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Protein

Ethylene receptor 1

Gene

ETR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ethylene receptor related to bacterial two-component regulators. Acts as a redundant negative regulator of ethylene signaling (PubMed:15466228, PubMed:15703053). In the presence of ethylene, the auto-kinase activity of ETR1 is inhibited and the non-phosphorylated kinase domain binds tightly to the corresponding domain of EIN2 (PubMed:20591837).3 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Cu cation1 PublicationNote: Binds 1 copper ion per dimer.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi65 – 651Copper
Metal bindingi69 – 691Copper
Binding sitei513 – 5131ADPCombined sources
Binding sitei529 – 5291ADPCombined sources
Binding sitei544 – 5441ADP; via carbonyl oxygenCombined sources
Binding sitei548 – 5481ADP; via amide nitrogenCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi470 – 4734ADPCombined sources

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ethylene binding Source: TAIR
  • ethylene receptor activity Source: UniProtKB
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: InterPro
  • protein histidine kinase activity Source: TAIR

GO - Biological processi

  • cell division Source: TAIR
  • cellular response to iron ion Source: TAIR
  • cytokinin metabolic process Source: TAIR
  • defense response Source: TAIR
  • defense response by callose deposition in cell wall Source: TAIR
  • defense response to bacterium Source: TAIR
  • detection of ethylene stimulus Source: TAIR
  • hydrogen peroxide biosynthetic process Source: TAIR
  • negative regulation of ethylene-activated signaling pathway Source: TAIR
  • regulation of seedling development Source: TAIR
  • regulation of stomatal movement Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to auxin Source: TAIR
  • response to ethylene Source: TAIR
  • response to gibberellin Source: TAIR
  • response to heat Source: TAIR
  • response to insect Source: TAIR
  • response to molecule of bacterial origin Source: TAIR
  • response to salt stress Source: TAIR
  • sugar mediated signaling pathway Source: TAIR
  • vasculature development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Transferase

Keywords - Biological processi

Ethylene signaling pathway, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Copper, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G66340-MONOMER.
BRENDAi2.7.13.3. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene receptor 11 Publication (EC:2.7.13.3Curated)
Short name:
AtETR11 Publication
Alternative name(s):
Protein ETHYLENE RESPONSE 11 Publication
Protein ETR11 Publication
Gene namesi
Name:ETR11 Publication
Ordered Locus Names:At1g66340Imported
ORF Names:T27F4.9Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G66340.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 4321HelicalSequence analysisAdd
BLAST
Transmembranei53 – 7321HelicalSequence analysisAdd
BLAST
Transmembranei92 – 11221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype in ethylene response; due to redundancy with ERS1. Ers1 and etr1 double mutants display a constitutive ethylene-response phenotype.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi4 – 41C → S: Prevents dimerization but not ethylene binding. 2 Publications
Mutagenesisi6 – 61C → S: Prevents dimerization but not ethylene binding. 2 Publications
Mutagenesisi31 – 311A → V in etr1-3; ethylene insensitivity.
Mutagenesisi38 – 381E → A: No effect on ethylene binding. 1 Publication
Mutagenesisi62 – 621I → F in etr1-4; ethylene insensitivity.
Mutagenesisi65 – 651C → Y or S in etr1-1; no copper binding and ethylene insensitivity. 2 Publications
Mutagenesisi69 – 691H → A: No copper binding and ethylene insensitivity. 1 Publication
Mutagenesisi79 – 791H → A: No effect. 1 Publication
Mutagenesisi87 – 871M → A: No effect on ethylene binding. 1 Publication
Mutagenesisi99 – 991C → S: No effect on dimerization or ethylene binding. 2 Publications
Mutagenesisi102 – 1021A → T in etr1-2; ethylene insensitivity.
Mutagenesisi104 – 1041M → A: No effect on ethylene binding. 1 Publication
Mutagenesisi107 – 1071H → A: No effect on ethylene binding. 1 Publication
Mutagenesisi353 – 3531H → A: Loss of phosphorylation and increased affinity toward EIN2. 1 Publication
Mutagenesisi353 – 3531H → E: Reduced affinity toward EIN2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 738738Ethylene receptor 1PRO_0000081412Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi4 – 4Interchain1 Publication
Disulfide bondi6 – 6Interchain1 Publication
Modified residuei353 – 3531Phosphohistidine; by autocatalysis1 Publication
Modified residuei659 – 65914-aspartylphosphatePROSITE-ProRule annotation
Cross-linki714 – 714Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Autophosphorylated. Phosphorylation at His-353 modulates the interaction with EIN2.1 Publication

Keywords - PTMi

Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP49333.
PRIDEiP49333.

PTM databases

iPTMnetiP49333.

Expressioni

Tissue specificityi

Leaves, roots, stems, seedlings, flowers, anthers, carpels and ovules.1 Publication

Gene expression databases

GenevisibleiP49333. AT.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Heteromer with ERS1, ERS2, ETR2 and EIN4. Interacts with AHP1, AHP2 and AHP3. Interacts with RTE1 (PubMed:10930573, PubMed:18577522, PubMed:20952388, PubMed:7759498). Interacts with EIN2 (PubMed:19769567).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1606682,EBI-1606682
AHP1Q9ZNV93EBI-1606682,EBI-1100673
CTR1Q056096EBI-1606682,EBI-1606697
EIN2Q9S8142EBI-1606682,EBI-2437287
ERS2P938252EBI-1606682,EBI-1787556
ETR2Q0WPQ22EBI-1606682,EBI-1787533
RTE1F4ITL65EBI-1606682,EBI-2437263

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi28172. 45 interactions.
IntActiP49333. 11 interactions.
MINTiMINT-8068106.
STRINGi3702.AT1G66340.1.

Structurei

Secondary structure

1
738
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi415 – 4184Combined sources
Helixi419 – 43719Combined sources
Beta strandi441 – 4455Combined sources
Beta strandi451 – 4555Combined sources
Helixi457 – 47418Combined sources
Beta strandi476 – 4783Combined sources
Beta strandi480 – 4878Combined sources
Beta strandi504 – 5129Combined sources
Helixi520 – 5256Combined sources
Helixi547 – 55711Combined sources
Beta strandi561 – 5655Combined sources
Beta strandi574 – 58310Combined sources
Beta strandi611 – 6155Combined sources
Helixi619 – 63113Combined sources
Beta strandi635 – 6417Combined sources
Helixi642 – 6487Combined sources
Beta strandi654 – 6596Combined sources
Turni664 – 6685Combined sources
Helixi669 – 67810Combined sources
Beta strandi687 – 6937Combined sources
Helixi697 – 7059Combined sources
Beta strandi710 – 7156Combined sources
Helixi718 – 72912Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DCFX-ray2.50A605-738[»]
4PL9X-ray1.90A407-589[»]
ProteinModelPortaliP49333.
SMRiP49333. Positions 308-584, 605-736.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49333.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini158 – 307150GAFAdd
BLAST
Domaini350 – 585236Histidine kinasePROSITE-ProRule annotationAdd
BLAST
Domaini611 – 729119Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Domaini

The GAF domain is sufficient to mediate heteromerization.

Sequence similaritiesi

Belongs to the ethylene receptor family.Curated
Contains 1 GAF domain.Curated
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0519. Eukaryota.
COG0642. LUCA.
HOGENOMiHOG000240343.
InParanoidiP49333.
KOiK14509.
OMAiEGNIWIE.
OrthoDBiEOG093605FG.
PhylomeDBiP49333.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR014525. ETR.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF026389. Ethyln_sen_HK. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVCNCIEPQ WPADELLMKY QYISDFFIAI AYFSIPLELI YFVKKSAVFP
60 70 80 90 100
YRWVLVQFGA FIVLCGATHL INLWTFTTHS RTVALVMTTA KVLTAVVSCA
110 120 130 140 150
TALMLVHIIP DLLSVKTREL FLKNKAAELD REMGLIRTQE ETGRHVRMLT
160 170 180 190 200
HEIRSTLDRH TILKTTLVEL GRTLALEECA LWMPTRTGLE LQLSYTLRHQ
210 220 230 240 250
HPVEYTVPIQ LPVINQVFGT SRAVKISPNS PVARLRPVSG KYMLGEVVAV
260 270 280 290 300
RVPLLHLSNF QINDWPELST KRYALMVLML PSDSARQWHV HELELVEVVA
310 320 330 340 350
DQVAVALSHA AILEESMRAR DLLMEQNVAL DLARREAETA IRARNDFLAV
360 370 380 390 400
MNHEMRTPMH AIIALSSLLQ ETELTPEQRL MVETILKSSN LLATLMNDVL
410 420 430 440 450
DLSRLEDGSL QLELGTFNLH TLFREVLNLI KPIAVVKKLP ITLNLAPDLP
460 470 480 490 500
EFVVGDEKRL MQIILNIVGN AVKFSKQGSI SVTALVTKSD TRAADFFVVP
510 520 530 540 550
TGSHFYLRVK VKDSGAGINP QDIPKIFTKF AQTQSLATRS SGGSGLGLAI
560 570 580 590 600
SKRFVNLMEG NIWIESDGLG KGCTAIFDVK LGISERSNES KQSGIPKVPA
610 620 630 640 650
IPRHSNFTGL KVLVMDENGV SRMVTKGLLV HLGCEVTTVS SNEECLRVVS
660 670 680 690 700
HEHKVVFMDV CMPGVENYQI ALRIHEKFTK QRHQRPLLVA LSGNTDKSTK
710 720 730
EKCMSFGLDG VLLKPVSLDN IRDVLSDLLE PRVLYEGM
Length:738
Mass (Da):82,566
Last modified:February 1, 1996 - v1
Checksum:iD6ED3C4BBA87E96E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24119 Genomic DNA. Translation: AAA70047.1.
AC020665 Genomic DNA. Translation: AAG52169.1.
CP002684 Genomic DNA. Translation: AEE34497.1.
PIRiA48246.
RefSeqiNP_176808.3. NM_105305.4.
UniGeneiAt.43238.

Genome annotation databases

EnsemblPlantsiAT1G66340.1; AT1G66340.1; AT1G66340.
GeneIDi842951.
GrameneiAT1G66340.1; AT1G66340.1; AT1G66340.
KEGGiath:AT1G66340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24119 Genomic DNA. Translation: AAA70047.1.
AC020665 Genomic DNA. Translation: AAG52169.1.
CP002684 Genomic DNA. Translation: AEE34497.1.
PIRiA48246.
RefSeqiNP_176808.3. NM_105305.4.
UniGeneiAt.43238.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DCFX-ray2.50A605-738[»]
4PL9X-ray1.90A407-589[»]
ProteinModelPortaliP49333.
SMRiP49333. Positions 308-584, 605-736.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28172. 45 interactions.
IntActiP49333. 11 interactions.
MINTiMINT-8068106.
STRINGi3702.AT1G66340.1.

PTM databases

iPTMnetiP49333.

Proteomic databases

PaxDbiP49333.
PRIDEiP49333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G66340.1; AT1G66340.1; AT1G66340.
GeneIDi842951.
GrameneiAT1G66340.1; AT1G66340.1; AT1G66340.
KEGGiath:AT1G66340.

Organism-specific databases

TAIRiAT1G66340.

Phylogenomic databases

eggNOGiKOG0519. Eukaryota.
COG0642. LUCA.
HOGENOMiHOG000240343.
InParanoidiP49333.
KOiK14509.
OMAiEGNIWIE.
OrthoDBiEOG093605FG.
PhylomeDBiP49333.

Enzyme and pathway databases

BioCyciARA:AT1G66340-MONOMER.
BRENDAi2.7.13.3. 399.

Miscellaneous databases

EvolutionaryTraceiP49333.
PROiP49333.

Gene expression databases

GenevisibleiP49333. AT.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR014525. ETR.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF026389. Ethyln_sen_HK. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiETR1_ARATH
AccessioniPrimary (citable) accession number: P49333
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.