELAKAL 250
    LEKSEGKIRL TGLGARDSLR LEAGLCLYGN DLEQHITPIE AGLTWAIGKR 300
    RRAEGGFLGA DVILKQLADG PSIRRVGFIS SGPPPRSHSE IQDEGGNNIG 350
    EVTSGGFSPC LKKNIAIGYV KSGLHKAGTK VKIIIRGKQN EGVVTKMPFV 400
    PTKYYKPS 408
    Length:408
    Mass (Da):44,285
    Last modified:December 15, 1998 - v2
    Checksum:i41BB4724AC910793
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti98 – 981V → I in CAA52800. (PubMed:8223576)Curated
    Sequence conflicti114 – 1141H → N in CAA52800. (PubMed:8223576)Curated
    Sequence conflicti140 – 1401D → E in CAA52800. (PubMed:8223576)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74793 mRNA. Translation: CAA52800.1.
    Z25861 mRNA. Translation: CAA81080.1.
    AJ222771 Genomic DNA. Translation: CAA10976.1.
    PIRiS38370.
    S56661.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74793 mRNA. Translation: CAA52800.1 .
    Z25861 mRNA. Translation: CAA81080.1 .
    AJ222771 Genomic DNA. Translation: CAA10976.1 .
    PIRi S38370.
    S56661.

    3D structure databases

    ProteinModelPortali P49364.
    SMRi P49364. Positions 36-406.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P49364. 1 interaction.

    Proteomic databases

    PRIDEi P49364.
    ProMEXi P49364.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.40.30.110. 1 hit.
    3.30.1360.120. 2 hits.
    InterProi IPR006223. GCS_T.
    IPR028896. GCST/DmdA.
    IPR013977. GCV_T_C.
    IPR006222. GCV_T_N.
    IPR029043. GcvT/YgfZ_C.
    IPR027266. TrmE/GcvT_dom1.
    [Graphical view ]
    PANTHERi PTHR13847:SF5. PTHR13847:SF5. 1 hit.
    Pfami PF01571. GCV_T. 1 hit.
    PF08669. GCV_T_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006487. GcvT. 1 hit.
    SUPFAMi SSF101790. SSF101790. 1 hit.
    TIGRFAMsi TIGR00528. gcvT. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Glycine decarboxylase complex from higher plants. Molecular cloning, tissue distribution and mass spectrometry analyses of the T protein."
      Bourguignon J., Vauclare P., Merand V., Forest E., Neuburger M., Douce R.
      Eur. J. Biochem. 217:377-386(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Leaf.
    2. "T-protein of the glycine decarboxylase multienzyme complex: evidence for partial similarity to formyltetrahydrofolate synthetase."
      Kopriva S., Turner S.R., Rawsthorne S., Bauwe H.
      Plant Mol. Biol. 27:1215-1220(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Leaf.
    3. "The gene encoding T protein of the glycine decarboxylase complex involved in the mitochondrial step of the photorespiratory pathway in plants exhibits features of light-induced genes."
      Vauclare P., Macherel D., Douce R., Bourguignon J.
      Plant Mol. Biol. 37:309-318(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiGCST_PEA
    AccessioniPrimary (citable) accession number: P49364
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3