Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P49331

- GTFD_STRMU

UniProt

P49331 - GTFD_STRMU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glucosyltransferase-S

Gene

gtfD

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

Catalytic activityi

Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).

GO - Molecular functioni

  1. dextransucrase activity Source: UniProtKB-EC
  2. glucosyltransferase activity Source: InterPro

GO - Biological processi

  1. glucan biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciSMUT210007:GC7Z-869-MONOMER.
SABIO-RKP49331.

Protein family/group databases

CAZyiGH70. Glycoside Hydrolase Family 70.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosyltransferase-S (EC:2.4.1.5)
Short name:
GTF-S
Alternative name(s):
Dextransucrase
Sucrose 6-glucosyltransferase
Gene namesi
Name:gtfD
Ordered Locus Names:SMU_910
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002512: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Keywords - Diseasei

Dental caries

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 1462Glucosyltransferase-SPRO_0000021387
Signal peptidei1 – ?Sequence Analysis

Interactioni

Protein-protein interaction databases

STRINGi210007.SMU.910.

Structurei

3D structure databases

ProteinModelPortaliP49331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati171 – 19020Cell wall-binding 1Add
BLAST
Repeati192 – 21120Cell wall-binding 2Add
BLAST
Repeati1095 – 111521Cell wall-binding 3Add
BLAST
Repeati1116 – 113621Cell wall-binding 4Add
BLAST
Repeati1137 – 115620Cell wall-binding 5Add
BLAST
Repeati1180 – 120122Cell wall-binding 6Add
BLAST
Repeati1202 – 122120Cell wall-binding 7Add
BLAST
Repeati1223 – 124422Cell wall-binding 8Add
BLAST
Repeati1246 – 126621Cell wall-binding 9Add
BLAST
Repeati1267 – 128620Cell wall-binding 10Add
BLAST
Repeati1310 – 133021Cell wall-binding 11Add
BLAST
Repeati1331 – 135020Cell wall-binding 12Add
BLAST
Repeati1352 – 137221Cell wall-binding 13Add
BLAST
Repeati1374 – 139421Cell wall-binding 14Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 70 family.Curated
Contains 14 cell wall-binding repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG5263.
KOiK00689.
OMAiKDHLQGG.
OrthoDBiEOG60PH6T.
PhylomeDBiP49331.

Family and domain databases

Gene3Di3.20.20.80. 2 hits.
InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR027636. Glucan-bd_rpt.
IPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR022263. KxYKxGKxW.
[Graphical view]
PfamiPF01473. CW_binding_1. 5 hits.
PF02324. Glyco_hydro_70. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 3 hits.
TIGRFAMsiTIGR04035. glucan_65_rpt. 5 hits.
TIGR03715. KxYKxGKxW. 1 hit.
PROSITEiPS51170. CW. 14 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49331-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
METKRRYKMY KVKKHWVTIA VASGLITLGT TTLGSSVSAE TEQQTSDKVV
60 70 80 90 100
TQKSEDDKAA SESSQTDAPK TKQAQTEQTQ AQSQANVADT STSITKETPS
110 120 130 140 150
QNITTQANSD DKTVTNTKSE EAQTSEERTK QAEEAQATAS SQALTQAKAE
160 170 180 190 200
LTKQRQTAAQ ENKNPVDLAA IPNVKQIDGK YYYIGSDGQP KKNFALTVNN
210 220 230 240 250
KVLYFDKNTG ALTDTSQYQF KQGLTKLNND YTPHNQIVNF ENTSLETIDN
260 270 280 290 300
YVTADSWYRP KDILKNGKTW TASSESDLRP LLMSWWPDKQ TQIAYLNYMN
310 320 330 340 350
QQGLGTGENY TADSSQESLN LAAQTVQVKI ETKISQTQQT QWLRDIINSF
360 370 380 390 400
VKTQPNWNSQ TESDTSAGEK DHLQGGALLY SNSDKTAYAN SDYRLLNRTP
410 420 430 440 450
TSQTGKPKYF EDNSSGGYDF LLANDIDNSN PVVQAEQLNW LHYLMNYGSI
460 470 480 490 500
VANDPEANFD GVRVDAVDNV NADLLQIASD YLKAHYGVDK SEKNAINHLS
510 520 530 540 550
ILEAWSDNDP QYNKDTKGAQ LPIDNKLRLS LLYALTRPLE KDASNKNEIR
560 570 580 590 600
SGLEPVITNS LNNRSAEGKN SERMANYIFI RAHDSEVQTV IAKIIKAQIN
610 620 630 640 650
PKTDGLTFTL DELKQAFKIY NEDMRQAKKK YTQSNIPTAY ALMLSNKDSI
660 670 680 690 700
TRLYYGDMYS DDGQYMATKS PYYDAIDTLL KARIKYAAGG QDMKITYVEG
710 720 730 740 750
DKSHMDWDYT GVLTSVRYGT GANEATDQGS EATKTQGMAV ITSNNPSLKL
760 770 780 790 800
NQNDKVIVNM GTAHKNQEYR PLLLTTKDGL TSYTSDAAAK SLYRKTNDKG
810 820 830 840 850
ELVFDASDIQ GYLNPQVSGY LAVWVPVGAS DNQDVRVAAS NKANATGQVY
860 870 880 890 900
ESSSALDSQL IYEGFSNFQD FVTKDSDYTN KKIAQNVQLF KSWGVTSFEM
910 920 930 940 950
APQYVSSEDG SFLDSIIQNG YAFEDRYDLA MSKNNKYGSQ QDMINAVKAL
960 970 980 990 1000
HKSGIQVIAD WVPDQIYNLP GKEVVTATRV NDYGEYRKDS EIKNTLYAAN
1010 1020 1030 1040 1050
TKSNGKDYQA KYGGAFLSEL AAKYPSIFNR TQISNGKKID PSEKITAWKA
1060 1070 1080 1090 1100
KYFNGTNILG RGVGYVLKDN ASDKYFELKG NQTYLPKQMT NKEASTGFVN
1110 1120 1130 1140 1150
DGNGMTFYST SGYQAKNSFV QDAKGNWYYF DNNGHMVYGL QHLNGEVQYF
1160 1170 1180 1190 1200
LSNGVQLRES FLENADGSKN YFGHLGNRYS NGYYSFDNDS KWRYFDASGV
1210 1220 1230 1240 1250
MAVGLKTING NTQYFDQDGY QVKGAWITGS DGKKRYFDDG SGNMAVNRFA
1260 1270 1280 1290 1300
NDKNGDWYYL NSDGIALVGV QTINGKTYYF GQDGKQIKGK IITDNGKLKY
1310 1320 1330 1340 1350
FLANSGELAR NIFATDSQNN WYYFGSDGVA VTGSQTIAGK KLYFASDGKQ
1360 1370 1380 1390 1400
VKGSFVTYNG KVHYYHADSG ELQVNRFEAD KDGNWYYLDS NGEALTGSQR
1410 1420 1430 1440 1450
INGQRVFFTR EGKQVKGDVA YDERGLLRYY DKNSGNMVYN KVVTLANGRR
1460
IGIDRWGIAR YY
Length:1,462
Mass (Da):163,388
Last modified:November 28, 2002 - v3
Checksum:iCE4A279C4D708645
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1428 – 146235RYYDK…IARYY → VYR in AAA26895. (PubMed:2148600)CuratedAdd
BLAST

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti10 – 101Y → H in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
Natural varianti19 – 191I → V in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
Natural varianti58 – 581K → E in strain: MT4467.
Natural varianti68 – 681A → S in strain: MT4239 and MT4245.
Natural varianti81 – 811A → T in strain: MT4251 and MT8148.
Natural varianti113 – 1131T → I in strain: MT4239 and MT4245.
Natural varianti122 – 1221A → V in strain: MT4239, MT4245 and MT8148.
Natural varianti132 – 1321A → S in strain: GS-5 and MT4467.
Natural varianti135 – 1351A → V in strain: MT4245.
Natural varianti137 – 1371A → T in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
Natural varianti202 – 2021V → L in strain: MT4239.
Natural varianti255 – 2551D → N in strain: MT8148.
Natural varianti275 – 2751E → D in strain: MT4239, MT4245 and MT4251.
Natural varianti288 – 2881D → N in strain: MT4239, MT4245 and MT4251.
Natural varianti301 – 3011Q → H in strain: MT4245.
Natural varianti313 – 3131D → N in strain: MT4239 and MT4251.
Natural varianti317 – 3171E → K in strain: MT4239.
Natural varianti328 – 3281V → F in strain: MT4239.
Natural varianti350 – 3501F → L in strain: MT4239, MT4251 and MT4467.
Natural varianti628 – 6336KKKYTQ → EKEYTL in strain: MT4251.
Natural varianti688 – 6881A → S in strain: MT4239.
Natural varianti726 – 7327TDQGSEA → ADKGNDS in strain: MT4251.
Natural varianti726 – 7305TDQGS → ADKGN in strain: MT4239 and MT4245.
Natural varianti762 – 7621T → A in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
Natural varianti964 – 9641D → Y in strain: MT4251.
Natural varianti1019 – 10191E → K in strain: MT4245 and MT4251.
Natural varianti1059 – 10602LG → IR in strain: MT4251.
Natural varianti1060 – 10601G → R in strain: MT4245.
Natural varianti1080 – 10801G → R in strain: MT4239.
Natural varianti1142 – 11421H → Q in strain: GS-5.
Natural varianti1198 – 11981S → N in strain: MT4239.
Natural varianti1220 – 12201Y → C in strain: MT4251 and MT4467.
Natural varianti1280 – 12801F → L in strain: MT4467.
Natural varianti1282 – 12821Q → P in strain: MT4245.
Natural varianti1290 – 12901K → T in strain: MT4245.
Natural varianti1311 – 13111N → D in strain: MT4245.
Natural varianti1403 – 14031G → D in strain: GS-5 and MT4467.
Natural varianti1425 – 14251G → R in strain: GS-5.
Natural varianti1449 – 14491R → K in strain: MT4467.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29296 Genomic DNA. Translation: AAA26895.1.
D88653 Genomic DNA. Translation: BAA26103.1.
D88656 Genomic DNA. Translation: BAA26107.1.
D88659 Genomic DNA. Translation: BAA26111.1.
D88662 Genomic DNA. Translation: BAA26115.1.
D89979 Genomic DNA. Translation: BAA26121.1.
AE014133 Genomic DNA. Translation: AAN58619.1.
RefSeqiNP_721313.1. NC_004350.2.
WP_002352262.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN58619; AAN58619; SMU_910.
GeneIDi1028270.
KEGGismu:SMU_910.
PATRICi19663961. VBIStrMut61772_0813.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29296 Genomic DNA. Translation: AAA26895.1 .
D88653 Genomic DNA. Translation: BAA26103.1 .
D88656 Genomic DNA. Translation: BAA26107.1 .
D88659 Genomic DNA. Translation: BAA26111.1 .
D88662 Genomic DNA. Translation: BAA26115.1 .
D89979 Genomic DNA. Translation: BAA26121.1 .
AE014133 Genomic DNA. Translation: AAN58619.1 .
RefSeqi NP_721313.1. NC_004350.2.
WP_002352262.1. NC_004350.2.

3D structure databases

ProteinModelPortali P49331.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 210007.SMU.910.

Protein family/group databases

CAZyi GH70. Glycoside Hydrolase Family 70.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN58619 ; AAN58619 ; SMU_910 .
GeneIDi 1028270.
KEGGi smu:SMU_910.
PATRICi 19663961. VBIStrMut61772_0813.

Phylogenomic databases

eggNOGi COG5263.
KOi K00689.
OMAi KDHLQGG.
OrthoDBi EOG60PH6T.
PhylomeDBi P49331.

Enzyme and pathway databases

BioCyci SMUT210007:GC7Z-869-MONOMER.
SABIO-RK P49331.

Family and domain databases

Gene3Di 3.20.20.80. 2 hits.
InterProi IPR018337. Cell_wall/Cho-bd_repeat.
IPR027636. Glucan-bd_rpt.
IPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR022263. KxYKxGKxW.
[Graphical view ]
Pfami PF01473. CW_binding_1. 5 hits.
PF02324. Glyco_hydro_70. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 3 hits.
TIGRFAMsi TIGR04035. glucan_65_rpt. 5 hits.
TIGR03715. KxYKxGKxW. 1 hit.
PROSITEi PS51170. CW. 14 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Streptococcus mutans gtfD gene encoding the glucosyltransferase-S enzyme."
    Honda O., Kato C., Kuramitsu H.K.
    J. Gen. Microbiol. 136:2099-2105(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: GS-5.
  2. "Molecular analyses of glucosyltransferase genes among strains of Streptococcus mutans."
    Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S., Kimura S., Hamada S.
    FEMS Microbiol. Lett. 161:331-336(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f, MT4467 / Serotype e and MT8148 / Serotype c.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700610 / UA159.

Entry informationi

Entry nameiGTFD_STRMU
AccessioniPrimary (citable) accession number: P49331
Secondary accession number(s): O69383
, O69386, O69389, O69392, O69398
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 28, 2002
Last modified: November 26, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3