Reviewed,
UniProtKB/Swiss-Prot P49334 (TOM22_YEAST)
Last modified
December 15, 2009.
Version 84.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Mitochondrial import receptor subunit TOM22 Alternative name(s): Mitochondrial 22 kDa outer membrane protein Translocase of outer membrane 22 kDa subunit Protein MAS17 Mitochondrial 17 kDa assembly protein | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 152 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM70 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Docks TOM20 and TOM70 for interaction with the general TOM40 import pore (GIP) complex. May regulate the TOM machinery organization, stability and channel gating. Ref.1 Ref.7 Ref.8 |
| Subunit structure | Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70). Interacts with TOM20 and TOM70. Ref.7 |
| Subcellular location | Mitochondrion outer membrane; Single-pass type II membrane protein. |
| Domain | Its cytoplasmic domain associates with the cytoplasmic domains of TOM20 and TOM70. Its intermembrane space domain provides a trans binding site for presequences and the single membrane anchor is required for a stable interaction between the GIP complex proteins. |
| Miscellaneous | Present with 6610 molecules/cell in log phase SD medium. Ref.9 |
| Sequence similarities | Belongs to the Tom22 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein transport Translocation Transport |
| Cellular component | Membrane Mitochondrion Mitochondrion outer membrane |
| Domain | Transmembrane |
| Molecular function | Receptor |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | protein import into mitochondrial matrix Ref.6 Ref.7 Inferred from mutant phenotype. Source: SGD protein import into mitochondrial outer membraneInferred from mutant phenotype. Source: SGD |
| Cellular component | integral to mitochondrial outer membrane Inferred from direct assay. Source: SGD mitochondrial outer membrane translocase complex Ref.6Inferred from direct assay. Source: SGD |
| Molecular function | P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from electronic annotation. Source: InterPro receptor activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| TIM21 | P53220 | 1 | EBI-12527,EBI-23128 | |
| TOM20 | P35180 | 1 | EBI-12527,EBI-12522 | |
| TOM40 | P23644 | 2 | EBI-12527,EBI-12539 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.1 | ||||||
| Chain | 2 – 152 | 151 | Mitochondrial import receptor subunit TOM22 | PRO_0000076113 | |||||
Regions | |||||||||
| Topological domain | 2 – 97 | 96 | Cytoplasmic Potential | ||||||
| Transmembrane | 98 – 119 | 22 | Potential | ||||||
| Topological domain | 120 – 152 | 33 | Mitochondrial intermembrane Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 44 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 46 | 1 | Phosphoserine Ref.10 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The mitochondrial receptor complex: Mom22 is essential for cell viability and directly interacts with preproteins." Hoenlinger A., Kuebrich M., Moczko M., Gaertner F., Mallet L., Bussereau F., Eckerskorn C., Lottspeich F., Dietmeier K., Jacquet M., Pfanner N. Mol. Cell. Biol. 15:3382-3389(1995) [PubMed: 7760834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-17, FUNCTION. Strain: ATCC 204508 / S288c. |
| [2] | "Identification of yeast MAS17 encoding the functional counterpart of the mitochondrial receptor complex protein MOM22 of Neurospora crassa." Nakai M., Endo T. FEBS Lett. 357:202-206(1995) [PubMed: 7805891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SP1. |
| [3] | "The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast." Lithgow T., Junne T., Suda K., Gratzer S., Schatz G. Proc. Natl. Acad. Sci. U.S.A. 91:11973-11977(1994) [PubMed: 7991567] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [4] | "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames." Mallet L., Bussereau F., Jacquet M. Yeast 11:1195-1209(1995) [PubMed: 8619318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [5] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J.Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [6] | "Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex." Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N. Mol. Cell. Biol. 18:6515-6524(1998) [PubMed: 9774667] [Abstract] Cited for: IDENTIFICATION IN THE TOM COMPLEX. |
| [7] | "Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase." van Wilpe S., Ryan M.T., Hill K., Maarse A.C., Meisinger C., Brix J., Dekker P.J.T., Moczko M., Wagner R., Meijer M., Guiard B., Hoenlinger A., Pfanner N. Nature 401:485-489(1999) [PubMed: 10519552] [Abstract] Cited for: FUNCTION, INTERACTION WITH TOM20 AND TOM70. |
| [8] | "Multistep assembly of the protein import channel of the mitochondrial outer membrane." Model K., Meisinger C., Prinz T., Wiedemann N., Truscott K.N., Pfanner N., Ryan M.T. Nat. Struct. Biol. 8:361-370(2001) [PubMed: 11276259] [Abstract] Cited for: FUNCTION. |
| [9] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [10] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44 AND SER-46, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Z46843 Genomic DNA. Translation: CAA86894.1. X82405 Genomic DNA. Translation: CAA57799.1. X80348 Genomic DNA. Translation: CAA56588.1. Sequence problems. Z71407 Genomic DNA. Translation: CAA96013.1. | |
| PIR | S50250. |
| RefSeq | NP_014268.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-2302N. |
| IntAct | P49334. 11 interactions. |
| STRING | P49334. |
Protein family/group databases | |
| TCDB | 3.A.8.1.1. mitochondrial protein translocase (MPT) family. |
Proteomic databases | |
| PeptideAtlas | P49334. |
| PRIDE | P49334. |
Genome annotation databases | |
| Ensembl | YNL131W; YNL131W; YNL131W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 855592. |
| KEGG | sce:YNL131W. |
| NMPDR | fig|4932.3.peg.5337. |
Organism-specific databases | |
| CYGD | YNL131w. |
| SGD | S000005075. TOM22. |
Phylogenomic databases | |
| HOGENOM | HBG737273. |
| OMA | QQLIEME. |
| OrthoDB | EOG9V44QQ. |
Gene expression databases | |
| ArrayExpress | P49334. |
| Genevestigator | P49334. |
| GermOnline | YNL131W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR005683. Tom22. [Graphical view] |
| Pfam | PF04281. Tom22. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 979738. |
Entry information
| Entry name | TOM22_YEAST | ||||||||
| Accession | Primary (citable) accession number: P49334 Secondary accession number(s): Q36757 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIV Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names |

Clusters with


