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P49331 (GTFD_STRMU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucosyltransferase-S

Short name=GTF-S
EC=2.4.1.5
Alternative name(s):
Dextransucrase
Sucrose 6-glucosyltransferase
Gene names
Name:gtfD
Ordered Locus Names:SMU_910
OrganismStreptococcus mutans serotype c (strain ATCC 700610 / UA159) [Complete proteome] [HAMAP]
Taxonomic identifier210007 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length1462 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

Catalytic activity

Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).

Subcellular location

Secreted.

Miscellaneous

GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 70 family.

Contains 14 cell wall-binding repeats.

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseDental caries
   DomainRepeat
Signal
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglucan biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondextransucrase activity

Inferred from electronic annotation. Source: UniProtKB-EC

glucosyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – ? Potential
Chain? – 1462Glucosyltransferase-SPRO_0000021387

Regions

Repeat171 – 19020Cell wall-binding 1
Repeat192 – 21120Cell wall-binding 2
Repeat1095 – 111521Cell wall-binding 3
Repeat1116 – 113621Cell wall-binding 4
Repeat1137 – 115620Cell wall-binding 5
Repeat1180 – 120122Cell wall-binding 6
Repeat1202 – 122120Cell wall-binding 7
Repeat1223 – 124422Cell wall-binding 8
Repeat1246 – 126621Cell wall-binding 9
Repeat1267 – 128620Cell wall-binding 10
Repeat1310 – 133021Cell wall-binding 11
Repeat1331 – 135020Cell wall-binding 12
Repeat1352 – 137221Cell wall-binding 13
Repeat1374 – 139421Cell wall-binding 14

Natural variations

Natural variant101Y → H in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
Natural variant191I → V in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
Natural variant581K → E in strain: MT4467.
Natural variant681A → S in strain: MT4239 and MT4245.
Natural variant811A → T in strain: MT4251 and MT8148.
Natural variant1131T → I in strain: MT4239 and MT4245.
Natural variant1221A → V in strain: MT4239, MT4245 and MT8148.
Natural variant1321A → S in strain: GS-5 and MT4467.
Natural variant1351A → V in strain: MT4245.
Natural variant1371A → T in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
Natural variant2021V → L in strain: MT4239.
Natural variant2551D → N in strain: MT8148.
Natural variant2751E → D in strain: MT4239, MT4245 and MT4251.
Natural variant2881D → N in strain: MT4239, MT4245 and MT4251.
Natural variant3011Q → H in strain: MT4245.
Natural variant3131D → N in strain: MT4239 and MT4251.
Natural variant3171E → K in strain: MT4239.
Natural variant3281V → F in strain: MT4239.
Natural variant3501F → L in strain: MT4239, MT4251 and MT4467.
Natural variant628 – 6336KKKYTQ → EKEYTL in strain: MT4251.
Natural variant6881A → S in strain: MT4239.
Natural variant726 – 7327TDQGSEA → ADKGNDS in strain: MT4251.
Natural variant726 – 7305TDQGS → ADKGN in strain: MT4239 and MT4245.
Natural variant7621T → A in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
Natural variant9641D → Y in strain: MT4251.
Natural variant10191E → K in strain: MT4245 and MT4251.
Natural variant1059 – 10602LG → IR in strain: MT4251.
Natural variant10601G → R in strain: MT4245.
Natural variant10801G → R in strain: MT4239.
Natural variant11421H → Q in strain: GS-5.
Natural variant11981S → N in strain: MT4239.
Natural variant12201Y → C in strain: MT4251 and MT4467.
Natural variant12801F → L in strain: MT4467.
Natural variant12821Q → P in strain: MT4245.
Natural variant12901K → T in strain: MT4245.
Natural variant13111N → D in strain: MT4245.
Natural variant14031G → D in strain: GS-5 and MT4467.
Natural variant14251G → R in strain: GS-5.
Natural variant14491R → K in strain: MT4467.

Experimental info

Sequence conflict1428 – 146235RYYDK…IARYY → VYR in AAA26895. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P49331 [UniParc].

Last modified November 28, 2002. Version 3.
Checksum: CE4A279C4D708645

FASTA1,462163,388
        10         20         30         40         50         60 
METKRRYKMY KVKKHWVTIA VASGLITLGT TTLGSSVSAE TEQQTSDKVV TQKSEDDKAA 

        70         80         90        100        110        120 
SESSQTDAPK TKQAQTEQTQ AQSQANVADT STSITKETPS QNITTQANSD DKTVTNTKSE 

       130        140        150        160        170        180 
EAQTSEERTK QAEEAQATAS SQALTQAKAE LTKQRQTAAQ ENKNPVDLAA IPNVKQIDGK 

       190        200        210        220        230        240 
YYYIGSDGQP KKNFALTVNN KVLYFDKNTG ALTDTSQYQF KQGLTKLNND YTPHNQIVNF 

       250        260        270        280        290        300 
ENTSLETIDN YVTADSWYRP KDILKNGKTW TASSESDLRP LLMSWWPDKQ TQIAYLNYMN 

       310        320        330        340        350        360 
QQGLGTGENY TADSSQESLN LAAQTVQVKI ETKISQTQQT QWLRDIINSF VKTQPNWNSQ 

       370        380        390        400        410        420 
TESDTSAGEK DHLQGGALLY SNSDKTAYAN SDYRLLNRTP TSQTGKPKYF EDNSSGGYDF 

       430        440        450        460        470        480 
LLANDIDNSN PVVQAEQLNW LHYLMNYGSI VANDPEANFD GVRVDAVDNV NADLLQIASD 

       490        500        510        520        530        540 
YLKAHYGVDK SEKNAINHLS ILEAWSDNDP QYNKDTKGAQ LPIDNKLRLS LLYALTRPLE 

       550        560        570        580        590        600 
KDASNKNEIR SGLEPVITNS LNNRSAEGKN SERMANYIFI RAHDSEVQTV IAKIIKAQIN 

       610        620        630        640        650        660 
PKTDGLTFTL DELKQAFKIY NEDMRQAKKK YTQSNIPTAY ALMLSNKDSI TRLYYGDMYS 

       670        680        690        700        710        720 
DDGQYMATKS PYYDAIDTLL KARIKYAAGG QDMKITYVEG DKSHMDWDYT GVLTSVRYGT 

       730        740        750        760        770        780 
GANEATDQGS EATKTQGMAV ITSNNPSLKL NQNDKVIVNM GTAHKNQEYR PLLLTTKDGL 

       790        800        810        820        830        840 
TSYTSDAAAK SLYRKTNDKG ELVFDASDIQ GYLNPQVSGY LAVWVPVGAS DNQDVRVAAS 

       850        860        870        880        890        900 
NKANATGQVY ESSSALDSQL IYEGFSNFQD FVTKDSDYTN KKIAQNVQLF KSWGVTSFEM 

       910        920        930        940        950        960 
APQYVSSEDG SFLDSIIQNG YAFEDRYDLA MSKNNKYGSQ QDMINAVKAL HKSGIQVIAD 

       970        980        990       1000       1010       1020 
WVPDQIYNLP GKEVVTATRV NDYGEYRKDS EIKNTLYAAN TKSNGKDYQA KYGGAFLSEL 

      1030       1040       1050       1060       1070       1080 
AAKYPSIFNR TQISNGKKID PSEKITAWKA KYFNGTNILG RGVGYVLKDN ASDKYFELKG 

      1090       1100       1110       1120       1130       1140 
NQTYLPKQMT NKEASTGFVN DGNGMTFYST SGYQAKNSFV QDAKGNWYYF DNNGHMVYGL 

      1150       1160       1170       1180       1190       1200 
QHLNGEVQYF LSNGVQLRES FLENADGSKN YFGHLGNRYS NGYYSFDNDS KWRYFDASGV 

      1210       1220       1230       1240       1250       1260 
MAVGLKTING NTQYFDQDGY QVKGAWITGS DGKKRYFDDG SGNMAVNRFA NDKNGDWYYL 

      1270       1280       1290       1300       1310       1320 
NSDGIALVGV QTINGKTYYF GQDGKQIKGK IITDNGKLKY FLANSGELAR NIFATDSQNN 

      1330       1340       1350       1360       1370       1380 
WYYFGSDGVA VTGSQTIAGK KLYFASDGKQ VKGSFVTYNG KVHYYHADSG ELQVNRFEAD 

      1390       1400       1410       1420       1430       1440 
KDGNWYYLDS NGEALTGSQR INGQRVFFTR EGKQVKGDVA YDERGLLRYY DKNSGNMVYN 

      1450       1460 
KVVTLANGRR IGIDRWGIAR YY 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the Streptococcus mutans gtfD gene encoding the glucosyltransferase-S enzyme."
Honda O., Kato C., Kuramitsu H.K.
J. Gen. Microbiol. 136:2099-2105(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: GS-5.
[2]"Molecular analyses of glucosyltransferase genes among strains of Streptococcus mutans."
Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S., Kimura S., Hamada S.
FEMS Microbiol. Lett. 161:331-336(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f, MT4467 / Serotype e and MT8148 / Serotype c.
[3]"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen."
Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J.
Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700610 / UA159.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M29296 Genomic DNA. Translation: AAA26895.1.
D88653 Genomic DNA. Translation: BAA26103.1.
D88656 Genomic DNA. Translation: BAA26107.1.
D88659 Genomic DNA. Translation: BAA26111.1.
D88662 Genomic DNA. Translation: BAA26115.1.
D89979 Genomic DNA. Translation: BAA26121.1.
AE014133 Genomic DNA. Translation: AAN58619.1.
RefSeqNP_721313.1. NC_004350.2.

3D structure databases

ProteinModelPortalP49331.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING210007.SMU.910.

Protein family/group databases

CAZyGH70. Glycoside Hydrolase Family 70.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN58619; AAN58619; SMU_910.
GeneID1028270.
KEGGsmu:SMU_910.
PATRIC19663961. VBIStrMut61772_0813.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG5263.
KOK00689.
OMAKDHLQGG.
OrthoDBEOG60PH6T.
PhylomeDBP49331.

Enzyme and pathway databases

BioCycSMUT210007:GC7Z-869-MONOMER.

Family and domain databases

Gene3D3.20.20.80. 2 hits.
InterProIPR018337. Cell_wall/Cho-bd_repeat.
IPR027636. Glucan-bd_rpt.
IPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR022263. KxYKxGKxW.
[Graphical view]
PfamPF01473. CW_binding_1. 5 hits.
PF02324. Glyco_hydro_70. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 3 hits.
TIGRFAMsTIGR04035. glucan_65_rpt. 5 hits.
TIGR03715. KxYKxGKxW. 1 hit.
PROSITEPS51170. CW. 14 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGTFD_STRMU
AccessionPrimary (citable) accession number: P49331
Secondary accession number(s): O69383 expand/collapse secondary AC list , O69386, O69389, O69392, O69398
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 28, 2002
Last modified: July 9, 2014
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries