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Protein

Ethylene receptor 1

Gene

ETR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ethylene receptor related to bacterial two-component regulators. Acts as a redundant negative regulator of ethylene signaling (PubMed:15466228, PubMed:15703053). In the presence of ethylene, the auto-kinase activity of ETR1 is inhibited and the non-phosphorylated kinase domain binds tightly to the corresponding domain of EIN2 (PubMed:20591837).3 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Cu cation1 PublicationNote: Binds 1 copper ion per dimer.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi65Copper1
Metal bindingi69Copper1
Binding sitei513ADPCombined sources1
Binding sitei529ADPCombined sources1
Binding sitei544ADP; via carbonyl oxygenCombined sources1
Binding sitei548ADP; via amide nitrogenCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi470 – 473ADPCombined sources4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ethylene binding Source: TAIR
  • ethylene receptor activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: InterPro
  • protein histidine kinase activity Source: TAIR

GO - Biological processi

  • cell division Source: TAIR
  • cellular response to iron ion Source: TAIR
  • cytokinin metabolic process Source: TAIR
  • defense response Source: TAIR
  • defense response by callose deposition in cell wall Source: TAIR
  • defense response to bacterium Source: TAIR
  • detection of ethylene stimulus Source: TAIR
  • hydrogen peroxide biosynthetic process Source: TAIR
  • negative regulation of ethylene-activated signaling pathway Source: TAIR
  • regulation of seedling development Source: TAIR
  • regulation of stomatal movement Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to auxin Source: TAIR
  • response to ethylene Source: TAIR
  • response to gibberellin Source: TAIR
  • response to heat Source: TAIR
  • response to insect Source: TAIR
  • response to molecule of bacterial origin Source: TAIR
  • response to salt stress Source: TAIR
  • sugar mediated signaling pathway Source: TAIR
  • vasculature development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Transferase

Keywords - Biological processi

Ethylene signaling pathway, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Copper, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.13.3. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene receptor 11 Publication (EC:2.7.13.3Curated)
Short name:
AtETR11 Publication
Alternative name(s):
Protein ETHYLENE RESPONSE 11 Publication
Protein ETR11 Publication
Gene namesi
Name:ETR11 Publication
Ordered Locus Names:At1g66340Imported
ORF Names:T27F4.9Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G66340.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Transmembranei92 – 112HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype in ethylene response; due to redundancy with ERS1. Ers1 and etr1 double mutants display a constitutive ethylene-response phenotype.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi4C → S: Prevents dimerization but not ethylene binding. 2 Publications1
Mutagenesisi6C → S: Prevents dimerization but not ethylene binding. 2 Publications1
Mutagenesisi31A → V in etr1-3; ethylene insensitivity. 1
Mutagenesisi38E → A: No effect on ethylene binding. 1 Publication1
Mutagenesisi62I → F in etr1-4; ethylene insensitivity. 1
Mutagenesisi65C → Y or S in etr1-1; no copper binding and ethylene insensitivity. 2 Publications1
Mutagenesisi69H → A: No copper binding and ethylene insensitivity. 1 Publication1
Mutagenesisi79H → A: No effect. 1 Publication1
Mutagenesisi87M → A: No effect on ethylene binding. 1 Publication1
Mutagenesisi99C → S: No effect on dimerization or ethylene binding. 2 Publications1
Mutagenesisi102A → T in etr1-2; ethylene insensitivity. 1
Mutagenesisi104M → A: No effect on ethylene binding. 1 Publication1
Mutagenesisi107H → A: No effect on ethylene binding. 1 Publication1
Mutagenesisi353H → A: Loss of phosphorylation and increased affinity toward EIN2. 1 Publication1
Mutagenesisi353H → E: Reduced affinity toward EIN2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000814121 – 738Ethylene receptor 1Add BLAST738

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi4Interchain1 Publication
Disulfide bondi6Interchain1 Publication
Modified residuei353Phosphohistidine; by autocatalysis1 Publication1
Modified residuei6594-aspartylphosphatePROSITE-ProRule annotation1
Cross-linki714Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Autophosphorylated. Phosphorylation at His-353 modulates the interaction with EIN2.1 Publication

Keywords - PTMi

Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP49333.

PTM databases

iPTMnetiP49333.

Expressioni

Tissue specificityi

Leaves, roots, stems, seedlings, flowers, anthers, carpels and ovules.1 Publication

Gene expression databases

GenevisibleiP49333. AT.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Heteromer with ERS1, ERS2, ETR2 and EIN4. Interacts with AHP1, AHP2 and AHP3. Interacts with RTE1 (PubMed:10930573, PubMed:18577522, PubMed:20952388, PubMed:7759498). Interacts with EIN2 (PubMed:19769567).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1606682,EBI-1606682
AHP1Q9ZNV93EBI-1606682,EBI-1100673
CTR1Q056096EBI-1606682,EBI-1606697
EIN2Q9S8142EBI-1606682,EBI-2437287
ERS2P938252EBI-1606682,EBI-1787556
ETR2Q0WPQ22EBI-1606682,EBI-1787533
RTE1F4ITL65EBI-1606682,EBI-2437263

Protein-protein interaction databases

BioGridi28172. 45 interactors.
IntActiP49333. 11 interactors.
MINTiMINT-8068106.
STRINGi3702.AT1G66340.1.

Structurei

Secondary structure

1738
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi415 – 418Combined sources4
Helixi419 – 437Combined sources19
Beta strandi441 – 445Combined sources5
Beta strandi451 – 455Combined sources5
Helixi457 – 474Combined sources18
Beta strandi476 – 478Combined sources3
Beta strandi480 – 487Combined sources8
Beta strandi504 – 512Combined sources9
Helixi520 – 525Combined sources6
Helixi547 – 557Combined sources11
Beta strandi561 – 565Combined sources5
Beta strandi574 – 583Combined sources10
Beta strandi611 – 615Combined sources5
Helixi619 – 631Combined sources13
Beta strandi635 – 641Combined sources7
Helixi642 – 648Combined sources7
Beta strandi654 – 659Combined sources6
Turni664 – 668Combined sources5
Helixi669 – 678Combined sources10
Beta strandi687 – 693Combined sources7
Helixi697 – 705Combined sources9
Beta strandi710 – 715Combined sources6
Helixi718 – 729Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DCFX-ray2.50A605-738[»]
4PL9X-ray1.90A407-589[»]
ProteinModelPortaliP49333.
SMRiP49333.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49333.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini158 – 307GAFAdd BLAST150
Domaini350 – 585Histidine kinasePROSITE-ProRule annotationAdd BLAST236
Domaini611 – 729Response regulatoryPROSITE-ProRule annotationAdd BLAST119

Domaini

The GAF domain is sufficient to mediate heteromerization.

Sequence similaritiesi

Belongs to the ethylene receptor family.Curated
Contains 1 GAF domain.Curated
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0519. Eukaryota.
COG0642. LUCA.
HOGENOMiHOG000240343.
InParanoidiP49333.
KOiK14509.
OMAiEGNIWIE.
OrthoDBiEOG093605FG.
PhylomeDBiP49333.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR014525. ETR.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF026389. Ethyln_sen_HK. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVCNCIEPQ WPADELLMKY QYISDFFIAI AYFSIPLELI YFVKKSAVFP
60 70 80 90 100
YRWVLVQFGA FIVLCGATHL INLWTFTTHS RTVALVMTTA KVLTAVVSCA
110 120 130 140 150
TALMLVHIIP DLLSVKTREL FLKNKAAELD REMGLIRTQE ETGRHVRMLT
160 170 180 190 200
HEIRSTLDRH TILKTTLVEL GRTLALEECA LWMPTRTGLE LQLSYTLRHQ
210 220 230 240 250
HPVEYTVPIQ LPVINQVFGT SRAVKISPNS PVARLRPVSG KYMLGEVVAV
260 270 280 290 300
RVPLLHLSNF QINDWPELST KRYALMVLML PSDSARQWHV HELELVEVVA
310 320 330 340 350
DQVAVALSHA AILEESMRAR DLLMEQNVAL DLARREAETA IRARNDFLAV
360 370 380 390 400
MNHEMRTPMH AIIALSSLLQ ETELTPEQRL MVETILKSSN LLATLMNDVL
410 420 430 440 450
DLSRLEDGSL QLELGTFNLH TLFREVLNLI KPIAVVKKLP ITLNLAPDLP
460 470 480 490 500
EFVVGDEKRL MQIILNIVGN AVKFSKQGSI SVTALVTKSD TRAADFFVVP
510 520 530 540 550
TGSHFYLRVK VKDSGAGINP QDIPKIFTKF AQTQSLATRS SGGSGLGLAI
560 570 580 590 600
SKRFVNLMEG NIWIESDGLG KGCTAIFDVK LGISERSNES KQSGIPKVPA
610 620 630 640 650
IPRHSNFTGL KVLVMDENGV SRMVTKGLLV HLGCEVTTVS SNEECLRVVS
660 670 680 690 700
HEHKVVFMDV CMPGVENYQI ALRIHEKFTK QRHQRPLLVA LSGNTDKSTK
710 720 730
EKCMSFGLDG VLLKPVSLDN IRDVLSDLLE PRVLYEGM
Length:738
Mass (Da):82,566
Last modified:February 1, 1996 - v1
Checksum:iD6ED3C4BBA87E96E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24119 Genomic DNA. Translation: AAA70047.1.
AC020665 Genomic DNA. Translation: AAG52169.1.
CP002684 Genomic DNA. Translation: AEE34497.1.
PIRiA48246.
RefSeqiNP_176808.3. NM_105305.4.
UniGeneiAt.43238.

Genome annotation databases

EnsemblPlantsiAT1G66340.1; AT1G66340.1; AT1G66340.
GeneIDi842951.
GrameneiAT1G66340.1; AT1G66340.1; AT1G66340.
KEGGiath:AT1G66340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24119 Genomic DNA. Translation: AAA70047.1.
AC020665 Genomic DNA. Translation: AAG52169.1.
CP002684 Genomic DNA. Translation: AEE34497.1.
PIRiA48246.
RefSeqiNP_176808.3. NM_105305.4.
UniGeneiAt.43238.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DCFX-ray2.50A605-738[»]
4PL9X-ray1.90A407-589[»]
ProteinModelPortaliP49333.
SMRiP49333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28172. 45 interactors.
IntActiP49333. 11 interactors.
MINTiMINT-8068106.
STRINGi3702.AT1G66340.1.

PTM databases

iPTMnetiP49333.

Proteomic databases

PaxDbiP49333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G66340.1; AT1G66340.1; AT1G66340.
GeneIDi842951.
GrameneiAT1G66340.1; AT1G66340.1; AT1G66340.
KEGGiath:AT1G66340.

Organism-specific databases

TAIRiAT1G66340.

Phylogenomic databases

eggNOGiKOG0519. Eukaryota.
COG0642. LUCA.
HOGENOMiHOG000240343.
InParanoidiP49333.
KOiK14509.
OMAiEGNIWIE.
OrthoDBiEOG093605FG.
PhylomeDBiP49333.

Enzyme and pathway databases

BRENDAi2.7.13.3. 399.

Miscellaneous databases

EvolutionaryTraceiP49333.
PROiP49333.

Gene expression databases

GenevisibleiP49333. AT.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR014525. ETR.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF026389. Ethyln_sen_HK. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiETR1_ARATH
AccessioniPrimary (citable) accession number: P49333
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 164 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.