Reviewed, UniProtKB/Swiss-Prot P49331 (GTFD_STRMU)

Names and origin

Protein names

Recommended name:
    Glucosyltransferase-S
      Short name=GTF-S
    EC=2.4.1.5
Alternative name(s):
    Dextransucrase
    Sucrose 6-glucosyltransferase

Gene names

Name:	gtfD
Ordered Locus Names:	SMU_910

Organism

Streptococcus mutans [Complete proteome] [HAMAP]

Taxonomic identifier

1309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	1462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.
Catalytic activity	Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).
Subcellular location	Secreted.
Miscellaneous	GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 70 family. Contains 14 cell wall-binding repeats.

Ontologies

Keywords
Cellular component	Secreted
Disease	Dental caries
Domain	Repeat Signal
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucan biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro dextransucrase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Chain	? – 1462		Glucosyltransferase-S		PRO_0000021387
Regions
	Repeat	171 – 190	20	Cell wall-binding 1
	Repeat	192 – 211	20	Cell wall-binding 2
	Repeat	1095 – 1115	21	Cell wall-binding 3
	Repeat	1116 – 1136	21	Cell wall-binding 4
	Repeat	1137 – 1156	20	Cell wall-binding 5
	Repeat	1180 – 1201	22	Cell wall-binding 6
	Repeat	1202 – 1221	20	Cell wall-binding 7
	Repeat	1223 – 1244	22	Cell wall-binding 8
	Repeat	1246 – 1266	21	Cell wall-binding 9
	Repeat	1267 – 1286	20	Cell wall-binding 10
	Repeat	1310 – 1330	21	Cell wall-binding 11
	Repeat	1331 – 1350	20	Cell wall-binding 12
	Repeat	1352 – 1372	21	Cell wall-binding 13
	Repeat	1374 – 1394	21	Cell wall-binding 14
Natural variations
	Natural variant	10	1	Y → H in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
	Natural variant	19	1	I → V in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
	Natural variant	58	1	K → E in strain: MT4467.
	Natural variant	68	1	A → S in strain: MT4239 and MT4245.
	Natural variant	81	1	A → T in strain: MT4251 and MT8148.
	Natural variant	113	1	T → I in strain: MT4239 and MT4245.
	Natural variant	122	1	A → V in strain: MT4239, MT4245 and MT8148.
	Natural variant	132	1	A → S in strain: GS-5 and MT4467.
	Natural variant	135	1	A → V in strain: MT4245.
	Natural variant	137	1	A → T in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
	Natural variant	202	1	V → L in strain: MT4239.
	Natural variant	255	1	D → N in strain: MT8148.
	Natural variant	275	1	E → D in strain: MT4239, MT4245 and MT4251.
	Natural variant	288	1	D → N in strain: MT4239, MT4245 and MT4251.
	Natural variant	301	1	Q → H in strain: MT4245.
	Natural variant	313	1	D → N in strain: MT4239 and MT4251.
	Natural variant	317	1	E → K in strain: MT4239.
	Natural variant	328	1	V → F in strain: MT4239.
	Natural variant	350	1	F → L in strain: MT4239, MT4251 and MT4467.
	Natural variant	628 – 633	6	KKKYTQ → EKEYTL in strain: MT4251.
	Natural variant	688	1	A → S in strain: MT4239.
	Natural variant	726 – 732	7	TDQGSEA → ADKGNDS in strain: MT4251.
	Natural variant	726 – 730	5	TDQGS → ADKGN in strain: MT4239 and MT4245.
	Natural variant	762	1	T → A in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
	Natural variant	964	1	D → Y in strain: MT4251.
	Natural variant	1019	1	E → K in strain: MT4245 and MT4251.
	Natural variant	1059 – 1060	2	LG → IR in strain: MT4251.
	Natural variant	1060	1	G → R in strain: MT4245.
	Natural variant	1080	1	G → R in strain: MT4239.
	Natural variant	1142	1	H → Q in strain: GS-5.
	Natural variant	1198	1	S → N in strain: MT4239.
	Natural variant	1220	1	Y → C in strain: MT4251 and MT4467.
	Natural variant	1280	1	F → L in strain: MT4467.
	Natural variant	1282	1	Q → P in strain: MT4245.
	Natural variant	1290	1	K → T in strain: MT4245.
	Natural variant	1311	1	N → D in strain: MT4245.
	Natural variant	1403	1	G → D in strain: GS-5 and MT4467.
	Natural variant	1425	1	G → R in strain: GS-5.
	Natural variant	1449	1	R → K in strain: MT4467.
Experimental info
	Sequence conflict	1428 – 1462	35	RYYDK…IARYY → VYR in AAA26895. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49331-1 [UniParc]. Last modified November 28, 2002. Version 3. Checksum: CE4A279C4D708645	FASTA	1,462	163,388
	10 20 30 40 50 60 METKRRYKMY KVKKHWVTIA VASGLITLGT TTLGSSVSAE TEQQTSDKVV TQKSEDDKAA 70 80 90 100 110 120 SESSQTDAPK TKQAQTEQTQ AQSQANVADT STSITKETPS QNITTQANSD DKTVTNTKSE 130 140 150 160 170 180 EAQTSEERTK QAEEAQATAS SQALTQAKAE LTKQRQTAAQ ENKNPVDLAA IPNVKQIDGK 190 200 210 220 230 240 YYYIGSDGQP KKNFALTVNN KVLYFDKNTG ALTDTSQYQF KQGLTKLNND YTPHNQIVNF 250 260 270 280 290 300 ENTSLETIDN YVTADSWYRP KDILKNGKTW TASSESDLRP LLMSWWPDKQ TQIAYLNYMN 310 320 330 340 350 360 QQGLGTGENY TADSSQESLN LAAQTVQVKI ETKISQTQQT QWLRDIINSF VKTQPNWNSQ 370 380 390 400 410 420 TESDTSAGEK DHLQGGALLY SNSDKTAYAN SDYRLLNRTP TSQTGKPKYF EDNSSGGYDF 430 440 450 460 470 480 LLANDIDNSN PVVQAEQLNW LHYLMNYGSI VANDPEANFD GVRVDAVDNV NADLLQIASD 490 500 510 520 530 540 YLKAHYGVDK SEKNAINHLS ILEAWSDNDP QYNKDTKGAQ LPIDNKLRLS LLYALTRPLE 550 560 570 580 590 600 KDASNKNEIR SGLEPVITNS LNNRSAEGKN SERMANYIFI RAHDSEVQTV IAKIIKAQIN 610 620 630 640 650 660 PKTDGLTFTL DELKQAFKIY NEDMRQAKKK YTQSNIPTAY ALMLSNKDSI TRLYYGDMYS 670 680 690 700 710 720 DDGQYMATKS PYYDAIDTLL KARIKYAAGG QDMKITYVEG DKSHMDWDYT GVLTSVRYGT 730 740 750 760 770 780 GANEATDQGS EATKTQGMAV ITSNNPSLKL NQNDKVIVNM GTAHKNQEYR PLLLTTKDGL 790 800 810 820 830 840 TSYTSDAAAK SLYRKTNDKG ELVFDASDIQ GYLNPQVSGY LAVWVPVGAS DNQDVRVAAS 850 860 870 880 890 900 NKANATGQVY ESSSALDSQL IYEGFSNFQD FVTKDSDYTN KKIAQNVQLF KSWGVTSFEM 910 920 930 940 950 960 APQYVSSEDG SFLDSIIQNG YAFEDRYDLA MSKNNKYGSQ QDMINAVKAL HKSGIQVIAD 970 980 990 1000 1010 1020 WVPDQIYNLP GKEVVTATRV NDYGEYRKDS EIKNTLYAAN TKSNGKDYQA KYGGAFLSEL 1030 1040 1050 1060 1070 1080 AAKYPSIFNR TQISNGKKID PSEKITAWKA KYFNGTNILG RGVGYVLKDN ASDKYFELKG 1090 1100 1110 1120 1130 1140 NQTYLPKQMT NKEASTGFVN DGNGMTFYST SGYQAKNSFV QDAKGNWYYF DNNGHMVYGL 1150 1160 1170 1180 1190 1200 QHLNGEVQYF LSNGVQLRES FLENADGSKN YFGHLGNRYS NGYYSFDNDS KWRYFDASGV 1210 1220 1230 1240 1250 1260 MAVGLKTING NTQYFDQDGY QVKGAWITGS DGKKRYFDDG SGNMAVNRFA NDKNGDWYYL 1270 1280 1290 1300 1310 1320 NSDGIALVGV QTINGKTYYF GQDGKQIKGK IITDNGKLKY FLANSGELAR NIFATDSQNN 1330 1340 1350 1360 1370 1380 WYYFGSDGVA VTGSQTIAGK KLYFASDGKQ VKGSFVTYNG KVHYYHADSG ELQVNRFEAD 1390 1400 1410 1420 1430 1440 KDGNWYYLDS NGEALTGSQR INGQRVFFTR EGKQVKGDVA YDERGLLRYY DKNSGNMVYN 1450 1460 KVVTLANGRR IGIDRWGIAR YY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the Streptococcus mutans gtfD gene encoding the glucosyltransferase-S enzyme." Honda O., Kato C., Kuramitsu H.K. J. Gen. Microbiol. 136:2099-2105(1990) [PubMed: 2148600] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GS-5.
[2]	"Molecular analyses of glucosyltransferase genes among strains of Streptococcus mutans." Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S., Kimura S., Hamada S. FEMS Microbiol. Lett. 161:331-336(1998) [PubMed: 9570124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f, MT4467 / Serotype e and MT8148 / Serotype c.
[3]	"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen." Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J. Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed: 12397186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700610 / UA159 / Serotype c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29296 Genomic DNA. Translation: AAA26895.1. D88653 Genomic DNA. Translation: BAA26103.1. D88656 Genomic DNA. Translation: BAA26107.1. D88659 Genomic DNA. Translation: BAA26111.1. D88662 Genomic DNA. Translation: BAA26115.1. D89979 Genomic DNA. Translation: BAA26121.1. AE014133 Genomic DNA. Translation: AAN58619.1.
RefSeq	NP_721313.1.
3D structure databases
SMR	P49331. Positions 856-984, 1093-1394, 1124-1443.
ModBase	Search...
Protein family/group databases
CAZy	GH70. Glycoside Hydrolase Family 70.
Genome annotation databases
GeneID	1028270.
GenomeReviews	Gene locus SMU_910 in contig AE014133_GR.
KEGG	smu:SMU.910.
NMPDR	fig|210007.1.peg.826.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG532029.
OMA	IRYFDAN.
Enzyme and pathway databases
BioCyc	SMUT210007:SMU_910-MONOMER.
BRENDA	2.4.1.5. 20716.
Family and domain databases
InterPro	IPR018337. Cell_wall/Cho-bd_repeat. IPR002479. Cell_wall_bd_put. IPR003318. Glyco_hydro70cat. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
Pfam	PF01473. CW_binding_1. 5 hits. PF02324. Glyco_hydro_70. 1 hit. [Graphical view]
PROSITE	PS51170. CW. 14 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GTFD_STRMU

Accession

Primary (citable) accession number: P49331
Secondary accession number(s): O69383 , O69386, O69389, O69392, O69398

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 28, 2002
Last modified:	January 19, 2010
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)