Reviewed, UniProtKB/Swiss-Prot P49347 (CONB_CANEN)

Names and origin

Protein names	Recommended name:     Concanavalin B       Short name=Con B
Organism	Canavalia ensiformis (Jack bean) (Horse bean)
Taxonomic identifier	3823 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Canavalia

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May act as a carbohydrate-binding protein.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family.

Ontologies

Keywords
Domain	Signal
PTM	Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Chain

26 – 324

299

Concanavalin B

PRO_0000011989

Amino acid modifications

Glycosylation

309

1

N-linked (GlcNAc...) Potential

Secondary structure

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324

Helix Strand Turn

Details...

Helix

27 – 29

3

Beta strand

31 – 36

6

Helix

39 – 41

3

Helix

44 – 49

6

Beta strand

54 – 61

8

Turn

76 – 78

3

Turn

81 – 84

4

Helix

87 – 90

4

Helix

91 – 99

9

Beta strand

103 – 109

7

Beta strand

111 – 115

5

Helix

120 – 134

15

Beta strand

135 – 139

5

Beta strand

149 – 154

6

Helix

164 – 178

15

Beta strand

183 – 186

4

Beta strand

189 – 193

5

Turn

195 – 197

3

Helix

198 – 201

4

Turn

202 – 204

3

Beta strand

207 – 212

6

Turn

217 – 219

3

Helix

227 – 239

13

Beta strand

240 – 242

3

Beta strand

247 – 254

8

Helix

255 – 257

3

Helix

266 – 272

7

Helix

274 – 276

3

Helix

280 – 283

4

Beta strand

284 – 290

7

Helix

292 – 298

7

Helix

300 – 307

8

Sequences

	Sequence		Length	Mass (Da)
	P49347-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 539CA5D4B68397FE	FASTA	324	36,728
	10 20 30 40 50 60 MGCERKALIL MVVIWIMSFW TLSLADISST EIAVYWGQRE DGLLRDTCKT NNYKIVFISF 70 80 90 100 110 120 LDKFGCEIRK PELELEGVCG PSVGNPCSFL ESQIKECQRM GVKVFLALGG PKGTYSACSA 130 140 150 160 170 180 DYAKDLAEYL HTYFLSERRE GPLGKVALDG IHFDIQKPVD ELNWDNLLEE LYQIKDVYQS 190 200 210 220 230 240 TFLLSAAPGC LSPDEYLDNA IQTRHFDYIF VRFYNDRSCQ YSTGNIQRIR NAWLSWTKSV 250 260 270 280 290 300 YPRDKNLFLE LPASQATAPG GGYIPPSALI GQVLPYLPDL QTRYAGIALW NRQADKETGY 310 320 STNIIRYLNA TAMPFTSNLL KYPS

References

[1]	Schlesier B., Nong V., Horstmann C., Hennig M. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Cotyledon.
[2]	"Crystal structure of concanavalin B at 1.65-A resolution. An 'inactivated' chitinase from seeds of Canavalia ensiformis." Hennig M., Jansonius J.N., van Scheltinga A.C.T., Dijkstra B.W., Schlesier B. J. Mol. Biol. 254:237-246(1995) [PubMed: 7490746] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

X83426 mRNA. Translation: CAA58450.1.

PIR

S57649.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CNV	X-ray	1.65	A	26-324	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH18. Glycoside Hydrolase Family 18.

Family and domain databases

InterPro

IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

Pfam

PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]

PROSITE

PS01095. CHITINASE_18. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CONB_CANEN

Accession

Primary (citable) accession number: P49347

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)