Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P49331

- GTFD_STRMU

UniProt

P49331 - GTFD_STRMU

Protein

Glucosyltransferase-S

Gene

gtfD

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 3 (28 Nov 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

    Catalytic activityi

    Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).

    GO - Molecular functioni

    1. dextransucrase activity Source: UniProtKB-EC
    2. glucosyltransferase activity Source: InterPro

    GO - Biological processi

    1. glucan biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciSMUT210007:GC7Z-869-MONOMER.

    Protein family/group databases

    CAZyiGH70. Glycoside Hydrolase Family 70.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosyltransferase-S (EC:2.4.1.5)
    Short name:
    GTF-S
    Alternative name(s):
    Dextransucrase
    Sucrose 6-glucosyltransferase
    Gene namesi
    Name:gtfD
    Ordered Locus Names:SMU_910
    OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
    Taxonomic identifieri210007 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000002512: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Keywords - Diseasei

    Dental caries

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 1462Glucosyltransferase-SPRO_0000021387
    Signal peptidei1 – ?Sequence Analysis

    Interactioni

    Protein-protein interaction databases

    STRINGi210007.SMU.910.

    Structurei

    3D structure databases

    ProteinModelPortaliP49331.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati171 – 19020Cell wall-binding 1Add
    BLAST
    Repeati192 – 21120Cell wall-binding 2Add
    BLAST
    Repeati1095 – 111521Cell wall-binding 3Add
    BLAST
    Repeati1116 – 113621Cell wall-binding 4Add
    BLAST
    Repeati1137 – 115620Cell wall-binding 5Add
    BLAST
    Repeati1180 – 120122Cell wall-binding 6Add
    BLAST
    Repeati1202 – 122120Cell wall-binding 7Add
    BLAST
    Repeati1223 – 124422Cell wall-binding 8Add
    BLAST
    Repeati1246 – 126621Cell wall-binding 9Add
    BLAST
    Repeati1267 – 128620Cell wall-binding 10Add
    BLAST
    Repeati1310 – 133021Cell wall-binding 11Add
    BLAST
    Repeati1331 – 135020Cell wall-binding 12Add
    BLAST
    Repeati1352 – 137221Cell wall-binding 13Add
    BLAST
    Repeati1374 – 139421Cell wall-binding 14Add
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 70 family.Curated
    Contains 14 cell wall-binding repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG5263.
    KOiK00689.
    OMAiKDHLQGG.
    OrthoDBiEOG60PH6T.
    PhylomeDBiP49331.

    Family and domain databases

    Gene3Di3.20.20.80. 2 hits.
    InterProiIPR018337. Cell_wall/Cho-bd_repeat.
    IPR027636. Glucan-bd_rpt.
    IPR003318. Glyco_hydro70cat.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR022263. KxYKxGKxW.
    [Graphical view]
    PfamiPF01473. CW_binding_1. 5 hits.
    PF02324. Glyco_hydro_70. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 3 hits.
    TIGRFAMsiTIGR04035. glucan_65_rpt. 5 hits.
    TIGR03715. KxYKxGKxW. 1 hit.
    PROSITEiPS51170. CW. 14 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49331-1 [UniParc]FASTAAdd to Basket

    « Hide

    METKRRYKMY KVKKHWVTIA VASGLITLGT TTLGSSVSAE TEQQTSDKVV     50
    TQKSEDDKAA SESSQTDAPK TKQAQTEQTQ AQSQANVADT STSITKETPS 100
    QNITTQANSD DKTVTNTKSE EAQTSEERTK QAEEAQATAS SQALTQAKAE 150
    LTKQRQTAAQ ENKNPVDLAA IPNVKQIDGK YYYIGSDGQP KKNFALTVNN 200
    KVLYFDKNTG ALTDTSQYQF KQGLTKLNND YTPHNQIVNF ENTSLETIDN 250
    YVTADSWYRP KDILKNGKTW TASSESDLRP LLMSWWPDKQ TQIAYLNYMN 300
    QQGLGTGENY TADSSQESLN LAAQTVQVKI ETKISQTQQT QWLRDIINSF 350
    VKTQPNWNSQ TESDTSAGEK DHLQGGALLY SNSDKTAYAN SDYRLLNRTP 400
    TSQTGKPKYF EDNSSGGYDF LLANDIDNSN PVVQAEQLNW LHYLMNYGSI 450
    VANDPEANFD GVRVDAVDNV NADLLQIASD YLKAHYGVDK SEKNAINHLS 500
    ILEAWSDNDP QYNKDTKGAQ LPIDNKLRLS LLYALTRPLE KDASNKNEIR 550
    SGLEPVITNS LNNRSAEGKN SERMANYIFI RAHDSEVQTV IAKIIKAQIN 600
    PKTDGLTFTL DELKQAFKIY NEDMRQAKKK YTQSNIPTAY ALMLSNKDSI 650
    TRLYYGDMYS DDGQYMATKS PYYDAIDTLL KARIKYAAGG QDMKITYVEG 700
    DKSHMDWDYT GVLTSVRYGT GANEATDQGS EATKTQGMAV ITSNNPSLKL 750
    NQNDKVIVNM GTAHKNQEYR PLLLTTKDGL TSYTSDAAAK SLYRKTNDKG 800
    ELVFDASDIQ GYLNPQVSGY LAVWVPVGAS DNQDVRVAAS NKANATGQVY 850
    ESSSALDSQL IYEGFSNFQD FVTKDSDYTN KKIAQNVQLF KSWGVTSFEM 900
    APQYVSSEDG SFLDSIIQNG YAFEDRYDLA MSKNNKYGSQ QDMINAVKAL 950
    HKSGIQVIAD WVPDQIYNLP GKEVVTATRV NDYGEYRKDS EIKNTLYAAN 1000
    TKSNGKDYQA KYGGAFLSEL AAKYPSIFNR TQISNGKKID PSEKITAWKA 1050
    KYFNGTNILG RGVGYVLKDN ASDKYFELKG NQTYLPKQMT NKEASTGFVN 1100
    DGNGMTFYST SGYQAKNSFV QDAKGNWYYF DNNGHMVYGL QHLNGEVQYF 1150
    LSNGVQLRES FLENADGSKN YFGHLGNRYS NGYYSFDNDS KWRYFDASGV 1200
    MAVGLKTING NTQYFDQDGY QVKGAWITGS DGKKRYFDDG SGNMAVNRFA 1250
    NDKNGDWYYL NSDGIALVGV QTINGKTYYF GQDGKQIKGK IITDNGKLKY 1300
    FLANSGELAR NIFATDSQNN WYYFGSDGVA VTGSQTIAGK KLYFASDGKQ 1350
    VKGSFVTYNG KVHYYHADSG ELQVNRFEAD KDGNWYYLDS NGEALTGSQR 1400
    INGQRVFFTR EGKQVKGDVA YDERGLLRYY DKNSGNMVYN KVVTLANGRR 1450
    IGIDRWGIAR YY 1462
    Length:1,462
    Mass (Da):163,388
    Last modified:November 28, 2002 - v3
    Checksum:iCE4A279C4D708645
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1428 – 146235RYYDK…IARYY → VYR in AAA26895. (PubMed:2148600)CuratedAdd
    BLAST

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti10 – 101Y → H in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
    Natural varianti19 – 191I → V in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
    Natural varianti58 – 581K → E in strain: MT4467.
    Natural varianti68 – 681A → S in strain: MT4239 and MT4245.
    Natural varianti81 – 811A → T in strain: MT4251 and MT8148.
    Natural varianti113 – 1131T → I in strain: MT4239 and MT4245.
    Natural varianti122 – 1221A → V in strain: MT4239, MT4245 and MT8148.
    Natural varianti132 – 1321A → S in strain: GS-5 and MT4467.
    Natural varianti135 – 1351A → V in strain: MT4245.
    Natural varianti137 – 1371A → T in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
    Natural varianti202 – 2021V → L in strain: MT4239.
    Natural varianti255 – 2551D → N in strain: MT8148.
    Natural varianti275 – 2751E → D in strain: MT4239, MT4245 and MT4251.
    Natural varianti288 – 2881D → N in strain: MT4239, MT4245 and MT4251.
    Natural varianti301 – 3011Q → H in strain: MT4245.
    Natural varianti313 – 3131D → N in strain: MT4239 and MT4251.
    Natural varianti317 – 3171E → K in strain: MT4239.
    Natural varianti328 – 3281V → F in strain: MT4239.
    Natural varianti350 – 3501F → L in strain: MT4239, MT4251 and MT4467.
    Natural varianti628 – 6336KKKYTQ → EKEYTL in strain: MT4251.
    Natural varianti688 – 6881A → S in strain: MT4239.
    Natural varianti726 – 7327TDQGSEA → ADKGNDS in strain: MT4251.
    Natural varianti726 – 7305TDQGS → ADKGN in strain: MT4239 and MT4245.
    Natural varianti762 – 7621T → A in strain: GS-5, MT4239, MT4245, MT4251, MT4467 and MT8148.
    Natural varianti964 – 9641D → Y in strain: MT4251.
    Natural varianti1019 – 10191E → K in strain: MT4245 and MT4251.
    Natural varianti1059 – 10602LG → IR in strain: MT4251.
    Natural varianti1060 – 10601G → R in strain: MT4245.
    Natural varianti1080 – 10801G → R in strain: MT4239.
    Natural varianti1142 – 11421H → Q in strain: GS-5.
    Natural varianti1198 – 11981S → N in strain: MT4239.
    Natural varianti1220 – 12201Y → C in strain: MT4251 and MT4467.
    Natural varianti1280 – 12801F → L in strain: MT4467.
    Natural varianti1282 – 12821Q → P in strain: MT4245.
    Natural varianti1290 – 12901K → T in strain: MT4245.
    Natural varianti1311 – 13111N → D in strain: MT4245.
    Natural varianti1403 – 14031G → D in strain: GS-5 and MT4467.
    Natural varianti1425 – 14251G → R in strain: GS-5.
    Natural varianti1449 – 14491R → K in strain: MT4467.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29296 Genomic DNA. Translation: AAA26895.1.
    D88653 Genomic DNA. Translation: BAA26103.1.
    D88656 Genomic DNA. Translation: BAA26107.1.
    D88659 Genomic DNA. Translation: BAA26111.1.
    D88662 Genomic DNA. Translation: BAA26115.1.
    D89979 Genomic DNA. Translation: BAA26121.1.
    AE014133 Genomic DNA. Translation: AAN58619.1.
    RefSeqiNP_721313.1. NC_004350.2.

    Genome annotation databases

    EnsemblBacteriaiAAN58619; AAN58619; SMU_910.
    GeneIDi1028270.
    KEGGismu:SMU_910.
    PATRICi19663961. VBIStrMut61772_0813.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29296 Genomic DNA. Translation: AAA26895.1 .
    D88653 Genomic DNA. Translation: BAA26103.1 .
    D88656 Genomic DNA. Translation: BAA26107.1 .
    D88659 Genomic DNA. Translation: BAA26111.1 .
    D88662 Genomic DNA. Translation: BAA26115.1 .
    D89979 Genomic DNA. Translation: BAA26121.1 .
    AE014133 Genomic DNA. Translation: AAN58619.1 .
    RefSeqi NP_721313.1. NC_004350.2.

    3D structure databases

    ProteinModelPortali P49331.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 210007.SMU.910.

    Protein family/group databases

    CAZyi GH70. Glycoside Hydrolase Family 70.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN58619 ; AAN58619 ; SMU_910 .
    GeneIDi 1028270.
    KEGGi smu:SMU_910.
    PATRICi 19663961. VBIStrMut61772_0813.

    Phylogenomic databases

    eggNOGi COG5263.
    KOi K00689.
    OMAi KDHLQGG.
    OrthoDBi EOG60PH6T.
    PhylomeDBi P49331.

    Enzyme and pathway databases

    BioCyci SMUT210007:GC7Z-869-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 2 hits.
    InterProi IPR018337. Cell_wall/Cho-bd_repeat.
    IPR027636. Glucan-bd_rpt.
    IPR003318. Glyco_hydro70cat.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR022263. KxYKxGKxW.
    [Graphical view ]
    Pfami PF01473. CW_binding_1. 5 hits.
    PF02324. Glyco_hydro_70. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 3 hits.
    TIGRFAMsi TIGR04035. glucan_65_rpt. 5 hits.
    TIGR03715. KxYKxGKxW. 1 hit.
    PROSITEi PS51170. CW. 14 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the Streptococcus mutans gtfD gene encoding the glucosyltransferase-S enzyme."
      Honda O., Kato C., Kuramitsu H.K.
      J. Gen. Microbiol. 136:2099-2105(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: GS-5.
    2. "Molecular analyses of glucosyltransferase genes among strains of Streptococcus mutans."
      Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S., Kimura S., Hamada S.
      FEMS Microbiol. Lett. 161:331-336(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f, MT4467 / Serotype e and MT8148 / Serotype c.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700610 / UA159.

    Entry informationi

    Entry nameiGTFD_STRMU
    AccessioniPrimary (citable) accession number: P49331
    Secondary accession number(s): O69383
    , O69386, O69389, O69392, O69398
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: November 28, 2002
    Last modified: October 1, 2014
    This is version 113 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3