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P49334

- TOM22_YEAST

UniProt

P49334 - TOM22_YEAST

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Protein

Mitochondrial import receptor subunit TOM22

Gene

TOM22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM70 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Docks TOM20 and TOM70 for interaction with the general TOM40 import pore (GIP) complex. May regulate the TOM machinery organization, stability and channel gating.3 Publications

GO - Biological processi

  1. protein import into mitochondrial matrix Source: SGD
  2. protein import into mitochondrial outer membrane Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33151-MONOMER.
ReactomeiREACT_189012. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM22
Alternative name(s):
Mitochondrial 17 kDa assembly protein
Mitochondrial 22 kDa outer membrane protein
Protein MAS17
Translocase of outer membrane 22 kDa subunit
Gene namesi
Name:TOM22
Synonyms:MAS17, MAS22, MOM22
Ordered Locus Names:YNL131W
ORF Names:N1217, N1862
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

SGDiS000005075. TOM22.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 9796CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei98 – 11922HelicalSequence AnalysisAdd
BLAST
Topological domaini120 – 15233Mitochondrial intermembraneSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of mitochondrial outer membrane Source: SGD
  2. mitochondrial outer membrane Source: Reactome
  3. mitochondrial outer membrane translocase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 152151Mitochondrial import receptor subunit TOM22PRO_0000076113Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441Phosphoserine2 Publications
Modified residuei46 – 461Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49334.
PaxDbiP49334.
PeptideAtlasiP49334.

Expressioni

Gene expression databases

GenevestigatoriP49334.

Interactioni

Subunit structurei

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70). Interacts with TOM20 and TOM70. Interacts with FCJ1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BAXQ078123EBI-12527,EBI-516580From a different organism.
SAM50P539696EBI-12527,EBI-28646
TIM21P532202EBI-12527,EBI-23128
TOM20P351804EBI-12527,EBI-12522
TOM40P236448EBI-12527,EBI-12539

Protein-protein interaction databases

BioGridi35696. 64 interactions.
DIPiDIP-2302N.
IntActiP49334. 17 interactions.
MINTiMINT-478366.
STRINGi4932.YNL131W.

Structurei

3D structure databases

ProteinModelPortaliP49334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Its cytoplasmic domain associates with the cytoplasmic domains of TOM20 and TOM70. Its intermembrane space domain provides a trans binding site for presequences and the single membrane anchor is required for a stable interaction between the GIP complex proteins.

Sequence similaritiesi

Belongs to the Tom22 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG321322.
HOGENOMiHOG000206849.
InParanoidiP49334.
KOiK17769.
OMAiLTQIDDE.
OrthoDBiEOG7D8694.

Family and domain databases

InterProiIPR005683. Tom22.
IPR020951. Tom22_fun.
[Graphical view]
PfamiPF04281. Tom22. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00986. 3a0801s05tom22. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49334-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVELTEIKDD VVQLDEPQFS RNQAIVEEKA SATNNDVVDD EDDSDSDFED
60 70 80 90 100
EFDENETLLD RIVALKDIVP PGKRQTISNF FGFTSSFVRN AFTKSGNLAW
110 120 130 140 150
TLTTTALLLG VPLSLSILAE QQLIEMEKTF DLQSDANNIL AQGEKDAAAT

AN
Length:152
Mass (Da):16,790
Last modified:January 23, 2007 - v3
Checksum:i6ACEC5D37D143CD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46843 Genomic DNA. Translation: CAA86894.1.
X82405 Genomic DNA. Translation: CAA57799.1.
X80348 Genomic DNA. Translation: CAA56588.1. Sequence problems.
Z71407 Genomic DNA. Translation: CAA96013.1.
BK006947 Genomic DNA. Translation: DAA10417.1.
PIRiS50250.
RefSeqiNP_014268.1. NM_001182969.1.

Genome annotation databases

EnsemblFungiiYNL131W; YNL131W; YNL131W.
GeneIDi855592.
KEGGisce:YNL131W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46843 Genomic DNA. Translation: CAA86894.1 .
X82405 Genomic DNA. Translation: CAA57799.1 .
X80348 Genomic DNA. Translation: CAA56588.1 . Sequence problems.
Z71407 Genomic DNA. Translation: CAA96013.1 .
BK006947 Genomic DNA. Translation: DAA10417.1 .
PIRi S50250.
RefSeqi NP_014268.1. NM_001182969.1.

3D structure databases

ProteinModelPortali P49334.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35696. 64 interactions.
DIPi DIP-2302N.
IntActi P49334. 17 interactions.
MINTi MINT-478366.
STRINGi 4932.YNL131W.

Protein family/group databases

TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBi P49334.
PaxDbi P49334.
PeptideAtlasi P49334.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL131W ; YNL131W ; YNL131W .
GeneIDi 855592.
KEGGi sce:YNL131W.

Organism-specific databases

SGDi S000005075. TOM22.

Phylogenomic databases

eggNOGi NOG321322.
HOGENOMi HOG000206849.
InParanoidi P49334.
KOi K17769.
OMAi LTQIDDE.
OrthoDBi EOG7D8694.

Enzyme and pathway databases

BioCyci YEAST:G3O-33151-MONOMER.
Reactomei REACT_189012. Mitochondrial protein import.

Miscellaneous databases

NextBioi 979738.

Gene expression databases

Genevestigatori P49334.

Family and domain databases

InterProi IPR005683. Tom22.
IPR020951. Tom22_fun.
[Graphical view ]
Pfami PF04281. Tom22. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00986. 3a0801s05tom22. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The mitochondrial receptor complex: Mom22 is essential for cell viability and directly interacts with preproteins."
    Hoenlinger A., Kuebrich M., Moczko M., Gaertner F., Mallet L., Bussereau F., Eckerskorn C., Lottspeich F., Dietmeier K., Jacquet M., Pfanner N.
    Mol. Cell. Biol. 15:3382-3389(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-17, FUNCTION.
    Strain: ATCC 204508 / S288c.
  2. "Identification of yeast MAS17 encoding the functional counterpart of the mitochondrial receptor complex protein MOM22 of Neurospora crassa."
    Nakai M., Endo T.
    FEBS Lett. 357:202-206(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SP1.
  3. "The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast."
    Lithgow T., Junne T., Suda K., Gratzer S., Schatz G.
    Proc. Natl. Acad. Sci. U.S.A. 91:11973-11977(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
    Mallet L., Bussereau F., Jacquet M.
    Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex."
    Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N.
    Mol. Cell. Biol. 18:6515-6524(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TOM COMPLEX.
  8. Cited for: FUNCTION, INTERACTION WITH TOM20 AND TOM70.
  9. "Multistep assembly of the protein import channel of the mitochondrial outer membrane."
    Model K., Meisinger C., Prinz T., Wiedemann N., Truscott K.N., Pfanner N., Ryan M.T.
    Nat. Struct. Biol. 8:361-370(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44 AND SER-46, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44 AND SER-46, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: INTERACTION WITH FCJ1.
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTOM22_YEAST
AccessioniPrimary (citable) accession number: P49334
Secondary accession number(s): D6W151, Q36757
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6610 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3