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P49334

- TOM22_YEAST

UniProt

P49334 - TOM22_YEAST

Protein

Mitochondrial import receptor subunit TOM22

Gene

TOM22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM70 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Docks TOM20 and TOM70 for interaction with the general TOM40 import pore (GIP) complex. May regulate the TOM machinery organization, stability and channel gating.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. protein import into mitochondrial matrix Source: SGD
    2. protein import into mitochondrial outer membrane Source: SGD

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33151-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.

    Protein family/group databases

    TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial import receptor subunit TOM22
    Alternative name(s):
    Mitochondrial 17 kDa assembly protein
    Mitochondrial 22 kDa outer membrane protein
    Protein MAS17
    Translocase of outer membrane 22 kDa subunit
    Gene namesi
    Name:TOM22
    Synonyms:MAS17, MAS22, MOM22
    Ordered Locus Names:YNL131W
    ORF Names:N1217, N1862
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    SGDiS000005075. TOM22.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of mitochondrial outer membrane Source: SGD
    2. mitochondrial outer membrane Source: Reactome
    3. mitochondrial outer membrane translocase complex Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 152151Mitochondrial import receptor subunit TOM22PRO_0000076113Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei44 – 441Phosphoserine2 Publications
    Modified residuei46 – 461Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP49334.
    PaxDbiP49334.
    PeptideAtlasiP49334.

    Expressioni

    Gene expression databases

    GenevestigatoriP49334.

    Interactioni

    Subunit structurei

    Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70). Interacts with TOM20 and TOM70. Interacts with FCJ1.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BAXQ078123EBI-12527,EBI-516580From a different organism.
    SAM50P539696EBI-12527,EBI-28646
    TIM21P532202EBI-12527,EBI-23128
    TOM20P351804EBI-12527,EBI-12522
    TOM40P236448EBI-12527,EBI-12539

    Protein-protein interaction databases

    BioGridi35696. 64 interactions.
    DIPiDIP-2302N.
    IntActiP49334. 17 interactions.
    MINTiMINT-478366.
    STRINGi4932.YNL131W.

    Structurei

    3D structure databases

    ProteinModelPortaliP49334.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 9796CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini120 – 15233Mitochondrial intermembraneSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei98 – 11922HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    Its cytoplasmic domain associates with the cytoplasmic domains of TOM20 and TOM70. Its intermembrane space domain provides a trans binding site for presequences and the single membrane anchor is required for a stable interaction between the GIP complex proteins.

    Sequence similaritiesi

    Belongs to the Tom22 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG321322.
    HOGENOMiHOG000206849.
    KOiK17769.
    OMAiLTQIDDE.
    OrthoDBiEOG7D8694.

    Family and domain databases

    InterProiIPR005683. Tom22.
    IPR020951. Tom22_fun.
    [Graphical view]
    PfamiPF04281. Tom22. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00986. 3a0801s05tom22. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49334-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVELTEIKDD VVQLDEPQFS RNQAIVEEKA SATNNDVVDD EDDSDSDFED    50
    EFDENETLLD RIVALKDIVP PGKRQTISNF FGFTSSFVRN AFTKSGNLAW 100
    TLTTTALLLG VPLSLSILAE QQLIEMEKTF DLQSDANNIL AQGEKDAAAT 150
    AN 152
    Length:152
    Mass (Da):16,790
    Last modified:January 23, 2007 - v3
    Checksum:i6ACEC5D37D143CD3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46843 Genomic DNA. Translation: CAA86894.1.
    X82405 Genomic DNA. Translation: CAA57799.1.
    X80348 Genomic DNA. Translation: CAA56588.1. Sequence problems.
    Z71407 Genomic DNA. Translation: CAA96013.1.
    BK006947 Genomic DNA. Translation: DAA10417.1.
    PIRiS50250.
    RefSeqiNP_014268.1. NM_001182969.1.

    Genome annotation databases

    EnsemblFungiiYNL131W; YNL131W; YNL131W.
    GeneIDi855592.
    KEGGisce:YNL131W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46843 Genomic DNA. Translation: CAA86894.1 .
    X82405 Genomic DNA. Translation: CAA57799.1 .
    X80348 Genomic DNA. Translation: CAA56588.1 . Sequence problems.
    Z71407 Genomic DNA. Translation: CAA96013.1 .
    BK006947 Genomic DNA. Translation: DAA10417.1 .
    PIRi S50250.
    RefSeqi NP_014268.1. NM_001182969.1.

    3D structure databases

    ProteinModelPortali P49334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35696. 64 interactions.
    DIPi DIP-2302N.
    IntActi P49334. 17 interactions.
    MINTi MINT-478366.
    STRINGi 4932.YNL131W.

    Protein family/group databases

    TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Proteomic databases

    MaxQBi P49334.
    PaxDbi P49334.
    PeptideAtlasi P49334.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL131W ; YNL131W ; YNL131W .
    GeneIDi 855592.
    KEGGi sce:YNL131W.

    Organism-specific databases

    SGDi S000005075. TOM22.

    Phylogenomic databases

    eggNOGi NOG321322.
    HOGENOMi HOG000206849.
    KOi K17769.
    OMAi LTQIDDE.
    OrthoDBi EOG7D8694.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33151-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.

    Miscellaneous databases

    NextBioi 979738.

    Gene expression databases

    Genevestigatori P49334.

    Family and domain databases

    InterProi IPR005683. Tom22.
    IPR020951. Tom22_fun.
    [Graphical view ]
    Pfami PF04281. Tom22. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00986. 3a0801s05tom22. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The mitochondrial receptor complex: Mom22 is essential for cell viability and directly interacts with preproteins."
      Hoenlinger A., Kuebrich M., Moczko M., Gaertner F., Mallet L., Bussereau F., Eckerskorn C., Lottspeich F., Dietmeier K., Jacquet M., Pfanner N.
      Mol. Cell. Biol. 15:3382-3389(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-17, FUNCTION.
      Strain: ATCC 204508 / S288c.
    2. "Identification of yeast MAS17 encoding the functional counterpart of the mitochondrial receptor complex protein MOM22 of Neurospora crassa."
      Nakai M., Endo T.
      FEBS Lett. 357:202-206(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SP1.
    3. "The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast."
      Lithgow T., Junne T., Suda K., Gratzer S., Schatz G.
      Proc. Natl. Acad. Sci. U.S.A. 91:11973-11977(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
      Mallet L., Bussereau F., Jacquet M.
      Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. "Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex."
      Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N.
      Mol. Cell. Biol. 18:6515-6524(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TOM COMPLEX.
    8. Cited for: FUNCTION, INTERACTION WITH TOM20 AND TOM70.
    9. "Multistep assembly of the protein import channel of the mitochondrial outer membrane."
      Model K., Meisinger C., Prinz T., Wiedemann N., Truscott K.N., Pfanner N., Ryan M.T.
      Nat. Struct. Biol. 8:361-370(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44 AND SER-46, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44 AND SER-46, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: INTERACTION WITH FCJ1.
    14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTOM22_YEAST
    AccessioniPrimary (citable) accession number: P49334
    Secondary accession number(s): D6W151, Q36757
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 125 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6610 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3