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Protein

Calpastatin

Gene

CAST

Organism
Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Calpastatin
Alternative name(s):
Calpain inhibitor
Gene namesi
Name:CAST
OrganismiChlorocebus aethiops (Green monkey) (Cercopithecus aethiops)
Taxonomic identifieri9534 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeChlorocebus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›283›283CalpastatinPRO_0000147631Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki32 – 32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei50 – 501N6-acetyllysineBy similarity
Modified residuei87 – 871PhosphoserineBy similarity
Modified residuei133 – 1331PhosphoserineBy similarity
Modified residuei135 – 1351PhosphothreonineBy similarity
Modified residuei222 – 2221PhosphoserineBy similarity
Modified residuei243 – 2431PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP49342.

Interactioni

Protein-protein interaction databases

IntActiP49342. 1 interaction.

Structurei

Miscellaneous databases

EvolutionaryTraceiP49342.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati170 – 22253Inhibitory domain 1Add
BLAST

Domaini

Each of the four flexible inhibitory domains can inhibit one calcium-bound calpain molecule by occupying both sides of the active site.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG106383.

Family and domain databases

InterProiIPR026998. Calpastatin.
IPR001259. Prot_inh_calpain.
[Graphical view]
PANTHERiPTHR10077. PTHR10077. 1 hit.
PfamiPF00748. Calpain_inhib. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P49342-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPTETKAIP VSQQMEGPHL PNKKKHKKQA VKTEPEKKSQ STKLSVVHEK
60 70 80 90 100
KSQEGKPKEH TEQKSLPKPA SDTGSKDAHN KKAVSRSAEQ QPSEKSTEPK
110 120 130 140 150
TEPQDMVSAG GESVAGVAAT SGKPGDKKKE KKSLTPAVPV ESKPDKPSGK
160 170 180 190 200
SGMDAALDDL IDTLGGPEEI EEENTTYTGP EVSDPMSSTY IEELGKREVT
210 220 230 240 250
IPPKYRELLA KNEGITGPPA DSSKPVGPDD AIDALSSDFT CGSPTAAGKK
260 270 280
TEKEESTEVL KAQSAGTVRS AAPPQEKKRK VEK
Length:283
Mass (Da):30,170
Last modified:February 1, 1996 - v1
Checksum:i02743390B5D6F0F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei283 – 2831

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86248 mRNA. Translation: AAA52753.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86248 mRNA. Translation: AAA52753.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP49342. 1 interaction.

Proteomic databases

PRIDEiP49342.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG106383.

Miscellaneous databases

EvolutionaryTraceiP49342.

Family and domain databases

InterProiIPR026998. Calpastatin.
IPR001259. Prot_inh_calpain.
[Graphical view]
PANTHERiPTHR10077. PTHR10077. 1 hit.
PfamiPF00748. Calpain_inhib. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICAL_CHLAE
AccessioniPrimary (citable) accession number: P49342
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 14, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.