P49364 (GCST_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase, mitochondrial
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein
Short name=GCVT

Gene names

Name:	GDCST
Synonyms:	GDCT

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine.
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Aminotransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	glycine cleavage complex Inferred from direct assay PubMed 3143355. Source: UniProtKB mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 30	30	Mitochondrion
	Chain	31 – 408	378	Aminomethyltransferase, mitochondrial		PRO_0000010763
Sites
	Binding site	235	1	Substrate By similarity
	Binding site	266	1	Substrate By similarity
	Binding site	404	1	Substrate By similarity
Experimental info
	Sequence conflict	98	1	V → I in CAA52800. Ref.1
	Sequence conflict	114	1	H → N in CAA52800. Ref.1
	Sequence conflict	140	1	D → E in CAA52800. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49364 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 41BB4724AC910793	FASTA	408	44,285
	10 20 30 40 50 60 MRGGLWQLGQ SITRRLANGG DKKAVARRCF ATESELKKTV LYDFHVAHGG KMVPFAGWSM 70 80 90 100 110 120 PIQYKDSIMD STLNCRQNGS LFDVSHMCGL SLKGKDVVSF LEKLVIADVA ALAHGTGTLT 130 140 150 160 170 180 VFTNEKGGAI DDSVITKVTD DHLYLVVNAG CRDKDLAHIE EHMKAFKAKG GDVSWHIHDE 190 200 210 220 230 240 RSLLALQGPL AAPVLQHLTK EDLSKLYFGE FRVLDINGSQ CFLTRTGYTG EDGFEISVPS 250 260 270 280 290 300 EHGVELAKAL LEKSEGKIRL TGLGARDSLR LEAGLCLYGN DLEQHITPIE AGLTWAIGKR 310 320 330 340 350 360 RRAEGGFLGA DVILKQLADG PSIRRVGFIS SGPPPRSHSE IQDEGGNNIG EVTSGGFSPC 370 380 390 400 LKKNIAIGYV KSGLHKAGTK VKIIIRGKQN EGVVTKMPFV PTKYYKPS

References

[1]	"Glycine decarboxylase complex from higher plants. Molecular cloning, tissue distribution and mass spectrometry analyses of the T protein." Bourguignon J., Vauclare P., Merand V., Forest E., Neuburger M., Douce R. Eur. J. Biochem. 217:377-386(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Leaf.
[2]	"T-protein of the glycine decarboxylase multienzyme complex: evidence for partial similarity to formyltetrahydrofolate synthetase." Kopriva S., Turner S.R., Rawsthorne S., Bauwe H. Plant Mol. Biol. 27:1215-1220(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[3]	"The gene encoding T protein of the glycine decarboxylase complex involved in the mitochondrial step of the photorespiratory pathway in plants exhibits features of light-induced genes." Vauclare P., Macherel D., Douce R., Bourguignon J. Plant Mol. Biol. 37:309-318(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74793 mRNA. Translation: CAA52800.1. Z25861 mRNA. Translation: CAA81080.1. AJ222771 Genomic DNA. Translation: CAA10976.1.
PIR	S38370. S56661.
3D structure databases
ProteinModelPortal	P49364.
SMR	P49364. Positions 36-406.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49364. 1 interaction.
Proteomic databases
PRIDE	P49364.
ProMEX	P49364.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.1360.120. 2 hits.
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR027266. TrmE/GcvT_dom1. [Graphical view]
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_PEA

Accession

Primary (citable) accession number: P49364

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program