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P49364

- GCST_PEA

UniProt

P49364 - GCST_PEA

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Protein

Aminomethyltransferase, mitochondrial

Gene

GDCST

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine.

Catalytic activityi

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei235 – 2351SubstrateBy similarity
Binding sitei266 – 2661SubstrateBy similarity
Binding sitei404 – 4041SubstrateBy similarity

GO - Molecular functioni

  1. aminomethyltransferase activity Source: UniProtKB-EC
  2. transaminase activity Source: UniProtKB-KW

GO - Biological processi

  1. glycine catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Aminomethyltransferase, mitochondrial (EC:2.1.2.10)
Alternative name(s):
Glycine cleavage system T protein
Short name:
GCVT
Gene namesi
Name:GDCST
Synonyms:GDCT
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

  1. glycine cleavage complex Source: UniProtKB
  2. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030MitochondrionAdd
BLAST
Chaini31 – 408378Aminomethyltransferase, mitochondrialPRO_0000010763Add
BLAST

Proteomic databases

PRIDEiP49364.
ProMEXiP49364.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.

Protein-protein interaction databases

IntActiP49364. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP49364.
SMRiP49364. Positions 36-406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProiIPR006223. GCS_T.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PANTHERiPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49364-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRGGLWQLGQ SITRRLANGG DKKAVARRCF ATESELKKTV LYDFHVAHGG
60 70 80 90 100
KMVPFAGWSM PIQYKDSIMD STLNCRQNGS LFDVSHMCGL SLKGKDVVSF
110 120 130 140 150
LEKLVIADVA ALAHGTGTLT VFTNEKGGAI DDSVITKVTD DHLYLVVNAG
160 170 180 190 200
CRDKDLAHIE EHMKAFKAKG GDVSWHIHDE RSLLALQGPL AAPVLQHLTK
210 220 230 240 250
EDLSKLYFGE FRVLDINGSQ CFLTRTGYTG EDGFEISVPS EHGVELAKAL
260 270 280 290 300
LEKSEGKIRL TGLGARDSLR LEAGLCLYGN DLEQHITPIE AGLTWAIGKR
310 320 330 340 350
RRAEGGFLGA DVILKQLADG PSIRRVGFIS SGPPPRSHSE IQDEGGNNIG
360 370 380 390 400
EVTSGGFSPC LKKNIAIGYV KSGLHKAGTK VKIIIRGKQN EGVVTKMPFV

PTKYYKPS
Length:408
Mass (Da):44,285
Last modified:December 15, 1998 - v2
Checksum:i41BB4724AC910793
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti98 – 981V → I in CAA52800. (PubMed:8223576)Curated
Sequence conflicti114 – 1141H → N in CAA52800. (PubMed:8223576)Curated
Sequence conflicti140 – 1401D → E in CAA52800. (PubMed:8223576)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74793 mRNA. Translation: CAA52800.1.
Z25861 mRNA. Translation: CAA81080.1.
AJ222771 Genomic DNA. Translation: CAA10976.1.
PIRiS38370.
S56661.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74793 mRNA. Translation: CAA52800.1 .
Z25861 mRNA. Translation: CAA81080.1 .
AJ222771 Genomic DNA. Translation: CAA10976.1 .
PIRi S38370.
S56661.

3D structure databases

ProteinModelPortali P49364.
SMRi P49364. Positions 36-406.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P49364. 1 interaction.

Proteomic databases

PRIDEi P49364.
ProMEXi P49364.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProi IPR006223. GCS_T.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view ]
PANTHERi PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfami PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF006487. GcvT. 1 hit.
SUPFAMi SSF101790. SSF101790. 1 hit.
TIGRFAMsi TIGR00528. gcvT. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Glycine decarboxylase complex from higher plants. Molecular cloning, tissue distribution and mass spectrometry analyses of the T protein."
    Bourguignon J., Vauclare P., Merand V., Forest E., Neuburger M., Douce R.
    Eur. J. Biochem. 217:377-386(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Leaf.
  2. "T-protein of the glycine decarboxylase multienzyme complex: evidence for partial similarity to formyltetrahydrofolate synthetase."
    Kopriva S., Turner S.R., Rawsthorne S., Bauwe H.
    Plant Mol. Biol. 27:1215-1220(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.
  3. "The gene encoding T protein of the glycine decarboxylase complex involved in the mitochondrial step of the photorespiratory pathway in plants exhibits features of light-induced genes."
    Vauclare P., Macherel D., Douce R., Bourguignon J.
    Plant Mol. Biol. 37:309-318(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGCST_PEA
AccessioniPrimary (citable) accession number: P49364
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 15, 1998
Last modified: October 29, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3