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Protein

Mitochondrial import receptor subunit TOM22

Gene

TOM22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM70 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Docks TOM20 and TOM70 for interaction with the general TOM40 import pore (GIP) complex. May regulate the TOM machinery organization, stability and channel gating.3 Publications

GO - Biological processi

  • protein import into mitochondrial matrix Source: SGD
  • protein import into mitochondrial outer membrane Source: SGD
  • protein targeting to mitochondrion Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33151-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM22
Alternative name(s):
Mitochondrial 17 kDa assembly protein
Mitochondrial 22 kDa outer membrane protein
Protein MAS17
Translocase of outer membrane 22 kDa subunit
Gene namesi
Name:TOM22
Synonyms:MAS17, MAS22, MOM22
Ordered Locus Names:YNL131W
ORF Names:N1217, N1862
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL131W.
SGDiS000005075. TOM22.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 97CytoplasmicSequence analysisAdd BLAST96
Transmembranei98 – 119HelicalSequence analysisAdd BLAST22
Topological domaini120 – 152Mitochondrial intermembraneSequence analysisAdd BLAST33

GO - Cellular componenti

  • integral component of mitochondrial outer membrane Source: SGD
  • mitochondrial outer membrane Source: Reactome
  • mitochondrial outer membrane translocase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000761132 – 152Mitochondrial import receptor subunit TOM22Add BLAST151

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei44PhosphoserineCombined sources1
Modified residuei46PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49334.
PRIDEiP49334.

PTM databases

iPTMnetiP49334.

Interactioni

Subunit structurei

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70). Interacts with TOM20 and TOM70. Interacts with FCJ1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BAXQ078123EBI-12527,EBI-516580From a different organism.
SAM50P539696EBI-12527,EBI-28646
TIM21P532202EBI-12527,EBI-23128
TOM20P351804EBI-12527,EBI-12522
TOM40P236448EBI-12527,EBI-12539

Protein-protein interaction databases

BioGridi35696. 63 interactors.
DIPiDIP-2302N.
IntActiP49334. 18 interactors.
MINTiMINT-478366.

Structurei

3D structure databases

ProteinModelPortaliP49334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Its cytoplasmic domain associates with the cytoplasmic domains of TOM20 and TOM70. Its intermembrane space domain provides a trans binding site for presequences and the single membrane anchor is required for a stable interaction between the GIP complex proteins.

Sequence similaritiesi

Belongs to the Tom22 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000206849.
InParanoidiP49334.
KOiK17769.
OMAiLTQIDDE.
OrthoDBiEOG092C5OM9.

Family and domain databases

InterProiIPR005683. Tom22.
IPR020951. Tom22_fungi.
[Graphical view]
PfamiPF04281. Tom22. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00986. 3a0801s05tom22. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVELTEIKDD VVQLDEPQFS RNQAIVEEKA SATNNDVVDD EDDSDSDFED
60 70 80 90 100
EFDENETLLD RIVALKDIVP PGKRQTISNF FGFTSSFVRN AFTKSGNLAW
110 120 130 140 150
TLTTTALLLG VPLSLSILAE QQLIEMEKTF DLQSDANNIL AQGEKDAAAT

AN
Length:152
Mass (Da):16,790
Last modified:January 23, 2007 - v3
Checksum:i6ACEC5D37D143CD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46843 Genomic DNA. Translation: CAA86894.1.
X82405 Genomic DNA. Translation: CAA57799.1.
X80348 Genomic DNA. Translation: CAA56588.1. Sequence problems.
Z71407 Genomic DNA. Translation: CAA96013.1.
BK006947 Genomic DNA. Translation: DAA10417.1.
PIRiS50250.
RefSeqiNP_014268.1. NM_001182969.1.

Genome annotation databases

EnsemblFungiiYNL131W; YNL131W; YNL131W.
GeneIDi855592.
KEGGisce:YNL131W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46843 Genomic DNA. Translation: CAA86894.1.
X82405 Genomic DNA. Translation: CAA57799.1.
X80348 Genomic DNA. Translation: CAA56588.1. Sequence problems.
Z71407 Genomic DNA. Translation: CAA96013.1.
BK006947 Genomic DNA. Translation: DAA10417.1.
PIRiS50250.
RefSeqiNP_014268.1. NM_001182969.1.

3D structure databases

ProteinModelPortaliP49334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35696. 63 interactors.
DIPiDIP-2302N.
IntActiP49334. 18 interactors.
MINTiMINT-478366.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

PTM databases

iPTMnetiP49334.

Proteomic databases

MaxQBiP49334.
PRIDEiP49334.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL131W; YNL131W; YNL131W.
GeneIDi855592.
KEGGisce:YNL131W.

Organism-specific databases

EuPathDBiFungiDB:YNL131W.
SGDiS000005075. TOM22.

Phylogenomic databases

HOGENOMiHOG000206849.
InParanoidiP49334.
KOiK17769.
OMAiLTQIDDE.
OrthoDBiEOG092C5OM9.

Enzyme and pathway databases

BioCyciYEAST:G3O-33151-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

PROiP49334.

Family and domain databases

InterProiIPR005683. Tom22.
IPR020951. Tom22_fungi.
[Graphical view]
PfamiPF04281. Tom22. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00986. 3a0801s05tom22. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTOM22_YEAST
AccessioniPrimary (citable) accession number: P49334
Secondary accession number(s): D6W151, Q36757
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6610 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.