P49337 (WNT4_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-4

Gene names

Name:	WNT4
Synonyms:	WNT-4

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. May be an intracellular signaling molecule involved in segmentation of the forebrain into the neuromere D2 and in differentiation of the dorsal region of the spinal cord. Is likely to signal over only few cell diameters.
Subunit structure	Interacts with CPZ. Ref.3
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Predominantly expressed in the diencephalon neuromere D2.
Post-translational modification	Palmitoylation at Ser-212 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-78. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adrenal gland development Inferred from electronic annotation. Source: Compara androgen biosynthetic process Inferred from electronic annotation. Source: Compara anterior/posterior pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome branching involved in ureteric bud morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome cellular response to transforming growth factor beta stimulus Inferred from electronic annotation. Source: Compara embryonic epithelial tube formation Inferred from electronic annotation. Source: Compara endoderm development Inferred from Biological aspect of Ancestor. Source: RefGenome epithelial to mesenchymal transition Inferred from electronic annotation. Source: Compara establishment of protein localization to plasma membrane Inferred from electronic annotation. Source: Compara female gonad development Inferred from Biological aspect of Ancestor. Source: RefGenome female sex determination Inferred from electronic annotation. Source: Compara immature T cell proliferation in thymus Inferred from electronic annotation. Source: Compara liver development Inferred from electronic annotation. Source: Compara male gonad development Inferred from Biological aspect of Ancestor. Source: RefGenome mesenchymal to epithelial transition Inferred from electronic annotation. Source: Compara mesonephric tubule development Inferred from Biological aspect of Ancestor. Source: RefGenome metanephric nephron morphogenesis Inferred from electronic annotation. Source: Compara metanephros development Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of fibroblast growth factor receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of male gonad development Inferred from electronic annotation. Source: Compara negative regulation of testicular blood vessel morphogenesis Inferred from electronic annotation. Source: Compara negative regulation of testosterone biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara non-canonical Wnt receptor signaling pathway via MAPK cascade Inferred from electronic annotation. Source: Compara oocyte development Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of aldosterone biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of bone mineralization Inferred from electronic annotation. Source: Compara positive regulation of canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of collagen biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of cortisol biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of dermatome development Inferred from electronic annotation. Source: Compara positive regulation of meiosis Inferred from electronic annotation. Source: Compara positive regulation of osteoblast differentiation Inferred from electronic annotation. Source: Compara protein palmitoylation Inferred from electronic annotation. Source: Compara regulation of cell-cell adhesion Inferred from electronic annotation. Source: Compara renal vesicle formation Inferred from Biological aspect of Ancestor. Source: RefGenome renal vesicle induction Inferred from electronic annotation. Source: Compara smooth muscle cell differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome somatotropin secreting cell differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome tertiary branching involved in mammary gland duct morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome thyroid-stimulating hormone-secreting cell differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	Golgi apparatus Inferred from Biological aspect of Ancestor. Source: RefGenome cell surface Inferred from electronic annotation. Source: Compara extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome transcription corepressor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 351	329	Protein Wnt-4		PRO_0000041425
Amino acid modifications
	Lipidation	78	1	S-palmitoyl cysteine By similarity
	Lipidation	212	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	21	1	N-linked (GlcNAc...) Potential
	Glycosylation	88	1	N-linked (GlcNAc...) Potential
	Glycosylation	297	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P49337 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: D22DC689284A961C	FASTA	351	38,963
	10 20 30 40 50 60 MSPEYFLRSL LLIILATFSA NASNWLYLAK LSSVGSISEE ETCEKLKGLI QRQVQMCKRN 70 80 90 100 110 120 LEVMDSVRRG AQLAIEECQY QFRNRRWNCS TLDTLPVFGK VVTQGTREAA FVYAISSAGV 130 140 150 160 170 180 AFAVTRACSS GELDKCGCDR TVQGGSPQGF QWSGCSDNIA YGVAFSQSFV DVRERSKGAS 190 200 210 220 230 240 SNRALMNLHN NEAGRKAILN NMRVECKCHG VSGSCEFKTC WKAMPPFRKV GNVLKEKFDG 250 260 270 280 290 300 ATEVEQSEIG STKVLVPKNS QFKPHTDEDL VYLDSSPDFC DHDLKNGVLG TSGRQCNKTS 310 320 330 340 350 KAIDGCELMC CGRGFHTDEV EVVERCSCKF HWCCSVKCKP CHRVVEIHTC R

References

[1]	"Regional expression of the Cwnt-4 gene in developing chick central nervous system in relationship to the diencephalic neuromere D2 and a dorsal domain of the spinal cord." Yoshioka H., Ohuchi H., Nohno T., Fujiwara A., Tanda N., Kawakami Y., Noji S. Biochem. Biophys. Res. Commun. 203:1581-1588(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"Cloning and characterization of Wnt-4 and Wnt-11 cDNAs from chick embryo." Tanda N., Kawakami Y., Saito T., Noji S., Nohno T. DNA Seq. 5:277-281(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Carboxypeptidase Z (CPZ) modulates Wnt signaling and regulates the development of skeletal elements in the chicken." Moeller C., Swindell E.C., Kispert A., Eichele G. Development 130:5103-5111(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CPZ.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D31900 mRNA. Translation: BAA06698.1.
IPI	IPI00595359.
PIR	JC2451.
RefSeq	NP_990114.1. NM_204783.1.
UniGene	Gga.305.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000007633.
Proteomic databases
PaxDb	P49337.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000007645; ENSGALP00000007633; ENSGALG00000004790.
GeneID	395561.
KEGG	gga:395561.
Organism-specific databases
CTD	54361.
Phylogenomic databases
eggNOG	NOG284879.
GeneTree	ENSGT00690000101771.
HOGENOM	HOG000039529.
HOVERGEN	HBG001595.
InParanoid	P49337.
KO	K00408.
OMA	QVQICKR.
OrthoDB	EOG49P9ZP.
Gene expression databases
ArrayExpress	P49337.
Family and domain databases
InterPro	IPR005817. Wnt. IPR009142. Wnt4. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF19. PTHR12027:SF19. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01844. WNT4PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20815636.

Entry information

Entry name

WNT4_CHICK

Accession

Primary (citable) accession number: P49337

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program