P49334 (TOM22_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import receptor subunit TOM22

Alternative name(s):
Mitochondrial 17 kDa assembly protein
Mitochondrial 22 kDa outer membrane protein
Protein MAS17
Translocase of outer membrane 22 kDa subunit

Gene names

Name:	TOM22
Synonyms:	MAS17, MAS22, MOM22
Ordered Locus Names:	YNL131W
ORF Names:	N1217, N1862

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM70 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Docks TOM20 and TOM70 for interaction with the general TOM40 import pore (GIP) complex. May regulate the TOM machinery organization, stability and channel gating. Ref.1 Ref.8 Ref.9
Subunit structure	Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70). Interacts with TOM20 and TOM70. Interacts with FCJ1. Ref.7 Ref.8 Ref.12
Subcellular location	Mitochondrion outer membrane; Single-pass type II membrane protein.
Domain	Its cytoplasmic domain associates with the cytoplasmic domains of TOM20 and TOM70. Its intermembrane space domain provides a trans binding site for presequences and the single membrane anchor is required for a stable interaction between the GIP complex proteins.
Miscellaneous	Present with 6610 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the Tom22 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein import into mitochondrial matrix Inferred from mutant phenotype Ref.8Ref.7. Source: SGD protein import into mitochondrial outer membrane Inferred from mutant phenotype PubMed 12628251. Source: SGD
Cellular_component	integral to mitochondrial outer membrane Inferred from direct assay PubMed 16689936. Source: SGD mitochondrial outer membrane translocase complex Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TIM21	P53220	2	EBI-12527,EBI-23128
TOM20	P35180	3	EBI-12527,EBI-12522
TOM40	P23644	5	EBI-12527,EBI-12539

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 152	151	Mitochondrial import receptor subunit TOM22		PRO_0000076113
Regions
	Topological domain	2 – 97	96	Cytoplasmic Potential
	Transmembrane	98 – 119	22	Helical; Potential
	Topological domain	120 – 152	33	Mitochondrial intermembrane Potential
Amino acid modifications
	Modified residue	44	1	Phosphoserine Ref.11
	Modified residue	46	1	Phosphoserine Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P49334 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 6ACEC5D37D143CD3	FASTA	152	16,790
	10 20 30 40 50 60 MVELTEIKDD VVQLDEPQFS RNQAIVEEKA SATNNDVVDD EDDSDSDFED EFDENETLLD 70 80 90 100 110 120 RIVALKDIVP PGKRQTISNF FGFTSSFVRN AFTKSGNLAW TLTTTALLLG VPLSLSILAE 130 140 150 QQLIEMEKTF DLQSDANNIL AQGEKDAAAT AN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mitochondrial receptor complex: Mom22 is essential for cell viability and directly interacts with preproteins." Hoenlinger A., Kuebrich M., Moczko M., Gaertner F., Mallet L., Bussereau F., Eckerskorn C., Lottspeich F., Dietmeier K., Jacquet M., Pfanner N. Mol. Cell. Biol. 15:3382-3389(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-17, FUNCTION. Strain: ATCC 204508 / S288c.
[2]	"Identification of yeast MAS17 encoding the functional counterpart of the mitochondrial receptor complex protein MOM22 of Neurospora crassa." Nakai M., Endo T. FEBS Lett. 357:202-206(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SP1.
[3]	"The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast." Lithgow T., Junne T., Suda K., Gratzer S., Schatz G. Proc. Natl. Acad. Sci. U.S.A. 91:11973-11977(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames." Mallet L., Bussereau F., Jacquet M. Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex." Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N. Mol. Cell. Biol. 18:6515-6524(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TOM COMPLEX.
[8]	"Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase." van Wilpe S., Ryan M.T., Hill K., Maarse A.C., Meisinger C., Brix J., Dekker P.J.T., Moczko M., Wagner R., Meijer M., Guiard B., Hoenlinger A., Pfanner N. Nature 401:485-489(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TOM20 AND TOM70.
[9]	"Multistep assembly of the protein import channel of the mitochondrial outer membrane." Model K., Meisinger C., Prinz T., Wiedemann N., Truscott K.N., Pfanner N., Ryan M.T. Nat. Struct. Biol. 8:361-370(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44 AND SER-46, MASS SPECTROMETRY.
[12]	"Dual role of mitofilin in mitochondrial membrane organization and protein biogenesis." von der Malsburg K., Muller J.M., Bohnert M., Oeljeklaus S., Kwiatkowska P., Becker T., Loniewska-Lwowska A., Wiese S., Rao S., Milenkovic D., Hutu D.P., Zerbes R.M., Schulze-Specking A., Meyer H.E., Martinou J.C., Rospert S., Rehling P., Meisinger C. , Veenhuis M., Warscheid B., van der Klei I.J., Pfanner N., Chacinska A., van der Laan M. Dev. Cell 21:694-707(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FCJ1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46843 Genomic DNA. Translation: CAA86894.1. X82405 Genomic DNA. Translation: CAA57799.1. X80348 Genomic DNA. Translation: CAA56588.1. Sequence problems. Z71407 Genomic DNA. Translation: CAA96013.1. BK006947 Genomic DNA. Translation: DAA10417.1.
PIR	S50250.
RefSeq	NP_014268.1. NM_001182969.1.
3D structure databases
ProteinModelPortal	P49334.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2302N.
IntAct	P49334. 9 interactions.
MINT	MINT-478366.
STRING	4932.YNL131W.
Protein family/group databases
TCDB	3.A.8.1.1. mitochondrial protein translocase (MPT) family.
Proteomic databases
PaxDb	P49334.
PeptideAtlas	P49334.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL131W; YNL131W; YNL131W.
GeneID	855592.
KEGG	sce:YNL131W.
Organism-specific databases
SGD	S000005075. TOM22.
Phylogenomic databases
eggNOG	NOG321322.
HOGENOM	HOG000206849.
OMA	MIKGANE.
OrthoDB	EOG42Z811.
Enzyme and pathway databases
Reactome	REACT_118590. Mitochondrial Protein Import (yeast). REACT_85873. Metabolism of proteins.
Gene expression databases
Genevestigator	P49334.
GermOnline	YNL131W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005683. Tom22. IPR020951. Tom22_fun. [Graphical view]
Pfam	PF04281. Tom22. 1 hit. [Graphical view]
TIGRFAMs	TIGR00986. 3a0801s05tom22. 1 hit.
ProtoNet	Search...
Other
NextBio	979738.

Entry information

Entry name

TOM22_YEAST

Accession

Primary (citable) accession number: P49334
Secondary accession number(s): D6W151, Q36757

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 112 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program